Click here to switch to the map view.

The map label for this gene is dsbA [H]

Identifier: 29143922

GI number: 29143922

Start: 3726168

End: 3726791

Strand: Reverse

Name: dsbA [H]

Synonym: t3623

Alternate gene names: 29143922

Gene position: 3726791-3726168 (Counterclockwise)

Preceding gene: 29143923

Following gene: 29143920

Centisome position: 77.77

GC content: 50.16

Gene sequence:

>624_bases
ATGAAAAAGATTTGGCTGGCGCTGGCTGGTATGGTTTTAGCTTTTAGCGCCTCGGCAGCACAGATCAGCGACGGTAAACA
GTATATCACGCTGGATAAACCGGTCGCTGGCGAACCCCAGGTACTGGAGTTTTTCTCATTCTACTGCCCACATTGTTATC
AGTTTGAAGAAGTGCTTCATGTGTCTGACAATGTGAAGAAAAAGCTGCCGAAAGGCACCAAGATGACCAAGTACCACGTT
GAGTTCCTGGGGCCGTTGGGCAAGGAGCTCACCCAGGCATGGGCGGTGGCGATGGCGTTGGGGGTAGAAGATAAAGTCAC
GGTCCCGCTGTTTGAAGCCGTACAGAAAACCCAGACAGTACAATCTGCCGCGGATATCCGTAAAGTGTTCGTTGATGCGG
GCGTAAAAGGCGAAGATTACGATGCGGCATGGAACAGCTTCGTGGTGAAATCACTGGTTGCGCAACAGGAGAAAGCCGCG
GCTGACCTGCAACTGCAGGGCGTTCCGGCGATGTTCGTCAATGGCAAATACCAGATTAACCCACAAGGCATGGATACCAG
CAGCATGGATGTTTTTGTTCAGCAGTATGCTGATACCGTGAAATATTTGGTTGATAAAAAATAA

Upstream 100 bases:

>100_bases
TACTGGCAGCGACAGACAACGACTTTTATTGAGCAGACTAAAATTTTGCACGAACCCCCTTTACAATTAACGCCAATGTA
TTAATCGGAGAGAGTTGATC

Downstream 100 bases:

>100_bases
AAAGAACGCCGGTCACTGACCGGCGTTTTTATAAGATGTTTTAATCTCTTCTATCTGTATATCTTTCTTATCCCACAGCG
TATTAAGCCAACGTTGAAAA

Product: periplasmic protein disulfide isomerase I

Products: 3-oxo-D 4-steroid; protein with oxidized disulfide bonds [C]

Alternate protein names: NA

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MKKIWLALAGMVLAFSASAAQISDGKQYITLDKPVAGEPQVLEFFSFYCPHCYQFEEVLHVSDNVKKKLPKGTKMTKYHV
EFLGPLGKELTQAWAVAMALGVEDKVTVPLFEAVQKTQTVQSAADIRKVFVDAGVKGEDYDAAWNSFVVKSLVAQQEKAA
ADLQLQGVPAMFVNGKYQINPQGMDTSSMDVFVQQYADTVKYLVDKK

Sequences:

>Translated_207_residues
MKKIWLALAGMVLAFSASAAQISDGKQYITLDKPVAGEPQVLEFFSFYCPHCYQFEEVLHVSDNVKKKLPKGTKMTKYHV
EFLGPLGKELTQAWAVAMALGVEDKVTVPLFEAVQKTQTVQSAADIRKVFVDAGVKGEDYDAAWNSFVVKSLVAQQEKAA
ADLQLQGVPAMFVNGKYQINPQGMDTSSMDVFVQQYADTVKYLVDKK
>Mature_207_residues
MKKIWLALAGMVLAFSASAAQISDGKQYITLDKPVAGEPQVLEFFSFYCPHCYQFEEVLHVSDNVKKKLPKGTKMTKYHV
EFLGPLGKELTQAWAVAMALGVEDKVTVPLFEAVQKTQTVQSAADIRKVFVDAGVKGEDYDAAWNSFVVKSLVAQQEKAA
ADLQLQGVPAMFVNGKYQINPQGMDTSSMDVFVQQYADTVKYLVDKK

Specific function: Required for disulfide bond formation in some periplasmic proteins such as PhoA or OmpA. Acts by transferring its disulfide bond to other proteins and is reduced in the process. DsbA is reoxidized by DsbB. It is required for pilus biogenesis [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

Organism=Escherichia coli, GI1790291, Length=208, Percent_Identity=85.5769230769231, Blast_Score=374, Evalue=1e-105,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1348 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001853
- InterPro:   IPR023205
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01323 DSBA [H]

EC number: NA

Molecular weight: Translated: 22911; Mature: 22911

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS00194 THIOREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIWLALAGMVLAFSASAAQISDGKQYITLDKPVAGEPQVLEFFSFYCPHCYQFEEVLH
CHHHHHHHHHHHHHHCCCHHCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHH
VSDNVKKKLPKGTKMTKYHVEFLGPLGKELTQAWAVAMALGVEDKVTVPLFEAVQKTQTV
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
QSAADIRKVFVDAGVKGEDYDAAWNSFVVKSLVAQQEKAAADLQLQGVPAMFVNGKYQIN
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCEEECCCCEEEECCEEEEC
PQGMDTSSMDVFVQQYADTVKYLVDKK
CCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKIWLALAGMVLAFSASAAQISDGKQYITLDKPVAGEPQVLEFFSFYCPHCYQFEEVLH
CHHHHHHHHHHHHHHCCCHHCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHH
VSDNVKKKLPKGTKMTKYHVEFLGPLGKELTQAWAVAMALGVEDKVTVPLFEAVQKTQTV
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
QSAADIRKVFVDAGVKGEDYDAAWNSFVVKSLVAQQEKAAADLQLQGVPAMFVNGKYQIN
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCEEECCCCEEEECCEEEEC
PQGMDTSSMDVFVQQYADTVKYLVDKK
CCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxo-D 5-steroid; protein with reduced SH groups [C]

Specific reaction: 3-oxo-D 5-steroid = 3-oxo-D 4-steroid protein with reduced SH groups = protein with oxidized disulfide bonds [C]

General reaction: Isomerases; Intramolecular Oxidoreductases; Transposing S-S Bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA