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The map label for this gene is yiaE [H]

Identifier: 29144149

GI number: 29144149

Start: 3999261

End: 4000235

Strand: Reverse

Name: yiaE [H]

Synonym: t3873

Alternate gene names: 29144149

Gene position: 4000235-3999261 (Counterclockwise)

Preceding gene: 29144150

Following gene: 29144147

Centisome position: 83.48

GC content: 57.03

Gene sequence:

>975_bases
ATGAAGCCGTCCATCATTCTCTACAAAACCCTGCCTGATGATTTGCTGCATCGCCTGGAGGCGCATTTTACCGTCACCCA
GGTACCGAACCTGCATCCGGAAACGGTCGCGCGACACGCGCAGGCATTTGCCAGCGCGCAAGGCCTTCTGGGCACCAGCG
AAACCGTTAACCGCGCGCTGCTGGAAAAAATGCCGGCGCTACGCGCGGCGTCGACCATCTCGGTGGGATACGATAATGTC
GAGGTGGACGCCCTTACTGCCCGCAAAATAGTCCTGATGCACACGCCTGCCGTTTTGACGGAAACCGTTGCCGATACGGT
GATGGCGTTGATGCTGGCCACTGCCCGCCGCGTGGTGGACGTCGCAGAACGGGTTAAGGCGGGTGAATGGACAGAAAGCA
TTGGCCCGGCATGGTTTGGCGTCGATGTGCATCACAAAACACTCGGTATCGTTGGGATGGGGCGTATCGGGATGGCGCTG
GCGCAGCGAGCGCACTTTGGGTTTACCATGCCGGTTCTCTATCACGCCCGTCGGCGGCATCAGGAGGCGGAAGACCGGTT
TAACGCCCGCTACTGCGATCTGGATACGTTGTTGCAGGAGGCCGATTTTGTCTGCGTGATCCTGCCACTAACGGCAGAAA
CGCGACATCTGTTTGGCGCAACACAGTTTGCCAGAATGAAATCGTCCGCCATTTTTATTAATGCCGGACGCGGCCCGGTG
GTCGATGAAAACGCGCTGATTGCCGCGCTGCAAAACGGTGAAATATATGCGGCAGGTCTGGATGTCTTTGAACAGGAACC
GCTATCTGTGGATTCGCCCCTGCTGAACATGTCTAATGTCGTCGCGGTGCCGCATATTGGCTCGGCAACGCATGAGACGC
GCTACAACATGATGGCCTGTGCGGTGGATAATTTGATTGATGCGCTGCAGGGAAAAATAGAGAAGAACTGCGTCAATCCG
CAAGCGGCGGGATAA

Upstream 100 bases:

>100_bases
AGCGGGGAAATCGGCTGGTCGATGATGGCTAAACGGGTTAATAATAAGCCTGTTCCGGTTGGCGCATGCCGCGCTTACCT
TTTGATTTACGGAGAGTCGC

Downstream 100 bases:

>100_bases
CGCTACAGGCCCGATAAGCGCTGGCTTGCGCTTATCGGGCAGACTGTCATTACTGGGTCGCGTTTACCGCCGCCGTCCAG
GCCTGATTAAACGCCTGATG

Product: 2-hydroxyacid dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MKPSIILYKTLPDDLLHRLEAHFTVTQVPNLHPETVARHAQAFASAQGLLGTSETVNRALLEKMPALRAASTISVGYDNV
EVDALTARKIVLMHTPAVLTETVADTVMALMLATARRVVDVAERVKAGEWTESIGPAWFGVDVHHKTLGIVGMGRIGMAL
AQRAHFGFTMPVLYHARRRHQEAEDRFNARYCDLDTLLQEADFVCVILPLTAETRHLFGATQFARMKSSAIFINAGRGPV
VDENALIAALQNGEIYAAGLDVFEQEPLSVDSPLLNMSNVVAVPHIGSATHETRYNMMACAVDNLIDALQGKIEKNCVNP
QAAG

Sequences:

>Translated_324_residues
MKPSIILYKTLPDDLLHRLEAHFTVTQVPNLHPETVARHAQAFASAQGLLGTSETVNRALLEKMPALRAASTISVGYDNV
EVDALTARKIVLMHTPAVLTETVADTVMALMLATARRVVDVAERVKAGEWTESIGPAWFGVDVHHKTLGIVGMGRIGMAL
AQRAHFGFTMPVLYHARRRHQEAEDRFNARYCDLDTLLQEADFVCVILPLTAETRHLFGATQFARMKSSAIFINAGRGPV
VDENALIAALQNGEIYAAGLDVFEQEPLSVDSPLLNMSNVVAVPHIGSATHETRYNMMACAVDNLIDALQGKIEKNCVNP
QAAG
>Mature_324_residues
MKPSIILYKTLPDDLLHRLEAHFTVTQVPNLHPETVARHAQAFASAQGLLGTSETVNRALLEKMPALRAASTISVGYDNV
EVDALTARKIVLMHTPAVLTETVADTVMALMLATARRVVDVAERVKAGEWTESIGPAWFGVDVHHKTLGIVGMGRIGMAL
AQRAHFGFTMPVLYHARRRHQEAEDRFNARYCDLDTLLQEADFVCVILPLTAETRHLFGATQFARMKSSAIFINAGRGPV
VDENALIAALQNGEIYAAGLDVFEQEPLSVDSPLLNMSNVVAVPHIGSATHETRYNMMACAVDNLIDALQGKIEKNCVNP
QAAG

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrB subfamily [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=295, Percent_Identity=39.3220338983051, Blast_Score=190, Evalue=1e-48,
Organism=Homo sapiens, GI23308577, Length=271, Percent_Identity=31.7343173431734, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI145580578, Length=276, Percent_Identity=31.5217391304348, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI4557499, Length=276, Percent_Identity=31.5217391304348, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI145580575, Length=274, Percent_Identity=31.7518248175182, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI4557497, Length=275, Percent_Identity=32, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI61743967, Length=275, Percent_Identity=32, Blast_Score=122, Evalue=4e-28,
Organism=Escherichia coli, GI87082289, Length=323, Percent_Identity=82.9721362229102, Blast_Score=565, Evalue=1e-162,
Organism=Escherichia coli, GI1787645, Length=204, Percent_Identity=31.8627450980392, Blast_Score=101, Evalue=8e-23,
Organism=Escherichia coli, GI1789279, Length=254, Percent_Identity=30.7086614173228, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI87081824, Length=156, Percent_Identity=30.1282051282051, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17532191, Length=249, Percent_Identity=30.9236947791165, Blast_Score=127, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI25147481, Length=275, Percent_Identity=28.3636363636364, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6324055, Length=244, Percent_Identity=37.7049180327869, Blast_Score=155, Evalue=6e-39,
Organism=Saccharomyces cerevisiae, GI6320925, Length=242, Percent_Identity=33.0578512396694, Blast_Score=119, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6322116, Length=245, Percent_Identity=31.0204081632653, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6325144, Length=197, Percent_Identity=32.48730964467, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324964, Length=248, Percent_Identity=27.8225806451613, Blast_Score=93, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6321253, Length=168, Percent_Identity=27.9761904761905, Blast_Score=70, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6324980, Length=170, Percent_Identity=28.2352941176471, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI45551003, Length=313, Percent_Identity=38.9776357827476, Blast_Score=202, Evalue=3e-52,
Organism=Drosophila melanogaster, GI24585514, Length=313, Percent_Identity=38.9776357827476, Blast_Score=202, Evalue=3e-52,
Organism=Drosophila melanogaster, GI28574282, Length=313, Percent_Identity=38.9776357827476, Blast_Score=202, Evalue=3e-52,
Organism=Drosophila melanogaster, GI28574284, Length=313, Percent_Identity=38.9776357827476, Blast_Score=202, Evalue=3e-52,
Organism=Drosophila melanogaster, GI45552429, Length=313, Percent_Identity=38.9776357827476, Blast_Score=202, Evalue=3e-52,
Organism=Drosophila melanogaster, GI28574286, Length=312, Percent_Identity=37.1794871794872, Blast_Score=199, Evalue=2e-51,
Organism=Drosophila melanogaster, GI28571528, Length=311, Percent_Identity=37.2990353697749, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24585516, Length=248, Percent_Identity=39.1129032258064, Blast_Score=173, Evalue=2e-43,
Organism=Drosophila melanogaster, GI62472511, Length=270, Percent_Identity=32.962962962963, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24646446, Length=270, Percent_Identity=32.962962962963, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24646448, Length=270, Percent_Identity=32.962962962963, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24646452, Length=270, Percent_Identity=32.962962962963, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24646450, Length=270, Percent_Identity=32.962962962963, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI19921140, Length=248, Percent_Identity=29.0322580645161, Blast_Score=114, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.79; =1.1.1.81 [H]

Molecular weight: Translated: 35313; Mature: 35313

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPSIILYKTLPDDLLHRLEAHFTVTQVPNLHPETVARHAQAFASAQGLLGTSETVNRAL
CCCCEEEEECCCHHHHHHHHHCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHH
LEKMPALRAASTISVGYDNVEVDALTARKIVLMHTPAVLTETVADTVMALMLATARRVVD
HHHCCHHHHHCEEEECCCCEEEEEEECCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
VAERVKAGEWTESIGPAWFGVDVHHKTLGIVGMGRIGMALAQRAHFGFTMPVLYHARRRH
HHHHHHCCCCHHHCCCEEEEEEECCCEEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHH
QEAEDRFNARYCDLDTLLQEADFVCVILPLTAETRHLFGATQFARMKSSAIFINAGRGPV
HHHHHHHCCEEECHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHCCCEEEEECCCCCC
VDENALIAALQNGEIYAAGLDVFEQEPLSVDSPLLNMSNVVAVPHIGSATHETRYNMMAC
CCCCCEEEEEECCEEEEECCCHHCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHH
AVDNLIDALQGKIEKNCVNPQAAG
HHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKPSIILYKTLPDDLLHRLEAHFTVTQVPNLHPETVARHAQAFASAQGLLGTSETVNRAL
CCCCEEEEECCCHHHHHHHHHCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHH
LEKMPALRAASTISVGYDNVEVDALTARKIVLMHTPAVLTETVADTVMALMLATARRVVD
HHHCCHHHHHCEEEECCCCEEEEEEECCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
VAERVKAGEWTESIGPAWFGVDVHHKTLGIVGMGRIGMALAQRAHFGFTMPVLYHARRRH
HHHHHHCCCCHHHCCCEEEEEEECCCEEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHH
QEAEDRFNARYCDLDTLLQEADFVCVILPLTAETRHLFGATQFARMKSSAIFINAGRGPV
HHHHHHHCCEEECHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHCCCEEEEECCCCCC
VDENALIAALQNGEIYAAGLDVFEQEPLSVDSPLLNMSNVVAVPHIGSATHETRYNMMAC
CCCCCEEEEEECCEEEEECCCHHCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHH
AVDNLIDALQGKIEKNCVNPQAAG
HHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA