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The map label for this gene is narK [H]

Identifier: 29142108

GI number: 29142108

Start: 1737487

End: 1738884

Strand: Reverse

Name: narK [H]

Synonym: t1674

Alternate gene names: 29142108

Gene position: 1738884-1737487 (Counterclockwise)

Preceding gene: 29142111

Following gene: 29142107

Centisome position: 36.29

GC content: 54.51

Gene sequence:

>1398_bases
ATGAGTCACTCATCCGCCCCGGAAAGGGCAACTGGCGCTGTTATCACGGAATGGCGTCCGGAAGATCCGGCGTTCTGGCA
GCAGCGCGGCCATCGTGTCGCTAGCCGTAATTTGTGGATTTCTGTCCCGTGTTTACTGCTGGCGTTTTGCGTCTGGATGC
TATTCAGCGCAGTGGCGGTGAACCTGCCGAAAGTGGGATTTAATTTTACGACCGATCAGCTCTTTATGTTGACTGCGCTG
CCATCTGTTTCCGGCGCGCTGCTACGGGTGCCTTACTCCTTTATGGTGCCGCTATTTGGCGGACGTCGCTGGACAGCGTT
CAGTACCGGTATCCTCATCGTGCCTTGCGTATGGTTAGGCTTTGCCGTTCAGGATACCAGTACGCCATTTAGCACTTTCA
TTATTATTTCCTTGCTGTGTGGTTTTGCCGGCGCGAACTTTGCCTCCAGTATGGCGAACATCAGCTTTTTCTTTCCTAAA
CAAAAGCAGGGCGGCGCGCTGGGTCTGAACGGCGGTCTGGGCAATATGGGCGTGAGCGTGATGCAGCTGGTTGCGCCGCT
GGTGGTTTCGCTCTCTATCTTTGCCGCCTTTGGCAGCCACGGTGTAGAGCAGCCGGACGGTTCTCAGCTCTATCTGGCGA
ACGCAGCCTGGATTTGGGTGCCTTTCCTGGCCATTTTTACGCTGGCAGCGTGGTTCGGCATGAACGAACTGGCGACGTCT
AAGGCCTCGCTAAAAGAGCAACTGCCGGTGCTCAAACGTGGTCATCTGTGGATCATGAGTCTGCTGTATCTGGCGACCTT
CGGATCGTTTATCGGTTTCTCCGCGGGGTTCGCTATGCTGTCTAAAACGCAATTCCCGGATGTGCAGATCCTGCATTATG
CGTTTTTCGGTCCGTTTATCGGCGCGCTGGCGCGTTCGGCAGGCGGCGCAATTTCTGACCGTCTGGGGGGGACTCGCGTC
ACGCTTATCAACTTTGTCCTGATGGCGATTTTCAGCGGTCTGCTGTTCCTGACATTACCTACTGGCGGCGTTGGCGGTAG
CTTTATCGCATTCTACGGGGTATTTCTGGCGCTATTCCTGACGGCGGGGCTGGGGAGTGGTTCTACCTTCCAGATGATCT
CCGTTATCTTCCGTAAACTGACGATGGACCGGGTAAAAGCGGAAGGCGGGTCGGACGAAAGAGCCATGCGTGAAGCGGCG
ACCGATACGGCGGCGGCGCTGGGCTTTATTTCCGCGATTGGCGCGATTGGCGGCTTTTTTATTCCGAAAGCGTTTGGTTC
ATCTTTAGCATTAACCGGTTCGCCAGTCGGCGCGATGAAAGTCTTTCTTATTTTCTATATCGCCTGCGTCGTTATTACCT
GGGCGGTATATGGACGTCATTCTAAAAATAAAAAGTAA

Upstream 100 bases:

>100_bases
TAGTCGCCGGTGATGACGACTTTTCCTTTGATTTATATCAACTTACCTTCGGCAGTAAACCCTAATGTGGCAGACATCAA
ATCAAGAATCAGAGGTGTCT

Downstream 100 bases:

>100_bases
CTTTTGATTATCCTTTGCGCGGCGTTAGACCGCGCTTTTTTTTACCCGCATTTAGTTACAACATACTTATAGTCAAGAAG
AGAGGCGCGACAGGGCTGAC

Product: nitrite extrusion protein

Products: NA

Alternate protein names: Nitrite facilitator 1 [H]

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MSHSSAPERATGAVITEWRPEDPAFWQQRGHRVASRNLWISVPCLLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTAL
PSVSGALLRVPYSFMVPLFGGRRWTAFSTGILIVPCVWLGFAVQDTSTPFSTFIIISLLCGFAGANFASSMANISFFFPK
QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAAFGSHGVEQPDGSQLYLANAAWIWVPFLAIFTLAAWFGMNELATS
KASLKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAGFAMLSKTQFPDVQILHYAFFGPFIGALARSAGGAISDRLGGTRV
TLINFVLMAIFSGLLFLTLPTGGVGGSFIAFYGVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSDERAMREAA
TDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACVVITWAVYGRHSKNKK

Sequences:

>Translated_465_residues
MSHSSAPERATGAVITEWRPEDPAFWQQRGHRVASRNLWISVPCLLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTAL
PSVSGALLRVPYSFMVPLFGGRRWTAFSTGILIVPCVWLGFAVQDTSTPFSTFIIISLLCGFAGANFASSMANISFFFPK
QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAAFGSHGVEQPDGSQLYLANAAWIWVPFLAIFTLAAWFGMNELATS
KASLKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAGFAMLSKTQFPDVQILHYAFFGPFIGALARSAGGAISDRLGGTRV
TLINFVLMAIFSGLLFLTLPTGGVGGSFIAFYGVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSDERAMREAA
TDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACVVITWAVYGRHSKNKK
>Mature_464_residues
SHSSAPERATGAVITEWRPEDPAFWQQRGHRVASRNLWISVPCLLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALP
SVSGALLRVPYSFMVPLFGGRRWTAFSTGILIVPCVWLGFAVQDTSTPFSTFIIISLLCGFAGANFASSMANISFFFPKQ
KQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAAFGSHGVEQPDGSQLYLANAAWIWVPFLAIFTLAAWFGMNELATSK
ASLKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAGFAMLSKTQFPDVQILHYAFFGPFIGALARSAGGAISDRLGGTRVT
LINFVLMAIFSGLLFLTLPTGGVGGSFIAFYGVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSDERAMREAAT
DTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACVVITWAVYGRHSKNKK

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=463, Percent_Identity=94.3844492440605, Blast_Score=850, Evalue=0.0,
Organism=Escherichia coli, GI1787743, Length=458, Percent_Identity=74.8908296943231, Blast_Score=655, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 49788; Mature: 49657

Theoretical pI: Translated: 10.23; Mature: 10.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHSSAPERATGAVITEWRPEDPAFWQQRGHRVASRNLWISVPCLLLAFCVWMLFSAVAV
CCCCCCCCCCCCCEEECCCCCCCHHHHHHCCCHHHCCCEEEHHHHHHHHHHHHHHHHHHH
NLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPLFGGRRWTAFSTGILIVPCVWLG
CCCCCCCCCCHHHHHHHHHCCCCCCHHHHCCHHHHHHHCCCCEEEECCCCHHHHHHHHHH
FAVQDTSTPFSTFIIISLLCGFAGANFASSMANISFFFPKQKQGGALGLNGGLGNMGVSV
HEECCCCCCHHHHHHHHHHHHHCCCHHHHHHCCEEEECCCCCCCCEEEECCCCCHHHHHH
MQLVAPLVVSLSIFAAFGSHGVEQPDGSQLYLANAAWIWVPFLAIFTLAAWFGMNELATS
HHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHCHHHHHHH
KASLKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAGFAMLSKTQFPDVQILHYAFFGPFI
HHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
GALARSAGGAISDRLGGTRVTLINFVLMAIFSGLLFLTLPTGGVGGSFIAFYGVFLALFL
HHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHH
TAGLGSGSTFQMISVIFRKLTMDRVKAEGGSDERAMREAATDTAAALGFISAIGAIGGFF
HHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IPKAFGSSLALTGSPVGAMKVFLIFYIACVVITWAVYGRHSKNKK
HHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SHSSAPERATGAVITEWRPEDPAFWQQRGHRVASRNLWISVPCLLLAFCVWMLFSAVAV
CCCCCCCCCCCCEEECCCCCCCHHHHHHCCCHHHCCCEEEHHHHHHHHHHHHHHHHHHH
NLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPLFGGRRWTAFSTGILIVPCVWLG
CCCCCCCCCCHHHHHHHHHCCCCCCHHHHCCHHHHHHHCCCCEEEECCCCHHHHHHHHHH
FAVQDTSTPFSTFIIISLLCGFAGANFASSMANISFFFPKQKQGGALGLNGGLGNMGVSV
HEECCCCCCHHHHHHHHHHHHHCCCHHHHHHCCEEEECCCCCCCCEEEECCCCCHHHHHH
MQLVAPLVVSLSIFAAFGSHGVEQPDGSQLYLANAAWIWVPFLAIFTLAAWFGMNELATS
HHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHCHHHHHHH
KASLKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAGFAMLSKTQFPDVQILHYAFFGPFI
HHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
GALARSAGGAISDRLGGTRVTLINFVLMAIFSGLLFLTLPTGGVGGSFIAFYGVFLALFL
HHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHH
TAGLGSGSTFQMISVIFRKLTMDRVKAEGGSDERAMREAATDTAAALGFISAIGAIGGFF
HHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IPKAFGSSLALTGSPVGAMKVFLIFYIACVVITWAVYGRHSKNKK
HHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2668029; 8905232; 9278503; 2657652 [H]