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The map label for this gene is btuB [H]

Identifier: 29143801

GI number: 29143801

Start: 3586907

End: 3588751

Strand: Reverse

Name: btuB [H]

Synonym: t3495

Alternate gene names: 29143801

Gene position: 3588751-3586907 (Counterclockwise)

Preceding gene: 29143803

Following gene: 161486778

Centisome position: 74.89

GC content: 51.71

Gene sequence:

>1845_bases
ATGATTAAAAAAGCTACGCTGCTGACGGCGTTCTCCGTCACGGCCTTTTCAGCTTGGGCGCAGGACACTAGCCCGGATAC
ACTGGTTGTCACCGCCAACCGTTTTCAGCAGCCGCGCAGCGCGGTTCTGGCGCCCGTTACCATCGTGACGCGTCAGGATA
TTGAACGCTGGCAATCGACCTCCGTAAATGATGTTCTACGCCGTTTGCCTGGCGTCGATATTGCGCAGAGCGGCGGCACC
GGACAAAACTCCTCCATTTTCATTCGCGGCACCAACTCCAGCCATGTACTGGTATTGATTGACGGCGTGCGTCTGAATTT
AGCAGGCGTGAGCGGGTCCGCCGATCTCAGCCAGTTCCCGGTGTCGCTGGTACAGCGCATTGAATATATTCGCGGTCCGC
GTTCCGCTATTTATGGTTCCGATGCTATCGGCGGCGTAGTGAATATCATTACGACGCGCGATAACCCAGGCACAGAATTA
ACCGCTGGATGGGGAAGCAATAGCTACCAGAATTACGACCTCTCGACGCAACAGCAACTTGGCGAAAACACGCGGGCGAC
GTTGATCGGCGATTACGAATACACCAAAGGGTTTGACGTGGTAGCGAAAGGCGGTACCGGGATGCAGGCGCAGCCTGACC
GGGACGGCTTTTTGAGTAAAACGCTTTATGGCGCGTTAGAGCATACCTTTTCTGATCGCTGGAGCGGATTCGTGCGTGGT
TATGGCTACGATAACCGTACCGATTACGACGCCTATTACTCGCCGGGCTCGCCGCTGATTGATACACGCAAACTTTATAG
CCAAAGCTGGGACGCCGGGCTGCGCTTTAATGGCGAACGTATTCAGTCTCAGCTAGTTTCAAGCTATAGCCACAGTAAAG
ATTACAACTATGATCCGCACTATGGTCGGTATGATACCTCCGCCACGCTGGATGAGATGAAACAGTACAATGTTCAATGG
ACCAACAGTGTGGTTGTGGGGCACGGTAATGTTGGGGCGGGCGTAGACTGGCAGAAACAGACTACCACGCCAGGTACCGG
CTATGTGCCCGAGGGATATGACCAGCGTAATACCGGGGTTTACCTGACAGGATTACAACAGTTGGGTGACTTCACCCTGG
AAGCGGCGGCGCGCAGTGATGACAACTCCCAGTTTGGTCGTCACGGTACATGGCAAACCAGCGCGGGATGGGAGTTTATA
GAAGGTTATCGCTTTATTGCCTCCTACGGAACCTCCTACAAAGCGCCTAATTTGGGCCAGCTGTATGGGTATTACGGTAA
TCCGAACCTGAATCCTGAAAAGAGTAAACAGTGGGAAGGCGCATTTGAAGGGCTCACCGCTGGCGTCAGCTGGCGTATTT
CAGGTTATCGTAACGATATTAATGACATGATCGATTATGACGATCATCTGCAAAAATATTACAACGAAGGTAAGGCGCGT
ATTAAAGGTATTGAGGCGACGGCGAATTTCGATACCGGACCGTTAACGCATACGGTCAGTTATGATTACGTTGATGCGCG
TAATGCGATTACCGATACGCCATTACCCCGGCGTTCCAAACAGATGGCAAAATATCAACTTGACTGGGACGTTTACGATT
TTGACTGGGGGGTGACATATCAATACCTTGGTTCCCGCTATGATTCGGATTACTCCGCTTACCCATACCGGACAGTAAAA
ATGGGCGGCGTCAGTTTATGGGATCTTACGGTTGCATATCCGGTCACCTCACATCTGACAGTTCGTGGTAAAATAGCCAA
CCTGTTCGACAAAGATTACGAGACAGTTTATGGCTACCAAACTGCAGGACGAGAATACACCTTGTCTGGCAGCTACACCT
TCTGA

Upstream 100 bases:

>100_bases
AAGCCCGAAGACCTGCCGGCTAACGTCGCATCTGGTTTTTCATCATCGCGTACTATCGATGAAGCCTGCGGCATCCTTCT
TATATTGTGGATGCTTTACA

Downstream 100 bases:

>100_bases
ACCACGTCCCACCGTGCTGGTATTTGATTCCGGCGTCGGTGGATTGTCGGTCTATGATGAGATTCGGCGGCTCCTGCCGG
ATCTCCACTATATATATGCT

Product: vitamin B12/cobalamin outer membrane transporter

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 614; Mature: 614

Protein sequence:

>614_residues
MIKKATLLTAFSVTAFSAWAQDTSPDTLVVTANRFQQPRSAVLAPVTIVTRQDIERWQSTSVNDVLRRLPGVDIAQSGGT
GQNSSIFIRGTNSSHVLVLIDGVRLNLAGVSGSADLSQFPVSLVQRIEYIRGPRSAIYGSDAIGGVVNIITTRDNPGTEL
TAGWGSNSYQNYDLSTQQQLGENTRATLIGDYEYTKGFDVVAKGGTGMQAQPDRDGFLSKTLYGALEHTFSDRWSGFVRG
YGYDNRTDYDAYYSPGSPLIDTRKLYSQSWDAGLRFNGERIQSQLVSSYSHSKDYNYDPHYGRYDTSATLDEMKQYNVQW
TNSVVVGHGNVGAGVDWQKQTTTPGTGYVPEGYDQRNTGVYLTGLQQLGDFTLEAAARSDDNSQFGRHGTWQTSAGWEFI
EGYRFIASYGTSYKAPNLGQLYGYYGNPNLNPEKSKQWEGAFEGLTAGVSWRISGYRNDINDMIDYDDHLQKYYNEGKAR
IKGIEATANFDTGPLTHTVSYDYVDARNAITDTPLPRRSKQMAKYQLDWDVYDFDWGVTYQYLGSRYDSDYSAYPYRTVK
MGGVSLWDLTVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF

Sequences:

>Translated_614_residues
MIKKATLLTAFSVTAFSAWAQDTSPDTLVVTANRFQQPRSAVLAPVTIVTRQDIERWQSTSVNDVLRRLPGVDIAQSGGT
GQNSSIFIRGTNSSHVLVLIDGVRLNLAGVSGSADLSQFPVSLVQRIEYIRGPRSAIYGSDAIGGVVNIITTRDNPGTEL
TAGWGSNSYQNYDLSTQQQLGENTRATLIGDYEYTKGFDVVAKGGTGMQAQPDRDGFLSKTLYGALEHTFSDRWSGFVRG
YGYDNRTDYDAYYSPGSPLIDTRKLYSQSWDAGLRFNGERIQSQLVSSYSHSKDYNYDPHYGRYDTSATLDEMKQYNVQW
TNSVVVGHGNVGAGVDWQKQTTTPGTGYVPEGYDQRNTGVYLTGLQQLGDFTLEAAARSDDNSQFGRHGTWQTSAGWEFI
EGYRFIASYGTSYKAPNLGQLYGYYGNPNLNPEKSKQWEGAFEGLTAGVSWRISGYRNDINDMIDYDDHLQKYYNEGKAR
IKGIEATANFDTGPLTHTVSYDYVDARNAITDTPLPRRSKQMAKYQLDWDVYDFDWGVTYQYLGSRYDSDYSAYPYRTVK
MGGVSLWDLTVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF
>Mature_614_residues
MIKKATLLTAFSVTAFSAWAQDTSPDTLVVTANRFQQPRSAVLAPVTIVTRQDIERWQSTSVNDVLRRLPGVDIAQSGGT
GQNSSIFIRGTNSSHVLVLIDGVRLNLAGVSGSADLSQFPVSLVQRIEYIRGPRSAIYGSDAIGGVVNIITTRDNPGTEL
TAGWGSNSYQNYDLSTQQQLGENTRATLIGDYEYTKGFDVVAKGGTGMQAQPDRDGFLSKTLYGALEHTFSDRWSGFVRG
YGYDNRTDYDAYYSPGSPLIDTRKLYSQSWDAGLRFNGERIQSQLVSSYSHSKDYNYDPHYGRYDTSATLDEMKQYNVQW
TNSVVVGHGNVGAGVDWQKQTTTPGTGYVPEGYDQRNTGVYLTGLQQLGDFTLEAAARSDDNSQFGRHGTWQTSAGWEFI
EGYRFIASYGTSYKAPNLGQLYGYYGNPNLNPEKSKQWEGAFEGLTAGVSWRISGYRNDINDMIDYDDHLQKYYNEGKAR
IKGIEATANFDTGPLTHTVSYDYVDARNAITDTPLPRRSKQMAKYQLDWDVYDFDWGVTYQYLGSRYDSDYSAYPYRTVK
MGGVSLWDLTVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=614, Percent_Identity=87.4592833876222, Blast_Score=1127, Evalue=0.0,
Organism=Escherichia coli, GI1788478, Length=701, Percent_Identity=25.962910128388, Blast_Score=164, Evalue=2e-41,
Organism=Escherichia coli, GI1786798, Length=715, Percent_Identity=24.3356643356643, Blast_Score=86, Evalue=8e-18,
Organism=Escherichia coli, GI1787024, Length=207, Percent_Identity=27.536231884058, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1790743, Length=112, Percent_Identity=35.7142857142857, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010101
- InterPro:   IPR010916
- InterPro:   IPR010917 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 68543; Mature: 68543

