Click here to switch to the map view.

The map label for this gene is polB [H]

Identifier: 29140642

GI number: 29140642

Start: 112871

End: 115222

Strand: Reverse

Name: polB [H]

Synonym: t0100

Alternate gene names: 29140642

Gene position: 115222-112871 (Counterclockwise)

Preceding gene: 29140643

Following gene: 29140641

Centisome position: 2.4

GC content: 56.59

Gene sequence:

>2352_bases
GTGGCGCAGGCAGGTTTCATTTTAACCCGGCACTGGCGGGATACGCCGCAAGGGACGGAGGTCTCTTTCTGGCTGGCGAC
AGACAACGGCCCTTTGCAGGCGACGCTGGCGCCGCAAGAGTCCGTGGCGTTTATTCCGACCAGCCAGACCTCCCGCGCCG
CCTCGCTGCTACAGGCGGAAAAAGACTATCGCCTGACGCCGCTCCAGCTGCGAGACTTTCACCGTCAGCCGGTATCCGGT
CTGTATTGCCGCACTCACCGCCAGCTTATGCGGATGGAGAAACTGCTGCGCGAAAACGGCGTCACCGTTTATGAAGCGGA
TGTCCGCCCGCCGGAGCGTTACCTGATGGAACGTTTTATCACTTCGCCTGTTTGGGTAGATGGCGAGATGCGCAACGGCG
TTATCCGCAATGCGCGTCTTAAACCCCACCCCGACTATCGACCGCCGCTAAAATGGGTCTCGCTGGATATCGAAACCACG
CGACACGGCGAGCTGTATTGCATTGGTCTGGAAGGCTGCGGCCAGCGTACCGTTTATATGCTGGGCCCGGCGAACGGCGA
TGAGCGCCAACTGGACTTCGAGCTTGTGTATGTCGCCAGCCGCCCGCAGTTGCTGGAAAAACTCAACGCCTGGTTTGCCG
AACATGATCCTGACGTCATCATCGGCTGGAACGTGGTGCAGTTCGACCTGCGTATGCTGCAAAAATATGCCGAACGCTAC
CGTATCCCTTTACGGCTGGGGCGAGACAATAGCGAACTGGAGTGGCGCGAACATGGCTTCAAAAACGGCGTTTTTTTCGC
CCAGGCCAGAGGGCGAGTCATTATCGATGGTATCGACGCGCTTAAATCCGCGTTCTGGAATTTTTCGTCTTTTTCGCTGG
AAGCCGTCTCGCAGGAACTGCTCGGCGAAGGAAAATCCATTGATAACCCCTGGGATCGTATGGATGAGATCGATCGCCGC
TTTGCCCAGGATAAACCCGCCCTCGCCACCTACAACCTGAAAGACTGCGAGCTGGTCACGCGCATTTTCCATAAAACCGA
AATCATGCCGTTTTTGTTGGAACGCGCGACGATCAACGGCCTGCCGGTCGATCGCCACGGCGGTTCGGTCGCCGCATTCG
GGCATCTTTACTTTCCGCGAATGCACCGCGCCGGCTATATCGCGCCCAATCTCGGCGAAGTGCCGCCGCTGGCAAGTCCC
GGCGGCTACGTAATGGATTCACAACCCGGATTATACGATTCAGTACTGGTGCTGGATTATAAAAGCCTGTACCCGTCAAT
TATCCGCACGTTTTTGATTGATCCCGTCGGGCTGGTCGAAGGCATGGCGCAGCCGGACCCGGAACACAGTACCGAAGGTT
TCCTTGACGCCTGGTTTTCACGAGAAAAGCACTGCCTGCCGGAAATTGTTAGCCAAATCTGGCACGGGCGCGATGAAGCC
AAACGTCAGGGCAATAAACCGCTCTCGCAGGCGCTTAAAATCATTATGAACGCCTTTTATGGCGTACTCGGCACCACGGC
CTGTCGCTTTTTCGATCCCCGTCTGGCGTCGTCAATCACCATGCGCGGTCATGCGATTATGCGCCAGACCAAAGCGCTGA
TTGAGGCGCAGGGTTATGACGTGATTTATGGCGATACCGATTCCACTTTCGTCTGGCTCCGGCGCGCGCATTCAGAAGCC
GATGCGGCGGAAATCGGGCACAGGCTGGTGCGTCACGTGAACGAATGGTGGGCGCAGACGTTACAACAGCAAAATTTGAC
CAGCGCGCTGGAACTGGAGTTTGAAACTCACTTTTGCCGTTTTCTGATGCCGACCATCCGCGGCGCGGATACTGGCAGTA
AAAAACGCTACGCGGGCCTGATTCAGGAAGGCGACAGCCAGCGCATGGTATTTAAAGGTCTGGAAACGGTGCGGACCGAC
TGGACGCCGCTGGCGCAGCGCTTTCAACAGGAGCTGTATCTGCGCGTTTTTCGCAATGAACCTTATCAAGACTATGTGCG
CGAGACTATCGATAAACTGATGGCGGGCGAACTGGACGCGCAGCTGGTTTATCGTAAGCGTCTGCGTCGCCCTTTACATG
AGTACCAGCGTAACGTTCCGTCGCACGTTCGCGCCGCCCGGCTGGCGGATGAACAAAACCTGAAGCAGGGTCGTCCGGCG
CAGTATCAGAATCGCGGCGCCATAAAATATGTCTGGACTGTTAACGGCCCCGAGCCCGTGGATTACCAGCAGTCGCCGCT
GGATTACGAACACTATCTTACCCGCCAGCTACAACCCGTGGCGGAAGGAATTCTTCCCTTCGTTGAAGATAATTTTGCTA
CACTACTGACCGGGCAACTGGGGTTATTTTGA

Upstream 100 bases:

>100_bases
AGCGGATATCGGCTTTTACGTTATATGCCTGTACAAAATAACAGTATTACCCCCTAAAACAGGTTATAATTAGCGTGAAT
TTTTTAGACAGGTAATCATC

Downstream 100 bases:

>100_bases
CGCGTGACGAAAGCGCCGCCTTCCAGTACCATAGCGCCCTTTCCATTCCTGGACCTAATTAACGCCACTACCACATTAGC
CTGGTAGCGGGGTCGTATAC

Product: DNA polymerase II

Products: NA

Alternate protein names: Pol II [H]

Number of amino acids: Translated: 783; Mature: 782

Protein sequence:

>783_residues
MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQATLAPQESVAFIPTSQTSRAASLLQAEKDYRLTPLQLRDFHRQPVSG
LYCRTHRQLMRMEKLLRENGVTVYEADVRPPERYLMERFITSPVWVDGEMRNGVIRNARLKPHPDYRPPLKWVSLDIETT
RHGELYCIGLEGCGQRTVYMLGPANGDERQLDFELVYVASRPQLLEKLNAWFAEHDPDVIIGWNVVQFDLRMLQKYAERY
RIPLRLGRDNSELEWREHGFKNGVFFAQARGRVIIDGIDALKSAFWNFSSFSLEAVSQELLGEGKSIDNPWDRMDEIDRR
FAQDKPALATYNLKDCELVTRIFHKTEIMPFLLERATINGLPVDRHGGSVAAFGHLYFPRMHRAGYIAPNLGEVPPLASP
GGYVMDSQPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHSTEGFLDAWFSREKHCLPEIVSQIWHGRDEA
KRQGNKPLSQALKIIMNAFYGVLGTTACRFFDPRLASSITMRGHAIMRQTKALIEAQGYDVIYGDTDSTFVWLRRAHSEA
DAAEIGHRLVRHVNEWWAQTLQQQNLTSALELEFETHFCRFLMPTIRGADTGSKKRYAGLIQEGDSQRMVFKGLETVRTD
WTPLAQRFQQELYLRVFRNEPYQDYVRETIDKLMAGELDAQLVYRKRLRRPLHEYQRNVPSHVRAARLADEQNLKQGRPA
QYQNRGAIKYVWTVNGPEPVDYQQSPLDYEHYLTRQLQPVAEGILPFVEDNFATLLTGQLGLF

Sequences:

>Translated_783_residues
MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQATLAPQESVAFIPTSQTSRAASLLQAEKDYRLTPLQLRDFHRQPVSG
LYCRTHRQLMRMEKLLRENGVTVYEADVRPPERYLMERFITSPVWVDGEMRNGVIRNARLKPHPDYRPPLKWVSLDIETT
RHGELYCIGLEGCGQRTVYMLGPANGDERQLDFELVYVASRPQLLEKLNAWFAEHDPDVIIGWNVVQFDLRMLQKYAERY
RIPLRLGRDNSELEWREHGFKNGVFFAQARGRVIIDGIDALKSAFWNFSSFSLEAVSQELLGEGKSIDNPWDRMDEIDRR
FAQDKPALATYNLKDCELVTRIFHKTEIMPFLLERATINGLPVDRHGGSVAAFGHLYFPRMHRAGYIAPNLGEVPPLASP
GGYVMDSQPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHSTEGFLDAWFSREKHCLPEIVSQIWHGRDEA
KRQGNKPLSQALKIIMNAFYGVLGTTACRFFDPRLASSITMRGHAIMRQTKALIEAQGYDVIYGDTDSTFVWLRRAHSEA
DAAEIGHRLVRHVNEWWAQTLQQQNLTSALELEFETHFCRFLMPTIRGADTGSKKRYAGLIQEGDSQRMVFKGLETVRTD
WTPLAQRFQQELYLRVFRNEPYQDYVRETIDKLMAGELDAQLVYRKRLRRPLHEYQRNVPSHVRAARLADEQNLKQGRPA
QYQNRGAIKYVWTVNGPEPVDYQQSPLDYEHYLTRQLQPVAEGILPFVEDNFATLLTGQLGLF
>Mature_782_residues
AQAGFILTRHWRDTPQGTEVSFWLATDNGPLQATLAPQESVAFIPTSQTSRAASLLQAEKDYRLTPLQLRDFHRQPVSGL
YCRTHRQLMRMEKLLRENGVTVYEADVRPPERYLMERFITSPVWVDGEMRNGVIRNARLKPHPDYRPPLKWVSLDIETTR
HGELYCIGLEGCGQRTVYMLGPANGDERQLDFELVYVASRPQLLEKLNAWFAEHDPDVIIGWNVVQFDLRMLQKYAERYR
IPLRLGRDNSELEWREHGFKNGVFFAQARGRVIIDGIDALKSAFWNFSSFSLEAVSQELLGEGKSIDNPWDRMDEIDRRF
AQDKPALATYNLKDCELVTRIFHKTEIMPFLLERATINGLPVDRHGGSVAAFGHLYFPRMHRAGYIAPNLGEVPPLASPG
GYVMDSQPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHSTEGFLDAWFSREKHCLPEIVSQIWHGRDEAK
RQGNKPLSQALKIIMNAFYGVLGTTACRFFDPRLASSITMRGHAIMRQTKALIEAQGYDVIYGDTDSTFVWLRRAHSEAD
AAEIGHRLVRHVNEWWAQTLQQQNLTSALELEFETHFCRFLMPTIRGADTGSKKRYAGLIQEGDSQRMVFKGLETVRTDW
TPLAQRFQQELYLRVFRNEPYQDYVRETIDKLMAGELDAQLVYRKRLRRPLHEYQRNVPSHVRAARLADEQNLKQGRPAQ
YQNRGAIKYVWTVNGPEPVDYQQSPLDYEHYLTRQLQPVAEGILPFVEDNFATLLTGQLGLF

Specific function: Thought to be involved in DNA repair and/or mutagenesis [H]

COG id: COG0417

COG function: function code L; DNA polymerase elongation subunit (family B)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA polymerase type-B family [H]

Homologues:

Organism=Homo sapiens, GI156616275, Length=657, Percent_Identity=26.7884322678843, Blast_Score=135, Evalue=2e-31,
Organism=Homo sapiens, GI106507301, Length=643, Percent_Identity=24.5723172628305, Blast_Score=122, Evalue=2e-27,
Organism=Escherichia coli, GI1786246, Length=783, Percent_Identity=89.7828863346105, Blast_Score=1482, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17559488, Length=714, Percent_Identity=25.9103641456583, Blast_Score=145, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI32565317, Length=784, Percent_Identity=23.3418367346939, Blast_Score=133, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6320101, Length=632, Percent_Identity=25.6329113924051, Blast_Score=126, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6324227, Length=549, Percent_Identity=23.3151183970856, Blast_Score=93, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6325090, Length=657, Percent_Identity=21.9178082191781, Blast_Score=81, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24664937, Length=808, Percent_Identity=23.5148514851485, Blast_Score=122, Evalue=9e-28,
Organism=Drosophila melanogaster, GI24648780, Length=640, Percent_Identity=23.90625, Blast_Score=120, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006172
- InterPro:   IPR017964
- InterPro:   IPR006133
- InterPro:   IPR006134
- InterPro:   IPR023211
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00136 DNA_pol_B; PF03104 DNA_pol_B_exo [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 90081; Mature: 89949

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: PS00116 DNA_POLYMERASE_B

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQATLAPQESVAFIPTSQTSRAASLLQAE
CCCCCEEEEECCCCCCCCCEEEEEEEECCCCEEEEECCCCCEEEEECCCHHHHHHHHHHC
KDYRLTPLQLRDFHRQPVSGLYCRTHRQLMRMEKLLRENGVTVYEADVRPPERYLMERFI
CCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHH
TSPVWVDGEMRNGVIRNARLKPHPDYRPPLKWVSLDIETTRHGELYCIGLEGCGQRTVYM
CCCEEECCHHHCCCEECCCCCCCCCCCCCCEEEEEEEEECCCCCEEEEEECCCCCEEEEE
LGPANGDERQLDFELVYVASRPQLLEKLNAWFAEHDPDVIIGWNVVQFDLRMLQKYAERY
EECCCCCCCEEEEEEEEEECCHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHC
RIPLRLGRDNSELEWREHGFKNGVFFAQARGRVIIDGIDALKSAFWNFSSFSLEAVSQEL
CCCEEECCCCCCCHHHHCCCCCCEEEEECCCCEEEECHHHHHHHHCCCCCHHHHHHHHHH
LGEGKSIDNPWDRMDEIDRRFAQDKPALATYNLKDCELVTRIFHKTEIMPFLLERATING
HCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
LPVDRHGGSVAAFGHLYFPRMHRAGYIAPNLGEVPPLASPGGYVMDSQPGLYDSVLVLDY
CCCCCCCCCEEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCCCCHHHHEEEH
KSLYPSIIRTFLIDPVGLVEGMAQPDPEHSTEGFLDAWFSREKHCLPEIVSQIWHGRDEA
HHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCHHH
KRQGNKPLSQALKIIMNAFYGVLGTTACRFFDPRLASSITMRGHAIMRQTKALIEAQGYD
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCE
VIYGDTDSTFVWLRRAHSEADAAEIGHRLVRHVNEWWAQTLQQQNLTSALELEFETHFCR
EEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEHHHHHHHH
FLMPTIRGADTGSKKRYAGLIQEGDSQRMVFKGLETVRTDWTPLAQRFQQELYLRVFRNE
HHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
PYQDYVRETIDKLMAGELDAQLVYRKRLRRPLHEYQRNVPSHVRAARLADEQNLKQGRPA
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCHHHHCCCCCC
QYQNRGAIKYVWTVNGPEPVDYQQSPLDYEHYLTRQLQPVAEGILPFVEDNFATLLTGQL
CCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCHH
GLF
CCC
>Mature Secondary Structure 
AQAGFILTRHWRDTPQGTEVSFWLATDNGPLQATLAPQESVAFIPTSQTSRAASLLQAE
CCCCEEEEECCCCCCCCCEEEEEEEECCCCEEEEECCCCCEEEEECCCHHHHHHHHHHC
KDYRLTPLQLRDFHRQPVSGLYCRTHRQLMRMEKLLRENGVTVYEADVRPPERYLMERFI
CCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHH
TSPVWVDGEMRNGVIRNARLKPHPDYRPPLKWVSLDIETTRHGELYCIGLEGCGQRTVYM
CCCEEECCHHHCCCEECCCCCCCCCCCCCCEEEEEEEEECCCCCEEEEEECCCCCEEEEE
LGPANGDERQLDFELVYVASRPQLLEKLNAWFAEHDPDVIIGWNVVQFDLRMLQKYAERY
EECCCCCCCEEEEEEEEEECCHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHC
RIPLRLGRDNSELEWREHGFKNGVFFAQARGRVIIDGIDALKSAFWNFSSFSLEAVSQEL
CCCEEECCCCCCCHHHHCCCCCCEEEEECCCCEEEECHHHHHHHHCCCCCHHHHHHHHHH
LGEGKSIDNPWDRMDEIDRRFAQDKPALATYNLKDCELVTRIFHKTEIMPFLLERATING
HCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
LPVDRHGGSVAAFGHLYFPRMHRAGYIAPNLGEVPPLASPGGYVMDSQPGLYDSVLVLDY
CCCCCCCCCEEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCCCCHHHHEEEH
KSLYPSIIRTFLIDPVGLVEGMAQPDPEHSTEGFLDAWFSREKHCLPEIVSQIWHGRDEA
HHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCHHH
KRQGNKPLSQALKIIMNAFYGVLGTTACRFFDPRLASSITMRGHAIMRQTKALIEAQGYD
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCE
VIYGDTDSTFVWLRRAHSEADAAEIGHRLVRHVNEWWAQTLQQQNLTSALELEFETHFCR
EEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEHHHHHHHH
FLMPTIRGADTGSKKRYAGLIQEGDSQRMVFKGLETVRTDWTPLAQRFQQELYLRVFRNE
HHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
PYQDYVRETIDKLMAGELDAQLVYRKRLRRPLHEYQRNVPSHVRAARLADEQNLKQGRPA
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCHHHHCCCCCC
QYQNRGAIKYVWTVNGPEPVDYQQSPLDYEHYLTRQLQPVAEGILPFVEDNFATLLTGQL
CCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCHH
GLF
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2034216; 2261080; 1630901; 9278503; 2217198; 9079692 [H]