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The map label for this gene is gmuD [H]

Identifier: 29144032

GI number: 29144032

Start: 3856960

End: 3858342

Strand: Reverse

Name: gmuD [H]

Synonym: t3743

Alternate gene names: 29144032

Gene position: 3858342-3856960 (Counterclockwise)

Preceding gene: 29144033

Following gene: 29144030

Centisome position: 80.52

GC content: 53.8

Gene sequence:

>1383_bases
ATGCGATACCGTTTTCCCGATAACTTCTGGTGGGGCAGCGCCTGCTCAGCGTTGCAAACCGAAGGGGATAGTCTGAATGG
TGGTAAAAGCCAGACCACGTGGGATGTGTGGTTCGAGCGCCAGCCTGATCGTTTTCATCAGGGCATCGGTCCAGCGGAAA
CCTCAACGTTCTATCGCCACTGGAAGCAAGACATCGCGCTACTGAAACAGTTAAAACACAACAGTTTTCGCACCTCGCTA
AGCTGGGCGCGGCTCATTCCAGACGGTGTAGGCGAGGTGAATCCACAAGCGGTGAGCTTCTACAATCACGTCATCGACGA
GCTACTGGCGCAGGGCATCACGCCGTTTATTACGCTGTTCCATTTTGATATGCCGATGGTCATGCAGGAGAAAGGCGGCT
GGGAAAATCGCGACGTCGTAGAGGCGTTTGGTCGGTACGCGCAAACGTGTTTTACCTTGTTTGGCGACCGCGTGAAGCAC
TGGTTTACCTTTAACGAGCCGATTGTGCCGGTGGAAGGCGGCTATTTGTACGACTTCCACTATCCCAATGTGGTGGATTT
TAAACGTGCGGCCACCGTGGCGTACCATACCGTGCTGGCGCACTCGACCGCCGTGCGCGCCTGGCGCGCCGGGCGCTACG
ACGGTGAAATCGGCGTAGTGCTGAATCTGACGCCGTCCTACCCACGCTCGCAGCATCCCGCCGATGTGCAAGCCGCGCAT
CATGCGGATCTGTTATTCAACCGCAGTTTTCTTGACCCGGTATTAAAGGGAGAATACCCGGCGGAGCTGGTGGCGCTGCT
GAAAACCTATGACCAGCTGCCTGCCTGTCAGCCAGGCGACCGTCAGCTTATTGCCGACGGCAAAATCGATTTACTGGGGA
TTAACTATTATCAGCCGCGCCGCGTGAAATGCCGTGATACAGCGGTGAATCCGCAAGCGCCGTTTATGCCGGAGTGGTTA
TTTGACTATTACGACATGCCGGGGCGCAAGATGAACCCTTACCGCGGCTGGGAAATTTACGCGCCAGGAATTTACGACAT
CATCACCAACCTGCGGGATAATTACGGCAATCCGCGCTGTTTTATCTCCGAAAACGGGATGGGCGTTGAGAACGAGCAGC
GTTTTGTGCAAGCGGGACAGATTCACGATGATTACCGGATTGACTTTATCTCTGAGCATCTTAAATGGCTGCATAAAGGC
ATTAGCGAGGGCTGTCACTGTCTTGGCTACCACATGTGGACCTTTATCGATAACTGGTCATGGCTGAACGGCTATAAAAA
TCGCTATGGTTTTGTACAACTGGATTTAGCCACCCAAACGCGCACGGTGAAAAAAAGCGGAGAATGGTTTGCCGCCACCG
CAGAGCATAACGGTTTTGATTAA

Upstream 100 bases:

>100_bases
CTTTTCCGCTTTTTGGTCGCGAAAATGCGGACTGTAACCGGTTTCTGTTCGCCAAAAGCGCATATTCTGGAGCGAATTTA
CGCGATCAAGGAGTACGACA

Downstream 100 bases:

>100_bases
GCGTTTATTATTGCCGGATAACGACTAACGTCGTATCCGGCCTACAAATGGCAGCAATATCAACTCATTGGCGTAATACG
GACTTTCGCCGCCACGACTA

Product: glycosyl hydrolase

Products: NA

Alternate protein names: Aryl-phospho-beta-D-glucosidase BglD; Glucomannan utilization protein D [H]

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MRYRFPDNFWWGSACSALQTEGDSLNGGKSQTTWDVWFERQPDRFHQGIGPAETSTFYRHWKQDIALLKQLKHNSFRTSL
SWARLIPDGVGEVNPQAVSFYNHVIDELLAQGITPFITLFHFDMPMVMQEKGGWENRDVVEAFGRYAQTCFTLFGDRVKH
WFTFNEPIVPVEGGYLYDFHYPNVVDFKRAATVAYHTVLAHSTAVRAWRAGRYDGEIGVVLNLTPSYPRSQHPADVQAAH
HADLLFNRSFLDPVLKGEYPAELVALLKTYDQLPACQPGDRQLIADGKIDLLGINYYQPRRVKCRDTAVNPQAPFMPEWL
FDYYDMPGRKMNPYRGWEIYAPGIYDIITNLRDNYGNPRCFISENGMGVENEQRFVQAGQIHDDYRIDFISEHLKWLHKG
ISEGCHCLGYHMWTFIDNWSWLNGYKNRYGFVQLDLATQTRTVKKSGEWFAATAEHNGFD

Sequences:

>Translated_460_residues
MRYRFPDNFWWGSACSALQTEGDSLNGGKSQTTWDVWFERQPDRFHQGIGPAETSTFYRHWKQDIALLKQLKHNSFRTSL
SWARLIPDGVGEVNPQAVSFYNHVIDELLAQGITPFITLFHFDMPMVMQEKGGWENRDVVEAFGRYAQTCFTLFGDRVKH
WFTFNEPIVPVEGGYLYDFHYPNVVDFKRAATVAYHTVLAHSTAVRAWRAGRYDGEIGVVLNLTPSYPRSQHPADVQAAH
HADLLFNRSFLDPVLKGEYPAELVALLKTYDQLPACQPGDRQLIADGKIDLLGINYYQPRRVKCRDTAVNPQAPFMPEWL
FDYYDMPGRKMNPYRGWEIYAPGIYDIITNLRDNYGNPRCFISENGMGVENEQRFVQAGQIHDDYRIDFISEHLKWLHKG
ISEGCHCLGYHMWTFIDNWSWLNGYKNRYGFVQLDLATQTRTVKKSGEWFAATAEHNGFD
>Mature_460_residues
MRYRFPDNFWWGSACSALQTEGDSLNGGKSQTTWDVWFERQPDRFHQGIGPAETSTFYRHWKQDIALLKQLKHNSFRTSL
SWARLIPDGVGEVNPQAVSFYNHVIDELLAQGITPFITLFHFDMPMVMQEKGGWENRDVVEAFGRYAQTCFTLFGDRVKH
WFTFNEPIVPVEGGYLYDFHYPNVVDFKRAATVAYHTVLAHSTAVRAWRAGRYDGEIGVVLNLTPSYPRSQHPADVQAAH
HADLLFNRSFLDPVLKGEYPAELVALLKTYDQLPACQPGDRQLIADGKIDLLGINYYQPRRVKCRDTAVNPQAPFMPEWL
FDYYDMPGRKMNPYRGWEIYAPGIYDIITNLRDNYGNPRCFISENGMGVENEQRFVQAGQIHDDYRIDFISEHLKWLHKG
ISEGCHCLGYHMWTFIDNWSWLNGYKNRYGFVQLDLATQTRTVKKSGEWFAATAEHNGFD

Specific function: Phospho-beta-D-glucosidase that seems to be involved in the degradation of glucomannan. Is also capable of hydrolyzing aryl-phospho-beta-D-glucosides, although very weakly, and plays only a minor role, if any, in the degradation of these substrates in viv

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Homo sapiens, GI110681710, Length=491, Percent_Identity=30.3462321792261, Blast_Score=203, Evalue=2e-52,
Organism=Homo sapiens, GI13273313, Length=482, Percent_Identity=28.2157676348548, Blast_Score=202, Evalue=5e-52,
Organism=Homo sapiens, GI32481206, Length=489, Percent_Identity=31.4928425357873, Blast_Score=192, Evalue=4e-49,
Organism=Homo sapiens, GI28376633, Length=466, Percent_Identity=25.1072961373391, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI24497614, Length=480, Percent_Identity=25.625, Blast_Score=137, Evalue=3e-32,
Organism=Escherichia coli, GI1789070, Length=490, Percent_Identity=33.6734693877551, Blast_Score=228, Evalue=6e-61,
Organism=Escherichia coli, GI2367174, Length=491, Percent_Identity=31.1608961303462, Blast_Score=210, Evalue=2e-55,
Organism=Escherichia coli, GI2367270, Length=476, Percent_Identity=29.8319327731092, Blast_Score=179, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI17539390, Length=467, Percent_Identity=31.9057815845824, Blast_Score=229, Evalue=3e-60,
Organism=Caenorhabditis elegans, GI17552856, Length=470, Percent_Identity=30.6382978723404, Blast_Score=217, Evalue=1e-56,
Organism=Drosophila melanogaster, GI21356577, Length=481, Percent_Identity=27.4428274428274, Blast_Score=197, Evalue=2e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 53245; Mature: 53245

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS00572 GLYCOSYL_HYDROL_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYRFPDNFWWGSACSALQTEGDSLNGGKSQTTWDVWFERQPDRFHQGIGPAETSTFYRH
CCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCEEEEEECCCCHHHHCCCCCCHHHHHHHH
WKQDIALLKQLKHNSFRTSLSWARLIPDGVGEVNPQAVSFYNHVIDELLAQGITPFITLF
HHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHH
HFDMPMVMQEKGGWENRDVVEAFGRYAQTCFTLFGDRVKHWFTFNEPIVPVEGGYLYDFH
HCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEECCCCEEEEEC
YPNVVDFKRAATVAYHTVLAHSTAVRAWRAGRYDGEIGVVLNLTPSYPRSQHPADVQAAH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCHHHH
HADLLFNRSFLDPVLKGEYPAELVALLKTYDQLPACQPGDRQLIADGKIDLLGINYYQPR
HHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEEEECCCCCC
RVKCRDTAVNPQAPFMPEWLFDYYDMPGRKMNPYRGWEIYAPGIYDIITNLRDNYGNPRC
CCEEECCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCEEECCHHHHHHHHHHHCCCCCEE
FISENGMGVENEQRFVQAGQIHDDYRIDFISEHLKWLHKGISEGCHCLGYHMWTFIDNWS
EEECCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
WLNGYKNRYGFVQLDLATQTRTVKKSGEWFAATAEHNGFD
HHCCCCCCCCEEEEEECHHHHHHHCCCCEEEEECCCCCCC
>Mature Secondary Structure
MRYRFPDNFWWGSACSALQTEGDSLNGGKSQTTWDVWFERQPDRFHQGIGPAETSTFYRH
CCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCEEEEEECCCCHHHHCCCCCCHHHHHHHH
WKQDIALLKQLKHNSFRTSLSWARLIPDGVGEVNPQAVSFYNHVIDELLAQGITPFITLF
HHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHH
HFDMPMVMQEKGGWENRDVVEAFGRYAQTCFTLFGDRVKHWFTFNEPIVPVEGGYLYDFH
HCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEECCCCEEEEEC
YPNVVDFKRAATVAYHTVLAHSTAVRAWRAGRYDGEIGVVLNLTPSYPRSQHPADVQAAH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCHHHH
HADLLFNRSFLDPVLKGEYPAELVALLKTYDQLPACQPGDRQLIADGKIDLLGINYYQPR
HHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEEEECCCCCC
RVKCRDTAVNPQAPFMPEWLFDYYDMPGRKMNPYRGWEIYAPGIYDIITNLRDNYGNPRC
CCEEECCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCEEECCHHHHHHHHHHHCCCCCEE
FISENGMGVENEQRFVQAGQIHDDYRIDFISEHLKWLHKGISEGCHCLGYHMWTFIDNWS
EEECCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
WLNGYKNRYGFVQLDLATQTRTVKKSGEWFAATAEHNGFD
HHCCCCCCCCEEEEEECHHHHHHHCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9202461; 9384377 [H]