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The map label for this gene is ligA

Identifier: 28572508

GI number: 28572508

Start: 386982

End: 388868

Strand: Direct

Name: ligA

Synonym: TW354

Alternate gene names: 28572508

Gene position: 386982-388868 (Clockwise)

Preceding gene: 28572507

Following gene: 28572509

Centisome position: 41.79

GC content: 43.93

Gene sequence:

>1887_bases
ATGCCTCATCCTGACGGGGATCTTGATAGACGCATTGAACTTCTAACGGCCCAGATAATTGCGGCACGGAAGGCGTATTA
TCAAGAGAACACCTCTCTCATGAGTGATGTTGAGTATGATGCCCTGGAGCATGAGCTAAAAGATGCCGAGCATGCAAAAG
GCTTTTCTGACCGCAATTCACCTAGCTTAACAGTTGGGATTGCCGCACAGCTAAACCTATTTGAGCCCGTGAAACACATC
GAGCCAATGTTGAGTTTAGATAATGTTTTTTCGCTCGATCAACTCCATTCATGGTACGAAAAGACAAAGAAGATTTGCCC
TGAGGGTGATCAATGCACATTTGTTTGTGAGCTGAAAATTGATGGCGTTGGTGTGAGTTTGCGTTATGCAAACGGGTATT
TGATTTCAGCAGCTACCAGAGGTGATGGTGCTATCGGCGAGGATATAACCCAAAACATGTTATATGTTCCGTCCATCCCT
CCCAGGATCGCTTTACCGGGAATATTTGAAATTCGCGGCGAAGCTTTTATAAAGCGCGATGAGTTTGACAGAATAAACCA
ATTGTCATTGGAGCGTAGCAAGCAGTTTGCGAATCCAAGAAATTTTGTAAGCGGATGTATAAGAACAAAAACACCAAATA
TGCGATACTTGGAGTCAATTTCCTTCTATGCTCATGGATTTACGCAAGTTTATGGATATACCTCAGGAGGCATGAATCTT
CACTCTGATATAACTGCTTCTGGCGGCGTTAAGACTGAAATAGAACATGGTATGTTTTCTGCATATTCCAGGCTGTCTGA
ATGTAAAATCCCAGTAAACTCGTACAATCGCCTGTGCACTAATTTCTCTGAAATCGAGTCTTATATTGAAAACATTCGAT
TGAATCGGCAGTGCGTTCCGTACGCAATCGACGGTATCGTTGTAAAGATCGATAGCCTCCAAAAACAGGCACTTTTAGGC
AGTACAACAAAAGCACCACGGTGGGCTGTTGCGTATAAATTTCCTTCTGAATCTACTGTAACGCGTCTTCTCGATATAGA
AGTGAGTGTTGGAAGAACGGGACGTGTAACGCCTTATGCCGTTCTTCAACCAATTCAACTCGATGGGTCTGAAGTATCGC
GTGCAACTTTGCATAATCAGAAGGTAATAGGTGATAAGGATTTACTAATCGGTGATTACGTGAGGATCAGAAAAGCTGGC
GATATTGTTCCCGAGGTGCTTTGCGCCCTGCCAGAGAAACGCGATGGCAGTGAGGTTTTATTCAAGATGCCATCGCTCTG
TCCGTCGTGCGGTGCAGAACTTATGCCAAGTAAGTTAGGGGATATCGACCTTAGGTGTCCAAATATGCAGAGCTGTCTTG
TACAGCTCGCGGGACGTCTTGAGTATATAGGTTCACGCGGTGTGCTGGATATTGCTTACCTTGCAGAAGAGAATGCATAC
GCCCTGAGTCACCTTAGAAAGTTCGGAAAGTCTGCGGAGGTTCAACTCTTTAAAATTACAATTGATGATCTGGTTGCTCT
TGAATTTATGTATAAAGGAAACATGCGATCGCCTTTTCGTAAAAAGGGTGATAGTTTCCCAGGATTTGAGGAACCCACAA
AATCTGCACAGGATATGGTAGATAGTATTGAAAGAGCAAAGCGGTCCCCACTCTGGAAATTTCTGCTCGCGCTAAATATA
CGTCATATCGGTCCCGCATCCGCAAAGGCTCTGGCAGATCATTTTGGCTCTATTGAATCCATAATAAATGCCAAAATAGA
TGAATTGCTGAAGGTTAGATCCTTAGGAGAAACAATAGCTATTTCGGTGCATGACTGGTTTAGAGACCCATGGCGGGTGG
AATTGGTTAATACTTGGCGTTCCGATGGAGCACTTTTTGGGGATTAA

Upstream 100 bases:

>100_bases
CCTGATGAACCCGTTTTTGCAGTTGCAAAGGGGCAAAGTGCGGCTATTTATCGTGGAACAAGAGTTCTTGGACAGCTTAT
GATTGACAACACGAAGAAAT

Downstream 100 bases:

>100_bases
AGATTTCAGTTCCAGCATTTGCGTTTTTATTTCGTTTGGTGAAACTTTGATGTTTTACCATTATGCCTTTGTAGAATGGA
ATTCCATGCAGGCCCGGCGG

Product: DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 628; Mature: 627

Protein sequence:

>628_residues
MPHPDGDLDRRIELLTAQIIAARKAYYQENTSLMSDVEYDALEHELKDAEHAKGFSDRNSPSLTVGIAAQLNLFEPVKHI
EPMLSLDNVFSLDQLHSWYEKTKKICPEGDQCTFVCELKIDGVGVSLRYANGYLISAATRGDGAIGEDITQNMLYVPSIP
PRIALPGIFEIRGEAFIKRDEFDRINQLSLERSKQFANPRNFVSGCIRTKTPNMRYLESISFYAHGFTQVYGYTSGGMNL
HSDITASGGVKTEIEHGMFSAYSRLSECKIPVNSYNRLCTNFSEIESYIENIRLNRQCVPYAIDGIVVKIDSLQKQALLG
STTKAPRWAVAYKFPSESTVTRLLDIEVSVGRTGRVTPYAVLQPIQLDGSEVSRATLHNQKVIGDKDLLIGDYVRIRKAG
DIVPEVLCALPEKRDGSEVLFKMPSLCPSCGAELMPSKLGDIDLRCPNMQSCLVQLAGRLEYIGSRGVLDIAYLAEENAY
ALSHLRKFGKSAEVQLFKITIDDLVALEFMYKGNMRSPFRKKGDSFPGFEEPTKSAQDMVDSIERAKRSPLWKFLLALNI
RHIGPASAKALADHFGSIESIINAKIDELLKVRSLGETIAISVHDWFRDPWRVELVNTWRSDGALFGD

Sequences:

>Translated_628_residues
MPHPDGDLDRRIELLTAQIIAARKAYYQENTSLMSDVEYDALEHELKDAEHAKGFSDRNSPSLTVGIAAQLNLFEPVKHI
EPMLSLDNVFSLDQLHSWYEKTKKICPEGDQCTFVCELKIDGVGVSLRYANGYLISAATRGDGAIGEDITQNMLYVPSIP
PRIALPGIFEIRGEAFIKRDEFDRINQLSLERSKQFANPRNFVSGCIRTKTPNMRYLESISFYAHGFTQVYGYTSGGMNL
HSDITASGGVKTEIEHGMFSAYSRLSECKIPVNSYNRLCTNFSEIESYIENIRLNRQCVPYAIDGIVVKIDSLQKQALLG
STTKAPRWAVAYKFPSESTVTRLLDIEVSVGRTGRVTPYAVLQPIQLDGSEVSRATLHNQKVIGDKDLLIGDYVRIRKAG
DIVPEVLCALPEKRDGSEVLFKMPSLCPSCGAELMPSKLGDIDLRCPNMQSCLVQLAGRLEYIGSRGVLDIAYLAEENAY
ALSHLRKFGKSAEVQLFKITIDDLVALEFMYKGNMRSPFRKKGDSFPGFEEPTKSAQDMVDSIERAKRSPLWKFLLALNI
RHIGPASAKALADHFGSIESIINAKIDELLKVRSLGETIAISVHDWFRDPWRVELVNTWRSDGALFGD
>Mature_627_residues
PHPDGDLDRRIELLTAQIIAARKAYYQENTSLMSDVEYDALEHELKDAEHAKGFSDRNSPSLTVGIAAQLNLFEPVKHIE
PMLSLDNVFSLDQLHSWYEKTKKICPEGDQCTFVCELKIDGVGVSLRYANGYLISAATRGDGAIGEDITQNMLYVPSIPP
RIALPGIFEIRGEAFIKRDEFDRINQLSLERSKQFANPRNFVSGCIRTKTPNMRYLESISFYAHGFTQVYGYTSGGMNLH
SDITASGGVKTEIEHGMFSAYSRLSECKIPVNSYNRLCTNFSEIESYIENIRLNRQCVPYAIDGIVVKIDSLQKQALLGS
TTKAPRWAVAYKFPSESTVTRLLDIEVSVGRTGRVTPYAVLQPIQLDGSEVSRATLHNQKVIGDKDLLIGDYVRIRKAGD
IVPEVLCALPEKRDGSEVLFKMPSLCPSCGAELMPSKLGDIDLRCPNMQSCLVQLAGRLEYIGSRGVLDIAYLAEENAYA
LSHLRKFGKSAEVQLFKITIDDLVALEFMYKGNMRSPFRKKGDSFPGFEEPTKSAQDMVDSIERAKRSPLWKFLLALNIR
HIGPASAKALADHFGSIESIINAKIDELLKVRSLGETIAISVHDWFRDPWRVELVNTWRSDGALFGD

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD-dependent DNA ligase family. LigA subfamily

Homologues:

Organism=Escherichia coli, GI1788750, Length=606, Percent_Identity=33.993399339934, Blast_Score=328, Evalue=6e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_TROW8 (Q83HX4)

Other databases:

- EMBL:   BX251411
- RefSeq:   NP_789288.1
- HSSP:   Q9ZHI0
- ProteinModelPortal:   Q83HX4
- SMR:   Q83HX4
- STRING:   Q83HX4
- GeneID:   1064641
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW354
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- PhylomeDB:   Q83HX4
- ProtClustDB:   CLSK229677
- BioCyc:   TWHI218496:TW0333-MONOMER
- HAMAP:   MF_01588
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 70187; Mature: 70056

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 119-119 BINDING 117-117 BINDING 140-140 BINDING 174-174 BINDING 309-309 BINDING 333-333

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHPDGDLDRRIELLTAQIIAARKAYYQENTSLMSDVEYDALEHELKDAEHAKGFSDRNS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PSLTVGIAAQLNLFEPVKHIEPMLSLDNVFSLDQLHSWYEKTKKICPEGDQCTFVCELKI
CCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEE
DGVGVSLRYANGYLISAATRGDGAIGEDITQNMLYVPSIPPRIALPGIFEIRGEAFIKRD
CCEEEEEEEECCEEEEEECCCCCCCCHHHHHCEEECCCCCCCCCCCCCEEECCCCEEECC
EFDRINQLSLERSKQFANPRNFVSGCIRTKTPNMRYLESISFYAHGFTQVYGYTSGGMNL
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHEEEECCCCCEE
HSDITASGGVKTEIEHGMFSAYSRLSECKIPVNSYNRLCTNFSEIESYIENIRLNRQCVP
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCC
YAIDGIVVKIDSLQKQALLGSTTKAPRWAVAYKFPSESTVTRLLDIEVSVGRTGRVTPYA
EEECCEEEEECHHHHHHHHCCCCCCCCEEEEEECCCCHHEEEEEEEEEECCCCCCCCHHH
VLQPIQLDGSEVSRATLHNQKVIGDKDLLIGDYVRIRKAGDIVPEVLCALPEKRDGSEVL
HEEEEECCCCHHHHHHHCCCCCCCCCCCEEHHHHHHHHHCCHHHHHHHHCCCCCCCHHHH
FKMPSLCPSCGAELMPSKLGDIDLRCPNMQSCLVQLAGRLEYIGSRGVLDIAYLAEENAY
HHHHCCCHHCCHHHCCCCCCCCEECCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHH
ALSHLRKFGKSAEVQLFKITIDDLVALEFMYKGNMRSPFRKKGDSFPGFEEPTKSAQDMV
HHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCHHHHHHHHH
DSIERAKRSPLWKFLLALNIRHIGPASAKALADHFGSIESIINAKIDELLKVRSLGETIA
HHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
ISVHDWFRDPWRVELVNTWRSDGALFGD
EEEHHHHCCCEEEEEEEHHCCCCCCCCC
>Mature Secondary Structure 
PHPDGDLDRRIELLTAQIIAARKAYYQENTSLMSDVEYDALEHELKDAEHAKGFSDRNS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PSLTVGIAAQLNLFEPVKHIEPMLSLDNVFSLDQLHSWYEKTKKICPEGDQCTFVCELKI
CCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEE
DGVGVSLRYANGYLISAATRGDGAIGEDITQNMLYVPSIPPRIALPGIFEIRGEAFIKRD
CCEEEEEEEECCEEEEEECCCCCCCCHHHHHCEEECCCCCCCCCCCCCEEECCCCEEECC
EFDRINQLSLERSKQFANPRNFVSGCIRTKTPNMRYLESISFYAHGFTQVYGYTSGGMNL
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHEEEECCCCCEE
HSDITASGGVKTEIEHGMFSAYSRLSECKIPVNSYNRLCTNFSEIESYIENIRLNRQCVP
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCC
YAIDGIVVKIDSLQKQALLGSTTKAPRWAVAYKFPSESTVTRLLDIEVSVGRTGRVTPYA
EEECCEEEEECHHHHHHHHCCCCCCCCEEEEEECCCCHHEEEEEEEEEECCCCCCCCHHH
VLQPIQLDGSEVSRATLHNQKVIGDKDLLIGDYVRIRKAGDIVPEVLCALPEKRDGSEVL
HEEEEECCCCHHHHHHHCCCCCCCCCCCEEHHHHHHHHHCCHHHHHHHHCCCCCCCHHHH
FKMPSLCPSCGAELMPSKLGDIDLRCPNMQSCLVQLAGRLEYIGSRGVLDIAYLAEENAY
HHHHCCCHHCCHHHCCCCCCCCEECCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHH
ALSHLRKFGKSAEVQLFKITIDDLVALEFMYKGNMRSPFRKKGDSFPGFEEPTKSAQDMV
HHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCHHHHHHHHH
DSIERAKRSPLWKFLLALNIRHIGPASAKALADHFGSIESIINAKIDELLKVRSLGETIA
HHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
ISVHDWFRDPWRVELVNTWRSDGALFGD
EEEHHHHCCCEEEEEEEHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12606174