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The map label for this gene is nuoM [H]

Identifier: 29141062

GI number: 29141062

Start: 622340

End: 623869

Strand: Direct

Name: nuoM [H]

Synonym: t0547

Alternate gene names: 29141062

Gene position: 622340-623869 (Clockwise)

Preceding gene: 29141061

Following gene: 161486812

Centisome position: 12.99

GC content: 55.69

Gene sequence:

>1530_bases
ATGTTATTACCCTGGTTGATATTAATCCCCTTTATCGGCGGCTTTCTGTGCTGGCAGACCGAACGCTTTGGCGTGAAAGT
GCCGCGCTGGATAGCGCTGATTACCATGGAACTGACGCTGGCGCTCGGTCTGCAATTGTGGTTGCAGGGCGGCTACTCAC
TGACGCAATCCGCCGGTATTCCACAGTGGCAATCTGAATTTGTCCTGCCGTGGATCCCGCGTTTTGGCATCTCTATCCAT
CTGGCGCTCGACGGACTGTCGCTGCTAATGGTGGTGCTGACCGGCCTGCTCGGCGTTCTGGCGGTGCTTTGCTCTTGGCG
AGAAATCGAAAAATATCAGGGCTTCTTCCACCTCAACCTGATGTGGATCTTAGGCGGCGTTATCGGCGTGTTCCTGGCCA
TCGACATGTTCCTGTTCTTCTTCTTCTGGGAAATGATGCTGGTGCCGATGTACTTCCTGATAGCGCTGTGGGGCCACAAA
GCTTCTGACGGTAAAACGCGTATCACGGCGGCAACCAAGTTCTTCATTTACACCCAGGCGAGTGGTCTGGTGATGTTGAT
TGCCATCCTGGCGCTGGTGTTTGTGCACTATAAAGCGACCAGCGTGTGGACCTTCAACTATGAAGAGCTGCTGAATACCC
CGATGTCCCACGGTGTGGAATATCTGCTGATGCTGGGCTTCTTCATCGCCTTCGCGGTGAAAATGCCGGTGGTTCCGCTG
CATGGCTGGCTGCCGGATGCGCACTCCCAGGCGCCGACCGCCGGTTCCGTCGACCTGGCGGGGATCTTGCTGAAAACCGC
CGCTTACGGTCTGCTGCGTTTCTCCCTGCCATTGTTCCCGAACGCGTCGGCGGAGTTTGCGCCCATCGCCATGTGGCTGG
GCGTCATCGGCATTTTCTACGGCGCGTGGATGGCCTTTACCCAATACGACATCAAGCGCCTGATTGCCTATACCTCCGTT
TCCCACATGGGCTTCGTGCTGATCGCTATCTACACCGGCAGTCAGCTGGCGTACCAGGGCGCGGTGATTCAGATGATTGC
ACACGGTTTGTCCGCAGCCGGTCTGTTCATTCTGTGTGGCCAGTTGTACGAACGTCTGCATACGCGTGACATGCGTATGA
TGGGCGGCCTGTGGGGCAAGATGAAGTGGTTGCCTGCGCTGTCCATGTTCTTTGCGGTGGCGACGCTCGGTATGCCGGGC
ACCGGTAACTTTGTCGGCGAATTTATGATTCTGTTCGGCAGCTACCAGGTGGTGCCGATGATTACCGTTATCTCGACCTT
TGGGCTGGTATTCGCCTCTGTATACTCGCTGGCGATGCTGCATCGCGCTTACTTTGGTAAAGCGAAGAGCCAGATTGCCA
GCCAGGAACTGCTAGGGATGTCGCTGCGCGAGCTGTTTATCATTCTGTTGCTGGTGGTGCTTCTGGTACTGCTTGGCTTC
TATCCGCAGCCGATTCTGGATACCTCGCATTCTGCGATGAGCAACATCCAGCAGTGGTTTGTTAATTCCGTTACTACTAC
AAGGCCGTAA

Upstream 100 bases:

>100_bases
GACGTTCAAAACGCGCTGCGTTTTGTGTGAGCACTGGCAGGATTTGGGCTGACGAGTGAGATAGCCTGAGCAGGAGTAAA
TATAAGGGACATACTTAGCT

Downstream 100 bases:

>100_bases
ATCGCCATGACAATAACTCCACAACACCTGATTGCGCTGCTACCGCTGCTGATCGTCGGCTTGACGGTGGTGGTTGTGAT
GCTCTCCATTGCGTGGCGAC

Product: NADH dehydrogenase subunit M

Products: NA

Alternate protein names: NADH dehydrogenase I subunit M; NDH-1 subunit M; NUO13 [H]

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMELTLALGLQLWLQGGYSLTQSAGIPQWQSEFVLPWIPRFGISIH
LALDGLSLLMVVLTGLLGVLAVLCSWREIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYKATSVWTFNYEELLNTPMSHGVEYLLMLGFFIAFAVKMPVVPL
HGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSLPLFPNASAEFAPIAMWLGVIGIFYGAWMAFTQYDIKRLIAYTSV
SHMGFVLIAIYTGSQLAYQGAVIQMIAHGLSAAGLFILCGQLYERLHTRDMRMMGGLWGKMKWLPALSMFFAVATLGMPG
TGNFVGEFMILFGSYQVVPMITVISTFGLVFASVYSLAMLHRAYFGKAKSQIASQELLGMSLRELFIILLLVVLLVLLGF
YPQPILDTSHSAMSNIQQWFVNSVTTTRP

Sequences:

>Translated_509_residues
MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMELTLALGLQLWLQGGYSLTQSAGIPQWQSEFVLPWIPRFGISIH
LALDGLSLLMVVLTGLLGVLAVLCSWREIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYKATSVWTFNYEELLNTPMSHGVEYLLMLGFFIAFAVKMPVVPL
HGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSLPLFPNASAEFAPIAMWLGVIGIFYGAWMAFTQYDIKRLIAYTSV
SHMGFVLIAIYTGSQLAYQGAVIQMIAHGLSAAGLFILCGQLYERLHTRDMRMMGGLWGKMKWLPALSMFFAVATLGMPG
TGNFVGEFMILFGSYQVVPMITVISTFGLVFASVYSLAMLHRAYFGKAKSQIASQELLGMSLRELFIILLLVVLLVLLGF
YPQPILDTSHSAMSNIQQWFVNSVTTTRP
>Mature_509_residues
MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMELTLALGLQLWLQGGYSLTQSAGIPQWQSEFVLPWIPRFGISIH
LALDGLSLLMVVLTGLLGVLAVLCSWREIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYKATSVWTFNYEELLNTPMSHGVEYLLMLGFFIAFAVKMPVVPL
HGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSLPLFPNASAEFAPIAMWLGVIGIFYGAWMAFTQYDIKRLIAYTSV
SHMGFVLIAIYTGSQLAYQGAVIQMIAHGLSAAGLFILCGQLYERLHTRDMRMMGGLWGKMKWLPALSMFFAVATLGMPG
TGNFVGEFMILFGSYQVVPMITVISTFGLVFASVYSLAMLHRAYFGKAKSQIASQELLGMSLRELFIILLLVVLLVLLGF
YPQPILDTSHSAMSNIQQWFVNSVTTTRP

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=338, Percent_Identity=32.8402366863905, Blast_Score=163, Evalue=5e-40,
Organism=Homo sapiens, GI251831117, Length=271, Percent_Identity=28.7822878228782, Blast_Score=66, Evalue=8e-11,
Organism=Escherichia coli, GI1788613, Length=509, Percent_Identity=96.0707269155206, Blast_Score=960, Evalue=0.0,
Organism=Escherichia coli, GI1788827, Length=349, Percent_Identity=26.3610315186246, Blast_Score=117, Evalue=3e-27,
Organism=Escherichia coli, GI1788831, Length=316, Percent_Identity=28.4810126582279, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1788614, Length=450, Percent_Identity=26, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI145693160, Length=439, Percent_Identity=24.8291571753986, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1788829, Length=197, Percent_Identity=29.4416243654822, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 56830; Mature: 56830

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMELTLALGLQLWLQGGYSLTQSAGI
CCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCC
PQWQSEFVLPWIPRFGISIHLALDGLSLLMVVLTGLLGVLAVLCSWREIEKYQGFFHLNL
CCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
MWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHKASDGKTRITAATKFFIYTQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEECC
SGLVMLIAILALVFVHYKATSVWTFNYEELLNTPMSHGVEYLLMLGFFIAFAVKMPVVPL
CHHHHHHHHHHHHHHHHHHCEEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEC
HGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSLPLFPNASAEFAPIAMWLGVIGIFY
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
GAWMAFTQYDIKRLIAYTSVSHMGFVLIAIYTGSQLAYQGAVIQMIAHGLSAAGLFILCG
HHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
QLYERLHTRDMRMMGGLWGKMKWLPALSMFFAVATLGMPGTGNFVGEFMILFGSYQVVPM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHH
ITVISTFGLVFASVYSLAMLHRAYFGKAKSQIASQELLGMSLRELFIILLLVVLLVLLGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
YPQPILDTSHSAMSNIQQWFVNSVTTTRP
CCCCCCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMELTLALGLQLWLQGGYSLTQSAGI
CCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCC
PQWQSEFVLPWIPRFGISIHLALDGLSLLMVVLTGLLGVLAVLCSWREIEKYQGFFHLNL
CCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
MWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHKASDGKTRITAATKFFIYTQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEECC
SGLVMLIAILALVFVHYKATSVWTFNYEELLNTPMSHGVEYLLMLGFFIAFAVKMPVVPL
CHHHHHHHHHHHHHHHHHHCEEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEC
HGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSLPLFPNASAEFAPIAMWLGVIGIFY
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
GAWMAFTQYDIKRLIAYTSVSHMGFVLIAIYTGSQLAYQGAVIQMIAHGLSAAGLFILCG
HHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
QLYERLHTRDMRMMGGLWGKMKWLPALSMFFAVATLGMPGTGNFVGEFMILFGSYQVVPM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHH
ITVISTFGLVFASVYSLAMLHRAYFGKAKSQIASQELLGMSLRELFIILLLVVLLVLLGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
YPQPILDTSHSAMSNIQQWFVNSVTTTRP
CCCCCCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]