BacMap

Definition	Salmonella enterica subsp. enterica serovar Typhi str. Ty2 chromosome, complete genome.
Accession	NC_004631
Length	4,791,961

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The map label for this gene is ytbE [H]

Identifier: 29142020

GI number: 29142020

Start: 1639479

End: 1640348

Strand: Reverse

Name: ytbE [H]

Synonym: t1580

Alternate gene names: 29142020

Gene position: 1640348-1639479 (Counterclockwise)

Preceding gene: 29142021

Following gene: 29142018

Centisome position: 34.23

GC content: 46.67

Gene sequence:

>870_bases
GTGGAATATTCAATATTAAGTAACAACCTTAAAATGCCTATGGTGGGTTTTGGCGTTTTTAAGGTAACCGACAAAGAAGA
ATGTCAGCAGTCAGTGTTAAGCGCTATTCGCAGCGGTTATCGTCTTATCGATACCGCTGCGGTATACGGCAATGAAGATG
CCGTTGGTGACGCTGTTCGTGAAGCCATCGCGACAGGTCTGTGTACCCGTGAGGAGTTATTTATTACTTCTAAACTGTGG
GTGCAAGATATGGCTAACTACGATCTGGCCAAAGCAGGTATTGAAGCGTCGCTGAAAAAATCCGGGTTAGATTATTTTGA
TCTCTATTTATTGCATCAGGCAATGGGCGATTATTTTAGCGCGTGGCGCGCGCTGGAAGACGCTTATGAAGCCGGTAAAC
TAAAAGCCATTGGCGTGTCCAACTTCTATGCGCATGTGTTAGCCAACTTCTGTGAAACCGTCAGAATTACGCCAATGGTA
AACCAGGTCGAATTGCATCCATACTTTGCACAACCCGCCGCGCTGGAAACCATGAAGCATTATAACGTTCAGCCTGAAGC
GTGGGCACCGTTAGGCGGCGGGAGACATAAACCGTATGAAAATGTCATGCTTCAAAGGATTGCCGATGCCCATCAAAAAA
CCATCGCGCAGGTTGTCCTGCGTTGGAACGTGCAGCGTGGCGTCACGGTTATTCCTAAATCCACTCGACAGGAACGTATT
GAAGAGAACTTTGCCATCTGGGATTTCTCACTAACGGATAACGAAATGGCTCAAATCAACGCCCTTGATTTAGGCTACGT
TGGCGAAGCGGTGAAGCATTTTAATCCTGAATTTGTTCGCGGTTGTCTTGGCGTTAAAATCCACGATTGA

Upstream 100 bases:

>100_bases
TAATGAACTTTTTGTATAAATATAATCGGATTTAACAATACTCATTGTACCTGTATTTTGCTCTTATCATGGCGTCTTTA
CGACGAAAGGGTGCTTTAGC

Downstream 100 bases:

>100_bases
TCCCGGCGCTTGCGGATGTTTTCTCGCATGAACAGCTTCTAACACGGTTGCCGGAGCGTAGACGGCAACCGTGTGATATT
CGCCTGGCCGTCAGGCGCCG

Product: oxidoreductase

Products: 2-keto-L-gulonate; NADP [C]

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MEYSILSNNLKMPMVGFGVFKVTDKEECQQSVLSAIRSGYRLIDTAAVYGNEDAVGDAVREAIATGLCTREELFITSKLW
VQDMANYDLAKAGIEASLKKSGLDYFDLYLLHQAMGDYFSAWRALEDAYEAGKLKAIGVSNFYAHVLANFCETVRITPMV
NQVELHPYFAQPAALETMKHYNVQPEAWAPLGGGRHKPYENVMLQRIADAHQKTIAQVVLRWNVQRGVTVIPKSTRQERI
EENFAIWDFSLTDNEMAQINALDLGYVGEAVKHFNPEFVRGCLGVKIHD

Sequences:

>Translated_289_residues
MEYSILSNNLKMPMVGFGVFKVTDKEECQQSVLSAIRSGYRLIDTAAVYGNEDAVGDAVREAIATGLCTREELFITSKLW
VQDMANYDLAKAGIEASLKKSGLDYFDLYLLHQAMGDYFSAWRALEDAYEAGKLKAIGVSNFYAHVLANFCETVRITPMV
NQVELHPYFAQPAALETMKHYNVQPEAWAPLGGGRHKPYENVMLQRIADAHQKTIAQVVLRWNVQRGVTVIPKSTRQERI
EENFAIWDFSLTDNEMAQINALDLGYVGEAVKHFNPEFVRGCLGVKIHD
>Mature_289_residues
MEYSILSNNLKMPMVGFGVFKVTDKEECQQSVLSAIRSGYRLIDTAAVYGNEDAVGDAVREAIATGLCTREELFITSKLW
VQDMANYDLAKAGIEASLKKSGLDYFDLYLLHQAMGDYFSAWRALEDAYEAGKLKAIGVSNFYAHVLANFCETVRITPMV
NQVELHPYFAQPAALETMKHYNVQPEAWAPLGGGRHKPYENVMLQRIADAHQKTIAQVVLRWNVQRGVTVIPKSTRQERI
EENFAIWDFSLTDNEMAQINALDLGYVGEAVKHFNPEFVRGCLGVKIHD

Specific function: Reduction [C]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI5174695, Length=293, Percent_Identity=38.5665529010239, Blast_Score=189, Evalue=2e-48,
Organism=Homo sapiens, GI310109922, Length=265, Percent_Identity=36.9811320754717, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI223468663, Length=285, Percent_Identity=36.4912280701754, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI300116271, Length=270, Percent_Identity=38.1481481481481, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI300116273, Length=276, Percent_Identity=36.9565217391304, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI45446745, Length=286, Percent_Identity=35.3146853146853, Blast_Score=168, Evalue=4e-42,
Organism=Homo sapiens, GI4503285, Length=286, Percent_Identity=35.3146853146853, Blast_Score=168, Evalue=4e-42,
Organism=Homo sapiens, GI310109920, Length=286, Percent_Identity=34.965034965035, Blast_Score=168, Evalue=4e-42,
Organism=Homo sapiens, GI24497583, Length=286, Percent_Identity=34.965034965035, Blast_Score=167, Evalue=8e-42,
Organism=Homo sapiens, GI5453543, Length=286, Percent_Identity=34.965034965035, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI24497577, Length=289, Percent_Identity=34.6020761245675, Blast_Score=165, Evalue=4e-41,
Organism=Homo sapiens, GI5174391, Length=289, Percent_Identity=34.6020761245675, Blast_Score=165, Evalue=4e-41,
Organism=Homo sapiens, GI24497585, Length=289, Percent_Identity=34.9480968858132, Blast_Score=163, Evalue=1e-40,
Organism=Homo sapiens, GI291291012, Length=280, Percent_Identity=33.9285714285714, Blast_Score=151, Evalue=6e-37,
Organism=Homo sapiens, GI93277124, Length=296, Percent_Identity=32.4324324324324, Blast_Score=150, Evalue=9e-37,
Organism=Homo sapiens, GI4502049, Length=287, Percent_Identity=35.191637630662, Blast_Score=142, Evalue=3e-34,
Organism=Homo sapiens, GI310109924, Length=150, Percent_Identity=35.3333333333333, Blast_Score=95, Evalue=8e-20,
Organism=Homo sapiens, GI207028673, Length=103, Percent_Identity=43.6893203883495, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI310109926, Length=103, Percent_Identity=42.7184466019417, Blast_Score=85, Evalue=8e-17,
Organism=Escherichia coli, GI87082198, Length=257, Percent_Identity=40.0778210116732, Blast_Score=196, Evalue=1e-51,
Organism=Escherichia coli, GI1786400, Length=259, Percent_Identity=33.2046332046332, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1788081, Length=276, Percent_Identity=30.0724637681159, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI48994888, Length=282, Percent_Identity=25.886524822695, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17550248, Length=283, Percent_Identity=36.7491166077738, Blast_Score=175, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17564128, Length=306, Percent_Identity=33.6601307189542, Blast_Score=168, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17566692, Length=288, Percent_Identity=36.1111111111111, Blast_Score=163, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17537077, Length=288, Percent_Identity=34.375, Blast_Score=159, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI71998625, Length=269, Percent_Identity=34.5724907063197, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17537075, Length=291, Percent_Identity=33.6769759450172, Blast_Score=155, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17561298, Length=274, Percent_Identity=31.7518248175182, Blast_Score=150, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI17552492, Length=268, Percent_Identity=28.7313432835821, Blast_Score=131, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17537079, Length=296, Percent_Identity=27.7027027027027, Blast_Score=127, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17538386, Length=272, Percent_Identity=28.6764705882353, Blast_Score=126, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17562292, Length=275, Percent_Identity=26.9090909090909, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17561300, Length=272, Percent_Identity=28.3088235294118, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17550246, Length=74, Percent_Identity=45.945945945946, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6321896, Length=303, Percent_Identity=34.6534653465347, Blast_Score=182, Evalue=4e-47,
Organism=Saccharomyces cerevisiae, GI6324694, Length=301, Percent_Identity=30.8970099667774, Blast_Score=149, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6320576, Length=288, Percent_Identity=30.5555555555556, Blast_Score=148, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6322556, Length=267, Percent_Identity=33.7078651685393, Blast_Score=143, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6319625, Length=317, Percent_Identity=29.6529968454259, Blast_Score=136, Evalue=5e-33,
Organism=Saccharomyces cerevisiae, GI6320079, Length=289, Percent_Identity=28.3737024221453, Blast_Score=112, Evalue=9e-26,
Organism=Drosophila melanogaster, GI24662789, Length=292, Percent_Identity=36.6438356164384, Blast_Score=180, Evalue=8e-46,
Organism=Drosophila melanogaster, GI24657054, Length=290, Percent_Identity=37.2413793103448, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24644950, Length=299, Percent_Identity=33.4448160535117, Blast_Score=164, Evalue=5e-41,
Organism=Drosophila melanogaster, GI24663317, Length=302, Percent_Identity=35.7615894039735, Blast_Score=164, Evalue=6e-41,
Organism=Drosophila melanogaster, GI20129731, Length=287, Percent_Identity=36.2369337979094, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI21356425, Length=289, Percent_Identity=34.2560553633218, Blast_Score=160, Evalue=7e-40,
Organism=Drosophila melanogaster, GI221378297, Length=319, Percent_Identity=31.3479623824451, Blast_Score=157, Evalue=7e-39,
Organism=Drosophila melanogaster, GI24662785, Length=272, Percent_Identity=38.2352941176471, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24662781, Length=277, Percent_Identity=37.5451263537906, Blast_Score=155, Evalue=2e-38,
Organism=Drosophila melanogaster, GI45553081, Length=288, Percent_Identity=31.5972222222222, Blast_Score=154, Evalue=7e-38,
Organism=Drosophila melanogaster, GI281366140, Length=255, Percent_Identity=31.7647058823529, Blast_Score=134, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: NA

Molecular weight: Translated: 32506; Mature: 32506

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00063 ALDOKETO_REDUCTASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYSILSNNLKMPMVGFGVFKVTDKEECQQSVLSAIRSGYRLIDTAAVYGNEDAVGDAVR
CCCCEECCCCCCCEEECEEEEECCHHHHHHHHHHHHHCCCEEEHHHHHCCCCHHHHHHHH
EAIATGLCTREELFITSKLWVQDMANYDLAKAGIEASLKKSGLDYFDLYLLHQAMGDYFS
HHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
AWRALEDAYEAGKLKAIGVSNFYAHVLANFCETVRITPMVNQVELHPYFAQPAALETMKH
HHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHEEECCCCCEEEECCCCCCHHHHHHHHH
YNVQPEAWAPLGGGRHKPYENVMLQRIADAHQKTIAQVVLRWNVQRGVTVIPKSTRQERI
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHH
EENFAIWDFSLTDNEMAQINALDLGYVGEAVKHFNPEFVRGCLGVKIHD
HCCCEEEEEECCCCCHHEEECCCHHHHHHHHHHCCHHHHHHHHCCEECC
>Mature Secondary Structure
MEYSILSNNLKMPMVGFGVFKVTDKEECQQSVLSAIRSGYRLIDTAAVYGNEDAVGDAVR
CCCCEECCCCCCCEEECEEEEECCHHHHHHHHHHHHHCCCEEEHHHHHCCCCHHHHHHHH
EAIATGLCTREELFITSKLWVQDMANYDLAKAGIEASLKKSGLDYFDLYLLHQAMGDYFS
HHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
AWRALEDAYEAGKLKAIGVSNFYAHVLANFCETVRITPMVNQVELHPYFAQPAALETMKH
HHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHEEECCCCCEEEECCCCCCHHHHHHHHH
YNVQPEAWAPLGGGRHKPYENVMLQRIADAHQKTIAQVVLRWNVQRGVTVIPKSTRQERI
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHH
EENFAIWDFSLTDNEMAQINALDLGYVGEAVKHFNPEFVRGCLGVKIHD
HCCCEEEEEECCCCCHHEEECCCHHHHHHHHHHCCHHHHHHHHCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2,5-diketo-D-gluconate; NADPH [C]

Specific reaction: 2,5-diketo-D-gluconate + NADPH = 2-keto-L-gulonate + NADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]