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00430 TONB_DEPENDENT_REC_1 ; PS01156 TONB_DEPENDENT_REC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKATLLTAFSVTAFSAWAQDTSPDTLVVTANRFQQPRSAVLAPVTIVTRQDIERWQST
CCCCEEEEEHHHHHHHHHHCCCCCCCEEEEECHHCCCCCHHHCCEEEEEEHHHHHHHHCC
SVNDVLRRLPGVDIAQSGGTGQNSSIFIRGTNSSHVLVLIDGVRLNLAGVSGSADLSQFP
CHHHHHHHCCCCCEECCCCCCCCCEEEEEECCCCEEEEEEECEEEEEEECCCCCCHHHHH
VSLVQRIEYIRGPRSAIYGSDAIGGVVNIITTRDNPGTELTAGWGSNSYQNYDLSTQQQL
HHHHHHHHHHCCCCHHCCCCCCCCCEEEEEEECCCCCCEEEECCCCCCCCCCCCCHHHHH
GENTRATLIGDYEYTKGFDVVAKGGTGMQAQPDRDGFLSKTLYGALEHTFSDRWSGFVRG
CCCCCEEEEECCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEE
YGYDNRTDYDAYYSPGSPLIDTRKLYSQSWDAGLRFNGERIQSQLVSSYSHSKDYNYDPH
CCCCCCCCCCCEECCCCCCCHHHHHHHCCCCCCCEECHHHHHHHHHHHHCCCCCCCCCCC
YGRYDTSATLDEMKQYNVQWTNSVVVGHGNVGAGVDWQKQTTTPGTGYVPEGYDQRNTGV
CCCCCCCHHHHHHHHCCCEEECEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCE
YLTGLQQLGDFTLEAAARSDDNSQFGRHGTWQTSAGWEFIEGYRFIASYGTSYKAPNLGQ
EEEEHHHHCCEEEEHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCC
LYGYYGNPNLNPEKSKQWEGAFEGLTAGVSWRISGYRNDINDMIDYDDHLQKYYNEGKAR
EEEEECCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHCCHHHHHHHHHHCCCEE
IKGIEATANFDTGPLTHTVSYDYVDARNAITDTPLPRRSKQMAKYQLDWDVYDFDWGVTY
EEEEEEECCCCCCCCEEEEEECEECCCCCCCCCCCCCHHHHHHEEEECCEEEECCCCCCH
QYLGSRYDSDYSAYPYRTVKMGGVSLWDLTVAYPVTSHLTVRGKIANLFDKDYETVYGYQ
HHHCCCCCCCCCCCCEEEEEECCEEEEEEEEEEECCCEEEEEEHHHHHHCCCCHHEEEEE
TAGREYTLSGSYTF
CCCCEEEECCCCCC
>Mature Secondary Structure
MIKKATLLTAFSVTAFSAWAQDTSPDTLVVTANRFQQPRSAVLAPVTIVTRQDIERWQST
CCCCEEEEEHHHHHHHHHHCCCCCCCEEEEECHHCCCCCHHHCCEEEEEEHHHHHHHHCC
SVNDVLRRLPGVDIAQSGGTGQNSSIFIRGTNSSHVLVLIDGVRLNLAGVSGSADLSQFP
CHHHHHHHCCCCCEECCCCCCCCCEEEEEECCCCEEEEEEECEEEEEEECCCCCCHHHHH
VSLVQRIEYIRGPRSAIYGSDAIGGVVNIITTRDNPGTELTAGWGSNSYQNYDLSTQQQL
HHHHHHHHHHCCCCHHCCCCCCCCCEEEEEEECCCCCCEEEECCCCCCCCCCCCCHHHHH
GENTRATLIGDYEYTKGFDVVAKGGTGMQAQPDRDGFLSKTLYGALEHTFSDRWSGFVRG
CCCCCEEEEECCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEE
YGYDNRTDYDAYYSPGSPLIDTRKLYSQSWDAGLRFNGERIQSQLVSSYSHSKDYNYDPH
CCCCCCCCCCCEECCCCCCCHHHHHHHCCCCCCCEECHHHHHHHHHHHHCCCCCCCCCCC
YGRYDTSATLDEMKQYNVQWTNSVVVGHGNVGAGVDWQKQTTTPGTGYVPEGYDQRNTGV
CCCCCCCHHHHHHHHCCCEEECEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCE
YLTGLQQLGDFTLEAAARSDDNSQFGRHGTWQTSAGWEFIEGYRFIASYGTSYKAPNLGQ
EEEEHHHHCCEEEEHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCC
LYGYYGNPNLNPEKSKQWEGAFEGLTAGVSWRISGYRNDINDMIDYDDHLQKYYNEGKAR
EEEEECCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHCCHHHHHHHHHHCCCEE
IKGIEATANFDTGPLTHTVSYDYVDARNAITDTPLPRRSKQMAKYQLDWDVYDFDWGVTY
EEEEEEECCCCCCCCEEEEEECEECCCCCCCCCCCCCHHHHHHEEEECCEEEECCCCCCH
QYLGSRYDSDYSAYPYRTVKMGGVSLWDLTVAYPVTSHLTVRGKIANLFDKDYETVYGYQ
HHHCCCCCCCCCCCCEEEEEECCEEEEEEEEEEECCCEEEEEEHHHHHHCCCCHHEEEEE
TAGREYTLSGSYTF
CCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA