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The map label for this gene is murB

Identifier: 28572875

GI number: 28572875

Start: 828402

End: 829457

Strand: Reverse

Name: murB

Synonym: TW734

Alternate gene names: 28572875

Gene position: 829457-828402 (Counterclockwise)

Preceding gene: 28572876

Following gene: 28572873

Centisome position: 89.58

GC content: 47.63

Gene sequence:

>1056_bases
TTGGTTTCTTTTTCTGAGATTACGACCCTCGGCGTCGGTGGCTCAATTGCTTGTTTTATTGAGTGTTCGCCGGATGAGTT
TGTTGAGCGCGCGCCCGGACTGTTTCGCCCCGGTCATCATGTTCTTGTAGTTGGTGGCGGGAGCAATCTTGTTGCTTCTG
ATTGCCCCTTTCCGGGTACAGTTGTTAGATTAAAGAGCCGCGATACTATCATCTCTGATGACGGCAACTATACAAGGTTC
AGCGTTTCGGCTGGTACATCTTGGGATGATCTGGTTAGCTACACGCTTGATTTGGGCTTTGATCAGCTCTCACCCATGTC
TGGCATACCTGGGACTTTTGGCGGCGCGCTTGCCCAGAATATCTCTGCCTACGGTGCAGCTGTGCGTGATGTACTAGGCA
GTGTGGAGGTATATGACGCTTGTACGAGTGAGGTTGTCACTTTCGGCCTTGAAGATATGAGGTACGGATATAGAACATCA
GCTTTAAAAAATGTACGCAATAAGGTTATTCTCGGCGGCACATTATTGTTAAAGCCTGGCCCAACTCCGGTTTTGTATAG
GCAATTGGCTAATGCACTAAAAGTAGACCTTGGAACTTACTGTTCAGGAAAACAGGTAAGAGACCAAGTATTGCGTATCA
GGGCTGAGAAGGGAATGCTCCCCCGATACCTTGTGCCTAAAGGATTTGACGTCTGTAACACATCAAGCGTTGGGTCATTT
TTTGTCAATCCCATTGTCTCAAAAGAACATTTGAGCAGGCTTAGGCGCCTTGTCCCACAGGGTGCGCTGAACAGTTCTGT
TATTCAGACTGATGAGATGGGAGGGGTGAAAGTAAGCGCTGCGTTTCTGCTAGAGCAGAGTGGATTTGAAAAGGGATTTT
GTATTTCCGGATCACAAGCGGCGATTTCAACTCAACATAGCCTTGCGATAGTAAACAGGGGTGGTGCAACTGCCGCCGAA
GTGATTGAACTTGCAGGACTTATTACACGAACCGTCTCAAGAAAATTCGATATACACCTCATTCCTGAGCCGGTTTTTGT
TGGTCTCGAACTGTAG

Upstream 100 bases:

>100_bases
TAGTGACAATATCGATGGCTTCCAGGTTCCGATTGACCCTATGGAGCTTTTGCAGTGCGATAGCTGCCAGTAATCCTGGC
AGCTTGTCTCGTGAATTTCT

Downstream 100 bases:

>100_bases
ACTCAAAATACCTTCGTCATGTCGGAGTTCGCTGTATCAAAGAATTCCACAAGCCGTCCTGTTCCCTCCAGCAAACTTGT
TTCATCTAGGGCGTATGAAA

Product: UDP-N-acetylenolpyruvoylglucosamine reductase

Products: NA

Alternate protein names: UDP-N-acetylmuramate dehydrogenase

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MVSFSEITTLGVGGSIACFIECSPDEFVERAPGLFRPGHHVLVVGGGSNLVASDCPFPGTVVRLKSRDTIISDDGNYTRF
SVSAGTSWDDLVSYTLDLGFDQLSPMSGIPGTFGGALAQNISAYGAAVRDVLGSVEVYDACTSEVVTFGLEDMRYGYRTS
ALKNVRNKVILGGTLLLKPGPTPVLYRQLANALKVDLGTYCSGKQVRDQVLRIRAEKGMLPRYLVPKGFDVCNTSSVGSF
FVNPIVSKEHLSRLRRLVPQGALNSSVIQTDEMGGVKVSAAFLLEQSGFEKGFCISGSQAAISTQHSLAIVNRGGATAAE
VIELAGLITRTVSRKFDIHLIPEPVFVGLEL

Sequences:

>Translated_351_residues
MVSFSEITTLGVGGSIACFIECSPDEFVERAPGLFRPGHHVLVVGGGSNLVASDCPFPGTVVRLKSRDTIISDDGNYTRF
SVSAGTSWDDLVSYTLDLGFDQLSPMSGIPGTFGGALAQNISAYGAAVRDVLGSVEVYDACTSEVVTFGLEDMRYGYRTS
ALKNVRNKVILGGTLLLKPGPTPVLYRQLANALKVDLGTYCSGKQVRDQVLRIRAEKGMLPRYLVPKGFDVCNTSSVGSF
FVNPIVSKEHLSRLRRLVPQGALNSSVIQTDEMGGVKVSAAFLLEQSGFEKGFCISGSQAAISTQHSLAIVNRGGATAAE
VIELAGLITRTVSRKFDIHLIPEPVFVGLEL
>Mature_351_residues
MVSFSEITTLGVGGSIACFIECSPDEFVERAPGLFRPGHHVLVVGGGSNLVASDCPFPGTVVRLKSRDTIISDDGNYTRF
SVSAGTSWDDLVSYTLDLGFDQLSPMSGIPGTFGGALAQNISAYGAAVRDVLGSVEVYDACTSEVVTFGLEDMRYGYRTS
ALKNVRNKVILGGTLLLKPGPTPVLYRQLANALKVDLGTYCSGKQVRDQVLRIRAEKGMLPRYLVPKGFDVCNTSSVGSF
FVNPIVSKEHLSRLRRLVPQGALNSSVIQTDEMGGVKVSAAFLLEQSGFEKGFCISGSQAAISTQHSLAIVNRGGATAAE
VIELAGLITRTVSRKFDIHLIPEPVFVGLEL

Specific function: Cell wall formation

COG id: COG0812

COG function: function code M; UDP-N-acetylmuramate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain

Homologues:

Organism=Escherichia coli, GI1790407, Length=350, Percent_Identity=30, Blast_Score=122, Evalue=5e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURB_TROW8 (Q83HA7)

Other databases:

- EMBL:   BX251412
- RefSeq:   NP_789655.1
- ProteinModelPortal:   Q83HA7
- SMR:   Q83HA7
- STRING:   Q83HA7
- GeneID:   1064934
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW734
- eggNOG:   COG0812
- HOGENOM:   HBG686573
- OMA:   GAAPMQN
- PhylomeDB:   Q83HA7
- ProtClustDB:   CLSK230247
- BioCyc:   TWHI218496:TW0700-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00037
- InterPro:   IPR016169
- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR003170
- InterPro:   IPR011601
- InterPro:   IPR006094
- Gene3D:   G3DSA:3.30.465.10
- Gene3D:   G3DSA:3.30.43.10
- Gene3D:   G3DSA:3.90.78.10
- PANTHER:   PTHR21071
- TIGRFAMs:   TIGR00179

Pfam domain/function: PF01565 FAD_binding_4; PF02873 MurB_C; SSF56176 FAD-binding_2; SSF56194 MurB_C

EC number: =1.1.1.158

Molecular weight: Translated: 37447; Mature: 37447

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: PS51387 FAD_PCMH

Important sites: ACT_SITE 158-158 ACT_SITE 239-239 ACT_SITE 343-343

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSFSEITTLGVGGSIACFIECSPDEFVERAPGLFRPGHHVLVVGGGSNLVASDCPFPGT
CCCHHHEEEECCCCCEEEEEECCHHHHHHHCCCCCCCCCEEEEEECCCCEEECCCCCCCE
VVRLKSRDTIISDDGNYTRFSVSAGTSWDDLVSYTLDLGFDQLSPMSGIPGTFGGALAQN
EEEEECCCEEEECCCCEEEEEEECCCCHHHHHHHHHHCCCHHCCCCCCCCCCHHHHHHHH
ISAYGAAVRDVLGSVEVYDACTSEVVTFGLEDMRYGYRTSALKNVRNKVILGGTLLLKPG
HHHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHHHCCHHHHHHHHHHCEEEECEEEEECCC
PTPVLYRQLANALKVDLGTYCSGKQVRDQVLRIRAEKGMLPRYLVPKGFDVCNTSSVGSF
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCHH
FVNPIVSKEHLSRLRRLVPQGALNSSVIQTDEMGGVKVSAAFLLEQSGFEKGFCISGSQA
HHCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCEEEEHEEEEHHCCCCCCEEECCCCC
AISTQHSLAIVNRGGATAAEVIELAGLITRTVSRKFDIHLIPEPVFVGLEL
EEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEEEEC
>Mature Secondary Structure
MVSFSEITTLGVGGSIACFIECSPDEFVERAPGLFRPGHHVLVVGGGSNLVASDCPFPGT
CCCHHHEEEECCCCCEEEEEECCHHHHHHHCCCCCCCCCEEEEEECCCCEEECCCCCCCE
VVRLKSRDTIISDDGNYTRFSVSAGTSWDDLVSYTLDLGFDQLSPMSGIPGTFGGALAQN
EEEEECCCEEEECCCCEEEEEEECCCCHHHHHHHHHHCCCHHCCCCCCCCCCHHHHHHHH
ISAYGAAVRDVLGSVEVYDACTSEVVTFGLEDMRYGYRTSALKNVRNKVILGGTLLLKPG
HHHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHHHCCHHHHHHHHHHCEEEECEEEEECCC
PTPVLYRQLANALKVDLGTYCSGKQVRDQVLRIRAEKGMLPRYLVPKGFDVCNTSSVGSF
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCHH
FVNPIVSKEHLSRLRRLVPQGALNSSVIQTDEMGGVKVSAAFLLEQSGFEKGFCISGSQA
HHCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCEEEEHEEEEHHCCCCCCEEECCCCC
AISTQHSLAIVNRGGATAAEVIELAGLITRTVSRKFDIHLIPEPVFVGLEL
EEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12606174