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The map label for this gene is dnaG [H]

Identifier: 28572633

GI number: 28572633

Start: 522062

End: 523873

Strand: Reverse

Name: dnaG [H]

Synonym: TW484

Alternate gene names: 28572633

Gene position: 523873-522062 (Counterclockwise)

Preceding gene: 28572634

Following gene: 28572632

Centisome position: 56.58

GC content: 44.92

Gene sequence:

>1812_bases
ATGAGCAAGATCAAGCAAGAGGTTATTCACGAAATAAAAGAAAGAGCAAACATCCGAGAGATTATTGGCGAGTACACAAA
CTTGAAGCGTCAGTCTGCTGGTGCATACGCCGGGTTGTGTCCATTTCATAGCGAGCGAACACCGAGTTTTCATGTTCGTG
AAACGGGTCTATATCATTGCTTTGGCTGCGGTAAGGGTGGGGATGTTATTAGCTTTGTTCAGGAAATAGAAGGCCTAAAT
TTTGTTGATGCGGTCGAGCAGCTCGCAAGAACTCTAGGGATCACAATTGTTTATTATGAAGGTGACTCCACGCCGGATAG
TAGAAGTAATCTGCTTGCCGCCTGCAAATCTGCCGCAGAGTTCTATTCTGAACAGCTACTGAGTTCCACCCCCGCAGTCA
CTTTCCTAAAGTCTCGGGGATTTGACAGAGACCTGTGTGAGAGATATCAAATTGGTTTTTCGCCCACGAATTCTGGTGAC
CTTTATAAACACCTAAGAGATAAAGGATTTACCCTACAACAAGCAGTTGCAGCGGGATTGCTAAATCCATCTGGTATGGA
TCGCTTCAGAGCACGAATAGTATGGCCGGTGAAGGATGTTACTGGAAACATAGTAGGCTTCGGTGCAAGAAAACTTGCTG
AAGATGCTCAGGGACCAAAGTATATAAACAGTCCCGAAAGCAGCCTATATAAAAAGAATTCCCTCCTATATGGGTTTGAT
GTTGCAAAAAAGGCTATTTCAAAGAAGGATCGTGTTGTGATTGTTGAGGGTTATACAGATGTTATGTCCTGTCACCAGTC
TGGCATTGATGAAGCCGTCGCAACTTGTGGCACTTCTTGGGGTATCGGTCATATGAGGCTGATAAAGCGGATGCTTTCCG
ATTCCGGAGTGCTTATCTTTGCTTTTGACGGTGATCAAGCAGGTCAGCATGCAATTATGCGCGCATTCAAAGACAGCCCG
GAGATAGCTGCCAGATCATATGCTGCAATTTTCCCAAAAGGACTTGACCCATGTGAGCTGCGCATTAAAAATGGAGCGCA
GGCAGTTATTGACTTGGTTGAGCGGGCTATTCCGTTGTACGAATTTGTAATTAATCATCAGTTGTCTAAATGCCCACTGG
GGACATTCCCGGAGAGGCGTGCAGCACTTGCAAAGATTTTGCCTTTCTTGAAAGAAATTTCTGACAGAATCCTTCGTGAT
CATTACACAGGCCTTGTTTGCAGTCGGCTTGGAATTGACAGAGAGCAGGTCGATTTACTTTTGCCAAAACTTGAACATAT
ATCTTCTAGATTCCATCCTTCAAAAATCCCATGGCAGGAAGATTGGAAAAAAAAGGAGTTTGCTGCCCCTACCAATCCTC
CTGATGCCAGATTTGCGCTTGAGCAACAAGTTATAAAGAGTTTGTTGCAGTACCAGCACCTTCTTGAAAGGGATACCGCT
ATTGCCATGTGCGACTATTCCTTTCACAACCCAACCCTCTCTTTTATTGCCCAGATGATAGGCATGGTGATTCGGGAGCA
GAATAGGAGTATCTCTCCTGCTGAATTTTCAAAATTAATCCATTCAACAGTTCCAAGTGAATATCACGCCACTCTACAGG
CCCTTGAAGTTCTGCCCGTACCAGTGTCTCCGGATAAATTGGATTCATATTTAGAGGGCCTTGTGAGGAGTTTTGAAAAT
CAGCATCTTCTCGAAGAAAAGGCACTTCTTTTGAGGCAACTTAGCTCGGAGAAGAGCCTGGAGAAGAGCCTGGAACTACG
ACAGAGGATTGTTGAGCTTGAGAAGCAGCGCAACAGTCTTTTGCCAGACTGA

Upstream 100 bases:

>100_bases
TTACTGATCTTGATGCTCTATTACTTATGCAACGGTACTGCTCAGAATCAGATGCTGTAAGAGAATTTCTCATGAAATCA
GAGAGCGCGAGATCACAATT

Downstream 100 bases:

>100_bases
GTATCTAAAGGCGGATATTACTCTGGATGCTTTAGGTGTTCCTTTGTGATATTGCGCAATTTCGCAGCGCAATACACAAA
GGAAGATTAATTATTCGTAA

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 603; Mature: 602

Protein sequence:

>603_residues
MSKIKQEVIHEIKERANIREIIGEYTNLKRQSAGAYAGLCPFHSERTPSFHVRETGLYHCFGCGKGGDVISFVQEIEGLN
FVDAVEQLARTLGITIVYYEGDSTPDSRSNLLAACKSAAEFYSEQLLSSTPAVTFLKSRGFDRDLCERYQIGFSPTNSGD
LYKHLRDKGFTLQQAVAAGLLNPSGMDRFRARIVWPVKDVTGNIVGFGARKLAEDAQGPKYINSPESSLYKKNSLLYGFD
VAKKAISKKDRVVIVEGYTDVMSCHQSGIDEAVATCGTSWGIGHMRLIKRMLSDSGVLIFAFDGDQAGQHAIMRAFKDSP
EIAARSYAAIFPKGLDPCELRIKNGAQAVIDLVERAIPLYEFVINHQLSKCPLGTFPERRAALAKILPFLKEISDRILRD
HYTGLVCSRLGIDREQVDLLLPKLEHISSRFHPSKIPWQEDWKKKEFAAPTNPPDARFALEQQVIKSLLQYQHLLERDTA
IAMCDYSFHNPTLSFIAQMIGMVIREQNRSISPAEFSKLIHSTVPSEYHATLQALEVLPVPVSPDKLDSYLEGLVRSFEN
QHLLEEKALLLRQLSSEKSLEKSLELRQRIVELEKQRNSLLPD

Sequences:

>Translated_603_residues
MSKIKQEVIHEIKERANIREIIGEYTNLKRQSAGAYAGLCPFHSERTPSFHVRETGLYHCFGCGKGGDVISFVQEIEGLN
FVDAVEQLARTLGITIVYYEGDSTPDSRSNLLAACKSAAEFYSEQLLSSTPAVTFLKSRGFDRDLCERYQIGFSPTNSGD
LYKHLRDKGFTLQQAVAAGLLNPSGMDRFRARIVWPVKDVTGNIVGFGARKLAEDAQGPKYINSPESSLYKKNSLLYGFD
VAKKAISKKDRVVIVEGYTDVMSCHQSGIDEAVATCGTSWGIGHMRLIKRMLSDSGVLIFAFDGDQAGQHAIMRAFKDSP
EIAARSYAAIFPKGLDPCELRIKNGAQAVIDLVERAIPLYEFVINHQLSKCPLGTFPERRAALAKILPFLKEISDRILRD
HYTGLVCSRLGIDREQVDLLLPKLEHISSRFHPSKIPWQEDWKKKEFAAPTNPPDARFALEQQVIKSLLQYQHLLERDTA
IAMCDYSFHNPTLSFIAQMIGMVIREQNRSISPAEFSKLIHSTVPSEYHATLQALEVLPVPVSPDKLDSYLEGLVRSFEN
QHLLEEKALLLRQLSSEKSLEKSLELRQRIVELEKQRNSLLPD
>Mature_602_residues
SKIKQEVIHEIKERANIREIIGEYTNLKRQSAGAYAGLCPFHSERTPSFHVRETGLYHCFGCGKGGDVISFVQEIEGLNF
VDAVEQLARTLGITIVYYEGDSTPDSRSNLLAACKSAAEFYSEQLLSSTPAVTFLKSRGFDRDLCERYQIGFSPTNSGDL
YKHLRDKGFTLQQAVAAGLLNPSGMDRFRARIVWPVKDVTGNIVGFGARKLAEDAQGPKYINSPESSLYKKNSLLYGFDV
AKKAISKKDRVVIVEGYTDVMSCHQSGIDEAVATCGTSWGIGHMRLIKRMLSDSGVLIFAFDGDQAGQHAIMRAFKDSPE
IAARSYAAIFPKGLDPCELRIKNGAQAVIDLVERAIPLYEFVINHQLSKCPLGTFPERRAALAKILPFLKEISDRILRDH
YTGLVCSRLGIDREQVDLLLPKLEHISSRFHPSKIPWQEDWKKKEFAAPTNPPDARFALEQQVIKSLLQYQHLLERDTAI
AMCDYSFHNPTLSFIAQMIGMVIREQNRSISPAEFSKLIHSTVPSEYHATLQALEVLPVPVSPDKLDSYLEGLVRSFENQ
HLLEEKALLLRQLSSEKSLEKSLELRQRIVELEKQRNSLLPD

