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The map label for this gene is fruA [H]

Identifier: 29141161

GI number: 29141161

Start: 733087

End: 734775

Strand: Direct

Name: fruA [H]

Synonym: t0651

Alternate gene names: 29141161

Gene position: 733087-734775 (Clockwise)

Preceding gene: 29141160

Following gene: 29141164

Centisome position: 15.3

GC content: 59.03

Gene sequence:

>1689_bases
ATGAAAACGCTGCTGATAATTGACGCTAATCTCGGCCAGGCTCGCGCTTACATGGCGAAAACCCTGCTTGGAGCGGCGGC
GCATAAAGCAAATCTGGAAATTATCGACAATCCGAATGATGCTGAACTGGCGATCGTGCTGGGCGAATCTCTGCCGAACG
ACAATGCTTTGAACGGCAAAAAGGTTTGGCTGGGGGATATTGGTCGCGCCGTCGCGCATCCGGAACTGTTTCTGAGTGAA
GCCAAAAGCCATGCGACCCCTTACAGCGCCCCTGCTGCGGCTGCTCCTGCAGCGAGCGGCGGTCCGAAACGCGTGGTCGC
GGTAACGGCCTGCCCGACCGGCGTCGCCCATACTTTTATGGCGGCTGAAGCCATTGAAACAGAAGCGAAAAAACGCGGCT
GGTGGGTAAAAGTCGAAACGCGCGGCTCCGTGGGGGCCGGCAATGCCATCACCCCGGAAGAGGTGGCGGAAGCGGATTTA
GTGATTGTGGCGGCGGATATCGAAGTGGATCTGGCGAAGTTTGCCGGTCTCCCGATGTACCGCACGTCGACCGGCCTGGC
GCTGAAAAAGACGGCGCAGGAGCTGGATAAAGCCGTAGCGGAAGCGACGCCGTATCAACCGGCGGGTAAGGCATCGCAAG
CGGCGACCGAAGGGAAGAAAGAGAGCGCTGGCGCATACCGGCATCTGTTGACGGGCGTTTCTTACATGCTGCCTATGGTG
GTTGCCGGGGGACTGTGTATTGCGCTTTCCTTCGCCTTTGGTATTGAAGCCTTTAAGGTGCCGGACACGCTGGCGGCGGC
GCTGATGCAGATTGGCGGCGGTTCGGCGTTTGCGCTGATGGTGCCGGTACTGGCGGGTTATATCGCTTTCTCCATCGCGG
ATCGCCCTGGCCTTACGCCAGGTCTGATTGGCGGTATGCTGGCGGTTAGCACCGGTTCCGGTTTTATCGGCGGGATCATT
GCCGGCTTCCTTGCCGGGTATATGGCGAAGCTTATCAGTACCAAACTGAAACTTCCGCAAAGTATGGAAGCGCTGAAGCC
AATCCTGATCATCCCGTTAATTTCCAGTCTGGTGGTGGGGCTGGCGATGATTTACCTGATCGGTAAACCGGTTGCCGGGA
TTCTGGAAGGGCTCACCCACTGGCTGCAAACCATGGGGACGGCAAACGCGGTGCTGTTGGGCGCGATTCTCGGCGGGATG
ATGTGTACCGACATGGGCGGCCCGGTGAACAAAGCGGCGTATGCGTTTGGCGTTGGTCTGCTGAGTACGCAAACTTACGC
GCCGATGGCGGCGATCATGGCCGCAGGTATGGTACCGCCGTTAGCGCTGGGTCTGGCGACGATGGTGGCGCGTCGTAAGT
TCGACAAAGCGCAGCAGGAAGGCGGTAAAGCGGCGTTGGTGCTGGGGCTGTGCTTTATCACTGAAGGCGCTATTCCGTTT
GCGGCGCGCGACCCGATGCGTGTACTGCCGTGCTGTATCGTTGGCGGCGCGTTGACCGGGGCTATTTCTATGGCGGTAGG
CGCAAAATTGATGGCGCCGCATGGCGGTCTGTTTGTTCTGCTTATCCCAGGCGCAATTACGCCGGTATTGGGATACCTGC
TGGCAATTGTGGCCGGTACGCTGGTGGCAGGACTGGCTTATGCCGTCCTGAAACGTCCGGAGACGGAAGTCGCGGCAAAA
GCGGCATAA

Upstream 100 bases:

>100_bases
GTCAGCCAGAGCAATGTGGGTATTACCGATCGTCCTCAGTTGGCCGCAATGATGGCGCGTGTCGACTTACAACCGTTTAA
CTGACAGCAGGAGAGGCATA

Downstream 100 bases:

>100_bases
GAGAAACACGCTAAAAAAGGCAGAGCGCGCTCTGCCTTTTTTATTGCCTGAAGAACATTGAGCGTCAGGCCATCGCTTCG
GCAATTTGCTGGGCTTTTAA

Product: PTS system fructose-specific transporter subunits IIBC

Products: NA

Alternate protein names: EIIBC-Fru; Fructose-specific phosphotransferase enzyme IIB component; EIII-Fru; PTS system fructose-specific EIIB component; Fructose permease IIC component; PTS system fructose-specific EIIC component [H]

Number of amino acids: Translated: 562; Mature: 562

Protein sequence:

>562_residues
MKTLLIIDANLGQARAYMAKTLLGAAAHKANLEIIDNPNDAELAIVLGESLPNDNALNGKKVWLGDIGRAVAHPELFLSE
AKSHATPYSAPAAAAPAASGGPKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAEADL
VIVAADIEVDLAKFAGLPMYRTSTGLALKKTAQELDKAVAEATPYQPAGKASQAATEGKKESAGAYRHLLTGVSYMLPMV
VAGGLCIALSFAFGIEAFKVPDTLAAALMQIGGGSAFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGII
AGFLAGYMAKLISTKLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAVLLGAILGGM
MCTDMGGPVNKAAYAFGVGLLSTQTYAPMAAIMAAGMVPPLALGLATMVARRKFDKAQQEGGKAALVLGLCFITEGAIPF
AARDPMRVLPCCIVGGALTGAISMAVGAKLMAPHGGLFVLLIPGAITPVLGYLLAIVAGTLVAGLAYAVLKRPETEVAAK
AA

Sequences:

>Translated_562_residues
MKTLLIIDANLGQARAYMAKTLLGAAAHKANLEIIDNPNDAELAIVLGESLPNDNALNGKKVWLGDIGRAVAHPELFLSE
AKSHATPYSAPAAAAPAASGGPKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAEADL
VIVAADIEVDLAKFAGLPMYRTSTGLALKKTAQELDKAVAEATPYQPAGKASQAATEGKKESAGAYRHLLTGVSYMLPMV
VAGGLCIALSFAFGIEAFKVPDTLAAALMQIGGGSAFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGII
AGFLAGYMAKLISTKLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAVLLGAILGGM
MCTDMGGPVNKAAYAFGVGLLSTQTYAPMAAIMAAGMVPPLALGLATMVARRKFDKAQQEGGKAALVLGLCFITEGAIPF
AARDPMRVLPCCIVGGALTGAISMAVGAKLMAPHGGLFVLLIPGAITPVLGYLLAIVAGTLVAGLAYAVLKRPETEVAAK
AA
>Mature_562_residues
MKTLLIIDANLGQARAYMAKTLLGAAAHKANLEIIDNPNDAELAIVLGESLPNDNALNGKKVWLGDIGRAVAHPELFLSE
AKSHATPYSAPAAAAPAASGGPKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAEADL
VIVAADIEVDLAKFAGLPMYRTSTGLALKKTAQELDKAVAEATPYQPAGKASQAATEGKKESAGAYRHLLTGVSYMLPMV
VAGGLCIALSFAFGIEAFKVPDTLAAALMQIGGGSAFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGII
AGFLAGYMAKLISTKLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAVLLGAILGGM
MCTDMGGPVNKAAYAFGVGLLSTQTYAPMAAIMAAGMVPPLALGLATMVARRKFDKAQQEGGKAALVLGLCFITEGAIPF
AARDPMRVLPCCIVGGALTGAISMAVGAKLMAPHGGLFVLLIPGAITPVLGYLLAIVAGTLVAGLAYAVLKRPETEVAAK
AA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1299

COG function: function code G; Phosphotransferase system, fructose-specific IIC component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788492, Length=563, Percent_Identity=92.7175843694494, Blast_Score=1021, Evalue=0.0,
Organism=Escherichia coli, GI1786951, Length=509, Percent_Identity=34.9705304518664, Blast_Score=257, Evalue=1e-69,
Organism=Escherichia coli, GI87082348, Length=478, Percent_Identity=33.6820083682008, Blast_Score=237, Evalue=2e-63,
Organism=Escherichia coli, GI1790386, Length=301, Percent_Identity=43.8538205980066, Blast_Score=211, Evalue=1e-55,
Organism=Escherichia coli, GI1788729, Length=362, Percent_Identity=28.1767955801105, Blast_Score=128, Evalue=8e-31,
Organism=Escherichia coli, GI1790387, Length=88, Percent_Identity=52.2727272727273, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1788730, Length=103, Percent_Identity=38.8349514563107, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1790390, Length=65, Percent_Identity=44.6153846153846, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR003353
- InterPro:   IPR006327 [H]

Pfam domain/function: PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 57289; Mature: 57289

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: PS51099 PTS_EIIB_TYPE_2 ; PS51104 PTS_EIIC_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLLIIDANLGQARAYMAKTLLGAAAHKANLEIIDNPNDAELAIVLGESLPNDNALNGK
CCEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEEEECCCCCCCCCCCC
KVWLGDIGRAVAHPELFLSEAKSHATPYSAPAAAAPAASGGPKRVVAVTACPTGVAHTFM
EEEHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHH
AAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAEADLVIVAADIEVDLAKFAGLPMY
HHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHCCCCEEEEEECCEEEHHHHCCCCCE
RTSTGLALKKTAQELDKAVAEATPYQPAGKASQAATEGKKESAGAYRHLLTGVSYMLPMV
ECCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
VAGGLCIALSFAFGIEAFKVPDTLAAALMQIGGGSAFALMVPVLAGYIAFSIADRPGLTP
HHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCH
GLIGGMLAVSTGSGFIGGIIAGFLAGYMAKLISTKLKLPQSMEALKPILIIPLISSLVVG
HHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LAMIYLIGKPVAGILEGLTHWLQTMGTANAVLLGAILGGMMCTDMGGPVNKAAYAFGVGL
HHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
LSTQTYAPMAAIMAAGMVPPLALGLATMVARRKFDKAQQEGGKAALVLGLCFITEGAIPF
HCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHCCCCCC
AARDPMRVLPCCIVGGALTGAISMAVGAKLMAPHGGLFVLLIPGAITPVLGYLLAIVAGT
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCEEEEECCCHHHHHHHHHHHHHHHH
LVAGLAYAVLKRPETEVAAKAA
HHHHHHHHHHCCCCHHHHCCCC
>Mature Secondary Structure
MKTLLIIDANLGQARAYMAKTLLGAAAHKANLEIIDNPNDAELAIVLGESLPNDNALNGK
CCEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEEEECCCCCCCCCCCC
KVWLGDIGRAVAHPELFLSEAKSHATPYSAPAAAAPAASGGPKRVVAVTACPTGVAHTFM
EEEHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHH
AAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAEADLVIVAADIEVDLAKFAGLPMY
HHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHCCCCEEEEEECCEEEHHHHCCCCCE
RTSTGLALKKTAQELDKAVAEATPYQPAGKASQAATEGKKESAGAYRHLLTGVSYMLPMV
ECCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
VAGGLCIALSFAFGIEAFKVPDTLAAALMQIGGGSAFALMVPVLAGYIAFSIADRPGLTP
HHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCH
GLIGGMLAVSTGSGFIGGIIAGFLAGYMAKLISTKLKLPQSMEALKPILIIPLISSLVVG
HHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LAMIYLIGKPVAGILEGLTHWLQTMGTANAVLLGAILGGMMCTDMGGPVNKAAYAFGVGL
HHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
LSTQTYAPMAAIMAAGMVPPLALGLATMVARRKFDKAQQEGGKAALVLGLCFITEGAIPF
HCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHCCCCCC
AARDPMRVLPCCIVGGALTGAISMAVGAKLMAPHGGLFVLLIPGAITPVLGYLLAIVAGT
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCEEEEECCCHHHHHHHHHHHHHHHH
LVAGLAYAVLKRPETEVAAKAA
HHHHHHHHHHCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3076173; 9278503; 9097040; 1981619 [H]