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The map label for this gene is hpcH [H]

Identifier: 29142244

GI number: 29142244

Start: 1876760

End: 1877551

Strand: Reverse

Name: hpcH [H]

Synonym: t1813

Alternate gene names: 29142244

Gene position: 1877551-1876760 (Counterclockwise)

Preceding gene: 29142245

Following gene: 29142243

Centisome position: 39.18

GC content: 60.86

Gene sequence:

>792_bases
ATGAAAAATGCTTTCAAAGACGCGTTAAAAGCGGGGCGCCCGCAAATCGGTTTGTGGCTGGGGCTTGCCAACAGTTACAG
CGCTGAACTGTTAGCGGGCGCCGGCTTCGACTGGCTACTGATTGACGGTGAACACGCGCCAAACAACGTGCAGACGGTGT
TGACCCAGTTGCAGGCGATTGCGCCTTATCCCAGCCAGCCGGTGGTGCGTCCGTCATGGAACGATCCGGTACAGATTAAG
CAACTGCTCGACGTCGGCGCGCAAACGCTGCTGATACCGATGGTGCAGAATGCCGATGAAGCGCGAAACGCCGTGGCGGC
TACGCGTTATCCGCCTGCCGGTATTCGCGGCGTGGGCAGCGCGCTGGCGCGGGCATCGCGCTGGAATCGCATTCCGGACT
ATCTCCACCAGGCCAACGACGCCATGTGCGTACTGGTGCAGATTGAAACGCGTGAGGCGATGAGCAATCTGGCGTCAATT
CTCGACGTGGATGGCATTGACGGCGTGTTTATTGGCCCGGCGGATCTCAGCGCCGATATGGGCTTTGCCGGCAATCCGCA
GCACCCGGAAGTGCAGGCGGCGATTGAGAACGCCATCGTGCAGATACGCGCGGCGGGGAAAGCGCCGGGGATTCTGATGG
CCAATGAAGCACTGGCGAAACGTTATCTGGAACTGGGGGCGCTATTTGTCGCCGTCGGCGTTGACACCACGCTGCTGGCG
CGCGGAGCGGAGGCGCTGGCGGCGCGCTTTGGCGCAGAAAAAAAACTGTCCGGTGCGTCCGGCGTCTATTAA

Upstream 100 bases:

>100_bases
TCGTTCACCCGCCCGGTGCCGGCGCGCAAGGGCGACACCTTCCATGTCGATTACGGCAACATGGGCGCGATCAGTTGCCG
GTTTGTGTAAGGAAAAAACG

Downstream 100 bases:

>100_bases
GCCTGGGCCGGTAAGCGCAGCGCTACCGGGCAACCGTAGTACCCTACAAAATTCCCATCAGAGGAAAAAAAATGAGCGAC
ACATCATCTGCACTTCCGGA

Product: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Products: NA

Alternate protein names: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase; HHED aldolase; 4-hydroxy-2-ketoheptane-1,7-dioate aldolase; HKHD aldolase [H]

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MKNAFKDALKAGRPQIGLWLGLANSYSAELLAGAGFDWLLIDGEHAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIK
QLLDVGAQTLLIPMVQNADEARNAVAATRYPPAGIRGVGSALARASRWNRIPDYLHQANDAMCVLVQIETREAMSNLASI
LDVDGIDGVFIGPADLSADMGFAGNPQHPEVQAAIENAIVQIRAAGKAPGILMANEALAKRYLELGALFVAVGVDTTLLA
RGAEALAARFGAEKKLSGASGVY

Sequences:

>Translated_263_residues
MKNAFKDALKAGRPQIGLWLGLANSYSAELLAGAGFDWLLIDGEHAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIK
QLLDVGAQTLLIPMVQNADEARNAVAATRYPPAGIRGVGSALARASRWNRIPDYLHQANDAMCVLVQIETREAMSNLASI
LDVDGIDGVFIGPADLSADMGFAGNPQHPEVQAAIENAIVQIRAAGKAPGILMANEALAKRYLELGALFVAVGVDTTLLA
RGAEALAARFGAEKKLSGASGVY
>Mature_263_residues
MKNAFKDALKAGRPQIGLWLGLANSYSAELLAGAGFDWLLIDGEHAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIK
QLLDVGAQTLLIPMVQNADEARNAVAATRYPPAGIRGVGSALARASRWNRIPDYLHQANDAMCVLVQIETREAMSNLASI
LDVDGIDGVFIGPADLSADMGFAGNPQHPEVQAAIENAIVQIRAAGKAPGILMANEALAKRYLELGALFVAVGVDTTLLA
RGAEALAARFGAEKKLSGASGVY

Specific function: Catalyzes the reversible retro-aldol cleavage of 4- hydroxy-2-ketoheptane-1,7-dioate (HKHD) to pyruvate and succinic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788578, Length=263, Percent_Identity=55.5133079847909, Blast_Score=294, Evalue=3e-81,
Organism=Escherichia coli, GI1789514, Length=248, Percent_Identity=48.7903225806452, Blast_Score=234, Evalue=5e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR012689
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.2.-

Molecular weight: Translated: 27787; Mature: 27787

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNAFKDALKAGRPQIGLWLGLANSYSAELLAGAGFDWLLIDGEHAPNNVQTVLTQLQAI
CCHHHHHHHHCCCCCEEEEEEECCCCCHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHH
APYPSQPVVRPSWNDPVQIKQLLDVGAQTLLIPMVQNADEARNAVAATRYPPAGIRGVGS
CCCCCCCCCCCCCCCHHHHHHHHHCCHHHEEHHHHCCHHHHHHHHHHCCCCCCCHHHHHH
ALARASRWNRIPDYLHQANDAMCVLVQIETREAMSNLASILDVDGIDGVFIGPADLSADM
HHHHHHHCCCCHHHHHCCCCEEEEEEEECHHHHHHHHHHHHCCCCCCEEEECCCCCCCCC
GFAGNPQHPEVQAAIENAIVQIRAAGKAPGILMANEALAKRYLELGALFVAVGVDTTLLA
CCCCCCCCCHHHHHHHHHHEEEEECCCCCCEEEECHHHHHHHHHHHHHHHHHCCCHHHHH
RGAEALAARFGAEKKLSGASGVY
HHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKNAFKDALKAGRPQIGLWLGLANSYSAELLAGAGFDWLLIDGEHAPNNVQTVLTQLQAI
CCHHHHHHHHCCCCCEEEEEEECCCCCHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHH
APYPSQPVVRPSWNDPVQIKQLLDVGAQTLLIPMVQNADEARNAVAATRYPPAGIRGVGS
CCCCCCCCCCCCCCCHHHHHHHHHCCHHHEEHHHHCCHHHHHHHHHHCCCCCCCHHHHHH
ALARASRWNRIPDYLHQANDAMCVLVQIETREAMSNLASILDVDGIDGVFIGPADLSADM
HHHHHHHCCCCHHHHHCCCCEEEEEEEECHHHHHHHHHHHHCCCCCCEEEECCCCCCCCC
GFAGNPQHPEVQAAIENAIVQIRAAGKAPGILMANEALAKRYLELGALFVAVGVDTTLLA
CCCCCCCCCHHHHHHHHHHEEEEECCCCCCEEEECHHHHHHHHHHHHHHHHHCCCHHHHH
RGAEALAARFGAEKKLSGASGVY
HHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA