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The map label for this gene is yfiN [H]

Identifier: 29142996

GI number: 29142996

Start: 2704600

End: 2705820

Strand: Direct

Name: yfiN [H]

Synonym: t2627

Alternate gene names: 29142996

Gene position: 2704600-2705820 (Clockwise)

Preceding gene: 29142995

Following gene: 29143002

Centisome position: 56.44

GC content: 49.88

Gene sequence:

>1221_bases
ATGAATAAGGAATTTTCTCTGTCCAGACCAACATTTAAACGCACACTACGGCGGATTAGTATAATCAGCGTGCTGCTTAC
AATGACATTGATCTGGCTATTAATTTGCGTTGCGTCTGTCCTTACGCTCAAACAGTATGCGCAAAAGAATCTCGATTTGA
CCGCCGCCACAATGGCCCATAGCCTTGAAGCGGCACTGGTATTTTCCGATAGCGCGGCCGCAGCGGAAACGCTCGCCACA
CTGGGACGCCAGGGACAATTTTCAGCGGCGGAGGTCCGCGATAAAAATGGCCGTACTATCGCCTCATGGCGATATGATGC
GCGAGCCGCAGACGATAAGCTCATCGGCTTAATTAGCCACTGGCTTTTTCCATTGCCGGTATCGCAACCCGTCTGGCACA
ACGGCAGGGCTATCGGCGAAGTACGGCTTGTCGCCCGCGACAGCCTTATTGGTCATTTTATCTGGCTATCGCTGGCAGTG
CTGACAGGATGTATTCTGCTAGCATCCGGCATTGCCCTGCTACTCACGCGTTATTTACACAATGGCGTTGTGGATGCGCT
ACAAAATATTACTGAAATTGTACACGACGTTCGCACTAACCGAAATTTTTCACGCCGGGTACCTGATGAGCGTATTGCGG
AATTTCACCTGTTTGCGCAGGATTTCAATAGCCTTCTGGATGAGATGGAAGAATGGCAGCTACGGCTTCAGGCTAAAAAT
GCACAATTACTACGTACCGCGTTGCACGATCCGCTGACGGGGCTTGCCAATCGCGCGGCATTTCGCAGCTGTATTAACGC
GCTGATGAAGGACAATTCCGCTCGTAGCAGTTCGGCATTGTTATTTCTGGATGGCGATAACTTTAAATATATTAATGATA
CCTGGGGACATGCGGCAGGCGACCGCGTACTTATAGAGGTTGCCAAAAGATTAGCGGAATTCGGTGGTAGCCGTTATCAG
ACTTACCGACTCGGCGGCGATGAATTTGCGATGGTGCTTTACGGTGTACATTCGGAGTATGAAGTACAACGTATTTGCGC
AGCACTATCCCAGGCGTTTAATCGACCTTTTGAACTGCATAACGGCCAGCGAATAACGATGACCCTGAGTATTGGCTTTG
CGCTGACATGGGAACATGCCACTGCCGAAAAACTACAAGAACTGGCCGATCGAAATATGTATCAGGCTAAACACCGGCGT
GCAGAACGCTCGCTAAACTAA

Upstream 100 bases:

>100_bases
AACAATGATGAAGTCAAATTTTCCGTCAACCTGGACTCCCTCTCGCATAGCGGCGTGAGAGTGAATCCAGAAGTATTAAT
GCTTGCACGGAATCAAAAGC

Downstream 100 bases:

>100_bases
GGAACGGGCCAGGCCGTTTCTGACTCAGCGTTGATACCACAGACCCAGCTTAATCATGCAACCGTCTATGGCGTGGAGCA
ACCCATAAAAAAAGGGGCCA

Product: hypothetical protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MNKEFSLSRPTFKRTLRRISIISVLLTMTLIWLLICVASVLTLKQYAQKNLDLTAATMAHSLEAALVFSDSAAAAETLAT
LGRQGQFSAAEVRDKNGRTIASWRYDARAADDKLIGLISHWLFPLPVSQPVWHNGRAIGEVRLVARDSLIGHFIWLSLAV
LTGCILLASGIALLLTRYLHNGVVDALQNITEIVHDVRTNRNFSRRVPDERIAEFHLFAQDFNSLLDEMEEWQLRLQAKN
AQLLRTALHDPLTGLANRAAFRSCINALMKDNSARSSSALLFLDGDNFKYINDTWGHAAGDRVLIEVAKRLAEFGGSRYQ
TYRLGGDEFAMVLYGVHSEYEVQRICAALSQAFNRPFELHNGQRITMTLSIGFALTWEHATAEKLQELADRNMYQAKHRR
AERSLN

Sequences:

>Translated_406_residues
MNKEFSLSRPTFKRTLRRISIISVLLTMTLIWLLICVASVLTLKQYAQKNLDLTAATMAHSLEAALVFSDSAAAAETLAT
LGRQGQFSAAEVRDKNGRTIASWRYDARAADDKLIGLISHWLFPLPVSQPVWHNGRAIGEVRLVARDSLIGHFIWLSLAV
LTGCILLASGIALLLTRYLHNGVVDALQNITEIVHDVRTNRNFSRRVPDERIAEFHLFAQDFNSLLDEMEEWQLRLQAKN
AQLLRTALHDPLTGLANRAAFRSCINALMKDNSARSSSALLFLDGDNFKYINDTWGHAAGDRVLIEVAKRLAEFGGSRYQ
TYRLGGDEFAMVLYGVHSEYEVQRICAALSQAFNRPFELHNGQRITMTLSIGFALTWEHATAEKLQELADRNMYQAKHRR
AERSLN
>Mature_406_residues
MNKEFSLSRPTFKRTLRRISIISVLLTMTLIWLLICVASVLTLKQYAQKNLDLTAATMAHSLEAALVFSDSAAAAETLAT
LGRQGQFSAAEVRDKNGRTIASWRYDARAADDKLIGLISHWLFPLPVSQPVWHNGRAIGEVRLVARDSLIGHFIWLSLAV
LTGCILLASGIALLLTRYLHNGVVDALQNITEIVHDVRTNRNFSRRVPDERIAEFHLFAQDFNSLLDEMEEWQLRLQAKN
AQLLRTALHDPLTGLANRAAFRSCINALMKDNSARSSSALLFLDGDNFKYINDTWGHAAGDRVLIEVAKRLAEFGGSRYQ
TYRLGGDEFAMVLYGVHSEYEVQRICAALSQAFNRPFELHNGQRITMTLSIGFALTWEHATAEKLQELADRNMYQAKHRR
AERSLN

Specific function: Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HAMP domain [H]

Homologues:

Organism=Escherichia coli, GI1788956, Length=404, Percent_Identity=74.7524752475248, Blast_Score=628, Evalue=0.0,
Organism=Escherichia coli, GI145693134, Length=152, Percent_Identity=35.5263157894737, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1788381, Length=113, Percent_Identity=41.5929203539823, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1787056, Length=314, Percent_Identity=28.0254777070064, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1787816, Length=156, Percent_Identity=29.4871794871795, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1786584, Length=158, Percent_Identity=29.746835443038, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787262, Length=153, Percent_Identity=29.4117647058824, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87082007, Length=175, Percent_Identity=30.8571428571429, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI87081977, Length=99, Percent_Identity=39.3939393939394, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87081881, Length=170, Percent_Identity=30.5882352941176, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787541, Length=150, Percent_Identity=30, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR003660 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 45696; Mature: 45696

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: PS50885 HAMP ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKEFSLSRPTFKRTLRRISIISVLLTMTLIWLLICVASVLTLKQYAQKNLDLTAATMAH
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
SLEAALVFSDSAAAAETLATLGRQGQFSAAEVRDKNGRTIASWRYDARAADDKLIGLISH
HHHHHEEECCCHHHHHHHHHHCCCCCCCHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHH
WLFPLPVSQPVWHNGRAIGEVRLVARDSLIGHFIWLSLAVLTGCILLASGIALLLTRYLH
HHCCCCCCCCCCCCCCCHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NGVVDALQNITEIVHDVRTNRNFSRRVPDERIAEFHLFAQDFNSLLDEMEEWQLRLQAKN
HHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQLLRTALHDPLTGLANRAAFRSCINALMKDNSARSSSALLFLDGDNFKYINDTWGHAAG
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCEEEECCCCCCCCC
DRVLIEVAKRLAEFGGSRYQTYRLGGDEFAMVLYGVHSEYEVQRICAALSQAFNRPFELH
CHHHHHHHHHHHHCCCCCEEEEEECCCCEEEHEEECCCHHHHHHHHHHHHHHHCCCEECC
NGQRITMTLSIGFALTWEHATAEKLQELADRNMYQAKHRRAERSLN
CCCEEEEEEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNKEFSLSRPTFKRTLRRISIISVLLTMTLIWLLICVASVLTLKQYAQKNLDLTAATMAH
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
SLEAALVFSDSAAAAETLATLGRQGQFSAAEVRDKNGRTIASWRYDARAADDKLIGLISH
HHHHHEEECCCHHHHHHHHHHCCCCCCCHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHH
WLFPLPVSQPVWHNGRAIGEVRLVARDSLIGHFIWLSLAVLTGCILLASGIALLLTRYLH
HHCCCCCCCCCCCCCCCHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NGVVDALQNITEIVHDVRTNRNFSRRVPDERIAEFHLFAQDFNSLLDEMEEWQLRLQAKN
HHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQLLRTALHDPLTGLANRAAFRSCINALMKDNSARSSSALLFLDGDNFKYINDTWGHAAG
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCEEEECCCCCCCCC
DRVLIEVAKRLAEFGGSRYQTYRLGGDEFAMVLYGVHSEYEVQRICAALSQAFNRPFELH
CHHHHHHHHHHHHCCCCCEEEEEECCCCEEEHEEECCCHHHHHHHHHHHHHHHCCCEECC
NGQRITMTLSIGFALTWEHATAEKLQELADRNMYQAKHRRAERSLN
CCCEEEEEEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 6357787; 7567469 [H]