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The map label for this gene is leuO [H]

Identifier: 29140574

GI number: 29140574

Start: 33368

End: 34363

Strand: Direct

Name: leuO [H]

Synonym: t0032

Alternate gene names: 29140574

Gene position: 33368-34363 (Clockwise)

Preceding gene: 29140572

Following gene: 29140576

Centisome position: 0.7

GC content: 38.45

Gene sequence:

>996_bases
ATGCGGCCAATAAAAAATGCTAAAAAAATTGACTACAATCTGATCAAAGTGTTCGATACGGTTATTACTGAAGGAAATGC
AACCAGGGCTGCGAGGAAACTGGATGTCACGCCTGCGGCGATCTCTCAGGCTCTTCTTCGTTTACAAAATCTTTATGGCG
AAGAGTTATTTATCAGAACCCGCAAAGGATTAGTCCCGTCCAGCAAAGGTAAATCGCTTCACCAGGTATTTCGCCAGGCA
ATTGAATCTATAGAAAGCACACTGTGCGATAAAACAGATGCGCAGGAGAGTAATGAACTCATTGTTCTGGGAAGTGATAT
CACTGAAAATTACTATTTTCCGGCATTGCTGGATACTGTGTTGATGAATCGATATATTATTAAACACTATGCGATTAAAA
AAACAGGGGAATACTCGCCAGCCTCCATGCTGACGCATGGCTATGCGGATATCATCATGGGAATTCTGGAAATTAAGAAT
GAGATGATCGAAAGTTTTCTTATTGATAATTTATCTGATTTTGTTTGTGTTTGTGGTGAAAAAAGTCCATTGGTTGGGCT
TGAAAAAATGTCTTTATATAATTTTTATGCTGCCAGACATGCTGTCTATCATTCAGATATGTTCTCTTCTTTCAATGCTG
ATAGCATTGATTTATTCAAGAGCAGTACGCCTTATGCGGGGCGCAGGGAAATAGGTTATTATAGTGATTCACTATTTGGC
GTTATCGGTGTTGTTGAAAAAAGCGATATGGTTGCGATTTTGCCAGGAAAAATTGCTACTTATTTTAGAGATGTGCGGCG
TTATAATATAAAAATACTACGTATGCCTGATGAAATAATTTTTCGTACATTACCCGTTTATGCTTATCTGGCTACAAATA
GCACCCATTATAAAAATGCCAAAAAACTGATATCAACATTTCAGTCGACCTTTCTTTTTAGCCAGGAAAAACAGCCTGAC
GCTTTGGTTGAAGGAAGCACATCCTTATGCGATTGA

Upstream 100 bases:

>100_bases
ACAGATAGAGGAAATGATTTACTTATTGAAATAAGTGTGGTGCAAGGTTAATCTGCGCCTTAAATGATGGTGCCAGATTA
TACCAGCCAGGATAACCTAT

Downstream 100 bases:

>100_bases
TCTGTTTAATATCAAATTACTGTATTAAATAAATAACCATTATGACCATGGTCAGTAAAGCAAAAAATAATGCTATATAT
TTATCCCCTTCTTTTTTTAA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MRPIKNAKKIDYNLIKVFDTVITEGNATRAARKLDVTPAAISQALLRLQNLYGEELFIRTRKGLVPSSKGKSLHQVFRQA
IESIESTLCDKTDAQESNELIVLGSDITENYYFPALLDTVLMNRYIIKHYAIKKTGEYSPASMLTHGYADIIMGILEIKN
EMIESFLIDNLSDFVCVCGEKSPLVGLEKMSLYNFYAARHAVYHSDMFSSFNADSIDLFKSSTPYAGRREIGYYSDSLFG
VIGVVEKSDMVAILPGKIATYFRDVRRYNIKILRMPDEIIFRTLPVYAYLATNSTHYKNAKKLISTFQSTFLFSQEKQPD
ALVEGSTSLCD

Sequences:

>Translated_331_residues
MRPIKNAKKIDYNLIKVFDTVITEGNATRAARKLDVTPAAISQALLRLQNLYGEELFIRTRKGLVPSSKGKSLHQVFRQA
IESIESTLCDKTDAQESNELIVLGSDITENYYFPALLDTVLMNRYIIKHYAIKKTGEYSPASMLTHGYADIIMGILEIKN
EMIESFLIDNLSDFVCVCGEKSPLVGLEKMSLYNFYAARHAVYHSDMFSSFNADSIDLFKSSTPYAGRREIGYYSDSLFG
VIGVVEKSDMVAILPGKIATYFRDVRRYNIKILRMPDEIIFRTLPVYAYLATNSTHYKNAKKLISTFQSTFLFSQEKQPD
ALVEGSTSLCD
>Mature_331_residues
MRPIKNAKKIDYNLIKVFDTVITEGNATRAARKLDVTPAAISQALLRLQNLYGEELFIRTRKGLVPSSKGKSLHQVFRQA
IESIESTLCDKTDAQESNELIVLGSDITENYYFPALLDTVLMNRYIIKHYAIKKTGEYSPASMLTHGYADIIMGILEIKN
EMIESFLIDNLSDFVCVCGEKSPLVGLEKMSLYNFYAARHAVYHSDMFSSFNADSIDLFKSSTPYAGRREIGYYSDSLFG
VIGVVEKSDMVAILPGKIATYFRDVRRYNIKILRMPDEIIFRTLPVYAYLATNSTHYKNAKKLISTFQSTFLFSQEKQPD
ALVEGSTSLCD

Specific function: Unknown

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081766, Length=266, Percent_Identity=25.5639097744361, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87081684, Length=281, Percent_Identity=24.1992882562278, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1786820, Length=265, Percent_Identity=21.5094339622642, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 37420; Mature: 37420

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPIKNAKKIDYNLIKVFDTVITEGNATRAARKLDVTPAAISQALLRLQNLYGEELFIRT
CCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEH
RKGLVPSSKGKSLHQVFRQAIESIESTLCDKTDAQESNELIVLGSDITENYYFPALLDTV
HCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHH
LMNRYIIKHYAIKKTGEYSPASMLTHGYADIIMGILEIKNEMIESFLIDNLSDFVCVCGE
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEECCC
KSPLVGLEKMSLYNFYAARHAVYHSDMFSSFNADSIDLFKSSTPYAGRREIGYYSDSLFG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCHHHHHH
VIGVVEKSDMVAILPGKIATYFRDVRRYNIKILRMPDEIIFRTLPVYAYLATNSTHYKNA
HHHEECCCCEEEECCHHHHHHHHHHHHHCEEEEECCHHHHHHHHHHEEEEECCCHHHHHH
KKLISTFQSTFLFSQEKQPDALVEGSTSLCD
HHHHHHHHHHHHHCCCCCCCHHHCCCCCCCC
>Mature Secondary Structure
MRPIKNAKKIDYNLIKVFDTVITEGNATRAARKLDVTPAAISQALLRLQNLYGEELFIRT
CCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEH
RKGLVPSSKGKSLHQVFRQAIESIESTLCDKTDAQESNELIVLGSDITENYYFPALLDTV
HCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHH
LMNRYIIKHYAIKKTGEYSPASMLTHGYADIIMGILEIKNEMIESFLIDNLSDFVCVCGE
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEECCC
KSPLVGLEKMSLYNFYAARHAVYHSDMFSSFNADSIDLFKSSTPYAGRREIGYYSDSLFG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCHHHHHH
VIGVVEKSDMVAILPGKIATYFRDVRRYNIKILRMPDEIIFRTLPVYAYLATNSTHYKNA
HHHEECCCCEEEECCHHHHHHHHHHHHHCEEEEECCHHHHHHHHHHEEEEECCCHHHHHH
KKLISTFQSTFLFSQEKQPDALVEGSTSLCD
HHHHHHHHHHHHHCCCCCCCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609; 3519576 [H]