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The map label for this gene is tig [H]

Identifier: 28572437

GI number: 28572437

Start: 316355

End: 317668

Strand: Direct

Name: tig [H]

Synonym: TW281

Alternate gene names: 28572437

Gene position: 316355-317668 (Clockwise)

Preceding gene: 28572436

Following gene: 28572438

Centisome position: 34.17

GC content: 45.21

Gene sequence:

>1314_bases
ATGGGCAGAACTTTGAAGAGTATCGTACAAAGGGTCAGTGAGACAGAGCTGAAGCTGCAGGTAACTATTCCCTCCGAAAG
CTTAAAGCCCCATGTCGACAGGGCCTATAGGGAAGTTGCAAAAACTGTCAGTGTTCCGGGTTTCCGTCGTGGAAAAGTTC
CAGTCAGGATTATTGACCAAAGGATTGGAAAGCATGTAGTGCTCGATAGGGCTATCAATGACTGCATAGGCGATTTTTTC
CAATCCGCAGTTGATGAGCAGGGCCTAACCCCCGTTGCAGCACCAAAAGCGCATATCGCTTCTCTTCCGACAGAGCCCGA
CGCTTCGCTTGAAGTTGTGTTTGATGTGCATGTTTATCCGGATGTAGATTTCCCGGCTTTTGAGAATAAGCAGATAGAAG
TTGATGCAATAGATGTCACAGATAGTATGGTTGATTCTGAAATAGTTGGTATTCGGGAAAAGCTGGCAACTTTTACTGCT
TTAGATAGGCCAGCTATTGATGGCGATTTTCTGATTCTTGACTTACAGATTCTTGATGGCGATAAAGTCTTAAGCGAATC
GAATGCCGTGCCGTACGAGCTTGGTTCCAATACTCTGTTTGACGGCCTTGATTCTGCCTTGACCTCTGCCCGTGCGGGTT
ACACCACAACAATCGATCTAACCCCTCCCGTAGAAGATCCGGCAGGCGAAAACCCTCAGCAAGATCAGTTTGCTAAAAGC
GGGGATACAAGACGTGTAAAACTGAAAGTTTCCTCTGTCAATAAACGGGACTTGCCTGAGGAAAATGATGAACTTGCACG
TCTTGTCGGTGGGTTTGCAAACCTCGAGGAGTTAAGGAAGGATTCTGCAAAGAATATTGAGAGGTCAATGCTTTTTCAAA
GAAGTCTTCAGGCCAGGGATTTACTGCTCGAGCAATTGCTGTCGGAAATTACCTTTCCTCTTCCAGGACCAATAGCTGAT
GTATTAGACGAGATTAAGGATGAAGAGGAAAAGCAAAACAGAACAAAGCAAGCTCGCAGGGATATATTGCTTGATTTACT
TGCAAAGACTTTTTCTGTAACCCTCAAAGAGGATGAATTGGAGCGATATTTGTCAAGTGCCGCAGCGCAGAGAAAGATGA
GTGTTGCCACACTTGTCTCTGTGATAAAAAGCGCGGGGCAGGATAAGTATGTTATTTCTGATATTGTCCGAAACAAGGCA
CTTTCAATTGCATTGAGCAGAGTTAAAGTGATTGACTCGGCAGGAAATCCGTTGGATATTTCTGAGTTTACGATTAAGAC
TGATGGGGATCCGAAGGTCAGGAGGTCCGAATGA

Upstream 100 bases:

>100_bases
CGCCCGCTTTGGGAGCGGGAGGTCGCAGGTTCAAATCCTGTCGCCCCGACTTTATGCTGAAACATCCCTCCCCTGTTCTA
TAATTTCCGGAGAAGCAAAA

Downstream 100 bases:

>100_bases
ACAGAAGGCAAGGATTGCGCTTTGTGAGGTCTGCCTGTTTCCGTCTGTTGTGCGCATTATCCCTCGGTATTGTCATGTAT
GGAGCGTTTGCTGTTCTATC

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF [H]

Number of amino acids: Translated: 437; Mature: 436

Protein sequence:

>437_residues
MGRTLKSIVQRVSETELKLQVTIPSESLKPHVDRAYREVAKTVSVPGFRRGKVPVRIIDQRIGKHVVLDRAINDCIGDFF
QSAVDEQGLTPVAAPKAHIASLPTEPDASLEVVFDVHVYPDVDFPAFENKQIEVDAIDVTDSMVDSEIVGIREKLATFTA
LDRPAIDGDFLILDLQILDGDKVLSESNAVPYELGSNTLFDGLDSALTSARAGYTTTIDLTPPVEDPAGENPQQDQFAKS
GDTRRVKLKVSSVNKRDLPEENDELARLVGGFANLEELRKDSAKNIERSMLFQRSLQARDLLLEQLLSEITFPLPGPIAD
VLDEIKDEEEKQNRTKQARRDILLDLLAKTFSVTLKEDELERYLSSAAAQRKMSVATLVSVIKSAGQDKYVISDIVRNKA
LSIALSRVKVIDSAGNPLDISEFTIKTDGDPKVRRSE

Sequences:

>Translated_437_residues
MGRTLKSIVQRVSETELKLQVTIPSESLKPHVDRAYREVAKTVSVPGFRRGKVPVRIIDQRIGKHVVLDRAINDCIGDFF
QSAVDEQGLTPVAAPKAHIASLPTEPDASLEVVFDVHVYPDVDFPAFENKQIEVDAIDVTDSMVDSEIVGIREKLATFTA
LDRPAIDGDFLILDLQILDGDKVLSESNAVPYELGSNTLFDGLDSALTSARAGYTTTIDLTPPVEDPAGENPQQDQFAKS
GDTRRVKLKVSSVNKRDLPEENDELARLVGGFANLEELRKDSAKNIERSMLFQRSLQARDLLLEQLLSEITFPLPGPIAD
VLDEIKDEEEKQNRTKQARRDILLDLLAKTFSVTLKEDELERYLSSAAAQRKMSVATLVSVIKSAGQDKYVISDIVRNKA
LSIALSRVKVIDSAGNPLDISEFTIKTDGDPKVRRSE
>Mature_436_residues
GRTLKSIVQRVSETELKLQVTIPSESLKPHVDRAYREVAKTVSVPGFRRGKVPVRIIDQRIGKHVVLDRAINDCIGDFFQ
SAVDEQGLTPVAAPKAHIASLPTEPDASLEVVFDVHVYPDVDFPAFENKQIEVDAIDVTDSMVDSEIVGIREKLATFTAL
DRPAIDGDFLILDLQILDGDKVLSESNAVPYELGSNTLFDGLDSALTSARAGYTTTIDLTPPVEDPAGENPQQDQFAKSG
DTRRVKLKVSSVNKRDLPEENDELARLVGGFANLEELRKDSAKNIERSMLFQRSLQARDLLLEQLLSEITFPLPGPIADV
LDEIKDEEEKQNRTKQARRDILLDLLAKTFSVTLKEDELERYLSSAAAQRKMSVATLVSVIKSAGQDKYVISDIVRNKAL
SIALSRVKVIDSAGNPLDISEFTIKTDGDPKVRRSE

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation [H]

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain [H]

Homologues:

Organism=Escherichia coli, GI1786640, Length=276, Percent_Identity=27.536231884058, Blast_Score=95, Evalue=1e-20,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881 [H]

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N [H]

EC number: NA

Molecular weight: Translated: 48304; Mature: 48173

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRTLKSIVQRVSETELKLQVTIPSESLKPHVDRAYREVAKTVSVPGFRRGKVPVRIIDQ
CCCHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
RIGKHVVLDRAINDCIGDFFQSAVDEQGLTPVAAPKAHIASLPTEPDASLEVVFDVHVYP
HHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCEEEEEEEEEEC
DVDFPAFENKQIEVDAIDVTDSMVDSEIVGIREKLATFTALDRPAIDGDFLILDLQILDG
CCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECC
DKVLSESNAVPYELGSNTLFDGLDSALTSARAGYTTTIDLTPPVEDPAGENPQQDQFAKS
CHHHCCCCCCCEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHC
GDTRRVKLKVSSVNKRDLPEENDELARLVGGFANLEELRKDSAKNIERSMLFQRSLQARD
CCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LLLEQLLSEITFPLPGPIADVLDEIKDEEEKQNRTKQARRDILLDLLAKTFSVTLKEDEL
HHHHHHHHHCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHEEECHHHH
ERYLSSAAAQRKMSVATLVSVIKSAGQDKYVISDIVRNKALSIALSRVKVIDSAGNPLDI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
SEFTIKTDGDPKVRRSE
CEEEEECCCCCCCCCCC
>Mature Secondary Structure 
GRTLKSIVQRVSETELKLQVTIPSESLKPHVDRAYREVAKTVSVPGFRRGKVPVRIIDQ
CCHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
RIGKHVVLDRAINDCIGDFFQSAVDEQGLTPVAAPKAHIASLPTEPDASLEVVFDVHVYP
HHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCEEEEEEEEEEC
DVDFPAFENKQIEVDAIDVTDSMVDSEIVGIREKLATFTALDRPAIDGDFLILDLQILDG
CCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECC
DKVLSESNAVPYELGSNTLFDGLDSALTSARAGYTTTIDLTPPVEDPAGENPQQDQFAKS
CHHHCCCCCCCEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHC
GDTRRVKLKVSSVNKRDLPEENDELARLVGGFANLEELRKDSAKNIERSMLFQRSLQARD
CCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LLLEQLLSEITFPLPGPIADVLDEIKDEEEKQNRTKQARRDILLDLLAKTFSVTLKEDEL
HHHHHHHHHCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHEEECHHHH
ERYLSSAAAQRKMSVATLVSVIKSAGQDKYVISDIVRNKALSIALSRVKVIDSAGNPLDI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
SEFTIKTDGDPKVRRSE
CEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA