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The map label for this gene is obgE

Identifier: 28572450

GI number: 28572450

Start: 332125

End: 333462

Strand: Direct

Name: obgE

Synonym: TW294

Alternate gene names: 28572450

Gene position: 332125-333462 (Clockwise)

Preceding gene: 28572449

Following gene: 28572451

Centisome position: 35.87

GC content: 47.38

Gene sequence:

>1338_bases
GTGTCGGGGATCACTCTCCCTGAGTTTGTCGATTGTGTAACTGTAGAATTTTCTGCTGGGCGCGGTGGTGATGGCTGCGC
CTCTGTGAGGCGCGAAAAGTATAAGCCTCTTGCTGGCCCTGACGGTGGGAGTGGAGGTCACGGGGGTAGCATTTTTTTAA
AGGCTGATACTTCTGAACGTACTCTTATTTCCTTTCGCCGCAAGGGCCATTACTCTGCCAGTAATGGGGCACATGGCCTG
AGTAGACTGCGTAATGGTGCTCGCGGTAAGGACTTAGAAGTCTCTGTCCCCTGCGGCACATCTGTTTATGATGAAGGCGG
TAGGCAAATAGCTGATCTTGTATCGCCTGGCAGTTGCCTGCAGGTCGTTCGTGGGGGCACTGGTGGCCTTGGAAATGCCG
CTCTTGCAGGTTATAGGCGCAAAACCCCAAGGTTTGCCCTGTTGGGCTTGCCCGGCCAGAAGCGCAAGCTCAGGCTTGAG
GTCAAATCTATTGCAGATGTTGCACTTGTTGGTTTTCCTTCGGTTGGAAAATCAAGCATAATCAGTGCAATCTCATCTGC
AAAGCCAAAAATTGCGGATTATCCGTTTACAACCCTCCATCCCAATCTCGGTGTTGTGCAATCTGGCCCGTACAGATACA
CGGTTGCTGATGTCCCAGGCCTCGTTGAAGGCGCCAGTAAGGGTATTGGTCTAGGCTTAAATTTTCTCAGACATATTGAG
CGGTGTAGCGTTGTGGTCCATGTAATAGATTGCGCAAATACACAGCAAGATCCCATATCAGGTTTTAATTTAATAGAAAA
AGAGTTGTCAGAATACAAAGTTGCAGAGAACGCGATTCCTCTGAACAAAAGGCCTAAAGTTATTGTTCTCAATAAAATTG
ATGTCTTGCAGACTAAGGAGGAGCAGGATACTTTGCTTTATCTGCAATCAGTTTTCAAGAAATTGGTCACAGATGTGTAT
GCAATTTCCGCCGTAACCCGTTCAGGGTTGCGACAGTTTACTCTGAGACTTGGCGAAATATGTCAGGAATACCCCTCACA
AGTGCAACCGACATCACAGACCATATTGATCCCTGCAAAAAATACCCCTGAATTTTCCTTAGATAGAACGGATGGAGTGT
ATAGAGTGACCGGCAAGAAACCGGAAAAGTGGATTCTGCAAACTGATTTTTCAAGTGATGAAGCAATTAGTTATCTTGCA
GAAAGACTAGATCGCCTGGGAATTGAAGATGCGTTGGTCCGTGCCGGTGCAACTTGTGGTGATGAGGTTGAAATCGGCGG
CGTAATCTTTACATGGGATCCGAGTGTTGCCAACACATCATTTTCTCTCTCTGTGTGA

Upstream 100 bases:

>100_bases
ATGTGCGCCGCGGGGATGATGATACCCTTTTTGCTTGCGCTTCTGGCCGGGTCAGGTTCGGCAGATCTGGAAAGCGCAGG
GCTGTTAGCGTAGACACGTA

Downstream 100 bases:

>100_bases
TTCCCATATATTAACATGCACCCACCTAAGTTATCTTTAGATTGTTTGGTATGTAGTATTGAATCGTGCTAACCGTGTCG
TCTATGTGGGTGTTTAAACT

Product: GTPase ObgE

Products: NA

Alternate protein names: GTP-binding protein obg

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MSGITLPEFVDCVTVEFSAGRGGDGCASVRREKYKPLAGPDGGSGGHGGSIFLKADTSERTLISFRRKGHYSASNGAHGL
SRLRNGARGKDLEVSVPCGTSVYDEGGRQIADLVSPGSCLQVVRGGTGGLGNAALAGYRRKTPRFALLGLPGQKRKLRLE
VKSIADVALVGFPSVGKSSIISAISSAKPKIADYPFTTLHPNLGVVQSGPYRYTVADVPGLVEGASKGIGLGLNFLRHIE
RCSVVVHVIDCANTQQDPISGFNLIEKELSEYKVAENAIPLNKRPKVIVLNKIDVLQTKEEQDTLLYLQSVFKKLVTDVY
AISAVTRSGLRQFTLRLGEICQEYPSQVQPTSQTILIPAKNTPEFSLDRTDGVYRVTGKKPEKWILQTDFSSDEAISYLA
ERLDRLGIEDALVRAGATCGDEVEIGGVIFTWDPSVANTSFSLSV

Sequences:

>Translated_445_residues
MSGITLPEFVDCVTVEFSAGRGGDGCASVRREKYKPLAGPDGGSGGHGGSIFLKADTSERTLISFRRKGHYSASNGAHGL
SRLRNGARGKDLEVSVPCGTSVYDEGGRQIADLVSPGSCLQVVRGGTGGLGNAALAGYRRKTPRFALLGLPGQKRKLRLE
VKSIADVALVGFPSVGKSSIISAISSAKPKIADYPFTTLHPNLGVVQSGPYRYTVADVPGLVEGASKGIGLGLNFLRHIE
RCSVVVHVIDCANTQQDPISGFNLIEKELSEYKVAENAIPLNKRPKVIVLNKIDVLQTKEEQDTLLYLQSVFKKLVTDVY
AISAVTRSGLRQFTLRLGEICQEYPSQVQPTSQTILIPAKNTPEFSLDRTDGVYRVTGKKPEKWILQTDFSSDEAISYLA
ERLDRLGIEDALVRAGATCGDEVEIGGVIFTWDPSVANTSFSLSV
>Mature_444_residues
SGITLPEFVDCVTVEFSAGRGGDGCASVRREKYKPLAGPDGGSGGHGGSIFLKADTSERTLISFRRKGHYSASNGAHGLS
RLRNGARGKDLEVSVPCGTSVYDEGGRQIADLVSPGSCLQVVRGGTGGLGNAALAGYRRKTPRFALLGLPGQKRKLRLEV
KSIADVALVGFPSVGKSSIISAISSAKPKIADYPFTTLHPNLGVVQSGPYRYTVADVPGLVEGASKGIGLGLNFLRHIER
CSVVVHVIDCANTQQDPISGFNLIEKELSEYKVAENAIPLNKRPKVIVLNKIDVLQTKEEQDTLLYLQSVFKKLVTDVYA
ISAVTRSGLRQFTLRLGEICQEYPSQVQPTSQTILIPAKNTPEFSLDRTDGVYRVTGKKPEKWILQTDFSSDEAISYLAE
RLDRLGIEDALVRAGATCGDEVEIGGVIFTWDPSVANTSFSLSV

Specific function: An essential GTPase which binds GTP, GDP and possibly (p)ppGpp with moderate affinity, with high nucleotide exchange rates and a fairly low GTP hydrolysis rate. It may play a role in control of the cell cycle, stress response, ribosome biogenesis and in t

COG id: COG0536

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI24308117, Length=337, Percent_Identity=39.1691394658754, Blast_Score=166, Evalue=5e-41,
Organism=Homo sapiens, GI111955139, Length=286, Percent_Identity=35.3146853146853, Blast_Score=152, Evalue=5e-37,
Organism=Homo sapiens, GI111955063, Length=244, Percent_Identity=31.9672131147541, Blast_Score=102, Evalue=8e-22,
Organism=Homo sapiens, GI4758796, Length=118, Percent_Identity=33.0508474576271, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI4557537, Length=105, Percent_Identity=36.1904761904762, Blast_Score=66, Evalue=6e-11,
Organism=Escherichia coli, GI1789574, Length=341, Percent_Identity=41.9354838709677, Blast_Score=229, Evalue=4e-61,
Organism=Escherichia coli, GI1787454, Length=100, Percent_Identity=37, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17508313, Length=335, Percent_Identity=38.2089552238806, Blast_Score=149, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI17552324, Length=316, Percent_Identity=30.0632911392405, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71981008, Length=131, Percent_Identity=34.3511450381679, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17555344, Length=121, Percent_Identity=33.8842975206612, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6321962, Length=189, Percent_Identity=39.6825396825397, Blast_Score=121, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6319281, Length=129, Percent_Identity=33.3333333333333, Blast_Score=70, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6321612, Length=129, Percent_Identity=36.4341085271318, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24585318, Length=299, Percent_Identity=34.7826086956522, Blast_Score=143, Evalue=3e-34,
Organism=Drosophila melanogaster, GI20129375, Length=346, Percent_Identity=34.393063583815, Blast_Score=141, Evalue=7e-34,
Organism=Drosophila melanogaster, GI17981711, Length=130, Percent_Identity=33.8461538461538, Blast_Score=72, Evalue=8e-13,
Organism=Drosophila melanogaster, GI21356473, Length=120, Percent_Identity=33.3333333333333, Blast_Score=69, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24640873, Length=172, Percent_Identity=27.906976744186, Blast_Score=66, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24640877, Length=172, Percent_Identity=27.906976744186, Blast_Score=66, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24640875, Length=172, Percent_Identity=27.906976744186, Blast_Score=66, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): OBG_TROW8 (Q83NP1)

Other databases:

- EMBL:   BX251411
- RefSeq:   NP_789230.1
- HSSP:   P20964
- ProteinModelPortal:   Q83NP1
- SMR:   Q83NP1
- STRING:   Q83NP1
- GeneID:   1064583
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW294
- eggNOG:   COG0536
- HOGENOM:   HBG716038
- OMA:   HFTTLVP
- ProtClustDB:   PRK12297
- BioCyc:   TWHI218496:TW0275-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01454
- InterPro:   IPR014100
- InterPro:   IPR015349
- InterPro:   IPR006074
- InterPro:   IPR006073
- InterPro:   IPR006169
- InterPro:   IPR002917
- InterPro:   IPR005225
- Gene3D:   G3DSA:2.70.210.12
- PANTHER:   PTHR11702:SF3
- PIRSF:   PIRSF002401
- PRINTS:   PR00326
- TIGRFAMs:   TIGR02729
- TIGRFAMs:   TIGR03595
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF09269 DUF1967; PF01018 GTP1_OBG; PF01926 MMR_HSR1; SSF102741 GTP-bd_prot_GTP1/OBG_C; SSF82051 GTP1_OBG_sub

EC number: NA

Molecular weight: Translated: 47790; Mature: 47659

Theoretical pI: Translated: 8.68; Mature: 8.68

Prosite motif: PS00905 GTP1_OBG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGITLPEFVDCVTVEFSAGRGGDGCASVRREKYKPLAGPDGGSGGHGGSIFLKADTSER
CCCCCCHHHHCEEEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCH
TLISFRRKGHYSASNGAHGLSRLRNGARGKDLEVSVPCGTSVYDEGGRQIADLVSPGSCL
HHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCEEEECCCCCCHHHCCCHHHHHHCCCHHHH
QVVRGGTGGLGNAALAGYRRKTPRFALLGLPGQKRKLRLEVKSIADVALVGFPSVGKSSI
HHHCCCCCCCCCHHHHHHHCCCCCEEEECCCCCCCEEEEEHHHHHCEEEECCCCCCHHHH
ISAISSAKPKIADYPFTTLHPNLGVVQSGPYRYTVADVPGLVEGASKGIGLGLNFLRHIE
HHHHHHCCCCCCCCCCEEECCCCCEEECCCEEEEECCCCCHHHHCCCCCCHHHHHHHHHH
RCSVVVHVIDCANTQQDPISGFNLIEKELSEYKVAENAIPLNKRPKVIVLNKIDVLQTKE
HHHHEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCHHHCCCC
EQDTLLYLQSVFKKLVTDVYAISAVTRSGLRQFTLRLGEICQEYPSQVQPTSQTILIPAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECC
NTPEFSLDRTDGVYRVTGKKPEKWILQTDFSSDEAISYLAERLDRLGIEDALVRAGATCG
CCCCCEEECCCCEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCC
DEVEIGGVIFTWDPSVANTSFSLSV
CCEEECEEEEEECCCCCCCEEEEEC
>Mature Secondary Structure 
SGITLPEFVDCVTVEFSAGRGGDGCASVRREKYKPLAGPDGGSGGHGGSIFLKADTSER
CCCCCHHHHCEEEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCH
TLISFRRKGHYSASNGAHGLSRLRNGARGKDLEVSVPCGTSVYDEGGRQIADLVSPGSCL
HHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCEEEECCCCCCHHHCCCHHHHHHCCCHHHH
QVVRGGTGGLGNAALAGYRRKTPRFALLGLPGQKRKLRLEVKSIADVALVGFPSVGKSSI
HHHCCCCCCCCCHHHHHHHCCCCCEEEECCCCCCCEEEEEHHHHHCEEEECCCCCCHHHH
ISAISSAKPKIADYPFTTLHPNLGVVQSGPYRYTVADVPGLVEGASKGIGLGLNFLRHIE
HHHHHHCCCCCCCCCCEEECCCCCEEECCCEEEEECCCCCHHHHCCCCCCHHHHHHHHHH
RCSVVVHVIDCANTQQDPISGFNLIEKELSEYKVAENAIPLNKRPKVIVLNKIDVLQTKE
HHHHEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCHHHCCCC
EQDTLLYLQSVFKKLVTDVYAISAVTRSGLRQFTLRLGEICQEYPSQVQPTSQTILIPAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECC
NTPEFSLDRTDGVYRVTGKKPEKWILQTDFSSDEAISYLAERLDRLGIEDALVRAGATCG
CCCCCEEECCCCEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCC
DEVEIGGVIFTWDPSVANTSFSLSV
CCEEECEEEEEECCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12606174