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The map label for this gene is yfbB [H]

Identifier: 29141070

GI number: 29141070

Start: 631492

End: 632250

Strand: Direct

Name: yfbB [H]

Synonym: t0555

Alternate gene names: 29141070

Gene position: 631492-632250 (Clockwise)

Preceding gene: 29141069

Following gene: 29141071

Centisome position: 13.18

GC content: 58.76

Gene sequence:

>759_bases
ATGATGTTGCACGCGCAGCACATGCCCGGACAGCCTGGGACGCCCTCGCTGGTTTTCCTGCACGGTTTTTCTGGCGATTG
CCGCGAATGGCAGCCGGTTGGCGAACAGTTTCACGGCTGTTCGCGGCTTTATATTGACTTACCAGGACATGGCGGATCTG
CGGCTATTCCGGTCGGCGGCTTTGCCGATGTCATCAGATTACTGCGCGCCACGTTAATTAGTTACAACATACTAAAGTTT
TGGTTGGTGGGATACTCCCTTGGCGGCCGTGTGGCGATGATGGCAGCCTGCCAGGGAATACCCGGCCTGTGCGGCCTGGT
GGTCGAAGGCGGTCATCCGGGGCTGCAAAATGAACAGGCGCGCGCTGAAAGACGATTGTCCGACGGGCGCTGGGCAGAGC
GTTTTCGTCATGAACCCTTGACTGAGGTTTTTCACGACTGGTATCAGCAGCCGGTGTTCGCCTCGCTGACGGCGCAGCAA
AGGCAGGCGCTAACGGCGCTGCGTAGCCAGAATAACGGCGAAACGCTGGCTGCGATGCTGGAGGCGACATCGCTTGCCGT
CCAGCCGGATTTACGCGAGGTGCTTAACGCGCTGGCATTTCCGTTTTATTATTTATGTGGCGAACGCGACAGCAAATTCC
GCGCCCTGGCGCAGGAAGTGGCGGCGACATGCCATGTCATTCGTAACGCCGGACACAACGCGCACCGGGAAAACCCCGCT
GGCGTGGTAGACAGTCTGGCTCAGATTCTGCGTCTCTGA

Upstream 100 bases:

>100_bases
GCGCGTGGCGAACCCCGGCGACAACGGTGATTGAGCTGGTGGTAAACGATACGGATGGCGCGCAGACGCTGCAGCAACTT
TTGGCTCAGGTAAGCCATCT

Downstream 100 bases:

>100_bases
CTAAAGGACACACTATGATCTATCCTGATGAAACAATGCTTTACGCACCGGTAGAATGGCACGACTGCTCGGAGGGTTAC
ACCGACATTCGTTATGAAAA

Product: acyl-CoA thioester hydrolase YfbB

Products: NA

Alternate protein names: SHCHC synthase [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MMLHAQHMPGQPGTPSLVFLHGFSGDCREWQPVGEQFHGCSRLYIDLPGHGGSAAIPVGGFADVIRLLRATLISYNILKF
WLVGYSLGGRVAMMAACQGIPGLCGLVVEGGHPGLQNEQARAERRLSDGRWAERFRHEPLTEVFHDWYQQPVFASLTAQQ
RQALTALRSQNNGETLAAMLEATSLAVQPDLREVLNALAFPFYYLCGERDSKFRALAQEVAATCHVIRNAGHNAHRENPA
GVVDSLAQILRL

Sequences:

>Translated_252_residues
MMLHAQHMPGQPGTPSLVFLHGFSGDCREWQPVGEQFHGCSRLYIDLPGHGGSAAIPVGGFADVIRLLRATLISYNILKF
WLVGYSLGGRVAMMAACQGIPGLCGLVVEGGHPGLQNEQARAERRLSDGRWAERFRHEPLTEVFHDWYQQPVFASLTAQQ
RQALTALRSQNNGETLAAMLEATSLAVQPDLREVLNALAFPFYYLCGERDSKFRALAQEVAATCHVIRNAGHNAHRENPA
GVVDSLAQILRL
>Mature_252_residues
MMLHAQHMPGQPGTPSLVFLHGFSGDCREWQPVGEQFHGCSRLYIDLPGHGGSAAIPVGGFADVIRLLRATLISYNILKF
WLVGYSLGGRVAMMAACQGIPGLCGLVVEGGHPGLQNEQARAERRLSDGRWAERFRHEPLTEVFHDWYQQPVFASLTAQQ
RQALTALRSQNNGETLAAMLEATSLAVQPDLREVLNALAFPFYYLCGERDSKFRALAQEVAATCHVIRNAGHNAHRENPA
GVVDSLAQILRL

Specific function: Catalyzes a proton abstraction reaction that results in 2,5-elimination of pyruvate from 2-succinyl-5-enolpyruvyl-6- hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) and the formation of 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate (SHCHC). Is also

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. MenH family [H]

Homologues:

Organism=Escherichia coli, GI1788598, Length=252, Percent_Identity=75.7936507936508, Blast_Score=389, Evalue=1e-109,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR022485 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =4.2.99.20 [H]

Molecular weight: Translated: 27681; Mature: 27681

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMLHAQHMPGQPGTPSLVFLHGFSGDCREWQPVGEQFHGCSRLYIDLPGHGGSAAIPVGG
CCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCC
FADVIRLLRATLISYNILKFWLVGYSLGGRVAMMAACQGIPGLCGLVVEGGHPGLQNEQA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHEEEECCCCCCCCHHH
RAERRLSDGRWAERFRHEPLTEVFHDWYQQPVFASLTAQQRQALTALRSQNNGETLAAML
HHHHHCCCCHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
EATSLAVQPDLREVLNALAFPFYYLCGERDSKFRALAQEVAATCHVIRNAGHNAHRENPA
HHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
GVVDSLAQILRL
HHHHHHHHHHCC
>Mature Secondary Structure
MMLHAQHMPGQPGTPSLVFLHGFSGDCREWQPVGEQFHGCSRLYIDLPGHGGSAAIPVGG
CCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCC
FADVIRLLRATLISYNILKFWLVGYSLGGRVAMMAACQGIPGLCGLVVEGGHPGLQNEQA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHEEEECCCCCCCCHHH
RAERRLSDGRWAERFRHEPLTEVFHDWYQQPVFASLTAQQRQALTALRSQNNGETLAAML
HHHHHCCCCHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
EATSLAVQPDLREVLNALAFPFYYLCGERDSKFRALAQEVAATCHVIRNAGHNAHRENPA
HHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
GVVDSLAQILRL
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA