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The map label for this gene is nfnB [H]

Identifier: 291481482

GI number: 291481482

Start: 257472

End: 258134

Strand: Reverse

Name: nfnB [H]

Synonym: NA

Alternate gene names: 291481482

Gene position: 258134-257472 (Counterclockwise)

Preceding gene: 94312650

Following gene: 94312647

Centisome position: 10.0

GC content: 65.01

Gene sequence:

>663_bases
ATGAAAGCCATGAAGCTGTCGGAGATGATCGCCACCCGCCATACCGTGAAGGCGTATGAGCCGGGCCGCGCCATTCCGCA
AGACGTCATCGACGAACTGCTGGCCGTGCTGCACAAGAGCCCGTCATCCGTGAATTCGCAACCGTGGCACTTCGTGGTGG
CGTCCACGCCGGAGGCCCGGGCACGCGTGGCGCGTGCCGCCAGCGGTGGCTACAGCGCCAACGCGCCGAGGATCGAGAAT
GCCTCGCATGTGATCGCGCTGGCCGTGCGCGCCGACATGGACAACGCCCACCTGAACAAGCTGCTGGAAGCCGAGGAGCG
TGATGGCCGCTTTACCGCGGAAGGCGCGAAGGCGGGGCAGGACAAGGGACGTCGATATTTCGTCGACCTGCACCGCTTCG
CGTTTCGCGACGTGCAGCAATGGATGGCGAATCAGGTCTACCTGGCGCTCGGCAGCCTGCTGACGGGCGCCGCGGTACTT
GGCGTGGATGCCACGCCGATGGAAGGCTTCGACCGCAACATCCTCGATGCGGAACTCGGCCTGCGCGAGCGTGGCTATAC
CAGTCTCGTGCTGGTGTCGCTCGGCTACGGCAGCGAGCAGGACTTCAACGCCGACCTCCCCAAGTCGCGGCTCGGCAAGG
AAGAGGTCTTCACGTTCCTGTGA

Upstream 100 bases:
NA

Downstream 100 bases:
NA

Product: dihydropteridine reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:
NA

Sequences:

>Translated_220_residues
MKAMKLSEMIATRHTVKAYEPGRAIPQDVIDELLAVLHKSPSSVNSQPWHFVVASTPEARARVARAASGGYSANAPRIEN
ASHVIALAVRADMDNAHLNKLLEAEERDGRFTAEGAKAGQDKGRRYFVDLHRFAFRDVQQWMANQVYLALGSLLTGAAVL
GVDATPMEGFDRNILDAELGLRERGYTSLVLVSLGYGSEQDFNADLPKSRLGKEEVFTFL
>Mature_220_residues
MKAMKLSEMIATRHTVKAYEPGRAIPQDVIDELLAVLHKSPSSVNSQPWHFVVASTPEARARVARAASGGYSANAPRIEN
ASHVIALAVRADMDNAHLNKLLEAEERDGRFTAEGAKAGQDKGRRYFVDLHRFAFRDVQQWMANQVYLALGSLLTGAAVL
GVDATPMEGFDRNILDAELGLRERGYTSLVLVSLGYGSEQDFNADLPKSRLGKEEVFTFL

Specific function: Reduction of a variety of nitroaromatic compounds using NADH (and to lesser extent NADPH) as source of reducing equivalents; two electrons are transferred. Capable of reducing nitrofurazone [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

Organism=Escherichia coli, GI1786792, Length=215, Percent_Identity=47.4418604651163, Blast_Score=219, Evalue=1e-58,

Paralogues:

None

Copy number: 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.-; 1.5.1.34

Molecular weight: Translated: 24173; Mature: 24173

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAMKLSEMIATRHTVKAYEPGRAIPQDVIDELLAVLHKSPSSVNSQPWHFVVASTPEAR
CCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHH
ARVARAASGGYSANAPRIENASHVIALAVRADMDNAHLNKLLEAEERDGRFTAEGAKAGQ
HHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHCCCCEEECCCCCCC
DKGRRYFVDLHRFAFRDVQQWMANQVYLALGSLLTGAAVLGVDATPMEGFDRNILDAELG
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHC
LRERGYTSLVLVSLGYGSEQDFNADLPKSRLGKEEVFTFL
CCCCCCEEEEEEEECCCCCCCCCCCCCHHHCCHHHHHHCC
>Mature Secondary Structure
MKAMKLSEMIATRHTVKAYEPGRAIPQDVIDELLAVLHKSPSSVNSQPWHFVVASTPEAR
CCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHH
ARVARAASGGYSANAPRIENASHVIALAVRADMDNAHLNKLLEAEERDGRFTAEGAKAGQ
HHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHCCCCEEECCCCCCC
DKGRRYFVDLHRFAFRDVQQWMANQVYLALGSLLTGAAVLGVDATPMEGFDRNILDAELG
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHC
LRERGYTSLVLVSLGYGSEQDFNADLPKSRLGKEEVFTFL
CCCCCCEEEEEEEECCCCCCCCCCCCCHHHCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2179862; 11677609 [H]