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The map label for this gene is mraW [H]

Identifier: 29140665

GI number: 29140665

Start: 141813

End: 142754

Strand: Direct

Name: mraW [H]

Synonym: t0124

Alternate gene names: 29140665

Gene position: 141813-142754 (Clockwise)

Preceding gene: 29140664

Following gene: 29140666

Centisome position: 2.96

GC content: 56.26

Gene sequence:

>942_bases
ATGATGGAAAATTTTAAACACACTACGGTACTGTTGGATGAAGCCGTTAATGGGCTGAATATTCGTCCGGATGGTATCTA
TATTGATGGCACATTTGGTCGCGGCGGCCACTCGCGCCTGATCCTCTCGCAACTGGGCGAAGAGGGGCGCTTGCTGGCGA
TCGATCGCGATCCGCAGGCGATCGCCGTTGCGCAGACCATTAATGATCCTCGCTTCTCCATCATTCATGGACCTTTTTCC
GCGCTGGCTGATTATGTCGCCGAGCGCGAGCTTACCGGCAAGATTGACGGGATCCTTCTCGATCTTGGCGTCTCTTCTCC
GCAGCTTGACGATGCGGAGCGCGGTTTTTCATTTATGCGCGACGGTCCGCTGGATATGCGAATGGACCCGACGCGCGGCC
AGTCTGCCGCCGAGTGGTTACAAACGGCGGAGGAGGCGGATATCGCCTGGGTGCTGAAAACATTCGGCGAGGAGCGTTTT
GCCAAACGTATTGCTCACGCCATTGTTGAGCGCAATCGCGAACAGCCGATGACCCGCACCAAAGAGCTGGCGGAAGTGGT
TGCGGCGGCGACCCCGGTAAAAGACAAATTCAAACATCCCGCGACCCGTACCTTCCAGGCGGTGCGCATCTGGGTAAACA
GTGAACTGGAGGAGATAGAGCAGGCGCTAAAAAGCTCGCTCAGCGTACTGGCCTCAGGCGGGCGGCTTTCAATCATCAGT
TTCCACTCGCTGGAAGACCGTATTGTGAAACGCTTTATGCGTGAGCAAAGCCGCGGTCCGCAGGTACCGGCGGGATTACC
GATGACGGAAGCGCAGCTCAAAAAACTGGGCGGTCGTGAGTTACGAGCGTTAGGCAAGTTGATGCCGGGTGAAAAAGAGG
TAGCTGAAAATCCTCGGGCCCGTAGTTCAGTTCTGCGTATCGCAGAGAGGACGAACGCATGA

Upstream 100 bases:

>100_bases
GATGAAACGACCTGGTATCAACAGGTCAAGGAAGATATCGACGCTGAACAGTCAGCTACCGAAACGTTATCGGAGCGGCT
GCAGGACTTGTCTCTATAAG

Downstream 100 bases:

>100_bases
TCAGCAGAGTGACAGAAGCCCTAAGCAAAGTTAAGGGATCGATAGGAAGCAACGAGCGCCATGCCTTGCCTGGCGTGATC
GGTGACGATCTTTTGCGGTT

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MMENFKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQLGEEGRLLAIDRDPQAIAVAQTINDPRFSIIHGPFS
ALADYVAERELTGKIDGILLDLGVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTFGEERF
AKRIAHAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEEIEQALKSSLSVLASGGRLSIIS
FHSLEDRIVKRFMREQSRGPQVPAGLPMTEAQLKKLGGRELRALGKLMPGEKEVAENPRARSSVLRIAERTNA

Sequences:

>Translated_313_residues
MMENFKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQLGEEGRLLAIDRDPQAIAVAQTINDPRFSIIHGPFS
ALADYVAERELTGKIDGILLDLGVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTFGEERF
AKRIAHAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEEIEQALKSSLSVLASGGRLSIIS
FHSLEDRIVKRFMREQSRGPQVPAGLPMTEAQLKKLGGRELRALGKLMPGEKEVAENPRARSSVLRIAERTNA
>Mature_313_residues
MMENFKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQLGEEGRLLAIDRDPQAIAVAQTINDPRFSIIHGPFS
ALADYVAERELTGKIDGILLDLGVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTFGEERF
AKRIAHAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEEIEQALKSSLSVLASGGRLSIIS
FHSLEDRIVKRFMREQSRGPQVPAGLPMTEAQLKKLGGRELRALGKLMPGEKEVAENPRARSSVLRIAERTNA

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA [H]

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family [H]

Homologues:

Organism=Homo sapiens, GI165377209, Length=334, Percent_Identity=40.4191616766467, Blast_Score=204, Evalue=7e-53,
Organism=Homo sapiens, GI165377202, Length=189, Percent_Identity=42.8571428571429, Blast_Score=135, Evalue=4e-32,
Organism=Escherichia coli, GI1786270, Length=313, Percent_Identity=95.2076677316294, Blast_Score=607, Evalue=1e-175,
Organism=Drosophila melanogaster, GI62472493, Length=332, Percent_Identity=39.4578313253012, Blast_Score=196, Evalue=2e-50,
Organism=Drosophila melanogaster, GI28571637, Length=332, Percent_Identity=39.4578313253012, Blast_Score=196, Evalue=2e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002903 [H]

Pfam domain/function: PF01795 Methyltransf_5 [H]

EC number: NA

Molecular weight: Translated: 34747; Mature: 34747

Theoretical pI: Translated: 6.82; Mature: 6.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMENFKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQLGEEGRLLAIDRDPQA
CCCCCHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCHHHHHHHCCCCCCEEEEECCCCE
IAVAQTINDPRFSIIHGPFSALADYVAERELTGKIDGILLDLGVSSPQLDDAERGFSFMR
EEEEECCCCCCEEEEECCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHCHHHHC
DGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTFGEERFAKRIAHAIVERNREQPMTRT
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
KELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEEIEQALKSSLSVLASGGRLSIIS
HHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEE
FHSLEDRIVKRFMREQSRGPQVPAGLPMTEAQLKKLGGRELRALGKLMPGEKEVAENPRA
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCHHHHHHHHCCCCCHHHHCCCHH
RSSVLRIAERTNA
HHHHHHHHHHCCC
>Mature Secondary Structure
MMENFKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQLGEEGRLLAIDRDPQA
CCCCCHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCHHHHHHHCCCCCCEEEEECCCCE
IAVAQTINDPRFSIIHGPFSALADYVAERELTGKIDGILLDLGVSSPQLDDAERGFSFMR
EEEEECCCCCCEEEEECCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHCHHHHC
DGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTFGEERFAKRIAHAIVERNREQPMTRT
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
KELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEEIEQALKSSLSVLASGGRLSIIS
HHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEE
FHSLEDRIVKRFMREQSRGPQVPAGLPMTEAQLKKLGGRELRALGKLMPGEKEVAENPRA
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCHHHHHHHHCCCCCHHHHCCCHH
RSSVLRIAERTNA
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA