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The map label for this gene is aspS [H]

Identifier: 28572907

GI number: 28572907

Start: 862885

End: 864183

Strand: Reverse

Name: aspS [H]

Synonym: TW766

Alternate gene names: 28572907

Gene position: 864183-862885 (Counterclockwise)

Preceding gene: 28572913

Following gene: 28572903

Centisome position: 93.33

GC content: 46.11

Gene sequence:

>1299_bases
ATGCCAAGTGTTTTGATTGCTCATTTATCGCAGTATCAGTCCTCACAGGCGGTCCAGGTGCGCGGGTGGGTTGAAGCCGT
GCGTGACCAGAAAAATATTCAGTTCGTCATAATACGCGACGAGTCTGGGGCGCCTCTCCAGCTGTTATACGTGCGGAGCG
GTAAGAGCGACTGTATTGCAGAGAAAATTTCATCGCTATCTATCGGATCTTTTGTAAGGGCTTCTGGTGTTCTGAAGCAT
AATGAACGCGTTAAGCTCCGCGGGCTGGAAGTTGAAATCAACAATCTCGATGTTGTTAGTACGGCCTTGCCGAGCCCTAT
TGCCCACGATTCGTCGATTGATAAAAGACTTGACTGGCGCTTTCTCGACCTGAGGAATGAGCGCAATAATTTAATTTTTC
GCATCCAGACATGCTTTTTACATGCGCTTCGTACTTGGTGGGTTAGAAATGGTTTTATTGAAATCAATACACCCAAACTA
ATGGCATCATCAAGCGAGTCTCGAGCAGAGTTATTCAGTCTCAAATATTTTGACAGGATCGGTTATCTTGCCCAGAGTCC
TCAGTTTTTCAAACAAATGTCCCAAGCTGCCGGGTTTGGCAAGGTGTTTGAAATTGCACCTGTTTATCGTGCTGACCCAT
CTTTTACGAGTCGCCATGCAACTGAATATACGAGCATCGACTCCGAGCTCAGTTGGATAGAAAGCCACGAGGATGTAATG
AATATGCAAGAAGAGCTTTTGACATATTCTATATGCGACGTTTTGGAGAGGCACTCAGAGGAAATTAGGGGAGTGTTTGG
TTTGAATCTCTGCCCACCCAAACGTCCCTTCGTCCGGGTTACGCTTGAGCAAGCACTCAACATCTCTGCCAGGTCAGGTC
ATATTTCACAAAGACATGATCTTGATCCAGAGGCAGAGCGACGAGTCGGAAAATGGGCATTAGATAATCACGCGTCAGAT
TTTGTATTTGTTACTAACTATCCGGTTTCTGTAAGGCCTTTCTACCATATGCGAGAAAATGAAAATATAACAAAAAGTTA
TGACCTTCTGTTTAGGGGAATTGAAATAGCCACCGGTGCGCAGCGTGAGCACAGATATGATGTCCTAGTAGACCAAGCGC
GAGAAAAAGGGTTGGATCTCTCAGAGCTTGGGTATTATCTCGATTTTTTTCGCTATGGCATGCCACCCCACGGTGGGTTC
GGTGCTGGGCTTGCAAGGATAATCATGGCACTCTTGCGTTTGGGAAGCATTAAAGAGAGCACTTTTCTGTTTAGGGGTCC
CAACAGGCTTTTGCCGTGA

Upstream 100 bases:

>100_bases
GCGTGGCGGACTAAGTGAATCATGGTCACTTATACAAGTCTAGGGTAGTATTTCGTAGGTGTGTGACAGAGAGATAGTAA
ATTCGAGTTGGTCTTCAGAG

Downstream 100 bases:

>100_bases
GGCATAGCGCGGGCTTAAGATCACTTACGCTTTCGCTTGAATTTCCTCTTACTAGGTCCTGTTGACGAAGGACCCCTCGT
TGCCTTTATAAGTTCAATAC

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MPSVLIAHLSQYQSSQAVQVRGWVEAVRDQKNIQFVIIRDESGAPLQLLYVRSGKSDCIAEKISSLSIGSFVRASGVLKH
NERVKLRGLEVEINNLDVVSTALPSPIAHDSSIDKRLDWRFLDLRNERNNLIFRIQTCFLHALRTWWVRNGFIEINTPKL
MASSSESRAELFSLKYFDRIGYLAQSPQFFKQMSQAAGFGKVFEIAPVYRADPSFTSRHATEYTSIDSELSWIESHEDVM
NMQEELLTYSICDVLERHSEEIRGVFGLNLCPPKRPFVRVTLEQALNISARSGHISQRHDLDPEAERRVGKWALDNHASD
FVFVTNYPVSVRPFYHMRENENITKSYDLLFRGIEIATGAQREHRYDVLVDQAREKGLDLSELGYYLDFFRYGMPPHGGF
GAGLARIIMALLRLGSIKESTFLFRGPNRLLP

Sequences:

>Translated_432_residues
MPSVLIAHLSQYQSSQAVQVRGWVEAVRDQKNIQFVIIRDESGAPLQLLYVRSGKSDCIAEKISSLSIGSFVRASGVLKH
NERVKLRGLEVEINNLDVVSTALPSPIAHDSSIDKRLDWRFLDLRNERNNLIFRIQTCFLHALRTWWVRNGFIEINTPKL
MASSSESRAELFSLKYFDRIGYLAQSPQFFKQMSQAAGFGKVFEIAPVYRADPSFTSRHATEYTSIDSELSWIESHEDVM
NMQEELLTYSICDVLERHSEEIRGVFGLNLCPPKRPFVRVTLEQALNISARSGHISQRHDLDPEAERRVGKWALDNHASD
FVFVTNYPVSVRPFYHMRENENITKSYDLLFRGIEIATGAQREHRYDVLVDQAREKGLDLSELGYYLDFFRYGMPPHGGF
GAGLARIIMALLRLGSIKESTFLFRGPNRLLP
>Mature_431_residues
PSVLIAHLSQYQSSQAVQVRGWVEAVRDQKNIQFVIIRDESGAPLQLLYVRSGKSDCIAEKISSLSIGSFVRASGVLKHN
ERVKLRGLEVEINNLDVVSTALPSPIAHDSSIDKRLDWRFLDLRNERNNLIFRIQTCFLHALRTWWVRNGFIEINTPKLM
ASSSESRAELFSLKYFDRIGYLAQSPQFFKQMSQAAGFGKVFEIAPVYRADPSFTSRHATEYTSIDSELSWIESHEDVMN
MQEELLTYSICDVLERHSEEIRGVFGLNLCPPKRPFVRVTLEQALNISARSGHISQRHDLDPEAERRVGKWALDNHASDF
VFVTNYPVSVRPFYHMRENENITKSYDLLFRGIEIATGAQREHRYDVLVDQAREKGLDLSELGYYLDFFRYGMPPHGGFG
AGLARIIMALLRLGSIKESTFLFRGPNRLLP

Specific function: Unknown

COG id: COG0017

COG function: function code J; Aspartyl/asparaginyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI45439306, Length=456, Percent_Identity=32.0175438596491, Blast_Score=241, Evalue=9e-64,
Organism=Homo sapiens, GI4758762, Length=452, Percent_Identity=28.3185840707965, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI226491502, Length=459, Percent_Identity=28.7581699346405, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI40789249, Length=247, Percent_Identity=24.6963562753036, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI1787161, Length=453, Percent_Identity=26.2693156732892, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI1790571, Length=434, Percent_Identity=22.5806451612903, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789256, Length=437, Percent_Identity=23.1121281464531, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788173, Length=232, Percent_Identity=25.8620689655172, Blast_Score=75, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17551876, Length=455, Percent_Identity=31.6483516483516, Blast_Score=229, Evalue=3e-60,
Organism=Caenorhabditis elegans, GI71984122, Length=439, Percent_Identity=26.879271070615, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17556372, Length=417, Percent_Identity=26.6187050359712, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI32566633, Length=296, Percent_Identity=25, Blast_Score=90, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323011, Length=458, Percent_Identity=33.4061135371179, Blast_Score=228, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6321807, Length=438, Percent_Identity=28.0821917808219, Blast_Score=155, Evalue=9e-39,
Organism=Saccharomyces cerevisiae, GI6319872, Length=478, Percent_Identity=24.2677824267782, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6325153, Length=307, Percent_Identity=25.0814332247557, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17136276, Length=461, Percent_Identity=30.3687635574837, Blast_Score=218, Evalue=6e-57,
Organism=Drosophila melanogaster, GI19921528, Length=435, Percent_Identity=28.0459770114943, Blast_Score=152, Evalue=5e-37,
Organism=Drosophila melanogaster, GI19922416, Length=451, Percent_Identity=22.1729490022173, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24584738, Length=293, Percent_Identity=27.9863481228669, Blast_Score=82, Evalue=6e-16,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020780
- InterPro:   IPR004523
- InterPro:   IPR002312
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 49421; Mature: 49290

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSVLIAHLSQYQSSQAVQVRGWVEAVRDQKNIQFVIIRDESGAPLQLLYVRSGKSDCIA
CCCHHHHHHHHHCCCCEEEHHHHHHHHHCCCCEEEEEEECCCCCCEEEEEEECCCCHHHH
EKISSLSIGSFVRASGVLKHNERVKLRGLEVEINNLDVVSTALPSPIAHDSSIDKRLDWR
HHHHHCCHHHHHHHCCCEECCCEEEEEEEEEEECCCEEEHHHCCCCCCCCCCCHHHCCEE
FLDLRNERNNLIFRIQTCFLHALRTWWVRNGFIEINTPKLMASSSESRAELFSLKYFDRI
EEEECCCCCCEEEEEHHHHHHHHHHHHHHCCEEEECCCCEEECCCCHHHHHHHHHHHHHH
GYLAQSPQFFKQMSQAAGFGKVFEIAPVYRADPSFTSRHATEYTSIDSELSWIESHEDVM
HHHHCCCHHHHHHHHHCCCCCEEEECCCEECCCCCCCCCCHHHHCCCHHHHHHHHHHHHH
NMQEELLTYSICDVLERHSEEIRGVFGLNLCPPKRPFVRVTLEQALNISARSGHISQRHD
HHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCEEEEEEHHHHCCCCCCCCCCCCCC
LDPEAERRVGKWALDNHASDFVFVTNYPVSVRPFYHMRENENITKSYDLLFRGIEIATGA
CCCHHHHHHCCHHHCCCCCCEEEEECCCEEECCEEEECCCCCCHHHHHHHHHCHHEECCC
QREHRYDVLVDQAREKGLDLSELGYYLDFFRYGMPPHGGFGAGLARIIMALLRLGSIKES
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCC
TFLFRGPNRLLP
EEEEECCCCCCC
>Mature Secondary Structure 
PSVLIAHLSQYQSSQAVQVRGWVEAVRDQKNIQFVIIRDESGAPLQLLYVRSGKSDCIA
CCHHHHHHHHHCCCCEEEHHHHHHHHHCCCCEEEEEEECCCCCCEEEEEEECCCCHHHH
EKISSLSIGSFVRASGVLKHNERVKLRGLEVEINNLDVVSTALPSPIAHDSSIDKRLDWR
HHHHHCCHHHHHHHCCCEECCCEEEEEEEEEEECCCEEEHHHCCCCCCCCCCCHHHCCEE
FLDLRNERNNLIFRIQTCFLHALRTWWVRNGFIEINTPKLMASSSESRAELFSLKYFDRI
EEEECCCCCCEEEEEHHHHHHHHHHHHHHCCEEEECCCCEEECCCCHHHHHHHHHHHHHH
GYLAQSPQFFKQMSQAAGFGKVFEIAPVYRADPSFTSRHATEYTSIDSELSWIESHEDVM
HHHHCCCHHHHHHHHHCCCCCEEEECCCEECCCCCCCCCCHHHHCCCHHHHHHHHHHHHH
NMQEELLTYSICDVLERHSEEIRGVFGLNLCPPKRPFVRVTLEQALNISARSGHISQRHD
HHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCEEEEEEHHHHCCCCCCCCCCCCCC
LDPEAERRVGKWALDNHASDFVFVTNYPVSVRPFYHMRENENITKSYDLLFRGIEIATGA
CCCHHHHHHCCHHHCCCCCCEEEEECCCEEECCEEEECCCCCCHHHHHHHHHCHHEECCC
QREHRYDVLVDQAREKGLDLSELGYYLDFFRYGMPPHGGFGAGLARIIMALLRLGSIKES
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCC
TFLFRGPNRLLP
EEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]