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The map label for this gene is ispH [H]

Identifier: 28572805

GI number: 28572805

Start: 755714

End: 756655

Strand: Reverse

Name: ispH [H]

Synonym: TW664

Alternate gene names: 28572805

Gene position: 756655-755714 (Counterclockwise)

Preceding gene: 28572808

Following gene: 28572804

Centisome position: 81.72

GC content: 49.89

Gene sequence:

>942_bases
GTGGCTAAGCGGGTTTTGCTTGCCTCCCCCAGGGGTTACTGCGCTGGCGTTGACAGGGCGGTTATAGCTGTTGAAAAAGC
CCTTGAGCGGCATGGCCCTCCGGTTTACGTGCGCAAGCAGATTGTTCACAACGTGCACGTTGTGGGAAGTCTTGAAAAAA
AAGGTGCGATTTTTGTTGATCAGGTGGATCAGATTCCCCCCGGTGCAGTGACAATCTTTAGTGCGCATGGCGTTAGTCCT
GCTGTTGTTCATTCTGCAAATGAGCGGGGGCTACAAGTTATTGATGCGACGTGCCCGCTTGTAACAAAGGTTCATCGTGA
AGTTATTCGTTTTTCAAAGGCCGGTTATTTCATCCTGCTCATAGGTCATTCTGGTCACGAGGAGGTTGAAGGCACAAGCG
GACATGCCCCAGATAGAGTGTTGGTTGTAAATTCCCCAGATGAGGCAGATACAATCGACGTTCCGCACACGGATAAGTTG
GTTTGGCTGAGTCAAACAACCCTATCTGTTGACGAAACCTTAGAGACAGTTAAGCGCTTGCGCCTACGCTTCCCTCACAT
ACAAGACCCACCGAGTGATGATATATGCTATGCAACACAGAATAGGCAAGCGGCTGTCAAAAAAATAGCACCGCTGTCTG
ACTTGGTGATTGTTGTTGGTTCTGCTAACTCGTCAAACTCAGTTAGACTTGTTGAGGTGGCGCTCGAAAATGGCGCCAAG
GCTGCTGTCCGCCTTGATTATGCCGACGAGCTTGATCCGGCTTTGCTGGATAAGGTTGATGTTGTTGGTGTCACTAGTGG
CGCGTCCGTGCCGGAGATTTTGGTTAGGGATTTGCTAGAAGTCCTGGCACGCGCAGGATATACGAAGGTAGAGTCTGTAC
AGACAGCGGTCGAGGATTTGGTCTTTTCCCTACCCAAGGGTCTTAGAGAAAGTAGAAAATGA

Upstream 100 bases:

>100_bases
GTGTTTGCTATATGTTTGTGCTCTGGGTCCTATCTTAATCGGGTTGTATTGTTTAGCTGCTTGACTTGTTTCTGTGCATC
ATACAGGTTTGGGTTCCGGC

Downstream 100 bases:

>100_bases
GCAAAGCCGATCTTAAGAAAGCTCCGAAATATGGTGAATACGCGACTTTTGAGCAGCAGCAGGCGCTTATACAGACTTCA
ACGGAGTTACCGAAGAAAAG

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MAKRVLLASPRGYCAGVDRAVIAVEKALERHGPPVYVRKQIVHNVHVVGSLEKKGAIFVDQVDQIPPGAVTIFSAHGVSP
AVVHSANERGLQVIDATCPLVTKVHREVIRFSKAGYFILLIGHSGHEEVEGTSGHAPDRVLVVNSPDEADTIDVPHTDKL
VWLSQTTLSVDETLETVKRLRLRFPHIQDPPSDDICYATQNRQAAVKKIAPLSDLVIVVGSANSSNSVRLVEVALENGAK
AAVRLDYADELDPALLDKVDVVGVTSGASVPEILVRDLLEVLARAGYTKVESVQTAVEDLVFSLPKGLRESRK

Sequences:

>Translated_313_residues
MAKRVLLASPRGYCAGVDRAVIAVEKALERHGPPVYVRKQIVHNVHVVGSLEKKGAIFVDQVDQIPPGAVTIFSAHGVSP
AVVHSANERGLQVIDATCPLVTKVHREVIRFSKAGYFILLIGHSGHEEVEGTSGHAPDRVLVVNSPDEADTIDVPHTDKL
VWLSQTTLSVDETLETVKRLRLRFPHIQDPPSDDICYATQNRQAAVKKIAPLSDLVIVVGSANSSNSVRLVEVALENGAK
AAVRLDYADELDPALLDKVDVVGVTSGASVPEILVRDLLEVLARAGYTKVESVQTAVEDLVFSLPKGLRESRK
>Mature_312_residues
AKRVLLASPRGYCAGVDRAVIAVEKALERHGPPVYVRKQIVHNVHVVGSLEKKGAIFVDQVDQIPPGAVTIFSAHGVSPA
VVHSANERGLQVIDATCPLVTKVHREVIRFSKAGYFILLIGHSGHEEVEGTSGHAPDRVLVVNSPDEADTIDVPHTDKLV
WLSQTTLSVDETLETVKRLRLRFPHIQDPPSDDICYATQNRQAAVKKIAPLSDLVIVVGSANSSNSVRLVEVALENGAKA
AVRLDYADELDPALLDKVDVVGVTSGASVPEILVRDLLEVLARAGYTKVESVQTAVEDLVFSLPKGLRESRK

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) [H]

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family [H]

Homologues:

Organism=Escherichia coli, GI1786212, Length=309, Percent_Identity=49.5145631067961, Blast_Score=293, Evalue=1e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003451 [H]

Pfam domain/function: PF02401 LYTB [H]

EC number: =1.17.1.2 [H]

Molecular weight: Translated: 33806; Mature: 33675

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKRVLLASPRGYCAGVDRAVIAVEKALERHGPPVYVRKQIVHNVHVVGSLEKKGAIFVD
CCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHHEEECCCCCCCEEEE
QVDQIPPGAVTIFSAHGVSPAVVHSANERGLQVIDATCPLVTKVHREVIRFSKAGYFILL
HHCCCCCCEEEEEECCCCCHHEEECCCCCCCEEEECCCHHHHHHHHHHHHHCCCCEEEEE
IGHSGHEEVEGTSGHAPDRVLVVNSPDEADTIDVPHTDKLVWLSQTTLSVDETLETVKRL
EECCCCCCCCCCCCCCCCEEEEEECCCCCCEECCCCCCCEEEEECCCCCHHHHHHHHHHH
RLRFPHIQDPPSDDICYATQNRQAAVKKIAPLSDLVIVVGSANSSNSVRLVEVALENGAK
HHCCCCCCCCCCCCEEEECCCCHHHHHHHCCCCCEEEEEECCCCCCCEEEEEEEECCCCC
AAVRLDYADELDPALLDKVDVVGVTSGASVPEILVRDLLEVLARAGYTKVESVQTAVEDL
EEEEECCHHCCCHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
VFSLPKGLRESRK
HHHHHHHHHHCCC
>Mature Secondary Structure 
AKRVLLASPRGYCAGVDRAVIAVEKALERHGPPVYVRKQIVHNVHVVGSLEKKGAIFVD
CCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHHEEECCCCCCCEEEE
QVDQIPPGAVTIFSAHGVSPAVVHSANERGLQVIDATCPLVTKVHREVIRFSKAGYFILL
HHCCCCCCEEEEEECCCCCHHEEECCCCCCCEEEECCCHHHHHHHHHHHHHCCCCEEEEE
IGHSGHEEVEGTSGHAPDRVLVVNSPDEADTIDVPHTDKLVWLSQTTLSVDETLETVKRL
EECCCCCCCCCCCCCCCCEEEEEECCCCCCEECCCCCCCEEEEECCCCCHHHHHHHHHHH
RLRFPHIQDPPSDDICYATQNRQAAVKKIAPLSDLVIVVGSANSSNSVRLVEVALENGAK
HHCCCCCCCCCCCCEEEECCCCHHHHHHHCCCCCEEEEEECCCCCCCEEEEEEEECCCCC
AAVRLDYADELDPALLDKVDVVGVTSGASVPEILVRDLLEVLARAGYTKVESVQTAVEDL
EEEEECCHHCCCHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
VFSLPKGLRESRK
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12902375 [H]