Click here to switch to the map view.

The map label for this gene is purH [H]

Identifier: 29345928

GI number: 29345928

Start: 640445

End: 641620

Strand: Reverse

Name: purH [H]

Synonym: BT_0518

Alternate gene names: 29345928

Gene position: 641620-640445 (Counterclockwise)

Preceding gene: 29345931

Following gene: 29345927

Centisome position: 10.25

GC content: 45.41

Gene sequence:

>1176_bases
ATGGCAAACGAACTCGAACTGAAATACGGCTGCAACCCAAATCAAAAGCCTGCCCGTATCTTCATGAAAGAAGGCGAACT
ACCCATTGAGGTATTAAACGGACGTCCCGGTTACATCAATCTGCTGGATGCATTCAACAGTTGGCAGTTAGTTAAAGAAC
TAAAAGAAGCTACCGGACTTCCGGCTGCCGCATCATTCAAACACGTCAGCCCGGCAGGTGCTGCTGTGGCTGTAGAAATG
AGTGACACTCTGAAGAAAATCTATTTTGTGGATGATGTAAAACTGTCTCCACTGGCAACAGCCTATGCACGCGCACGTGG
TGCCGACCGTATGTCTTCTTACGGAGACTTCATCGCATTGTCCGATACTTGTGATGAAGAGACTGCACGCATTATCAACC
GTGAAGTATCCGATGGTGTGATTGCACCGGATTACACTCCCGAAGCTCTGGAAATCCTGAAAAATAAAAGAAAAGGAACT
TACAATGTGATCAAGATAGATCCGGCTTACCGCCCGGCTCCTATTGAACACAAAGATGTTTTCGGAGTGACTTTTGAGCA
AGGCAGAAACGAACTGAAAATAGACGAAAGTCTTTTGAAGGAAATGCCCACACAAAACAAGGAAATTCCAGCTGAAGCCA
AACGTGACCTAATCATTTCTTTAATCACCTTAAAATATACACAATCCAATTCCGTATGTTATGCAAAAGACGGACAAGCG
ATTGGTATCGGTGCAGGACAACAGTCACGCATTCACTGCACGCGCCTTGCAGGAAATAAAGCAGATATCTGGTATCTCCG
CCAACATCCGAAAGTGATGAATCTGCCTTGGATTGAAAAAATCCGCCGTGCAGATCGTGATAATACTATCGACGTTTATA
TTTCAGAAGATCATGATGACGTATTGGCAGACGGCGTTTGGCAACAGTTTTTTACAGAAAAGCCGGAAGTCCTGACCCGC
GAAGAGAAACGTGCATGGCTGGATACAATGACTGGCGTAGCTTTAGGCTCTGACGCATTCTTCCCCTTCGGAGACAATAT
CGAACGTGCACACAAAAGCGGTGTAAGCTATATCGCACAACCGGGAGGTTCTGTACGAGATGATCATGTTATCGGTACAT
GCGACAAGTACAATATGGCAATGGCATTCACAGGCATTCGCTTGTTCCATCATTAA

Upstream 100 bases:

>100_bases
TTACTTGTTACTAATTTATAACAGGAGGCTAGGCTGGCTATTCTTTTTTTAGTAATTTTGTCGCTATCAATTTGTAATCA
GTAATTCATAATTAAATAAT

Downstream 100 bases:

>100_bases
TCGTTTTCTGCTATATTTTAAGATTTATATCAGGTTTGTTGGCTTTAACCTATTTATAGATCCGAAACGTTATGCTATCC
CCATTTATAATGAGAATAAA

Product: 5-aminoimidazole-4-carboxamide ribonucleotide transformylase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MANELELKYGCNPNQKPARIFMKEGELPIEVLNGRPGYINLLDAFNSWQLVKELKEATGLPAAASFKHVSPAGAAVAVEM
SDTLKKIYFVDDVKLSPLATAYARARGADRMSSYGDFIALSDTCDEETARIINREVSDGVIAPDYTPEALEILKNKRKGT
YNVIKIDPAYRPAPIEHKDVFGVTFEQGRNELKIDESLLKEMPTQNKEIPAEAKRDLIISLITLKYTQSNSVCYAKDGQA
IGIGAGQQSRIHCTRLAGNKADIWYLRQHPKVMNLPWIEKIRRADRDNTIDVYISEDHDDVLADGVWQQFFTEKPEVLTR
EEKRAWLDTMTGVALGSDAFFPFGDNIERAHKSGVSYIAQPGGSVRDDHVIGTCDKYNMAMAFTGIRLFHH

Sequences:

>Translated_391_residues
MANELELKYGCNPNQKPARIFMKEGELPIEVLNGRPGYINLLDAFNSWQLVKELKEATGLPAAASFKHVSPAGAAVAVEM
SDTLKKIYFVDDVKLSPLATAYARARGADRMSSYGDFIALSDTCDEETARIINREVSDGVIAPDYTPEALEILKNKRKGT
YNVIKIDPAYRPAPIEHKDVFGVTFEQGRNELKIDESLLKEMPTQNKEIPAEAKRDLIISLITLKYTQSNSVCYAKDGQA
IGIGAGQQSRIHCTRLAGNKADIWYLRQHPKVMNLPWIEKIRRADRDNTIDVYISEDHDDVLADGVWQQFFTEKPEVLTR
EEKRAWLDTMTGVALGSDAFFPFGDNIERAHKSGVSYIAQPGGSVRDDHVIGTCDKYNMAMAFTGIRLFHH
>Mature_390_residues
ANELELKYGCNPNQKPARIFMKEGELPIEVLNGRPGYINLLDAFNSWQLVKELKEATGLPAAASFKHVSPAGAAVAVEMS
DTLKKIYFVDDVKLSPLATAYARARGADRMSSYGDFIALSDTCDEETARIINREVSDGVIAPDYTPEALEILKNKRKGTY
NVIKIDPAYRPAPIEHKDVFGVTFEQGRNELKIDESLLKEMPTQNKEIPAEAKRDLIISLITLKYTQSNSVCYAKDGQAI
GIGAGQQSRIHCTRLAGNKADIWYLRQHPKVMNLPWIEKIRRADRDNTIDVYISEDHDDVLADGVWQQFFTEKPEVLTRE
EKRAWLDTMTGVALGSDAFFPFGDNIERAHKSGVSYIAQPGGSVRDDHVIGTCDKYNMAMAFTGIRLFHH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=395, Percent_Identity=54.6835443037975, Blast_Score=458, Evalue=1e-129,
Organism=Escherichia coli, GI1790439, Length=266, Percent_Identity=30.0751879699248, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71985564, Length=392, Percent_Identity=54.3367346938776, Blast_Score=416, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI71985556, Length=420, Percent_Identity=48.0952380952381, Blast_Score=395, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI71985574, Length=296, Percent_Identity=57.0945945945946, Blast_Score=319, Evalue=1e-87,
Organism=Saccharomyces cerevisiae, GI6323056, Length=390, Percent_Identity=55.3846153846154, Blast_Score=456, Evalue=1e-129,
Organism=Saccharomyces cerevisiae, GI6323768, Length=393, Percent_Identity=54.70737913486, Blast_Score=446, Evalue=1e-126,
Organism=Drosophila melanogaster, GI24649832, Length=391, Percent_Identity=57.2890025575448, Blast_Score=473, Evalue=1e-133,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 43772; Mature: 43641

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANELELKYGCNPNQKPARIFMKEGELPIEVLNGRPGYINLLDAFNSWQLVKELKEATGL
CCCCEEEEECCCCCCCCCEEEEECCCCCEEEECCCCCEEEHHHHCCHHHHHHHHHHHCCC
PAAASFKHVSPAGAAVAVEMSDTLKKIYFVDDVKLSPLATAYARARGADRMSSYGDFIAL
CCCCCCCCCCCCCCEEEEEEHHHHEEEEEECCCCCCHHHHHHHHHCCHHHHHCCCCEEEE
SDTCDEETARIINREVSDGVIAPDYTPEALEILKNKRKGTYNVIKIDPAYRPAPIEHKDV
CCCCCHHHHHHHHHHCCCCEECCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCE
FGVTFEQGRNELKIDESLLKEMPTQNKEIPAEAKRDLIISLITLKYTQSNSVCYAKDGQA
EEEEHHCCCCCEEECHHHHHHCCCCCCCCCHHHHHHHEEEEEEEEEECCCCEEEECCCCE
IGIGAGQQSRIHCTRLAGNKADIWYLRQHPKVMNLPWIEKIRRADRDNTIDVYISEDHDD
EEECCCCCCCEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHCCCCCEEEEEEECCCCH
VLADGVWQQFFTEKPEVLTREEKRAWLDTMTGVALGSDAFFPFGDNIERAHKSGVSYIAQ
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCHHHHHHCCCCEEEC
PGGSVRDDHVIGTCDKYNMAMAFTGIRLFHH
CCCCCCCCCEEECCCCCCEEEEEEEEEEECC
>Mature Secondary Structure 
ANELELKYGCNPNQKPARIFMKEGELPIEVLNGRPGYINLLDAFNSWQLVKELKEATGL
CCCEEEEECCCCCCCCCEEEEECCCCCEEEECCCCCEEEHHHHCCHHHHHHHHHHHCCC
PAAASFKHVSPAGAAVAVEMSDTLKKIYFVDDVKLSPLATAYARARGADRMSSYGDFIAL
CCCCCCCCCCCCCCEEEEEEHHHHEEEEEECCCCCCHHHHHHHHHCCHHHHHCCCCEEEE
SDTCDEETARIINREVSDGVIAPDYTPEALEILKNKRKGTYNVIKIDPAYRPAPIEHKDV
CCCCCHHHHHHHHHHCCCCEECCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCE
FGVTFEQGRNELKIDESLLKEMPTQNKEIPAEAKRDLIISLITLKYTQSNSVCYAKDGQA
EEEEHHCCCCCEEECHHHHHHCCCCCCCCCHHHHHHHEEEEEEEEEECCCCEEEECCCCE
IGIGAGQQSRIHCTRLAGNKADIWYLRQHPKVMNLPWIEKIRRADRDNTIDVYISEDHDD
EEECCCCCCCEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHCCCCCEEEEEEECCCCH
VLADGVWQQFFTEKPEVLTREEKRAWLDTMTGVALGSDAFFPFGDNIERAHKSGVSYIAQ
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCHHHHHHCCCCEEEC
PGGSVRDDHVIGTCDKYNMAMAFTGIRLFHH
CCCCCCCCCEEECCCCCCEEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA