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The map label for this gene is afr [H]

Identifier: 29345732

GI number: 29345732

Start: 388527

End: 389501

Strand: Reverse

Name: afr [H]

Synonym: BT_0322

Alternate gene names: 29345732

Gene position: 389501-388527 (Counterclockwise)

Preceding gene: 29345733

Following gene: 29345731

Centisome position: 6.22

GC content: 48.1

Gene sequence:

>975_bases
ATGATTAAATGGGGCTTCATCGGCTGCGGAGAAGTAACCAAAACCAAAAGTGGCCCCGCCTTTCAAAAGGTAGAACATTC
TGAAGTAGTAGCTGTAATGAGCCGTGATGGCGCCAAAGCAAAGGCTTATGCGAAAGAAAGAGGAATCAGAAAATGGTATG
ATGACGCTCAGGAACTGATAGACGATCCGGAAGTAAATGCCATTTATATCGCCACTCCTCCTTCTTCACACGCTACATAC
GCCATTATGGCTATGAAAGCAGGCAAGCCTGCCTATATTGAAAAACCAATGGCTGTCACTTATGAGGAGTGTACACGCAT
CAACCGTATTTCTAAGGAAACGGGTGTTCCCTGTTTCGTCGCATATTACCGCCGCTATCTCCCCTACTTTCAGAAAGTGA
AAGAAATGGTAGAGAACGGTTCTATCGGTAATGTCATAAACGTACAAGTCCGCTTTGCACAGCCGCCACGTGATTTGGAT
TACAACCGTGAAAATCTTCCTTGGCGTGTACAGGCTGATATTGCCGGTGGCGGTTACTTCTATGACCTCGCCCCGCATCA
GATCGACTTGCTGCAAGATATGTTCGGCTGCATCCTCGAAGCCAGCGGATATAAGAGCAATCGTGGAGGGCTTTATCCTG
CCGAAGATACGCTGAGCGCCTGCTTCCAGTTCGACAACGGATTGGTCGGCAGTGGTTCATGGTGCTTTGTTGCCCATGAT
TCCGCCCGCGAAGACCGCATCGAAATTATCGGAGATAAAGGTATGATCTGCTTCTCTGTCTTTACGTACGAGCCGATTGG
TCTGCACACCGAACGCGGCCGGGAGGAAATCTGTATCGGTAATCCTGAGCATGTTCAACAACCTCTTATTCAGGCAGTAG
TAGATCATCTGCTCGGTAAGTCAGTCTGCTCATGCGACGGAGAAAGTGCTACCTTGACTAACTGGGTAATGGACAAAATA
TTAGGGAAGCTCTAA

Upstream 100 bases:

>100_bases
TTCTGACAGTTAACAATCAATAAAGTTGTTCTTAATAAAAAATAGCTTTATCTTTGGGAGTTGTTAAAACAAGCACAATA
GGTATATTATGAGTGAAAAG

Downstream 100 bases:

>100_bases
GATAATGCTCCCTCGTTTGTTTCTTAAAATAATTTAATCACTTTATTCTATCGTTAGTTTTCTTTATATTTGCGAAAGGT
AGCAATAAAAGCAAAACTAA

Product: putative oxidoreductase

Products: NA

Alternate protein names: Anhydrofructose reductase; 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming) [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MIKWGFIGCGEVTKTKSGPAFQKVEHSEVVAVMSRDGAKAKAYAKERGIRKWYDDAQELIDDPEVNAIYIATPPSSHATY
AIMAMKAGKPAYIEKPMAVTYEECTRINRISKETGVPCFVAYYRRYLPYFQKVKEMVENGSIGNVINVQVRFAQPPRDLD
YNRENLPWRVQADIAGGGYFYDLAPHQIDLLQDMFGCILEASGYKSNRGGLYPAEDTLSACFQFDNGLVGSGSWCFVAHD
SAREDRIEIIGDKGMICFSVFTYEPIGLHTERGREEICIGNPEHVQQPLIQAVVDHLLGKSVCSCDGESATLTNWVMDKI
LGKL

Sequences:

>Translated_324_residues
MIKWGFIGCGEVTKTKSGPAFQKVEHSEVVAVMSRDGAKAKAYAKERGIRKWYDDAQELIDDPEVNAIYIATPPSSHATY
AIMAMKAGKPAYIEKPMAVTYEECTRINRISKETGVPCFVAYYRRYLPYFQKVKEMVENGSIGNVINVQVRFAQPPRDLD
YNRENLPWRVQADIAGGGYFYDLAPHQIDLLQDMFGCILEASGYKSNRGGLYPAEDTLSACFQFDNGLVGSGSWCFVAHD
SAREDRIEIIGDKGMICFSVFTYEPIGLHTERGREEICIGNPEHVQQPLIQAVVDHLLGKSVCSCDGESATLTNWVMDKI
LGKL
>Mature_324_residues
MIKWGFIGCGEVTKTKSGPAFQKVEHSEVVAVMSRDGAKAKAYAKERGIRKWYDDAQELIDDPEVNAIYIATPPSSHATY
AIMAMKAGKPAYIEKPMAVTYEECTRINRISKETGVPCFVAYYRRYLPYFQKVKEMVENGSIGNVINVQVRFAQPPRDLD
YNRENLPWRVQADIAGGGYFYDLAPHQIDLLQDMFGCILEASGYKSNRGGLYPAEDTLSACFQFDNGLVGSGSWCFVAHD
SAREDRIEIIGDKGMICFSVFTYEPIGLHTERGREEICIGNPEHVQQPLIQAVVDHLLGKSVCSCDGESATLTNWVMDKI
LGKL

Specific function: Catalyzes the NADPH-specific reduction of 1,5-anhydro-D- fructose to 1,5-anhydro-D-mannitol. Also shows some activity against structurally related compounds such as 3-keto-1,5-anhydro- D-fructose, D-glucosone and D-xylosone. The enzyme cannot use NADH as

COG id: COG0673

COG function: function code R; Predicted dehydrogenases and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gfo/idh/mocA family [H]

Homologues:

Organism=Homo sapiens, GI7657212, Length=265, Percent_Identity=24.1509433962264, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI87081947, Length=199, Percent_Identity=29.6482412060301, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1789848, Length=199, Percent_Identity=28.643216080402, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI87082405, Length=181, Percent_Identity=24.3093922651934, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787574, Length=336, Percent_Identity=22.6190476190476, Blast_Score=69, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6323975, Length=263, Percent_Identity=26.9961977186312, Blast_Score=84, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24581117, Length=232, Percent_Identity=27.1551724137931, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24581115, Length=197, Percent_Identity=26.3959390862944, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000683
- InterPro:   IPR004104 [H]

Pfam domain/function: PF01408 GFO_IDH_MocA; PF02894 GFO_IDH_MocA_C [H]

EC number: =1.1.1.292 [H]

Molecular weight: Translated: 36179; Mature: 36179

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKWGFIGCGEVTKTKSGPAFQKVEHSEVVAVMSRDGAKAKAYAKERGIRKWYDDAQELI
CCEEEEEECCCCCCCCCCCCHHHCCHHHEEEEECCCCCHHHHHHHHCCHHHHHHHHHHHH
DDPEVNAIYIATPPSSHATYAIMAMKAGKPAYIEKPMAVTYEECTRINRISKETGVPCFV
CCCCCCEEEEECCCCCCCEEEEEEEECCCCCCCCCCCEECHHHHHHHHHHHHHCCCCHHH
AYYRRYLPYFQKVKEMVENGSIGNVINVQVRFAQPPRDLDYNRENLPWRVQADIAGGGYF
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCEEE
YDLAPHQIDLLQDMFGCILEASGYKSNRGGLYPAEDTLSACFQFDNGLVGSGSWCFVAHD
EECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCEECCCCEEEEECC
SAREDRIEIIGDKGMICFSVFTYEPIGLHTERGREEICIGNPEHVQQPLIQAVVDHLLGK
CCCCCCEEEECCCCEEEEEEEECCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHCC
SVCSCDGESATLTNWVMDKILGKL
HHHCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MIKWGFIGCGEVTKTKSGPAFQKVEHSEVVAVMSRDGAKAKAYAKERGIRKWYDDAQELI
CCEEEEEECCCCCCCCCCCCHHHCCHHHEEEEECCCCCHHHHHHHHCCHHHHHHHHHHHH
DDPEVNAIYIATPPSSHATYAIMAMKAGKPAYIEKPMAVTYEECTRINRISKETGVPCFV
CCCCCCEEEEECCCCCCCEEEEEEEECCCCCCCCCCCEECHHHHHHHHHHHHHCCCCHHH
AYYRRYLPYFQKVKEMVENGSIGNVINVQVRFAQPPRDLDYNRENLPWRVQADIAGGGYF
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCEEE
YDLAPHQIDLLQDMFGCILEASGYKSNRGGLYPAEDTLSACFQFDNGLVGSGSWCFVAHD
EECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCEECCCCEEEEECC
SAREDRIEIIGDKGMICFSVFTYEPIGLHTERGREEICIGNPEHVQQPLIQAVVDHLLGK
CCCCCCEEEECCCCEEEEEEEECCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHCC
SVCSCDGESATLTNWVMDKILGKL
HHHCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA