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The map label for this gene is pheA [H]

Identifier: 29142991

GI number: 29142991

Start: 2699361

End: 2700521

Strand: Direct

Name: pheA [H]

Synonym: t2622

Alternate gene names: 29142991

Gene position: 2699361-2700521 (Clockwise)

Preceding gene: 29142990

Following gene: 29142995

Centisome position: 56.33

GC content: 53.92

Gene sequence:

>1161_bases
ATGACATCGGAAAACCCATTACTGGCGCTGCGAGATAAAATCAGCGCTTTAGACGAAGAGTTACTGGCCTTACTGGCAAA
ACGACGCGCGCTGGCGATTGAAGTGGGACAAGCAAAACTACTGTCGCATCGTCCGGTTCGGGATATCGATCGTGAACGCG
CGCTGCTGGACAGACTCATCCATCTCGGTAAAGCCCACCATCTCGACGCACACTACATTACCCGTCTGTTCCAGCTTATC
ATTGAAGACTCCGTGCTTACTCAGCAGGCGCTGCTGCAACAACATCTGAATAATACTCACCCTCATTCGGCACGTATTGC
GTTTCTTGGGCCGAAAGGCTCCTATTCTCATCTCGCGGCGCGCCAGTATGCTGCACGCCATTTTGAGCAATTTATTGAGA
GCGGTTGCGCAAAATTCACCGATATTTTTCATCAGGTCGAAACCGGCCAGGCCGATTACGCCGTGGTTCCGATAGAGAAC
ACCAACTCCGGCGCTATCAACGATGTGTACGACTTATTGCAACACACCAGTCTGTCGATTGTCGGTGAGATGACCGTCAC
TATCGATCACTGCGTGCTGGTTTCCGGCGCTACGGATCTGAATACCATCGAAACGGTGTACAGCCATCCGCAGCCGTTTC
AGCAGTGCAGTAAATTTTTGAGCCGCTATCCGCACTGGAAAATCGACTATACCGAGAGTACGTCGGCAGCGATGGAAAAA
GTCGCGCAGGCAAACTCTCCGCGCGTCGCGGCGCTCGGCAGCGAGGCAGGCGGCATGTTGCACGGTTTACAGGTGCTGGA
ACGCATCGCCGCAAACCAGACACAGAATATCACCCGCTTTCTGGTACTGGCGCGCAAAGCCATCAACGTTTCCGATCAGG
TTCCGGCAAAAACCACTCTGTTAATCGCCACCGGGCAGCAAGCTGGCGCGCTGGTCGAAGCGCTGCTGGTGCTGCGTAAC
CACAATCTCATCATGACGAAACTGGAGTCGCGCCCCATTCACGGCAATCCGTGGGAAGAGATGTTTTATCTCGATATTCA
GGCGAACCTGGAGTCGCAGGTAATGCAAAGCGCGCTAAAAGAGCTGGGCGAGATCACGCGCTCAATGAAAGTGCTTGGCT
GCTATCCCAGCGAAAACGTCGTGCCGGTAGAACCTGCCTGA

Upstream 100 bases:

>100_bases
ACCGGGAGGCGTTTCGTCATGTGATAAAGAATGCGAAGACGAACAAGAAGGCCTCCCCCACCGGGAGGCCTTTTTTATTG
ATAACAAAAAAGGCAACACT

Downstream 100 bases:

>100_bases
CGTTCAATAAAACGGCTTTTCTTCATCCCTGCGACGGCTACCTGCAGGGTGAAGATGGCGCCGGAGAGCGGATAATCCGC
CACTTCCTGAGCAGACATAT

Product: bifunctional chorismate mutase/prephenate dehydratase

Products: NA

Alternate protein names: Chorismate mutase; CM; Prephenate dehydratase; PDT [H]

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MTSENPLLALRDKISALDEELLALLAKRRALAIEVGQAKLLSHRPVRDIDRERALLDRLIHLGKAHHLDAHYITRLFQLI
IEDSVLTQQALLQQHLNNTHPHSARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFTDIFHQVETGQADYAVVPIEN
TNSGAINDVYDLLQHTSLSIVGEMTVTIDHCVLVSGATDLNTIETVYSHPQPFQQCSKFLSRYPHWKIDYTESTSAAMEK
VAQANSPRVAALGSEAGGMLHGLQVLERIAANQTQNITRFLVLARKAINVSDQVPAKTTLLIATGQQAGALVEALLVLRN
HNLIMTKLESRPIHGNPWEEMFYLDIQANLESQVMQSALKELGEITRSMKVLGCYPSENVVPVEPA

Sequences:

>Translated_386_residues
MTSENPLLALRDKISALDEELLALLAKRRALAIEVGQAKLLSHRPVRDIDRERALLDRLIHLGKAHHLDAHYITRLFQLI
IEDSVLTQQALLQQHLNNTHPHSARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFTDIFHQVETGQADYAVVPIEN
TNSGAINDVYDLLQHTSLSIVGEMTVTIDHCVLVSGATDLNTIETVYSHPQPFQQCSKFLSRYPHWKIDYTESTSAAMEK
VAQANSPRVAALGSEAGGMLHGLQVLERIAANQTQNITRFLVLARKAINVSDQVPAKTTLLIATGQQAGALVEALLVLRN
HNLIMTKLESRPIHGNPWEEMFYLDIQANLESQVMQSALKELGEITRSMKVLGCYPSENVVPVEPA
>Mature_385_residues
TSENPLLALRDKISALDEELLALLAKRRALAIEVGQAKLLSHRPVRDIDRERALLDRLIHLGKAHHLDAHYITRLFQLII
EDSVLTQQALLQQHLNNTHPHSARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFTDIFHQVETGQADYAVVPIENT
NSGAINDVYDLLQHTSLSIVGEMTVTIDHCVLVSGATDLNTIETVYSHPQPFQQCSKFLSRYPHWKIDYTESTSAAMEKV
AQANSPRVAALGSEAGGMLHGLQVLERIAANQTQNITRFLVLARKAINVSDQVPAKTTLLIATGQQAGALVEALLVLRNH
NLIMTKLESRPIHGNPWEEMFYLDIQANLESQVMQSALKELGEITRSMKVLGCYPSENVVPVEPA

Specific function: L-phenylalanine biosynthesis. [C]

COG id: COG0077

COG function: function code E; Prephenate dehydratase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 prephenate dehydratase domain [H]

Homologues:

Organism=Escherichia coli, GI1788951, Length=385, Percent_Identity=90.6493506493507, Blast_Score=727, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6324013, Length=323, Percent_Identity=26.9349845201238, Blast_Score=102, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008242
- InterPro:   IPR002701
- InterPro:   IPR020822
- InterPro:   IPR010952
- InterPro:   IPR001086
- InterPro:   IPR018528 [H]

Pfam domain/function: PF01817 CM_2; PF00800 PDT [H]

EC number: =5.4.99.5; =4.2.1.51 [H]

Molecular weight: Translated: 42852; Mature: 42720

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS00858 PREPHENATE_DEHYDR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSENPLLALRDKISALDEELLALLAKRRALAIEVGQAKLLSHRPVRDIDRERALLDRLI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHCCCCHHHHHHHHHHHHHHH
HLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNNTHPHSARIAFLGPKGSYSHLAA
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHH
RQYAARHFEQFIESGCAKFTDIFHQVETGQADYAVVPIENTNSGAINDVYDLLQHTSLSI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHCCHHH
VGEMTVTIDHCVLVSGATDLNTIETVYSHPQPFQQCSKFLSRYPHWKIDYTESTSAAMEK
HEEEEEEEEEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCEEECCCCHHHHHHH
VAQANSPRVAALGSEAGGMLHGLQVLERIAANQTQNITRFLVLARKAINVSDQVPAKTTL
HHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCEEE
LIATGQQAGALVEALLVLRNHNLIMTKLESRPIHGNPWEEMFYLDIQANLESQVMQSALK
EEECCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHEEEEEEECCHHHHHHHHHHH
ELGEITRSMKVLGCYPSENVVPVEPA
HHHHHHHHHEEEEECCCCCEEECCCC
>Mature Secondary Structure 
TSENPLLALRDKISALDEELLALLAKRRALAIEVGQAKLLSHRPVRDIDRERALLDRLI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHCCCCHHHHHHHHHHHHHHH
HLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNNTHPHSARIAFLGPKGSYSHLAA
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHH
RQYAARHFEQFIESGCAKFTDIFHQVETGQADYAVVPIENTNSGAINDVYDLLQHTSLSI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHCCHHH
VGEMTVTIDHCVLVSGATDLNTIETVYSHPQPFQQCSKFLSRYPHWKIDYTESTSAAMEK
HEEEEEEEEEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCEEECCCCHHHHHHH
VAQANSPRVAALGSEAGGMLHGLQVLERIAANQTQNITRFLVLARKAINVSDQVPAKTTL
HHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCEEE
LIATGQQAGALVEALLVLRNHNLIMTKLESRPIHGNPWEEMFYLDIQANLESQVMQSALK
EEECCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHEEEEEEECCHHHHHHHHHHH
ELGEITRSMKVLGCYPSENVVPVEPA
HHHHHHHHHEEEEECCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]