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The map label for this gene is dnaX [H]

Identifier: 28572868

GI number: 28572868

Start: 822194

End: 823762

Strand: Reverse

Name: dnaX [H]

Synonym: TW727

Alternate gene names: 28572868

Gene position: 823762-822194 (Counterclockwise)

Preceding gene: 28572869

Following gene: 28572867

Centisome position: 88.97

GC content: 45.76

Gene sequence:

>1569_bases
ATGATGAGGAGTGAGCAGGCTCTTTATAGGCGTTATCGACCCGAGGTGTTTTCTGAGATGGTCGGGCAAGACGGTGTTAC
CGGCCTGTTATCTTCTGCCCTTGATAACAATCGTTTGGGTCATGCCTATCTTTTTAGTGGACCAAGAGGCTGCGGGAAGA
CTACCTCTGCCAGAGTTTTTGCACGATGCCTTAACTGTGTAAATGGTCCAACTTCTAGTCCGTGTGGAAAGTGTCAAAGT
TGTATTGATTTAGGTCGAAGCGGTAACGGGTCACTTGATGTGATTGAGATTGACGCTGCAAGTCATAACGGTGTCGATGA
CGCCCGGGCCTTGCGTGAACGGGCCATTTTTGCTCCGGCGCAGAGTCGGTTTAGGATTTTTATACTTGACGAGGCGCATA
TGGTAACAACTCAAGGGTTCAATGCGCTTTTGAAGATTGTCGAAGAGCCTCCGGCGCATGTAAAGTTCATCTTTGCCACC
ACTGAGCCTGAAAAAGTGCTCGGAACAATACGATCAAGGACATTTTCCTATCCATTTGCTCTTGTTTCCGCAAGTATTAT
GCAGCCATATATTGCGGATATATGCAATAAAGAAAAAATAAGAGTAGAAGATGGGGTACTGCCACTTGTAGTTCGTGCTG
GCGGGGGTTCAGTGAGGGACACATTGTCAATACTTGATCAGCTCATGGTTGGAAAGTGCATTACCTACGAAAGTACGGTT
GGGCTTCTGCGTCTGGCACCAGATATTCTGCTCTACGACCTGTTTTTGGCAATTGCAGAGGATGATGGAGCGACTGCTTT
TGCATCTATTGAAAAAGTTGTGGAAGCCGGGTATGAGCCCGTACAGTTCATCGAAGATCTAATGTCCTGGGTAAAGGATA
TGACTCTTGCTGTTTGTAATGCAGAAGGTGTATTTGACTCCTATGCTCCCGAGAAGGTTGAAAGAATATTTGCACTATCC
AAGCGATTTAGCCTTAAGGATCTTGATGCTATGGCGGAGATTTTGTTTTCTCGGTCGAACAGGCTGTCGGGTCCAATTTC
ACCAAGACTTTTATTAGAGATTATGCTTGCCAGTTTATTACTGGCCGTTGGTAAAAGTAAACTGCAGGTCTCTGATAGAG
TTTCCGACACGCAGCGTCAAAAGGGTTACCCTGGTAATCTCTTGCATAATTGGTCAAGGGTCTTAGACGCTTTAAAGAGC
GAAACTGCAAATATGCTGCAACACTCAAAGCCCTTGCGATTGACTGGAAGTTCATTGACAATCGGTATGCCCCAGGAGAT
TTTATCTCGGTTTGTTGCGAATAAAGCTGCTATCGATGACCTAAAGAGTGTTCTTTACAAGATAACGGGTGTTCAGCTGC
GCCTGTCAGGAAAGCCCATTGATGATCCGGATAACACCGTTAAAACCCCGAGTTCCTGTCTAGATAATGGCACTGTAACT
GATAATAGCGGATACAAAAACACTGAGGTTGACGATGCAGAAACAAAGGAAAAACCTGATAACCCAATTACGGGGGAGGC
TGTTCTGGTGCAGGCTGGTGCAAAACTTATCTCAGAAGAGGAGCAGTAG

Upstream 100 bases:

>100_bases
TACGCTTTCTATGTAACTGGCTGGGAGTCCACCGTTTTTGGGTTCGGTGTAGTTATGATGTCTATGGCAATTTTCTTGCC
CATCCATGTTTTGAAGACAC

Downstream 100 bases:

>100_bases
CTTAGAGAATGTATGTTCGCATAGTTCAGGATCTAATACGTGAGCTTGGCAAGCTACCCGGCATAGGTCCGAAGTCTGCA
CAGCGCATTGCCTTCTTCAT

Product: DNA polymerase III subunits gamma and tau

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 522; Mature: 522

Protein sequence:

>522_residues
MMRSEQALYRRYRPEVFSEMVGQDGVTGLLSSALDNNRLGHAYLFSGPRGCGKTTSARVFARCLNCVNGPTSSPCGKCQS
CIDLGRSGNGSLDVIEIDAASHNGVDDARALRERAIFAPAQSRFRIFILDEAHMVTTQGFNALLKIVEEPPAHVKFIFAT
TEPEKVLGTIRSRTFSYPFALVSASIMQPYIADICNKEKIRVEDGVLPLVVRAGGGSVRDTLSILDQLMVGKCITYESTV
GLLRLAPDILLYDLFLAIAEDDGATAFASIEKVVEAGYEPVQFIEDLMSWVKDMTLAVCNAEGVFDSYAPEKVERIFALS
KRFSLKDLDAMAEILFSRSNRLSGPISPRLLLEIMLASLLLAVGKSKLQVSDRVSDTQRQKGYPGNLLHNWSRVLDALKS
ETANMLQHSKPLRLTGSSLTIGMPQEILSRFVANKAAIDDLKSVLYKITGVQLRLSGKPIDDPDNTVKTPSSCLDNGTVT
DNSGYKNTEVDDAETKEKPDNPITGEAVLVQAGAKLISEEEQ

Sequences:

>Translated_522_residues
MMRSEQALYRRYRPEVFSEMVGQDGVTGLLSSALDNNRLGHAYLFSGPRGCGKTTSARVFARCLNCVNGPTSSPCGKCQS
CIDLGRSGNGSLDVIEIDAASHNGVDDARALRERAIFAPAQSRFRIFILDEAHMVTTQGFNALLKIVEEPPAHVKFIFAT
TEPEKVLGTIRSRTFSYPFALVSASIMQPYIADICNKEKIRVEDGVLPLVVRAGGGSVRDTLSILDQLMVGKCITYESTV
GLLRLAPDILLYDLFLAIAEDDGATAFASIEKVVEAGYEPVQFIEDLMSWVKDMTLAVCNAEGVFDSYAPEKVERIFALS
KRFSLKDLDAMAEILFSRSNRLSGPISPRLLLEIMLASLLLAVGKSKLQVSDRVSDTQRQKGYPGNLLHNWSRVLDALKS
ETANMLQHSKPLRLTGSSLTIGMPQEILSRFVANKAAIDDLKSVLYKITGVQLRLSGKPIDDPDNTVKTPSSCLDNGTVT
DNSGYKNTEVDDAETKEKPDNPITGEAVLVQAGAKLISEEEQ
>Mature_522_residues
MMRSEQALYRRYRPEVFSEMVGQDGVTGLLSSALDNNRLGHAYLFSGPRGCGKTTSARVFARCLNCVNGPTSSPCGKCQS
CIDLGRSGNGSLDVIEIDAASHNGVDDARALRERAIFAPAQSRFRIFILDEAHMVTTQGFNALLKIVEEPPAHVKFIFAT
TEPEKVLGTIRSRTFSYPFALVSASIMQPYIADICNKEKIRVEDGVLPLVVRAGGGSVRDTLSILDQLMVGKCITYESTV
GLLRLAPDILLYDLFLAIAEDDGATAFASIEKVVEAGYEPVQFIEDLMSWVKDMTLAVCNAEGVFDSYAPEKVERIFALS
KRFSLKDLDAMAEILFSRSNRLSGPISPRLLLEIMLASLLLAVGKSKLQVSDRVSDTQRQKGYPGNLLHNWSRVLDALKS
ETANMLQHSKPLRLTGSSLTIGMPQEILSRFVANKAAIDDLKSVLYKITGVQLRLSGKPIDDPDNTVKTPSSCLDNGTVT
DNSGYKNTEVDDAETKEKPDNPITGEAVLVQAGAKLISEEEQ

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG2812

COG function: function code L; DNA polymerase III, gamma/tau subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4506491, Length=299, Percent_Identity=24.7491638795987, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI31881687, Length=299, Percent_Identity=24.7491638795987, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI6677723, Length=220, Percent_Identity=23.6363636363636, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI194306571, Length=223, Percent_Identity=23.3183856502242, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI194306567, Length=223, Percent_Identity=23.3183856502242, Blast_Score=74, Evalue=4e-13,
Organism=Escherichia coli, GI1786676, Length=280, Percent_Identity=40.3571428571429, Blast_Score=212, Evalue=6e-56,
Organism=Caenorhabditis elegans, GI17541988, Length=224, Percent_Identity=26.3392857142857, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI71986063, Length=234, Percent_Identity=25.6410256410256, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17554730, Length=218, Percent_Identity=25.6880733944954, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324039, Length=312, Percent_Identity=25.9615384615385, Blast_Score=81, Evalue=3e-16,
Organism=Drosophila melanogaster, GI17647857, Length=372, Percent_Identity=25.2688172043011, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19921076, Length=221, Percent_Identity=26.2443438914027, Blast_Score=69, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003959
- InterPro:   IPR008921
- InterPro:   IPR022754
- InterPro:   IPR012763 [H]

Pfam domain/function: PF00004 AAA; PF12169 DNA_pol3_gamma3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 57025; Mature: 57025

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRSEQALYRRYRPEVFSEMVGQDGVTGLLSSALDNNRLGHAYLFSGPRGCGKTTSARVF
CCCCHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHH
ARCLNCVNGPTSSPCGKCQSCIDLGRSGNGSLDVIEIDAASHNGVDDARALRERAIFAPA
HHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCC
QSRFRIFILDEAHMVTTQGFNALLKIVEEPPAHVKFIFATTEPEKVLGTIRSRTFSYPFA
CCCEEEEEEECCCEEHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHCCCHHH
LVSASIMQPYIADICNKEKIRVEDGVLPLVVRAGGGSVRDTLSILDQLMVGKCITYESTV
HHHHHHHHHHHHHHCCCCCEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHH
GLLRLAPDILLYDLFLAIAEDDGATAFASIEKVVEAGYEPVQFIEDLMSWVKDMTLAVCN
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
AEGVFDSYAPEKVERIFALSKRFSLKDLDAMAEILFSRSNRLSGPISPRLLLEIMLASLL
CCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
LAVGKSKLQVSDRVSDTQRQKGYPGNLLHNWSRVLDALKSETANMLQHSKPLRLTGSSLT
HHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCEEE
IGMPQEILSRFVANKAAIDDLKSVLYKITGVQLRLSGKPIDDPDNTVKTPSSCLDNGTVT
ECCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCHHHHCCCCCC
DNSGYKNTEVDDAETKEKPDNPITGEAVLVQAGAKLISEEEQ
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MMRSEQALYRRYRPEVFSEMVGQDGVTGLLSSALDNNRLGHAYLFSGPRGCGKTTSARVF
CCCCHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHH
ARCLNCVNGPTSSPCGKCQSCIDLGRSGNGSLDVIEIDAASHNGVDDARALRERAIFAPA
HHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCC
QSRFRIFILDEAHMVTTQGFNALLKIVEEPPAHVKFIFATTEPEKVLGTIRSRTFSYPFA
CCCEEEEEEECCCEEHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHCCCHHH
LVSASIMQPYIADICNKEKIRVEDGVLPLVVRAGGGSVRDTLSILDQLMVGKCITYESTV
HHHHHHHHHHHHHHCCCCCEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHH
GLLRLAPDILLYDLFLAIAEDDGATAFASIEKVVEAGYEPVQFIEDLMSWVKDMTLAVCN
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
AEGVFDSYAPEKVERIFALSKRFSLKDLDAMAEILFSRSNRLSGPISPRLLLEIMLASLL
CCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
LAVGKSKLQVSDRVSDTQRQKGYPGNLLHNWSRVLDALKSETANMLQHSKPLRLTGSSLT
HHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCEEE
IGMPQEILSRFVANKAAIDDLKSVLYKITGVQLRLSGKPIDDPDNTVKTPSSCLDNGTVT
ECCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCHHHHCCCCCC
DNSGYKNTEVDDAETKEKPDNPITGEAVLVQAGAKLISEEEQ
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]