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The map label for this gene is yaaJ [H]

Identifier: 29140549

GI number: 29140549

Start: 5966

End: 7396

Strand: Reverse

Name: yaaJ [H]

Synonym: t0006

Alternate gene names: 29140549

Gene position: 7396-5966 (Counterclockwise)

Preceding gene: 29140552

Following gene: 29140548

Centisome position: 0.15

GC content: 55.7

Gene sequence:

>1431_bases
ATGCCTGAGTTTTTCAGTTTTATTAACGAAATACTCTGGGGCTCGGTAATGATTTACCTGCTGCTCGGCGCAGGATGTTG
GTTTACCTGGCGTACCGGATTCATTCAGTTTCGTTATATTCGCCAATTTAGCCGCAGTCTGAAAGGCAGCCTTAGCCCGC
AGCCAGGCGGCCTGACGTCATTTCAGGCGCTGTGTACCAGCCTCGCGGCGCGGATTGGCAGCGGTAATCTGGCCGGCGTG
GCGCTGGCTATCGCCGCAGGCGGTCCCGGCGCAGTATTCTGGATGTGGGTCTCGGCCATCATTGGTATGGCGACCTCCTT
CGCCGAGTGTTCGCTTGCCCAGCTTTATAAAGAACGTGACCCGACAGGCCAGTTCCGCGGCGGCCCGGCGTGGTATATGG
CGCGCGGGCTGGGAATGCGCTGGATGGGCGTGGTTTTCGCCCTCTTTCTGCTCGTCGCCTACGGGTTGATTTTTAATAGC
GTGCAGGCGAATGCCGTCTCACGCGCGCTCCATTTCGCCTTCAACATTCCGCCGCTTATCTCCGGCATTGCTTTGGCGTT
CTGTGCCCTGTTAATTATCATCCGCGGCATAAAAGGCGTCGCCCGCCTGATGCAATGGCTGATTCCCCTCATCGCGTTAT
TGTGGGTCGCTGGCAGCGTATTCATTTGCCTCTGGCATATTGAGCAAATGCCTGGCGTTATCGCCTCGATAGTCAAAAGC
GCATTTGGCTGGCAGGAAGCCGCGGCAGGCGCAGCCGGATATACGCTCACCCAGGCTATTACCAGCGGTTTTCAGCGTGG
GATGTTCTCTAATGAAGCGGGAATGGGGTCAACGCCTAATGCGGCCGCAGCGGCAACGTCGTATCCCCCTCACCCTGTCG
CGCAGGGGATTGTGCAAATGATTGGCGTATTCAGCGACACCATTATCATCTGTACCGCCAGCGCGATGATTATCCTGCTG
GCGGGGAATCACGCGTCCCATTCGTCAACAGAAGGCATTCAGCTTCTCCAACATGCAATGGTTTCGTTGACCGGTGAATG
GGGCGCCAGTTTTGTCGCGCTTATCGTCATTCTGTTCGCGTTCAGTTCTATCGTCGCCAACTATATTTACGCCGAAAACA
ATCTCTTTTTTCTGCGGTTACATAACGCAAAGGCCATCTGGCTATTACGTCTTGCGACGCTTGGCATGGTCATCGCCGGT
ACGCTTATCAGTTTTCCGCTGATATGGCAGCTCGCTGACATGATCATGGCCTGTATGGCCATTACCAATCTGACGGCGAT
TTTGTTGCTCTCACCGGTGGTGTATACCCTCGCCGGCGATTATCTGCGACAACGGAAACTGGGCGTAAGACCGCAGTTCG
ATCCGCGGCGCTTCCCGGATATCGAACCACAACTGGCGCCAGATACCTGGGATGCGGCATCGCGCGATTAA

Upstream 100 bases:

>100_bases
GTAAACTACCGTTAATATTAGCCTGACAAGGTCATCTACATCATGGGTCACGCTTATACAGGATCATCAAAGCGTGTCGG
CATCAACAAGAGGGACGTTA

Downstream 100 bases:

>100_bases
GCCGACAATCGCAACCATCGGTCAACTCTGCGGCGTTTTTTTGCTAAAGTCGCAGAAAATTCCCTGCAAGGACTGGATAT
GCTGATTCTGATTTCACCTG

Product: amino-acid transport protein

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MPEFFSFINEILWGSVMIYLLLGAGCWFTWRTGFIQFRYIRQFSRSLKGSLSPQPGGLTSFQALCTSLAARIGSGNLAGV
ALAIAAGGPGAVFWMWVSAIIGMATSFAECSLAQLYKERDPTGQFRGGPAWYMARGLGMRWMGVVFALFLLVAYGLIFNS
VQANAVSRALHFAFNIPPLISGIALAFCALLIIIRGIKGVARLMQWLIPLIALLWVAGSVFICLWHIEQMPGVIASIVKS
AFGWQEAAAGAAGYTLTQAITSGFQRGMFSNEAGMGSTPNAAAAATSYPPHPVAQGIVQMIGVFSDTIIICTASAMIILL
AGNHASHSSTEGIQLLQHAMVSLTGEWGASFVALIVILFAFSSIVANYIYAENNLFFLRLHNAKAIWLLRLATLGMVIAG
TLISFPLIWQLADMIMACMAITNLTAILLLSPVVYTLAGDYLRQRKLGVRPQFDPRRFPDIEPQLAPDTWDAASRD

Sequences:

>Translated_476_residues
MPEFFSFINEILWGSVMIYLLLGAGCWFTWRTGFIQFRYIRQFSRSLKGSLSPQPGGLTSFQALCTSLAARIGSGNLAGV
ALAIAAGGPGAVFWMWVSAIIGMATSFAECSLAQLYKERDPTGQFRGGPAWYMARGLGMRWMGVVFALFLLVAYGLIFNS
VQANAVSRALHFAFNIPPLISGIALAFCALLIIIRGIKGVARLMQWLIPLIALLWVAGSVFICLWHIEQMPGVIASIVKS
AFGWQEAAAGAAGYTLTQAITSGFQRGMFSNEAGMGSTPNAAAAATSYPPHPVAQGIVQMIGVFSDTIIICTASAMIILL
AGNHASHSSTEGIQLLQHAMVSLTGEWGASFVALIVILFAFSSIVANYIYAENNLFFLRLHNAKAIWLLRLATLGMVIAG
TLISFPLIWQLADMIMACMAITNLTAILLLSPVVYTLAGDYLRQRKLGVRPQFDPRRFPDIEPQLAPDTWDAASRD
>Mature_475_residues
PEFFSFINEILWGSVMIYLLLGAGCWFTWRTGFIQFRYIRQFSRSLKGSLSPQPGGLTSFQALCTSLAARIGSGNLAGVA
LAIAAGGPGAVFWMWVSAIIGMATSFAECSLAQLYKERDPTGQFRGGPAWYMARGLGMRWMGVVFALFLLVAYGLIFNSV
QANAVSRALHFAFNIPPLISGIALAFCALLIIIRGIKGVARLMQWLIPLIALLWVAGSVFICLWHIEQMPGVIASIVKSA
FGWQEAAAGAAGYTLTQAITSGFQRGMFSNEAGMGSTPNAAAAATSYPPHPVAQGIVQMIGVFSDTIIICTASAMIILLA
GNHASHSSTEGIQLLQHAMVSLTGEWGASFVALIVILFAFSSIVANYIYAENNLFFLRLHNAKAIWLLRLATLGMVIAGT
LISFPLIWQLADMIMACMAITNLTAILLLSPVVYTLAGDYLRQRKLGVRPQFDPRRFPDIEPQLAPDTWDAASRD

Specific function: Unknown

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=476, Percent_Identity=76.2605042016807, Blast_Score=715, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 51494; Mature: 51363

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: PS00873 NA_ALANINE_SYMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PEFFSFINEILWGSVMIYLLLGAGCWFTWRTGFIQFRYIRQFSRSLKGSLSPQPGGLTS
CHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
FQALCTSLAARIGSGNLAGVALAIAAGGPGAVFWMWVSAIIGMATSFAECSLAQLYKERD
HHHHHHHHHHHHCCCCHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PTGQFRGGPAWYMARGLGMRWMGVVFALFLLVAYGLIFNSVQANAVSRALHFAFNIPPLI
CCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
SGIALAFCALLIIIRGIKGVARLMQWLIPLIALLWVAGSVFICLWHIEQMPGVIASIVKS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFGWQEAAAGAAGYTLTQAITSGFQRGMFSNEAGMGSTPNAAAAATSYPPHPVAQGIVQM
HHCHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHH
IGVFSDTIIICTASAMIILLAGNHASHSSTEGIQLLQHAMVSLTGEWGASFVALIVILFA
HHHHHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
FSSIVANYIYAENNLFFLRLHNAKAIWLLRLATLGMVIAGTLISFPLIWQLADMIMACMA
HHHHHHHHHEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ITNLTAILLLSPVVYTLAGDYLRQRKLGVRPQFDPRRFPDIEPQLAPDTWDAASRD
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1630901; 9278503 [H]