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The map label for this gene is dapE [H]

Identifier: 28572678

GI number: 28572678

Start: 567920

End: 569260

Strand: Reverse

Name: dapE [H]

Synonym: TW529

Alternate gene names: 28572678

Gene position: 569260-567920 (Counterclockwise)

Preceding gene: 28572681

Following gene: 28572677

Centisome position: 61.48

GC content: 48.77

Gene sequence:

>1341_bases
ATGCATAAGGATGATCTTGATAGAGAGTACTCGCAGGCTCTAAGGGATTTAAAAGAACTCATAAGGTTTTCGACCGTCGC
GTGGCCGGGGTTTGACAGGTCTGTTCTTTTTGAGAGCGCTGAGAGAATTCGCGCGCTTCTTGTTGATACGGACTTTTTTG
ACTGCGTAGAGGTTGTGGATCATTGCGGGATTCCGTCTGTTTTCGCACAGAAGGCACCTGCCCCGGGCTATCCAACTGTC
CTATTGTACGCACACCATGATGTCCAACCCGCAGGTGATCCAGATAAATGGGTTACTCCCGCATTTTCTCCGGACGAGCG
TGATGGCAGGCTGTATGGCAGGGGGGCTGCTGATGATAAGGTCGCCATTGCCATGCACCTCGCGTCTGTGCGGATTTTAA
AGACCCTGAACTCAAAAATAGGTGTTCGGGTGTTTATAGAGGGTGAAGAAGAAATACTTTCTCCAAATTTCCCCAAACTT
CTCAAAAGCAGGAGATCATTTTTCGATGCTGATGTTGCGATTATTGCCGATTCTGGTAATCCGGACGAAGACACACCGGC
TATTACGGTTTCCCTCAGAGGGTCTATTGGGGCAACTGTCCGTGTGACAACCCTTTCTCATGCTGCTCACTCCGGCTTAG
GTGGCGCGGTTCCCGATGCGCTAATGGTTATGATACGTTTATTGAATTCACTGTATGACGATAGTGGGTCTGTCGCAATA
GAGGGTTTGAAAACAACAGATCCTTCATCTGTTCTGACTGATTTTGAACTTACAGAACAAGCCCTGCGCAATGAGGTTGG
TGTTTTGCCGGATGTTAGCCTCGTGGGCAGAGGGTCTATTTCTGAAAGGACGTGGCATGCCCCATCAATCACTATTATCG
GCCTGGATGTGGAGAATGTTGACCTTGCTAGCAATACCCTTCAACCGAGCACTCAGGCCAGGTTGGCATTGCGTATCTCT
CCGGGTCAGGGCACTTTAGAGGCGTCGCGCTTACTTGAACAGCACCTAAGAAATAACACGCCTTTCAATGCGCGCGTTGA
AGTTTTTGATATAACAGCGGGGGAGCCCTATAGAGCAGTTGCCGGTGACAAATACCTTAAGGCAATGCGGTGCGCTATGG
AAGAGGCATGGGGTAAACGCCCAGCCTTGATTGGCATTGGCTGCTCTATTCCGATAGTGTCTATGCTTCAGAGTGAATTT
CCGCAAATGAGTCTTCTTGTTACAGGTGTTGAAGATCCAAAAACTTACGCTCACAGTCCGAATGAGTCATTGAGCCTTAA
AGTGTTCAGAAATGCTATAGCAGCGCAGGTGTATTTTTTGCTGTCACTTGGCGGTGAGTGA

Upstream 100 bases:

>100_bases
TTTCAATAAATAACTATACTTGGGTAAGAAAAATTAGGCAGTAAAAATTAGGCAATGAGCTACTCTATTATTTCGTCCCG
TGTGGATCTAGAATACTTAT

Downstream 100 bases:

>100_bases
TTTGACCAACGGTTCATTGACTGACACCCCCCCCTCCCATGGAGTGCATTTGACAGATGTCGCTGTGAACAAGGTCAAGC
AGTTTCTAGAGCAGGAACAG

Product: hypothetical protein

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MHKDDLDREYSQALRDLKELIRFSTVAWPGFDRSVLFESAERIRALLVDTDFFDCVEVVDHCGIPSVFAQKAPAPGYPTV
LLYAHHDVQPAGDPDKWVTPAFSPDERDGRLYGRGAADDKVAIAMHLASVRILKTLNSKIGVRVFIEGEEEILSPNFPKL
LKSRRSFFDADVAIIADSGNPDEDTPAITVSLRGSIGATVRVTTLSHAAHSGLGGAVPDALMVMIRLLNSLYDDSGSVAI
EGLKTTDPSSVLTDFELTEQALRNEVGVLPDVSLVGRGSISERTWHAPSITIIGLDVENVDLASNTLQPSTQARLALRIS
PGQGTLEASRLLEQHLRNNTPFNARVEVFDITAGEPYRAVAGDKYLKAMRCAMEEAWGKRPALIGIGCSIPIVSMLQSEF
PQMSLLVTGVEDPKTYAHSPNESLSLKVFRNAIAAQVYFLLSLGGE

Sequences:

>Translated_446_residues
MHKDDLDREYSQALRDLKELIRFSTVAWPGFDRSVLFESAERIRALLVDTDFFDCVEVVDHCGIPSVFAQKAPAPGYPTV
LLYAHHDVQPAGDPDKWVTPAFSPDERDGRLYGRGAADDKVAIAMHLASVRILKTLNSKIGVRVFIEGEEEILSPNFPKL
LKSRRSFFDADVAIIADSGNPDEDTPAITVSLRGSIGATVRVTTLSHAAHSGLGGAVPDALMVMIRLLNSLYDDSGSVAI
EGLKTTDPSSVLTDFELTEQALRNEVGVLPDVSLVGRGSISERTWHAPSITIIGLDVENVDLASNTLQPSTQARLALRIS
PGQGTLEASRLLEQHLRNNTPFNARVEVFDITAGEPYRAVAGDKYLKAMRCAMEEAWGKRPALIGIGCSIPIVSMLQSEF
PQMSLLVTGVEDPKTYAHSPNESLSLKVFRNAIAAQVYFLLSLGGE
>Mature_446_residues
MHKDDLDREYSQALRDLKELIRFSTVAWPGFDRSVLFESAERIRALLVDTDFFDCVEVVDHCGIPSVFAQKAPAPGYPTV
LLYAHHDVQPAGDPDKWVTPAFSPDERDGRLYGRGAADDKVAIAMHLASVRILKTLNSKIGVRVFIEGEEEILSPNFPKL
LKSRRSFFDADVAIIADSGNPDEDTPAITVSLRGSIGATVRVTTLSHAAHSGLGGAVPDALMVMIRLLNSLYDDSGSVAI
EGLKTTDPSSVLTDFELTEQALRNEVGVLPDVSLVGRGSISERTWHAPSITIIGLDVENVDLASNTLQPSTQARLALRIS
PGQGTLEASRLLEQHLRNNTPFNARVEVFDITAGEPYRAVAGDKYLKAMRCAMEEAWGKRPALIGIGCSIPIVSMLQSEF
PQMSLLVTGVEDPKTYAHSPNESLSLKVFRNAIAAQVYFLLSLGGE

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Homo sapiens, GI271398239, Length=446, Percent_Identity=26.2331838565022, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI21071039, Length=377, Percent_Identity=27.5862068965517, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI271398277, Length=325, Percent_Identity=25.5384615384615, Blast_Score=77, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI193211059, Length=395, Percent_Identity=26.8354430379747, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17563146, Length=381, Percent_Identity=27.0341207349081, Blast_Score=126, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6321159, Length=441, Percent_Identity=25.8503401360544, Blast_Score=103, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6319758, Length=451, Percent_Identity=22.6164079822616, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24585880, Length=437, Percent_Identity=27.0022883295195, Blast_Score=124, Evalue=9e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 48538; Mature: 48538

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKDDLDREYSQALRDLKELIRFSTVAWPGFDRSVLFESAERIRALLVDTDFFDCVEVVD
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHH
HCGIPSVFAQKAPAPGYPTVLLYAHHDVQPAGDPDKWVTPAFSPDERDGRLYGRGAADDK
HCCCCHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCC
VAIAMHLASVRILKTLNSKIGVRVFIEGEEEILSPNFPKLLKSRRSFFDADVAIIADSGN
EEEHHHHHHHHHHHHHCCCCCEEEEEECCHHHCCCCHHHHHHHHHHHCCCCEEEEECCCC
PDEDTPAITVSLRGSIGATVRVTTLSHAAHSGLGGAVPDALMVMIRLLNSLYDDSGSVAI
CCCCCCEEEEEECCCCCCEEEEEEEHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEE
EGLKTTDPSSVLTDFELTEQALRNEVGVLPDVSLVGRGSISERTWHAPSITIIGLDVENV
EECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCEECCCEEEEEEECCCCC
DLASNTLQPSTQARLALRISPGQGTLEASRLLEQHLRNNTPFNARVEVFDITAGEPYRAV
CCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCHHH
AGDKYLKAMRCAMEEAWGKRPALIGIGCSIPIVSMLQSEFPQMSLLVTGVEDPKTYAHSP
CCHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCEEEEEECCCCCHHHCCCC
NESLSLKVFRNAIAAQVYFLLSLGGE
CCCEEHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MHKDDLDREYSQALRDLKELIRFSTVAWPGFDRSVLFESAERIRALLVDTDFFDCVEVVD
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHH
HCGIPSVFAQKAPAPGYPTVLLYAHHDVQPAGDPDKWVTPAFSPDERDGRLYGRGAADDK
HCCCCHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCC
VAIAMHLASVRILKTLNSKIGVRVFIEGEEEILSPNFPKLLKSRRSFFDADVAIIADSGN
EEEHHHHHHHHHHHHHCCCCCEEEEEECCHHHCCCCHHHHHHHHHHHCCCCEEEEECCCC
PDEDTPAITVSLRGSIGATVRVTTLSHAAHSGLGGAVPDALMVMIRLLNSLYDDSGSVAI
CCCCCCEEEEEECCCCCCEEEEEEEHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEE
EGLKTTDPSSVLTDFELTEQALRNEVGVLPDVSLVGRGSISERTWHAPSITIIGLDVENV
EECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCEECCCEEEEEEECCCCC
DLASNTLQPSTQARLALRISPGQGTLEASRLLEQHLRNNTPFNARVEVFDITAGEPYRAV
CCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCHHH
AGDKYLKAMRCAMEEAWGKRPALIGIGCSIPIVSMLQSEFPQMSLLVTGVEDPKTYAHSP
CCHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCEEEEEECCCCCHHHCCCC
NESLSLKVFRNAIAAQVYFLLSLGGE
CCCEEHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA