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The map label for this gene is glk [H]

Identifier: 28572706

GI number: 28572706

Start: 594860

End: 595777

Strand: Reverse

Name: glk [H]

Synonym: TW558

Alternate gene names: 28572706

Gene position: 595777-594860 (Counterclockwise)

Preceding gene: 28572707

Following gene: 28572705

Centisome position: 64.34

GC content: 46.19

Gene sequence:

>918_bases
TTGGTGCGTGCAATCGGCGTTGATATCGGAGGGACAAAGATATCGGCGGCATTGGTTGATAGATCTGGCAATATGGACGG
CCAGCTTACTGTGCCGACAGATCCCGTTGGTGTTATTGATCAGGTCGTGGAGCTTGTCAATAAACTGTCTGATGGTGAAT
CGGTGTGTGCAGTTGGTGTTGCTGTTGCCAGTTTCCTAAATTATAGTCGTGATTTTGTTTATAATTCTCCTAATTTGGGC
CTGGAAAATATTCCGATACGCAACCTTTTGCAAAACAGAATTAAATTTCCCGTTTTTATAGAGAATGATGCAAATGCCGC
CGCGTGGGCTGAGTGGCGTTTTGCTCCGCGATCTGTGGAGATTGTTAATTCACAAGATTTGTTTATGATTACTGTTGGTA
CCGGTGTGGGTGGAGCGGCAATTTTAGACGGCAAGATCCTTAAGGGGGGTTTTGGTATCGCTTCGGAGCCGGGGCATATT
GTTCTTGTGCCAGACGGAATTCCGTGTGGTTGTGGCAAACGTGGCTGCAGTGAACAGTATGCTTCTGGGACTGCGCTTTT
GCGTTATGTAAAGAGCAATATGCCAGATTTTCCGTTTGGTAGGAACCCGTCTGATGTAATTGGCGAGCTTGTTGCCAAGT
CGGATCCAGTTGTTCTTTCTGCCGTTTCTCAAGTCGCAGATAACGTGGGTCGCACAGCAGCTGCTGTTACCGCTATTTTG
GACCCTGAGTTGTTTGTTATCGGTGGTGGGGTCAGTAAAATTGGCACCTTTTTCATTGATTCAATAAGAGAGTCTTATAA
AGAATATTTACCCCATTCAGAGTCACGGCCGTGTGCTAATTTTCGATTGGCCCATTTTTACAATACTGCTGGGATTATAG
GCGCAGCAGACTTGGCAAGGTCTTCTCTTGCTACTTGA

Upstream 100 bases:

>100_bases
TTGTCGACGGTGATCCGCTTTATTACCTCGCTCGTGTTGTTCCCTAACCGGACTATTTGCCTGTTCGTGCTTTTTGTATT
TTGTATTTCTGAGAGCGGTT

Downstream 100 bases:

>100_bases
AAATTTCCAAGGTTATTGTTTTTCAAAGTCATTGTGCCGCCTTTGTGACTCTCTTTGTGACTCTTAGGGGGGCTTGTGTG
TATTTCCTGATGAAGCTTCT

Product: glucokinase

Products: NA

Alternate protein names: Glucose kinase [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MVRAIGVDIGGTKISAALVDRSGNMDGQLTVPTDPVGVIDQVVELVNKLSDGESVCAVGVAVASFLNYSRDFVYNSPNLG
LENIPIRNLLQNRIKFPVFIENDANAAAWAEWRFAPRSVEIVNSQDLFMITVGTGVGGAAILDGKILKGGFGIASEPGHI
VLVPDGIPCGCGKRGCSEQYASGTALLRYVKSNMPDFPFGRNPSDVIGELVAKSDPVVLSAVSQVADNVGRTAAAVTAIL
DPELFVIGGGVSKIGTFFIDSIRESYKEYLPHSESRPCANFRLAHFYNTAGIIGAADLARSSLAT

Sequences:

>Translated_305_residues
MVRAIGVDIGGTKISAALVDRSGNMDGQLTVPTDPVGVIDQVVELVNKLSDGESVCAVGVAVASFLNYSRDFVYNSPNLG
LENIPIRNLLQNRIKFPVFIENDANAAAWAEWRFAPRSVEIVNSQDLFMITVGTGVGGAAILDGKILKGGFGIASEPGHI
VLVPDGIPCGCGKRGCSEQYASGTALLRYVKSNMPDFPFGRNPSDVIGELVAKSDPVVLSAVSQVADNVGRTAAAVTAIL
DPELFVIGGGVSKIGTFFIDSIRESYKEYLPHSESRPCANFRLAHFYNTAGIIGAADLARSSLAT
>Mature_305_residues
MVRAIGVDIGGTKISAALVDRSGNMDGQLTVPTDPVGVIDQVVELVNKLSDGESVCAVGVAVASFLNYSRDFVYNSPNLG
LENIPIRNLLQNRIKFPVFIENDANAAAWAEWRFAPRSVEIVNSQDLFMITVGTGVGGAAILDGKILKGGFGIASEPGHI
VLVPDGIPCGCGKRGCSEQYASGTALLRYVKSNMPDFPFGRNPSDVIGELVAKSDPVVLSAVSQVADNVGRTAAAVTAIL
DPELFVIGGGVSKIGTFFIDSIRESYKEYLPHSESRPCANFRLAHFYNTAGIIGAADLARSSLAT

Specific function: Required for glucose repression of many different genes [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Homo sapiens, GI298566315, Length=198, Percent_Identity=29.7979797979798, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI298566325, Length=198, Percent_Identity=29.7979797979798, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI190014632, Length=198, Percent_Identity=29.7979797979798, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI4885285, Length=198, Percent_Identity=29.7979797979798, Blast_Score=72, Evalue=6e-13,
Organism=Escherichia coli, GI87081733, Length=311, Percent_Identity=27.9742765273312, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1787363, Length=265, Percent_Identity=29.0566037735849, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI87082230, Length=272, Percent_Identity=28.6764705882353, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790522, Length=307, Percent_Identity=25.7328990228013, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1786891, Length=273, Percent_Identity=23.0769230769231, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787878, Length=279, Percent_Identity=22.2222222222222, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600
- InterPro:   IPR004654 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.2 [H]

Molecular weight: Translated: 32177; Mature: 32177

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRAIGVDIGGTKISAALVDRSGNMDGQLTVPTDPVGVIDQVVELVNKLSDGESVCAVGV
CEEEEEECCCCCEEEEEEEECCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHH
AVASFLNYSRDFVYNSPNLGLENIPIRNLLQNRIKFPVFIENDANAAAWAEWRFAPRSVE
HHHHHHHCCHHEEECCCCCCCCCCCHHHHHHHHCCCCEEEECCCCCCEEHEEECCCCEEE
IVNSQDLFMITVGTGVGGAAILDGKILKGGFGIASEPGHIVLVPDGIPCGCGKRGCSEQY
EECCCCEEEEEECCCCCCEEEECCEEEECCCCCCCCCCEEEEEECCCCCCCCCCCCCHHH
ASGTALLRYVKSNMPDFPFGRNPSDVIGELVAKSDPVVLSAVSQVADNVGRTAAAVTAIL
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHC
DPELFVIGGGVSKIGTFFIDSIRESYKEYLPHSESRPCANFRLAHFYNTAGIIGAADLAR
CCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCHHHHHHHH
SSLAT
HHCCC
>Mature Secondary Structure
MVRAIGVDIGGTKISAALVDRSGNMDGQLTVPTDPVGVIDQVVELVNKLSDGESVCAVGV
CEEEEEECCCCCEEEEEEEECCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHH
AVASFLNYSRDFVYNSPNLGLENIPIRNLLQNRIKFPVFIENDANAAAWAEWRFAPRSVE
HHHHHHHCCHHEEECCCCCCCCCCCHHHHHHHHCCCCEEEECCCCCCEEHEEECCCCEEE
IVNSQDLFMITVGTGVGGAAILDGKILKGGFGIASEPGHIVLVPDGIPCGCGKRGCSEQY
EECCCCEEEEEECCCCCCEEEECCEEEECCCCCCCCCCEEEEEECCCCCCCCCCCCCHHH
ASGTALLRYVKSNMPDFPFGRNPSDVIGELVAKSDPVVLSAVSQVADNVGRTAAAVTAIL
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHC
DPELFVIGGGVSKIGTFFIDSIRESYKEYLPHSESRPCANFRLAHFYNTAGIIGAADLAR
CCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCHHHHHHHH
SSLAT
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1435260; 12000953 [H]