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The map label for this gene is rnhA

Identifier: 28572680

GI number: 28572680

Start: 569929

End: 570414

Strand: Direct

Name: rnhA

Synonym: TW531

Alternate gene names: 28572680

Gene position: 569929-570414 (Clockwise)

Preceding gene: 28572679

Following gene: 28572687

Centisome position: 61.55

GC content: 47.53

Gene sequence:

>486_bases
ATGACCAATAACGAGATAATTGCAGCAACCGACGGATCATCACTCGCCAACCCGGGGCCATCCGGCTGGGCTTGGTACGT
CGACGAAAATACATGGGACTCTGGTGGATGGGATATTGCAACAAACAATATTGCGGAGCTAACAGCGGTCAGAGAACTTC
TTATCGCTACAAGGCATACCGACAGGCCAATTCTGATTCTGTCAGACAGCAAATATGTTATTAACTCGCTCACAAAATGG
GTTTACTCCTGGAAAATGCGTAAATGGCGTAAAGCAGACGGAAAACCGGTTCTAAATCAAGAAATAATTCAGGAAATAGA
CAGTCTGATGGAAAACCGGAATATAAGAATGAGCTGGGTAAATGCGCACACTGGACACCCTCTCAATGAAGCGGCAGACT
CACTTGCCAGACAGGCTGCAAACAACTTTTCTACTCGGAGCGCTCACATTCCGGGGCCTGGCTGGACAGAACGCTCAGCA
AAATAG

Upstream 100 bases:

>100_bases
AAGCAGAATGGTTGTTATACGCCCCTTGCGCATGCCGAAAACCAGTGTAAAACTGGGCTTAGTATTTGGTAAAAGAAAGG
GCTAACTTACAAAAAACAAA

Downstream 100 bases:

>100_bases
GTATAAGTGATAAATAAACGCCATGGCGTAGCCAGAAGAAACATTACTCTCACAGCCATCAATAAATCGCATAACGGGGC
GACACGGTATAACAGGGCAA

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H

Number of amino acids: Translated: 161; Mature: 160

Protein sequence:

>161_residues
MTNNEIIAATDGSSLANPGPSGWAWYVDENTWDSGGWDIATNNIAELTAVRELLIATRHTDRPILILSDSKYVINSLTKW
VYSWKMRKWRKADGKPVLNQEIIQEIDSLMENRNIRMSWVNAHTGHPLNEAADSLARQAANNFSTRSAHIPGPGWTERSA
K

Sequences:

>Translated_161_residues
MTNNEIIAATDGSSLANPGPSGWAWYVDENTWDSGGWDIATNNIAELTAVRELLIATRHTDRPILILSDSKYVINSLTKW
VYSWKMRKWRKADGKPVLNQEIIQEIDSLMENRNIRMSWVNAHTGHPLNEAADSLARQAANNFSTRSAHIPGPGWTERSA
K
>Mature_160_residues
TNNEIIAATDGSSLANPGPSGWAWYVDENTWDSGGWDIATNNIAELTAVRELLIATRHTDRPILILSDSKYVINSLTKWV
YSWKMRKWRKADGKPVLNQEIIQEIDSLMENRNIRMSWVNAHTGHPLNEAADSLARQAANNFSTRSAHIPGPGWTERSAK

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain

Homologues:

Organism=Escherichia coli, GI1786408, Length=138, Percent_Identity=41.304347826087, Blast_Score=102, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI115532542, Length=151, Percent_Identity=31.1258278145695, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI45552509, Length=152, Percent_Identity=32.2368421052632, Blast_Score=80, Evalue=7e-16,
Organism=Drosophila melanogaster, GI17137476, Length=144, Percent_Identity=34.0277777777778, Blast_Score=80, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH_TROW8 (Q83HK9)

Other databases:

- EMBL:   BX251411
- RefSeq:   NP_789460.1
- ProteinModelPortal:   Q83HK9
- SMR:   Q83HK9
- STRING:   Q83HK9
- GeneID:   1064735
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW531
- eggNOG:   COG0328
- HOGENOM:   HBG742437
- OMA:   WATILRY
- PhylomeDB:   Q83HK9
- ProtClustDB:   PRK06548
- BioCyc:   TWHI218496:TW0505-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00042
- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156

Pfam domain/function: PF00075 RnaseH; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 18086; Mature: 17955

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNNEIIAATDGSSLANPGPSGWAWYVDENTWDSGGWDIATNNIAELTAVRELLIATRHT
CCCCCEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCEECCCCHHHHHHHHHHHHHHCCC
DRPILILSDSKYVINSLTKWVYSWKMRKWRKADGKPVLNQEIIQEIDSLMENRNIRMSWV
CCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEE
NAHTGHPLNEAADSLARQAANNFSTRSAHIPGPGWTERSAK
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TNNEIIAATDGSSLANPGPSGWAWYVDENTWDSGGWDIATNNIAELTAVRELLIATRHT
CCCCEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCEECCCCHHHHHHHHHHHHHHCCC
DRPILILSDSKYVINSLTKWVYSWKMRKWRKADGKPVLNQEIIQEIDSLMENRNIRMSWV
CCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEE
NAHTGHPLNEAADSLARQAANNFSTRSAHIPGPGWTERSAK
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12606174