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The map label for this gene is pheS

Identifier: 28572752

GI number: 28572752

Start: 687857

End: 688867

Strand: Reverse

Name: pheS

Synonym: TW605

Alternate gene names: 28572752

Gene position: 688867-687857 (Counterclockwise)

Preceding gene: 28572753

Following gene: 28572751

Centisome position: 74.4

GC content: 46.88

Gene sequence:

>1011_bases
GTGGATGAAATACAGGACATAGAGGGTCTGACACGCCGTGCGCTTCAGGCAATCTCCCGTATTGACACTCTTCGTGATTT
GACGAAACTGAAGAATGACAATCTTGGGTCTACATCTTGGCTTGCGAAATACAGTGCGAGTATAAAAATCCTCTCGCAAG
AGAAAAAGCCCATTATCGGTAAAGCTGTCTCTGAGGCACGCCGGAAAATACTAGAAGCCTGTCAAGACAGAGATCTAGCC
CTTCGACTGAATGCGCAGAATGAACAATTTACCCGTGAAACACTCGACATTACAGCTCTTCCAACACGAATCTTTCCAGG
CGCACGCCATCCAATACATGTTTTACAGGACAAGATATTGGATTTTTTTCTAATGCGTGGATGGAGTGTTGTGGAAGGTC
CAGAGCTTGAAAGTGAATGGTTGAATTTTGATGCCCTTAACATAGGGCCCTTTCACCCCGCACGCGAGGAAAGCGATACG
ATCTTTGCTGAACCCCGATCCGCTTCTATGCTTCTTAGAACACACACATCACCTGTTCAACTTCGTGCGCTTGTCTCAAA
CCCATTACCGCTGTATTGCGTCAGCAGTGGCAAGGTTTTTCGCTCTGACCCACTTGATGCAACACATACTCCGGTCTTCC
ATCAGCTTGAAGGGCTGGTATGCGACAGGAATATAACTCTTGGGCACCTTAAAGGTACGGTTGAGGATCTTGCGCGGTAT
CTCTTTGGGGCTGAGGTGAACTTACGAATGCGATGTAATTATTTTCCATTTACCGAGCCAAGCGCAGAGTTTGATATTTC
TAGGGATGGCATTGACTGGACCGAGTGGGGTGGCTGTGGGCTTGTAAATTCGAAAGTTCTCTCAATGGCTGGTGTTGACA
CGGTACATTACACCGGTTTTGCCTTTGGCTTTGGCCTTGAGAGGACTCTGCAGTTCATACACTCGTTATCTGATATGCGT
GACATAGTTGAGGGAGATATTCGATTCTCACAGCAATTTGGTCTCAAGTAG

Upstream 100 bases:

>100_bases
TTAGGGTTTTATTTACCGTTCACTTCCGGTGTGTGCTGCATCTCACGGTTCGTATGATATTTCCTGCATAATGTTGTATA
GCTGAGGAAAACTAATTAAA

Downstream 100 bases:

>100_bases
ACTGTGTTGCGGCAGTGTTGTCAGGCGCCCTGCTGCGACGAGGGATTGTTCCTGGAGCATTTCCAGGTATTAGGAGGATG
GTATGGCGGTCCTTAGGGTT

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MDEIQDIEGLTRRALQAISRIDTLRDLTKLKNDNLGSTSWLAKYSASIKILSQEKKPIIGKAVSEARRKILEACQDRDLA
LRLNAQNEQFTRETLDITALPTRIFPGARHPIHVLQDKILDFFLMRGWSVVEGPELESEWLNFDALNIGPFHPAREESDT
IFAEPRSASMLLRTHTSPVQLRALVSNPLPLYCVSSGKVFRSDPLDATHTPVFHQLEGLVCDRNITLGHLKGTVEDLARY
LFGAEVNLRMRCNYFPFTEPSAEFDISRDGIDWTEWGGCGLVNSKVLSMAGVDTVHYTGFAFGFGLERTLQFIHSLSDMR
DIVEGDIRFSQQFGLK

Sequences:

>Translated_336_residues
MDEIQDIEGLTRRALQAISRIDTLRDLTKLKNDNLGSTSWLAKYSASIKILSQEKKPIIGKAVSEARRKILEACQDRDLA
LRLNAQNEQFTRETLDITALPTRIFPGARHPIHVLQDKILDFFLMRGWSVVEGPELESEWLNFDALNIGPFHPAREESDT
IFAEPRSASMLLRTHTSPVQLRALVSNPLPLYCVSSGKVFRSDPLDATHTPVFHQLEGLVCDRNITLGHLKGTVEDLARY
LFGAEVNLRMRCNYFPFTEPSAEFDISRDGIDWTEWGGCGLVNSKVLSMAGVDTVHYTGFAFGFGLERTLQFIHSLSDMR
DIVEGDIRFSQQFGLK
>Mature_336_residues
MDEIQDIEGLTRRALQAISRIDTLRDLTKLKNDNLGSTSWLAKYSASIKILSQEKKPIIGKAVSEARRKILEACQDRDLA
LRLNAQNEQFTRETLDITALPTRIFPGARHPIHVLQDKILDFFLMRGWSVVEGPELESEWLNFDALNIGPFHPAREESDT
IFAEPRSASMLLRTHTSPVQLRALVSNPLPLYCVSSGKVFRSDPLDATHTPVFHQLEGLVCDRNITLGHLKGTVEDLARY
LFGAEVNLRMRCNYFPFTEPSAEFDISRDGIDWTEWGGCGLVNSKVLSMAGVDTVHYTGFAFGFGLERTLQFIHSLSDMR
DIVEGDIRFSQQFGLK

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5729820, Length=291, Percent_Identity=27.8350515463918, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI4758340, Length=262, Percent_Identity=31.2977099236641, Blast_Score=101, Evalue=1e-21,
Organism=Escherichia coli, GI1788007, Length=324, Percent_Identity=38.8888888888889, Blast_Score=241, Evalue=5e-65,
Organism=Caenorhabditis elegans, GI32563657, Length=305, Percent_Identity=27.8688524590164, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17508957, Length=305, Percent_Identity=27.8688524590164, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI32566635, Length=288, Percent_Identity=29.5138888888889, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6321087, Length=263, Percent_Identity=26.9961977186312, Blast_Score=100, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6325304, Length=272, Percent_Identity=27.2058823529412, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI18858079, Length=273, Percent_Identity=31.1355311355311, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI17137424, Length=285, Percent_Identity=28.4210526315789, Blast_Score=91, Evalue=9e-19,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_TROW8 (Q83HH3)

Other databases:

- EMBL:   BX251412
- RefSeq:   NP_789532.1
- ProteinModelPortal:   Q83HH3
- SMR:   Q83HH3
- STRING:   Q83HH3
- GeneID:   1064811
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW605
- eggNOG:   COG0016
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- ProtClustDB:   PRK00488
- BioCyc:   TWHI218496:TW0577-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 37926; Mature: 37926

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDEIQDIEGLTRRALQAISRIDTLRDLTKLKNDNLGSTSWLAKYSASIKILSQEKKPIIG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCEEEEEECCCCCHHH
KAVSEARRKILEACQDRDLALRLNAQNEQFTRETLDITALPTRIFPGARHPIHVLQDKIL
HHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHEEEECCHHHCCCCCCHHHHHHHHHH
DFFLMRGWSVVEGPELESEWLNFDALNIGPFHPAREESDTIFAEPRSASMLLRTHTSPVQ
HHHHHCCCCCCCCCCCHHHHCCCCEEECCCCCCCCCCCCEEEECCCCCEEEEEECCCHHH
LRALVSNPLPLYCVSSGKVFRSDPLDATHTPVFHQLEGLVCDRNITLGHLKGTVEDLARY
HHHHHCCCCCEEEECCCCEEECCCCCCCCCCHHHHHHCEEECCCCEEHHHHCCHHHHHHH
LFGAEVNLRMRCNYFPFTEPSAEFDISRDGIDWTEWGGCGLVNSKVLSMAGVDTVHYTGF
HHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEECHH
AFGFGLERTLQFIHSLSDMRDIVEGDIRFSQQFGLK
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDEIQDIEGLTRRALQAISRIDTLRDLTKLKNDNLGSTSWLAKYSASIKILSQEKKPIIG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCEEEEEECCCCCHHH
KAVSEARRKILEACQDRDLALRLNAQNEQFTRETLDITALPTRIFPGARHPIHVLQDKIL
HHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHEEEECCHHHCCCCCCHHHHHHHHHH
DFFLMRGWSVVEGPELESEWLNFDALNIGPFHPAREESDTIFAEPRSASMLLRTHTSPVQ
HHHHHCCCCCCCCCCCHHHHCCCCEEECCCCCCCCCCCCEEEECCCCCEEEEEECCCHHH
LRALVSNPLPLYCVSSGKVFRSDPLDATHTPVFHQLEGLVCDRNITLGHLKGTVEDLARY
HHHHHCCCCCEEEECCCCEEECCCCCCCCCCHHHHHHCEEECCCCEEHHHHCCHHHHHHH
LFGAEVNLRMRCNYFPFTEPSAEFDISRDGIDWTEWGGCGLVNSKVLSMAGVDTVHYTGF
HHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEECHH
AFGFGLERTLQFIHSLSDMRDIVEGDIRFSQQFGLK
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12606174