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The map label for this gene is araD [H]

Identifier: 29345763

GI number: 29345763

Start: 425643

End: 426326

Strand: Reverse

Name: araD [H]

Synonym: BT_0353

Alternate gene names: 29345763

Gene position: 426326-425643 (Counterclockwise)

Preceding gene: 29345764

Following gene: 29345762

Centisome position: 6.81

GC content: 47.37

Gene sequence:

>684_bases
ATGCTGGAAGAACTGAAAGAAAAAGTATTTCATGCCAATCTCGAATTGGTAAAGCATGGATTAGTCATCTTTACATGGGG
AAATGTTTCTGCCATCGACCGTGAAACGGAACTGGTAGTCATCAAACCCAGTGGAGTCAGTTATGATGATATGAAAGCGG
AAGATATGGTAGTAGTGGACCTGGATGGCAAAGTCGTCGAAGGACGGCTGAAGCCATCCTCAGATACTCCTACTCACGTG
GTACTCTACAAAGCATTCCCCGAAATCGGCGGAGTGGTGCATACCCACTCTACTTATGCTACCGCATGGGCGCAGGCCGG
TTGTGATATTCCCAATATCGGGACAACTCACGCTGACTATTTCCATGATGCAATCCCCTGCACAGCGGATATGACGGAAG
CTGAAGTAAAAGGTGCCTATGAACTGGAAACCGGAAACGTGATCGTAAAACGTTTCGAAGGCCTGAACCCTGTACATACA
CCGGGAGTATTGGTCAAGAATCATGGTCCTTTCTCTTGGGGAAAAGACGCACACGATGCCGTACACAATGCAGTAGTGAT
GGAACAAGTTGCCAAAATGGCAAGCATTGCTTATGCCGTGAATCCCAATTTAACAATGAACCCGCTGCTGGTAGAGAAAC
ACTTCAGCCGCAAGCATGGTCCGAACGCTTACTACGGACAATAA

Upstream 100 bases:

>100_bases
CGGATAAAATCGATAAACTGGGTTCCAAACGGGGTGCAGCCTTGTATAAATTCAACGGCAAAGCCTACCGCAAGGACCCG
AAATTTAAATTATAATCAAT

Downstream 100 bases:

>100_bases
AATAATTGACAATTGACAGTTGACAATTGACAATTAATGCGAGAGGACATGAAGGATAATGTAGTAAAAGATAAAAGTTT
GGAGTTCGCTGTTCGGATTG

Product: L-ribulose-5-phosphate 4-epimerase

Products: NA

Alternate protein names: L-ascorbate utilization protein F; Phosphoribulose isomerase [H]

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MLEELKEKVFHANLELVKHGLVIFTWGNVSAIDRETELVVIKPSGVSYDDMKAEDMVVVDLDGKVVEGRLKPSSDTPTHV
VLYKAFPEIGGVVHTHSTYATAWAQAGCDIPNIGTTHADYFHDAIPCTADMTEAEVKGAYELETGNVIVKRFEGLNPVHT
PGVLVKNHGPFSWGKDAHDAVHNAVVMEQVAKMASIAYAVNPNLTMNPLLVEKHFSRKHGPNAYYGQ

Sequences:

>Translated_227_residues
MLEELKEKVFHANLELVKHGLVIFTWGNVSAIDRETELVVIKPSGVSYDDMKAEDMVVVDLDGKVVEGRLKPSSDTPTHV
VLYKAFPEIGGVVHTHSTYATAWAQAGCDIPNIGTTHADYFHDAIPCTADMTEAEVKGAYELETGNVIVKRFEGLNPVHT
PGVLVKNHGPFSWGKDAHDAVHNAVVMEQVAKMASIAYAVNPNLTMNPLLVEKHFSRKHGPNAYYGQ
>Mature_227_residues
MLEELKEKVFHANLELVKHGLVIFTWGNVSAIDRETELVVIKPSGVSYDDMKAEDMVVVDLDGKVVEGRLKPSSDTPTHV
VLYKAFPEIGGVVHTHSTYATAWAQAGCDIPNIGTTHADYFHDAIPCTADMTEAEVKGAYELETGNVIVKRFEGLNPVHT
PGVLVKNHGPFSWGKDAHDAVHNAVVMEQVAKMASIAYAVNPNLTMNPLLVEKHFSRKHGPNAYYGQ

Specific function: Catalyzes the isomerization of L-ribulose 5-phosphate to D-xylulose 5-phosphate. Is involved in the anaerobic L-ascorbate utilization [H]

COG id: COG0235

COG function: function code G; Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldolase class II family. AraD/FucA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790642, Length=227, Percent_Identity=66.079295154185, Blast_Score=322, Evalue=1e-89,
Organism=Escherichia coli, GI1786247, Length=231, Percent_Identity=61.9047619047619, Blast_Score=288, Evalue=2e-79,
Organism=Escherichia coli, GI1790008, Length=231, Percent_Identity=59.7402597402597, Blast_Score=280, Evalue=8e-77,
Organism=Escherichia coli, GI1789164, Length=124, Percent_Identity=33.0645161290323, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001303 [H]

Pfam domain/function: PF00596 Aldolase_II [H]

EC number: =5.1.3.4 [H]

Molecular weight: Translated: 24888; Mature: 24888

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEELKEKVFHANLELVKHGLVIFTWGNVSAIDRETELVVIKPSGVSYDDMKAEDMVVVD
CHHHHHHHHHHHHHHHHHCCEEEEEECCEEEECCCCEEEEECCCCCCCCCCCCCCEEEEE
LDGKVVEGRLKPSSDTPTHVVLYKAFPEIGGVVHTHSTYATAWAQAGCDIPNIGTTHADY
CCCEEEEEEECCCCCCCCEEEEEECCCCCCCEEEECCHHHHHHHHCCCCCCCCCCCCHHH
FHDAIPCTADMTEAEVKGAYELETGNVIVKRFEGLNPVHTPGVLVKNHGPFSWGKDAHDA
HHCCCCCCCCCCHHHCCCEEEEECCCEEEEECCCCCCCCCCCEEEECCCCCCCCCCHHHH
VHNAVVMEQVAKMASIAYAVNPNLTMNPLLVEKHFSRKHGPNAYYGQ
HHHHHHHHHHHHHHHEEEEECCCCEECHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MLEELKEKVFHANLELVKHGLVIFTWGNVSAIDRETELVVIKPSGVSYDDMKAEDMVVVD
CHHHHHHHHHHHHHHHHHCCEEEEEECCEEEECCCCEEEEECCCCCCCCCCCCCCEEEEE
LDGKVVEGRLKPSSDTPTHVVLYKAFPEIGGVVHTHSTYATAWAQAGCDIPNIGTTHADY
CCCEEEEEEECCCCCCCCEEEEEECCCCCCCEEEECCHHHHHHHHCCCCCCCCCCCCHHH
FHDAIPCTADMTEAEVKGAYELETGNVIVKRFEGLNPVHTPGVLVKNHGPFSWGKDAHDA
HHCCCCCCCCCCHHHCCCEEEEECCCEEEEECCCCCCCCCCCEEEECCCCCCCCCCHHHH
VHNAVVMEQVAKMASIAYAVNPNLTMNPLLVEKHFSRKHGPNAYYGQ
HHHHHHHHHHHHHHHEEEEECCCCEECHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA