Click here to switch to the map view.

The map label for this gene is nuoE [H]

Identifier: 29345533

GI number: 29345533

Start: 112706

End: 113182

Strand: Reverse

Name: nuoE [H]

Synonym: BT_0123

Alternate gene names: 29345533

Gene position: 113182-112706 (Counterclockwise)

Preceding gene: 29345534

Following gene: 29345531

Centisome position: 1.81

GC content: 51.57

Gene sequence:

>477_bases
ATGTCAGATATCAAACTAGCCTGCGATGTGGTGGAACAAGTCAAGACGATTTGCGACAAGCACGGGAACAATGCGGGCGA
ATTAATCAATATTTTGCATGAAGCGCAACATCTGCACGGCTATCTGCCCGAAGAGATGCAACGGATCATAGCCTCCAAAC
TCAGAATCCCCGTGTCGAAAGTCTACGGAGTTGTGACATTCTACACCTTCTTTACGATGACTCCCAAAGGAAAGCATCCG
ATTTCCGTCTGCATGGGCACGGCGTGCTACGTACGTGGTTCGGAGAAGTTGCTGGAAGAGTTCAAGCGGGTACTCGGCAT
CGAAGTCGGAGAAACCACCCCCGACGGAAAGTATTCGCTGGATTGCCTGCGCTGCGTAGGCGCCTGCGGACTGGCTCCGG
TAGTGATGATCGGCGAAAAGGTGTACGGACGACTGCAACCGGTGGATGTAAAGAAGATTATCGAGGAACTGGAATAA

Upstream 100 bases:

>100_bases
TGAAGAATACCTCGGCAAACCGCTGGGTGAGAAATCGGAAATGCTCTTGCACACGCATTATTTCAACAAATCGATTGATT
AACTTTAAACAAGTACTATT

Downstream 100 bases:

>100_bases
GTCTCCCCCGCCCTTCAAAAGAGGATGTGACGTATCGGGTATCCGATATGTCACGCATCTTCTTTGGGGTCATATCCCCA
CTTCACATAAACCGCTCCCC

Product: NADH-ubiquinone oxidoreductase subunit

Products: NA

Alternate protein names: NADH dehydrogenase I subunit E; NDH-1 subunit E [H]

Number of amino acids: Translated: 158; Mature: 157

Protein sequence:

>158_residues
MSDIKLACDVVEQVKTICDKHGNNAGELINILHEAQHLHGYLPEEMQRIIASKLRIPVSKVYGVVTFYTFFTMTPKGKHP
ISVCMGTACYVRGSEKLLEEFKRVLGIEVGETTPDGKYSLDCLRCVGACGLAPVVMIGEKVYGRLQPVDVKKIIEELE

Sequences:

>Translated_158_residues
MSDIKLACDVVEQVKTICDKHGNNAGELINILHEAQHLHGYLPEEMQRIIASKLRIPVSKVYGVVTFYTFFTMTPKGKHP
ISVCMGTACYVRGSEKLLEEFKRVLGIEVGETTPDGKYSLDCLRCVGACGLAPVVMIGEKVYGRLQPVDVKKIIEELE
>Mature_157_residues
SDIKLACDVVEQVKTICDKHGNNAGELINILHEAQHLHGYLPEEMQRIIASKLRIPVSKVYGVVTFYTFFTMTPKGKHPI
SVCMGTACYVRGSEKLLEEFKRVLGIEVGETTPDGKYSLDCLRCVGACGLAPVVMIGEKVYGRLQPVDVKKIIEELE

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG1905

COG function: function code C; NADH:ubiquinone oxidoreductase 24 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 24 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI222080062, Length=137, Percent_Identity=40.8759124087591, Blast_Score=111, Evalue=2e-25,
Organism=Escherichia coli, GI1788621, Length=127, Percent_Identity=33.0708661417323, Blast_Score=79, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17561328, Length=157, Percent_Identity=38.8535031847134, Blast_Score=119, Evalue=9e-28,
Organism=Drosophila melanogaster, GI18859877, Length=138, Percent_Identity=40.5797101449275, Blast_Score=112, Evalue=8e-26,
Organism=Drosophila melanogaster, GI21355353, Length=132, Percent_Identity=34.0909090909091, Blast_Score=93, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002023
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01257 Complex1_24kDa [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 17541; Mature: 17410

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
7.6 %Cys+Met (Translated Protein)
4.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDIKLACDVVEQVKTICDKHGNNAGELINILHEAQHLHGYLPEEMQRIIASKLRIPVSK
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHH
VYGVVTFYTFFTMTPKGKHPISVCMGTACYVRGSEKLLEEFKRVLGIEVGETTPDGKYSL
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCHHH
DCLRCVGACGLAPVVMIGEKVYGRLQPVDVKKIIEELE
HHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHCC
>Mature Secondary Structure 
SDIKLACDVVEQVKTICDKHGNNAGELINILHEAQHLHGYLPEEMQRIIASKLRIPVSK
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHH
VYGVVTFYTFFTMTPKGKHPISVCMGTACYVRGSEKLLEEFKRVLGIEVGETTPDGKYSL
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCHHH
DCLRCVGACGLAPVVMIGEKVYGRLQPVDVKKIIEELE
HHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]