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The map label for this gene is murF [H]

Identifier: 28572695

GI number: 28572695

Start: 583384

End: 584778

Strand: Reverse

Name: murF [H]

Synonym: TW547

Alternate gene names: 28572695

Gene position: 584778-583384 (Counterclockwise)

Preceding gene: 28572696

Following gene: 28572694

Centisome position: 63.16

GC content: 43.37

Gene sequence:

>1395_bases
ATGCTAATCAGGCGTGCCGATATTCAACGCGTGACCGGCGGTATTTTGCACAACACCTCTTTATCGGAAAGTTTGTTGCC
AATTAACGGGCGCCTGAAGATAGATTCAAGGAATATTTCACAGGGCGATATTTTTGTTGCTCACACTGGCACAAAATTCG
ACGGGCATGAGTTTTTATATCAGGCAAAGCAGTCTGGTGCAAGCCTTGCAATTGTGACGCGTGTACAGGAAATTGACCTT
CCACAGTTACTTGTTGGCAATGTCACAGAAGCCATGCATGCAATTGCAGGTTTTAAACTATGTCTCAGCAGAGATACGAT
TACGGTGATTGGGATCACGGGGTCGAATGGTAAGACAACAACCAAGAGTGTTCTCGCATATGTTCTGTCAAAATTTGGTA
ATACTGTGGCAACTCATGGTAATCAGAATAATGAAATAGGTTTTCCAATCACTGTTTCCAGAATTGAGCCTTTAACAAAA
TATCTTGTCCTTGAATACGGTGCATTTAAGCCTGGTGATATAGATTGTTTAAAGAAGATCGCTGTGCCCGATATTGCAGT
TCTCTTATCAGCAGGCCATGCTCATGTTGAGAAAAGTGGCAGTTTAGAAAGTGTCTGTTCAATGAAAGCTGAGCTTGCGC
GCGACCTGCCAGAGGGATCAAAGGTATTGCTCAATTATGATGACCCGAGGATTGCCAGGCTCTCTGTTGGAAATCGATAT
TATTTTGGAACGGATAAGCGCGCGCATTTTCGGGCGAGTCACGTAGAAACAAATATAATCAAGACGCGTTTCATTGTTAC
TTATCAAAAGGGTAGCGTGGAGGTTGAGACTAAACTGATAGGTAAACACCAGGTCAGTAATGTTCTGGCTGCCCTGTCGA
CTCTTGTGCTTTTGGGATTTGACGCTGAGGTGTGCGCACGGTATATATCAGAGGTTAACCCGATTGATGGGCGTATGCAA
TATATTCCTCTCGGAGACTTTTCCATTATTCATGATGCCTACAATGCGTCCTTTGAATCGGTGAAGTGCGCTCTTGACTC
GCTTGTTGTTTTGGGGTCTCTAGCCAAACGGCGTATTGCTGTTCTTGGGCGAATACATGATATGGGGGAGCTGGCACAAG
ATGTGTATAGGCAGCTTGTCAAACAGTTTTTGGACACAGACATTAATCTTCTGGTTATTGTTTCAGAGAAACAGATGTAT
CTCATAGCTAAGGAATTGGCCAAAGGAGTGCCAAATAAGGAGATTGTGTGGTTTGAAGATCTGGAAACAGCAAAACATGA
GATATTTGATTTATTTTCTCCAGGAGATATAGTTCTTTTCAAAGCCTCAAATGCGGAGAATTTTTCTAGCCTCGTGGAGA
GTGTAAAGCACAAAGCAGGCAAGTTTGCGGGATGA

Upstream 100 bases:

>100_bases
AAAGGTTATGAGTCATGTTTTAAAAAAGTATCGAGTTTTGCCATCTACAACATTGCCCACAGAATTAGCGCTGCGGTAAA
CCTTTATAGAAAGGCAAGAT

Downstream 100 bases:

>100_bases
TTGCAATTTTGCTTGCCGTTGCATTCGGTATTACATTCACACTGTTTACTACCCCATTTTTTATAAGACTGTTTAGGAAA
ATTGGTTGGGGTCAGTTTAT

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate-- D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MLIRRADIQRVTGGILHNTSLSESLLPINGRLKIDSRNISQGDIFVAHTGTKFDGHEFLYQAKQSGASLAIVTRVQEIDL
PQLLVGNVTEAMHAIAGFKLCLSRDTITVIGITGSNGKTTTKSVLAYVLSKFGNTVATHGNQNNEIGFPITVSRIEPLTK
YLVLEYGAFKPGDIDCLKKIAVPDIAVLLSAGHAHVEKSGSLESVCSMKAELARDLPEGSKVLLNYDDPRIARLSVGNRY
YFGTDKRAHFRASHVETNIIKTRFIVTYQKGSVEVETKLIGKHQVSNVLAALSTLVLLGFDAEVCARYISEVNPIDGRMQ
YIPLGDFSIIHDAYNASFESVKCALDSLVVLGSLAKRRIAVLGRIHDMGELAQDVYRQLVKQFLDTDINLLVIVSEKQMY
LIAKELAKGVPNKEIVWFEDLETAKHEIFDLFSPGDIVLFKASNAENFSSLVESVKHKAGKFAG

Sequences:

>Translated_464_residues
MLIRRADIQRVTGGILHNTSLSESLLPINGRLKIDSRNISQGDIFVAHTGTKFDGHEFLYQAKQSGASLAIVTRVQEIDL
PQLLVGNVTEAMHAIAGFKLCLSRDTITVIGITGSNGKTTTKSVLAYVLSKFGNTVATHGNQNNEIGFPITVSRIEPLTK
YLVLEYGAFKPGDIDCLKKIAVPDIAVLLSAGHAHVEKSGSLESVCSMKAELARDLPEGSKVLLNYDDPRIARLSVGNRY
YFGTDKRAHFRASHVETNIIKTRFIVTYQKGSVEVETKLIGKHQVSNVLAALSTLVLLGFDAEVCARYISEVNPIDGRMQ
YIPLGDFSIIHDAYNASFESVKCALDSLVVLGSLAKRRIAVLGRIHDMGELAQDVYRQLVKQFLDTDINLLVIVSEKQMY
LIAKELAKGVPNKEIVWFEDLETAKHEIFDLFSPGDIVLFKASNAENFSSLVESVKHKAGKFAG
>Mature_464_residues
MLIRRADIQRVTGGILHNTSLSESLLPINGRLKIDSRNISQGDIFVAHTGTKFDGHEFLYQAKQSGASLAIVTRVQEIDL
PQLLVGNVTEAMHAIAGFKLCLSRDTITVIGITGSNGKTTTKSVLAYVLSKFGNTVATHGNQNNEIGFPITVSRIEPLTK
YLVLEYGAFKPGDIDCLKKIAVPDIAVLLSAGHAHVEKSGSLESVCSMKAELARDLPEGSKVLLNYDDPRIARLSVGNRY
YFGTDKRAHFRASHVETNIIKTRFIVTYQKGSVEVETKLIGKHQVSNVLAALSTLVLLGFDAEVCARYISEVNPIDGRMQ
YIPLGDFSIIHDAYNASFESVKCALDSLVVLGSLAKRRIAVLGRIHDMGELAQDVYRQLVKQFLDTDINLLVIVSEKQMY
LIAKELAKGVPNKEIVWFEDLETAKHEIFDLFSPGDIVLFKASNAENFSSLVESVKHKAGKFAG

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=351, Percent_Identity=31.6239316239316, Blast_Score=159, Evalue=5e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 50961; Mature: 50961

Theoretical pI: Translated: 7.97; Mature: 7.97

Prosite motif: PS00589 PTS_HPR_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIRRADIQRVTGGILHNTSLSESLLPINGRLKIDSRNISQGDIFVAHTGTKFDGHEFLY
CCCCCCHHHHHHCCCEECCCCCCCCCCCCCEEEECCCCCCCCCEEEEECCCCCCHHHHHH
QAKQSGASLAIVTRVQEIDLPQLLVGNVTEAMHAIAGFKLCLSRDTITVIGITGSNGKTT
HHHHCCCEEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHEECCCEEEEEEEECCCCCCH
TKSVLAYVLSKFGNTVATHGNQNNEIGFPITVSRIEPLTKYLVLEYGAFKPGDIDCLKKI
HHHHHHHHHHHHCCEEEECCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
AVPDIAVLLSAGHAHVEKSGSLESVCSMKAELARDLPEGSKVLLNYDDPRIARLSVGNRY
CCCHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEECCCEE
YFGTDKRAHFRASHVETNIIKTRFIVTYQKGSVEVETKLIGKHQVSNVLAALSTLVLLGF
EECCCCCCCEEHHHHHHHEEEEEEEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHCC
DAEVCARYISEVNPIDGRMQYIPLGDFSIIHDAYNASFESVKCALDSLVVLGSLAKRRIA
CHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
VLGRIHDMGELAQDVYRQLVKQFLDTDINLLVIVSEKQMYLIAKELAKGVPNKEIVWFED
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCCCEEEEEHH
LETAKHEIFDLFSPGDIVLFKASNAENFSSLVESVKHKAGKFAG
HHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLIRRADIQRVTGGILHNTSLSESLLPINGRLKIDSRNISQGDIFVAHTGTKFDGHEFLY
CCCCCCHHHHHHCCCEECCCCCCCCCCCCCEEEECCCCCCCCCEEEEECCCCCCHHHHHH
QAKQSGASLAIVTRVQEIDLPQLLVGNVTEAMHAIAGFKLCLSRDTITVIGITGSNGKTT
HHHHCCCEEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHEECCCEEEEEEEECCCCCCH
TKSVLAYVLSKFGNTVATHGNQNNEIGFPITVSRIEPLTKYLVLEYGAFKPGDIDCLKKI
HHHHHHHHHHHHCCEEEECCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
AVPDIAVLLSAGHAHVEKSGSLESVCSMKAELARDLPEGSKVLLNYDDPRIARLSVGNRY
CCCHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEECCCEE
YFGTDKRAHFRASHVETNIIKTRFIVTYQKGSVEVETKLIGKHQVSNVLAALSTLVLLGF
EECCCCCCCEEHHHHHHHEEEEEEEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHCC
DAEVCARYISEVNPIDGRMQYIPLGDFSIIHDAYNASFESVKCALDSLVVLGSLAKRRIA
CHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
VLGRIHDMGELAQDVYRQLVKQFLDTDINLLVIVSEKQMYLIAKELAKGVPNKEIVWFED
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCCCEEEEEHH
LETAKHEIFDLFSPGDIVLFKASNAENFSSLVESVKHKAGKFAG
HHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9177243; 12060705 [H]