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The map label for this gene is thiK

Identifier: 29142146

GI number: 29142146

Start: 1778922

End: 1779746

Strand: Reverse

Name: thiK

Synonym: t1712

Alternate gene names: 29142146

Gene position: 1779746-1778922 (Counterclockwise)

Preceding gene: 29142147

Following gene: 29142145

Centisome position: 37.14

GC content: 57.09

Gene sequence:

>825_bases
GTGCGGTCCAACAACAATAATCCCTTAACGCGCGACGAGATCCTGTCGCGCTATTTTCCCCAGTATCGTCCCGCCGTCGC
CGCATCGCAGGGGCTAAGCGGCGGGAGCTGTATTATTGCTCACGATACTCACCGTATCGTGCTGCGCCGTCATCACGACC
CCGACGCTCCACCAGCCCATTTTTTACGTCATCACCGCGCCTTATCCCAATTGCCGGCCAGTCTGGCGCCGCGAGCGCTT
TTTTATACGCCAGGCTGGATGGCGGTAGAATATCTGCATGGTGTGGTAAATTCCGCTCTGCCAGATGCCGACGAACTGGC
GGCCTTACTGTATCATTTGCATCAACAGCCGCGTTTTGGCTGGCGTATCGCGCTATCGCCACTATTGGCGCAGTACTGGT
CGTGTTGCGATCCGGCAAGGCGTACGCCGTTTTGGTTGCGGCGGCTCAAACAGTTGCAAAAAAACGGTGAACCTCGCCCG
CTGCGGCTCGCGCCTTTGCATATGGATGTCCATGGCGACAATATAGTATTAACGTCCGCCGGGTTGAGACTGATTGACTG
GGAGTATGCCGGCGACGGCGATATTGCGTTGGAGCTGGCGGCAGTATGGGTTGAGGATGAACGCCAGCACCGACAACTGG
CAGACGCTTATGCCGCGCGCGCGCGAATCGACGCCCGGCAGCTATGGCGACAGATACGATTATGGCACCCCTGGGTCATT
ATGCTAAAAGCAGGGTGGTTCGAATACCGCTGGCGACAAACCGGCGAGCAACAATTTATCAGGCTGGCCGATGAAACCTG
GCGCCAGCTACGTATGAAAGGATAA

Upstream 100 bases:

>100_bases
TATGTACTTTACTCCAGCGCATCCGGTAACGTTAACGCGCCTGCATTACAGATGCAGTTAATGCTGGTGCAAACTGGCGA
GATAATCTGGTCAGGTAAAG

Downstream 100 bases:

>100_bases
GGAGAGCAGTGTGGGCCCAGTAATGTTGAATGTCGAAGGATGTGAGCTGGATGCGGAGGAGCGCGAGATACTGGCGCATC
CGCTGGTTGGCGGGCTTATT

Product: thiamine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MRSNNNNPLTRDEILSRYFPQYRPAVAASQGLSGGSCIIAHDTHRIVLRRHHDPDAPPAHFLRHHRALSQLPASLAPRAL
FYTPGWMAVEYLHGVVNSALPDADELAALLYHLHQQPRFGWRIALSPLLAQYWSCCDPARRTPFWLRRLKQLQKNGEPRP
LRLAPLHMDVHGDNIVLTSAGLRLIDWEYAGDGDIALELAAVWVEDERQHRQLADAYAARARIDARQLWRQIRLWHPWVI
MLKAGWFEYRWRQTGEQQFIRLADETWRQLRMKG

Sequences:

>Translated_274_residues
MRSNNNNPLTRDEILSRYFPQYRPAVAASQGLSGGSCIIAHDTHRIVLRRHHDPDAPPAHFLRHHRALSQLPASLAPRAL
FYTPGWMAVEYLHGVVNSALPDADELAALLYHLHQQPRFGWRIALSPLLAQYWSCCDPARRTPFWLRRLKQLQKNGEPRP
LRLAPLHMDVHGDNIVLTSAGLRLIDWEYAGDGDIALELAAVWVEDERQHRQLADAYAARARIDARQLWRQIRLWHPWVI
MLKAGWFEYRWRQTGEQQFIRLADETWRQLRMKG
>Mature_274_residues
MRSNNNNPLTRDEILSRYFPQYRPAVAASQGLSGGSCIIAHDTHRIVLRRHHDPDAPPAHFLRHHRALSQLPASLAPRAL
FYTPGWMAVEYLHGVVNSALPDADELAALLYHLHQQPRFGWRIALSPLLAQYWSCCDPARRTPFWLRRLKQLQKNGEPRP
LRLAPLHMDVHGDNIVLTSAGLRLIDWEYAGDGDIALELAAVWVEDERQHRQLADAYAARARIDARQLWRQIRLWHPWVI
MLKAGWFEYRWRQTGEQQFIRLADETWRQLRMKG

Specific function: Catalyzes the phosphorylation of thiamine to thiamine phosphate

COG id: COG0510

COG function: function code M; Predicted choline kinase involved in LPS biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiamine kinase family

Homologues:

Organism=Escherichia coli, GI1787349, Length=273, Percent_Identity=66.3003663003663, Blast_Score=375, Evalue=1e-105,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIK_SALA4 (B5F8E6)

Other databases:

- EMBL:   CP001138
- RefSeq:   YP_002146834.1
- ProteinModelPortal:   B5F8E6
- SMR:   B5F8E6
- GeneID:   6796572
- GenomeReviews:   CP001138_GR
- KEGG:   sea:SeAg_B1977
- HOGENOM:   HBG614243
- OMA:   KAWQPRT
- ProtClustDB:   PRK10271
- HAMAP:   MF_01604
- InterPro:   IPR002575
- InterPro:   IPR011009
- InterPro:   IPR014093
- TIGRFAMs:   TIGR02721

Pfam domain/function: PF01636 APH; SSF56112 Kinase_like

EC number: =2.7.1.89

Molecular weight: Translated: 31899; Mature: 31899

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSNNNNPLTRDEILSRYFPQYRPAVAASQGLSGGSCIIAHDTHRIVLRRHHDPDAPPAH
CCCCCCCCCCHHHHHHHHCHHHCCHHHHHCCCCCCEEEEEECCCEEEEEECCCCCCCHHH
FLRHHRALSQLPASLAPRALFYTPGWMAVEYLHGVVNSALPDADELAALLYHLHQQPRFG
HHHHHHHHHHCCHHHCCCEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCC
WRIALSPLLAQYWSCCDPARRTPFWLRRLKQLQKNGEPRPLRLAPLHMDVHGDNIVLTSA
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCEEEEEEC
GLRLIDWEYAGDGDIALELAAVWVEDERQHRQLADAYAARARIDARQLWRQIRLWHPWVI
CCEEEEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
MLKAGWFEYRWRQTGEQQFIRLADETWRQLRMKG
EEECCHHHHHHHHCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRSNNNNPLTRDEILSRYFPQYRPAVAASQGLSGGSCIIAHDTHRIVLRRHHDPDAPPAH
CCCCCCCCCCHHHHHHHHCHHHCCHHHHHCCCCCCEEEEEECCCEEEEEECCCCCCCHHH
FLRHHRALSQLPASLAPRALFYTPGWMAVEYLHGVVNSALPDADELAALLYHLHQQPRFG
HHHHHHHHHHCCHHHCCCEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCC
WRIALSPLLAQYWSCCDPARRTPFWLRRLKQLQKNGEPRPLRLAPLHMDVHGDNIVLTSA
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCEEEEEEC
GLRLIDWEYAGDGDIALELAAVWVEDERQHRQLADAYAARARIDARQLWRQIRLWHPWVI
CCEEEEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
MLKAGWFEYRWRQTGEQQFIRLADETWRQLRMKG
EEECCHHHHHHHHCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA