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The map label for this gene is pntA [H]

Identifier: 29141852

GI number: 29141852

Start: 1443419

End: 1444948

Strand: Direct

Name: pntA [H]

Synonym: t1399

Alternate gene names: 29141852

Gene position: 1443419-1444948 (Clockwise)

Preceding gene: 29141850

Following gene: 29141853

Centisome position: 30.12

GC content: 55.03

Gene sequence:

>1530_bases
ATGCGAATTGGCATACCAAAAGAGCGGTTACCCAATGAAACCCGTGTCGCTGCGACGCCGAAAACGGTAGAGCAGCTACT
GAAGCTGGGGTTTAGCGTCGCTATCGAAAGCGGGGCGGGTCAACTGGCAAGTTTTGACGACAAAGCGTTTGCGCAGGCTG
GCGCGGATATTGTAGACGGCAATGCTATCTGGCAATCGGAAATTATTCTCAAGGTTAATGCGCCTGAAGAAGAGGAAATC
GCTTTACTGAACCCCGGGACTACGCTGGTCAGTTTTATCTGGCCTGCGCAAAATCCTGGGTTAATGGAGAAACTTGCCGA
ACGTAAGGTTACGGTGATGGCGATGGACTCCGTGCCGCGTATTTCCCGCGCGCAGTCGCTCGACGCGTTAAGCTCAATGG
CGAATATCGCGGGTTATCGCGCCATTGTAGAGGCCGCGCATGAATTTGGGCGCTTCTTTACCGGGCAAATCACTGCCGCC
GGGAAAGTGCCGCCGGCGAAGGTGATGGTGATTGGCGCGGGCGTTGCCGGTCTTGCGGCTATCGGCGCGGCAAACAGCCT
GGGGGCGATTGTCCGCGCGTTTGACACTCGTCCTGAAGTCAAAGAGCAGGTCCAGAGTATGGGGGCTGAGTTCCTCGAAC
TGGATTTTAAAGAGGAAGCCGGTAGCGGCGATGGTTACGCCAAAGTGATGTCCGAGGCGTTTATCAAAGCGGAAATGGCG
CTCTTTGCCGCCCAGGCCAAAGAAGTCGATATCATTGTTACCACCGCGTTGATTCCGGGCAAACCTGCGCCGAAGCTGAT
TACCCGCGATATGGTCGATTCTATGAAGGCCGGCAGCGTGATTGTCGACCTGGCCGCGCAGAACGGCGGTAACTGCGAAT
ATACCGTCGCCAATCAGGTTGTGACGACGGACAATGGCGTAAAAGTGATTGGCTACACCGATCTACCGGGGCGTTTGCCG
ACGCAGTCATCCCAGCTTTATGGCACCAACCTGGTTAACCTGCTGAAGCTGCTGTGCAAAGAGAAAGACGGCAATATTGA
CGTGGATTTTGACGATGTCGTGATCCGTGGCGTGACGGTGATTCGTGACGGCGACATTACCTGGCCTGCGCCGCCAATCC
AGGTTTCCGCGCAGCCACAGGCCGCGCCTAAAGCAGCACCTGCGCCAAAAGAGCCGGAAAAGCCCGCTTCCCCCTGGCGG
AAGTATGCGTTAATGGCGCTGGCGATCATTCTTTTTGGCTGGCTGGCCGATGTCGCGCCAAAAGAGTTCCTCGGCCATTT
TACCGTGTTTGCGCTCGCCTGCGTGGTTGGTTATTACGTGGTGTGGAACGTGTCTCATGCGCTTCATACGCCATTAATGT
CGGTGACCAACGCAATTTCAGGGATTATTGTTGTCGGGGCATTATTGCAAATTGGTCAGGGCGGCTGGGTCAGCTTCCTG
AGCTTTATCGCGGTGCTTATCGCCAGCATTAATATTTTCGGTGGCTTCACCGTGACTCAGCGTATGCTGAAAATGTTCCG
GAAAAACTAA

Upstream 100 bases:

>100_bases
TATTCTGCTGGGGTAACATTTACCTCAAACGCGGGCAGGCGTACGCTTCTCGCGCTTACTGACAAACCATCATCAAAAAT
ACCGATGGAAGGGAACATTT

Downstream 100 bases:

>100_bases
GGGGTAACACATGTCTGGAGGATTAGTTACAGCTGCATACATTGTTGCCGCGATCCTGTTTATTTTCAGTCTTGCCGGGC
TTTCAAAGCATGAAACGTCC

Product: NAD(P) transhydrogenase subunit alpha

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit alpha; Pyridine nucleotide transhydrogenase subunit alpha [H]

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MRIGIPKERLPNETRVAATPKTVEQLLKLGFSVAIESGAGQLASFDDKAFAQAGADIVDGNAIWQSEIILKVNAPEEEEI
ALLNPGTTLVSFIWPAQNPGLMEKLAERKVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKEEAGSGDGYAKVMSEAFIKAEMA
LFAAQAKEVDIIVTTALIPGKPAPKLITRDMVDSMKAGSVIVDLAAQNGGNCEYTVANQVVTTDNGVKVIGYTDLPGRLP
TQSSQLYGTNLVNLLKLLCKEKDGNIDVDFDDVVIRGVTVIRDGDITWPAPPIQVSAQPQAAPKAAPAPKEPEKPASPWR
KYALMALAIILFGWLADVAPKEFLGHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGQGGWVSFL
SFIAVLIASINIFGGFTVTQRMLKMFRKN

Sequences:

>Translated_509_residues
MRIGIPKERLPNETRVAATPKTVEQLLKLGFSVAIESGAGQLASFDDKAFAQAGADIVDGNAIWQSEIILKVNAPEEEEI
ALLNPGTTLVSFIWPAQNPGLMEKLAERKVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKEEAGSGDGYAKVMSEAFIKAEMA
LFAAQAKEVDIIVTTALIPGKPAPKLITRDMVDSMKAGSVIVDLAAQNGGNCEYTVANQVVTTDNGVKVIGYTDLPGRLP
TQSSQLYGTNLVNLLKLLCKEKDGNIDVDFDDVVIRGVTVIRDGDITWPAPPIQVSAQPQAAPKAAPAPKEPEKPASPWR
KYALMALAIILFGWLADVAPKEFLGHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGQGGWVSFL
SFIAVLIASINIFGGFTVTQRMLKMFRKN
>Mature_509_residues
MRIGIPKERLPNETRVAATPKTVEQLLKLGFSVAIESGAGQLASFDDKAFAQAGADIVDGNAIWQSEIILKVNAPEEEEI
ALLNPGTTLVSFIWPAQNPGLMEKLAERKVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKEEAGSGDGYAKVMSEAFIKAEMA
LFAAQAKEVDIIVTTALIPGKPAPKLITRDMVDSMKAGSVIVDLAAQNGGNCEYTVANQVVTTDNGVKVIGYTDLPGRLP
TQSSQLYGTNLVNLLKLLCKEKDGNIDVDFDDVVIRGVTVIRDGDITWPAPPIQVSAQPQAAPKAAPAPKEPEKPASPWR
KYALMALAIILFGWLADVAPKEFLGHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGQGGWVSFL
SFIAVLIASINIFGGFTVTQRMLKMFRKN

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG3288

COG function: function code C; NAD/NADP transhydrogenase alpha subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=538, Percent_Identity=50.5576208178439, Blast_Score=480, Evalue=1e-135,
Organism=Homo sapiens, GI122939153, Length=538, Percent_Identity=50.5576208178439, Blast_Score=480, Evalue=1e-135,
Organism=Escherichia coli, GI1787887, Length=510, Percent_Identity=92.7450980392157, Blast_Score=937, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17550456, Length=519, Percent_Identity=51.252408477842, Blast_Score=483, Evalue=1e-137,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR004571 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 54237; Mature: 54237

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS00836 ALADH_PNT_1 ; PS00837 ALADH_PNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGIPKERLPNETRVAATPKTVEQLLKLGFSVAIESGAGQLASFDDKAFAQAGADIVDG
CCCCCCHHHCCCCCEEEECHHHHHHHHHCCHHEEEECCCCCCCCCCHHHHHHCCCCEECC
NAIWQSEIILKVNAPEEEEIALLNPGTTLVSFIWPAQNPGLMEKLAERKVTVMAMDSVPR
CEEEECEEEEEECCCCCCCEEEECCCCEEEEEECCCCCCCHHHHHHHCCEEEEEECCCCC
ISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAAGKVPPAKVMVIGAGVAGLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECEEEECCCCCCCEEEEEECCHHHHHH
IGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKEEAGSGDGYAKVMSEAFIKAEMA
HCCCHHHHHHHHHHCCCHHHHHHHHHCCCHHHEEECHHCCCCCCHHHHHHHHHHHHHHHH
LFAAQAKEVDIIVTTALIPGKPAPKLITRDMVDSMKAGSVIVDLAAQNGGNCEYTVANQV
HHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEECEE
VTTDNGVKVIGYTDLPGRLPTQSSQLYGTNLVNLLKLLCKEKDGNIDVDFDDVVIRGVTV
EECCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHEEEEEE
IRDGDITWPAPPIQVSAQPQAAPKAAPAPKEPEKPASPWRKYALMALAIILFGWLADVAP
EECCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCH
KEFLGHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGQGGWVSFL
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
SFIAVLIASINIFGGFTVTQRMLKMFRKN
HHHHHHHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MRIGIPKERLPNETRVAATPKTVEQLLKLGFSVAIESGAGQLASFDDKAFAQAGADIVDG
CCCCCCHHHCCCCCEEEECHHHHHHHHHCCHHEEEECCCCCCCCCCHHHHHHCCCCEECC
NAIWQSEIILKVNAPEEEEIALLNPGTTLVSFIWPAQNPGLMEKLAERKVTVMAMDSVPR
CEEEECEEEEEECCCCCCCEEEECCCCEEEEEECCCCCCCHHHHHHHCCEEEEEECCCCC
ISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAAGKVPPAKVMVIGAGVAGLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECEEEECCCCCCCEEEEEECCHHHHHH
IGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKEEAGSGDGYAKVMSEAFIKAEMA
HCCCHHHHHHHHHHCCCHHHHHHHHHCCCHHHEEECHHCCCCCCHHHHHHHHHHHHHHHH
LFAAQAKEVDIIVTTALIPGKPAPKLITRDMVDSMKAGSVIVDLAAQNGGNCEYTVANQV
HHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEECEE
VTTDNGVKVIGYTDLPGRLPTQSSQLYGTNLVNLLKLLCKEKDGNIDVDFDDVVIRGVTV
EECCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHEEEEEE
IRDGDITWPAPPIQVSAQPQAAPKAAPAPKEPEKPASPWRKYALMALAIILFGWLADVAP
EECCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCH
KEFLGHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGQGGWVSFL
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
SFIAVLIASINIFGGFTVTQRMLKMFRKN
HHHHHHHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3525165; 1633824; 9097039; 9278503; 1932078 [H]