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The map label for this gene is pyrG

Identifier: 29346000

GI number: 29346000

Start: 729633

End: 731246

Strand: Reverse

Name: pyrG

Synonym: BT_0590

Alternate gene names: 29346000

Gene position: 731246-729633 (Counterclockwise)

Preceding gene: 29346002

Following gene: 29345999

Centisome position: 11.68

GC content: 47.96

Gene sequence:

>1614_bases
GTGGGAGAAACAAAGTATATTTTCGTCACCGGTGGTGTTGCCTCTTCTTTAGGAAAAGGGATCATCTCATCCTCCATCGG
TAAATTGCTGCAAGCAAGAGGTTACAATGTAACCATCCAGAAGTTTGACCCGTATATCAACATCGACCCGGGAACATTGA
ATCCTTATGAGCATGGAGAATGCTATGTGACCGTAGACGGCCATGAAGCCGATCTTGACCTGGGACACTACGAACGTTTC
CTGGGTATTCAGACTACAAAGGCTAACAACATCACTACGGGACGTATCTACAAAAGTGTTATTGACAAAGAACGTCGCGG
AGACTATCTGGGTAAGACGATTCAGGTGATTCCTCATATCACGGATGAAATCAAACGGAACGTAAAATTGCTCGGTAACA
AATACAAGTTCGATTTTGTAATTACCGAAATCGGTGGTACGGTAGGTGATATCGAATCACTCCCCTACCTCGAAAGTATC
CGCCAGTTGAAATGGGAGTTGGGTAAGAACGCTCTTTGCGTACACCTGACATATGTGCCTTATCTTGCCGCTGCCGGAGA
GCTGAAAACCAAACCGACACAGCATTCTGTCAAGGAACTCCAGAGCGTAGGTATTCAGCCGGACGTATTGGTGCTTCGTG
CCGAACACCCGCTGAGCGACGGACTCCGCAAAAAAGTAGCTCAATTCTGTAATGTGGATGACAAGGCTGTCGTGCAGTCT
ATCGACGCAGAAACAATTTATGAAGTACCTCTCCTGATGCAGGCACAGGGACTGGACAGCACAATCCTTGAAAAGATGGG
GCTGCCCGTAGGAGAAACTCCGGGACTAGGTCCGTGGCGTAAATTCCTGGAACGCCGTCATGCTGCTGAAACGAAAGAAC
CCATCAACATCGCATTGGTAGGTAAATATGACTTGCAGGATGCTTACAAGTCAATCCGTGAAGCATTGTCACAGGCAGGT
ACTTACAACGACCGCAAAGTGGAGGTTCACTTTGTGAACAGCGAAAAACTGACAGATGAGAACGTAGCCGAAGCCTTGAA
AGGCATGGCAGGCGTGATGATCGGTCCGGGATTCGGTCAGCGTGGTATCGACGGTAAGTTCGTTGCCATCAAATATACAC
GTACACATGATATACCGACCTTCGGTATCTGTCTGGGTATGCAATGTATCGCTATCGAATTTGCCCGCAACGTATTGGGA
TATGCCGATGCCGACTCACGCGAAATGGATGAAAAGACTCCGCACAACGTGATCGACATCATGGAGGAACAGAAAGCGAT
CACCAATATGGGAGGTACGATGCGTCTGGGCGCTTACGAATGTGTGCTTCAGAAAGGATCGAAAGCATACCTTGCTTATG
GTGAAGAACATATTCAAGAACGTCACCGCCATCGCTATGAGTTCAACAATGACTATAAGGCTCAATACGAAGCTGCCGGT
ATGAAATGCGTGGGTATCAACCCCGAATCGGATCTGGTGGAAATCGTTGAGATCCCGGCATTGAAGTGGTTTATCGGCAC
TCAGTTCCATCCGGAATACAGCAGTACGGTATTGAACCCGCATCCGTTGTTTGTGGCTTTCGTGAAGGCAGCCATCGAGA
ACGAGAAGAATTGA

Upstream 100 bases:

>100_bases
TAGAGGGGAAAGTCTGCATTCTTCCCCTTTTTTTTATAATTTTGCGCTTTTTAAGTTCGTAAGAAACTACTAAGTAAAAA
TAATTAAAACAGTTATAGCT

Downstream 100 bases:

>100_bases
CAATTGACAGTTGACAATTGACAATTTATTATTCAAAAATTTATAATTCATAATTAATAGGAATGGATAAAAACACCATT
ACAGGTCTCGTTTTAATAGG

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase

Number of amino acids: Translated: 537; Mature: 536

Protein sequence:

>537_residues
MGETKYIFVTGGVASSLGKGIISSSIGKLLQARGYNVTIQKFDPYINIDPGTLNPYEHGECYVTVDGHEADLDLGHYERF
LGIQTTKANNITTGRIYKSVIDKERRGDYLGKTIQVIPHITDEIKRNVKLLGNKYKFDFVITEIGGTVGDIESLPYLESI
RQLKWELGKNALCVHLTYVPYLAAAGELKTKPTQHSVKELQSVGIQPDVLVLRAEHPLSDGLRKKVAQFCNVDDKAVVQS
IDAETIYEVPLLMQAQGLDSTILEKMGLPVGETPGLGPWRKFLERRHAAETKEPINIALVGKYDLQDAYKSIREALSQAG
TYNDRKVEVHFVNSEKLTDENVAEALKGMAGVMIGPGFGQRGIDGKFVAIKYTRTHDIPTFGICLGMQCIAIEFARNVLG
YADADSREMDEKTPHNVIDIMEEQKAITNMGGTMRLGAYECVLQKGSKAYLAYGEEHIQERHRHRYEFNNDYKAQYEAAG
MKCVGINPESDLVEIVEIPALKWFIGTQFHPEYSSTVLNPHPLFVAFVKAAIENEKN

Sequences:

>Translated_537_residues
MGETKYIFVTGGVASSLGKGIISSSIGKLLQARGYNVTIQKFDPYINIDPGTLNPYEHGECYVTVDGHEADLDLGHYERF
LGIQTTKANNITTGRIYKSVIDKERRGDYLGKTIQVIPHITDEIKRNVKLLGNKYKFDFVITEIGGTVGDIESLPYLESI
RQLKWELGKNALCVHLTYVPYLAAAGELKTKPTQHSVKELQSVGIQPDVLVLRAEHPLSDGLRKKVAQFCNVDDKAVVQS
IDAETIYEVPLLMQAQGLDSTILEKMGLPVGETPGLGPWRKFLERRHAAETKEPINIALVGKYDLQDAYKSIREALSQAG
TYNDRKVEVHFVNSEKLTDENVAEALKGMAGVMIGPGFGQRGIDGKFVAIKYTRTHDIPTFGICLGMQCIAIEFARNVLG
YADADSREMDEKTPHNVIDIMEEQKAITNMGGTMRLGAYECVLQKGSKAYLAYGEEHIQERHRHRYEFNNDYKAQYEAAG
MKCVGINPESDLVEIVEIPALKWFIGTQFHPEYSSTVLNPHPLFVAFVKAAIENEKN
>Mature_536_residues
GETKYIFVTGGVASSLGKGIISSSIGKLLQARGYNVTIQKFDPYINIDPGTLNPYEHGECYVTVDGHEADLDLGHYERFL
GIQTTKANNITTGRIYKSVIDKERRGDYLGKTIQVIPHITDEIKRNVKLLGNKYKFDFVITEIGGTVGDIESLPYLESIR
QLKWELGKNALCVHLTYVPYLAAAGELKTKPTQHSVKELQSVGIQPDVLVLRAEHPLSDGLRKKVAQFCNVDDKAVVQSI
DAETIYEVPLLMQAQGLDSTILEKMGLPVGETPGLGPWRKFLERRHAAETKEPINIALVGKYDLQDAYKSIREALSQAGT
YNDRKVEVHFVNSEKLTDENVAEALKGMAGVMIGPGFGQRGIDGKFVAIKYTRTHDIPTFGICLGMQCIAIEFARNVLGY
ADADSREMDEKTPHNVIDIMEEQKAITNMGGTMRLGAYECVLQKGSKAYLAYGEEHIQERHRHRYEFNNDYKAQYEAAGM
KCVGINPESDLVEIVEIPALKWFIGTQFHPEYSSTVLNPHPLFVAFVKAAIENEKN

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI148491070, Length=550, Percent_Identity=43.0909090909091, Blast_Score=445, Evalue=1e-125,
Organism=Homo sapiens, GI28559085, Length=552, Percent_Identity=43.1159420289855, Blast_Score=441, Evalue=1e-124,
Organism=Homo sapiens, GI28559083, Length=552, Percent_Identity=43.1159420289855, Blast_Score=441, Evalue=1e-124,
Organism=Homo sapiens, GI221316689, Length=552, Percent_Identity=43.1159420289855, Blast_Score=441, Evalue=1e-124,
Organism=Escherichia coli, GI1789142, Length=545, Percent_Identity=48.4403669724771, Blast_Score=499, Evalue=1e-142,
Organism=Caenorhabditis elegans, GI25148299, Length=609, Percent_Identity=39.4088669950739, Blast_Score=391, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6322563, Length=563, Percent_Identity=39.7868561278863, Blast_Score=396, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6319432, Length=562, Percent_Identity=40.7473309608541, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24664469, Length=552, Percent_Identity=43.6594202898551, Blast_Score=432, Evalue=1e-121,
Organism=Drosophila melanogaster, GI21357815, Length=498, Percent_Identity=41.1646586345382, Blast_Score=359, Evalue=3e-99,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRG_BACTN (Q8AA75)

Other databases:

- EMBL:   AE015928
- RefSeq:   NP_809503.1
- HSSP:   P17812
- ProteinModelPortal:   Q8AA75
- SMR:   Q8AA75
- GeneID:   1072187
- GenomeReviews:   AE015928_GR
- KEGG:   bth:BT_0590
- NMPDR:   fig|226186.1.peg.590
- HOGENOM:   HBG597806
- OMA:   RVTMQKL
- ProtClustDB:   PRK05380
- BioCyc:   BTHE226186:BT_0590-MONOMER
- BRENDA:   6.3.4.2
- HAMAP:   MF_01227
- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991
- TIGRFAMs:   TIGR00337

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase

EC number: =6.3.4.2

Molecular weight: Translated: 59865; Mature: 59734

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 384-384 ACT_SITE 510-510 ACT_SITE 512-512

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGETKYIFVTGGVASSLGKGIISSSIGKLLQARGYNVTIQKFDPYINIDPGTLNPYEHGE
CCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEECCCCCCCCCCCE
CYVTVDGHEADLDLGHYERFLGIQTTKANNITTGRIYKSVIDKERRGDYLGKTIQVIPHI
EEEEECCCCCCCCHHHHHHHHCEEECCCCCCCHHHHHHHHHHHHHCCCHHCCHHHHHHHH
TDEIKRNVKLLGNKYKFDFVITEIGGTVGDIESLPYLESIRQLKWELGKNALCVHLTYVP
HHHHHHHHHHHCCEEEEEEEEEECCCCCCCHHCCHHHHHHHHHHHHHCCCEEEEEEEHHH
YLAAAGELKTKPTQHSVKELQSVGIQPDVLVLRAEHPLSDGLRKKVAQFCNVDDKAVVQS
HHHHCCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCHHHHHHH
IDAETIYEVPLLMQAQGLDSTILEKMGLPVGETPGLGPWRKFLERRHAAETKEPINIALV
CCHHHHHHCCHHEECCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEE
GKYDLQDAYKSIREALSQAGTYNDRKVEVHFVNSEKLTDENVAEALKGMAGVMIGPGFGQ
ECCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCEEECCCCCC
RGIDGKFVAIKYTRTHDIPTFGICLGMQCIAIEFARNVLGYADADSREMDEKTPHNVIDI
CCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCHHHHHH
MEEQKAITNMGGTMRLGAYECVLQKGSKAYLAYGEEHIQERHRHRYEFNNDYKAQYEAAG
HHHHHHHHHCCCCEEECHHHHHHHCCCCEEEECCHHHHHHHHHHCCCCCCCCCCEECCCC
MKCVGINPESDLVEIVEIPALKWFIGTQFHPEYSSTVLNPHPLFVAFVKAAIENEKN
EEEEECCCCHHHHHHHHHCCEEHHHCCCCCCCHHCCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GETKYIFVTGGVASSLGKGIISSSIGKLLQARGYNVTIQKFDPYINIDPGTLNPYEHGE
CCEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEECCCCCCCCCCCE
CYVTVDGHEADLDLGHYERFLGIQTTKANNITTGRIYKSVIDKERRGDYLGKTIQVIPHI
EEEEECCCCCCCCHHHHHHHHCEEECCCCCCCHHHHHHHHHHHHHCCCHHCCHHHHHHHH
TDEIKRNVKLLGNKYKFDFVITEIGGTVGDIESLPYLESIRQLKWELGKNALCVHLTYVP
HHHHHHHHHHHCCEEEEEEEEEECCCCCCCHHCCHHHHHHHHHHHHHCCCEEEEEEEHHH
YLAAAGELKTKPTQHSVKELQSVGIQPDVLVLRAEHPLSDGLRKKVAQFCNVDDKAVVQS
HHHHCCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCHHHHHHH
IDAETIYEVPLLMQAQGLDSTILEKMGLPVGETPGLGPWRKFLERRHAAETKEPINIALV
CCHHHHHHCCHHEECCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEE
GKYDLQDAYKSIREALSQAGTYNDRKVEVHFVNSEKLTDENVAEALKGMAGVMIGPGFGQ
ECCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCEEECCCCCC
RGIDGKFVAIKYTRTHDIPTFGICLGMQCIAIEFARNVLGYADADSREMDEKTPHNVIDI
CCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCHHHHHH
MEEQKAITNMGGTMRLGAYECVLQKGSKAYLAYGEEHIQERHRHRYEFNNDYKAQYEAAG
HHHHHHHHHCCCCEEECHHHHHHHCCCCEEEECCHHHHHHHHHHCCCCCCCCCCEECCCC
MKCVGINPESDLVEIVEIPALKWFIGTQFHPEYSSTVLNPHPLFVAFVKAAIENEKN
EEEEECCCCHHHHHHHHHCCEEHHHCCCCCCCHHCCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12663928