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=439, Percent_Identity=34.624145785877, Blast_Score=234, Evalue=1e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 67693; Mature: 67562

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIKQEVIHEIKERANIREIIGEYTNLKRQSAGAYAGLCPFHSERTPSFHVRETGLYHC
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCEEE
FGCGKGGDVISFVQEIEGLNFVDAVEQLARTLGITIVYYEGDSTPDSRSNLLAACKSAAE
EECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHH
FYSEQLLSSTPAVTFLKSRGFDRDLCERYQIGFSPTNSGDLYKHLRDKGFTLQQAVAAGL
HHHHHHHHCCCHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHC
LNPSGMDRFRARIVWPVKDVTGNIVGFGARKLAEDAQGPKYINSPESSLYKKNSLLYGFD
CCCCCHHHHHHEEEEEHHHHCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCEEHHH
VAKKAISKKDRVVIVEGYTDVMSCHQSGIDEAVATCGTSWGIGHMRLIKRMLSDSGVLIF
HHHHHHCCCCCEEEEECCHHHHHHHHHCHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEE
AFDGDQAGQHAIMRAFKDSPEIAARSYAAIFPKGLDPCELRIKNGAQAVIDLVERAIPLY
EECCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHEECCCHHHHHHHHHHHHHHH
EFVINHQLSKCPLGTFPERRAALAKILPFLKEISDRILRDHYTGLVCSRLGIDREQVDLL
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
LPKLEHISSRFHPSKIPWQEDWKKKEFAAPTNPPDARFALEQQVIKSLLQYQHLLERDTA
HHHHHHHHHCCCCCCCCCHHHHCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCE
IAMCDYSFHNPTLSFIAQMIGMVIREQNRSISPAEFSKLIHSTVPSEYHATLQALEVLPV
EEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCC
PVSPDKLDSYLEGLVRSFENQHLLEEKALLLRQLSSEKSLEKSLELRQRIVELEKQRNSL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LPD
CCC
>Mature Secondary Structure 
SKIKQEVIHEIKERANIREIIGEYTNLKRQSAGAYAGLCPFHSERTPSFHVRETGLYHC
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCEEE
FGCGKGGDVISFVQEIEGLNFVDAVEQLARTLGITIVYYEGDSTPDSRSNLLAACKSAAE
EECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHH
FYSEQLLSSTPAVTFLKSRGFDRDLCERYQIGFSPTNSGDLYKHLRDKGFTLQQAVAAGL
HHHHHHHHCCCHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHC
LNPSGMDRFRARIVWPVKDVTGNIVGFGARKLAEDAQGPKYINSPESSLYKKNSLLYGFD
CCCCCHHHHHHEEEEEHHHHCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCEEHHH
VAKKAISKKDRVVIVEGYTDVMSCHQSGIDEAVATCGTSWGIGHMRLIKRMLSDSGVLIF
HHHHHHCCCCCEEEEECCHHHHHHHHHCHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEE
AFDGDQAGQHAIMRAFKDSPEIAARSYAAIFPKGLDPCELRIKNGAQAVIDLVERAIPLY
EECCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHEECCCHHHHHHHHHHHHHHH
EFVINHQLSKCPLGTFPERRAALAKILPFLKEISDRILRDHYTGLVCSRLGIDREQVDLL
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
LPKLEHISSRFHPSKIPWQEDWKKKEFAAPTNPPDARFALEQQVIKSLLQYQHLLERDTA
HHHHHHHHHCCCCCCCCCHHHHCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCE
IAMCDYSFHNPTLSFIAQMIGMVIREQNRSISPAEFSKLIHSTVPSEYHATLQALEVLPV
EEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCC
PVSPDKLDSYLEGLVRSFENQHLLEEKALLLRQLSSEKSLEKSLELRQRIVELEKQRNSL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LPD
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]