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The map label for this gene is rpoA

Identifier: 28572392

GI number: 28572392

Start: 257011

End: 257994

Strand: Direct

Name: rpoA

Synonym: TW232

Alternate gene names: 28572392

Gene position: 257011-257994 (Clockwise)

Preceding gene: 28572391

Following gene: 28572393

Centisome position: 27.76

GC content: 47.46

Gene sequence:

>984_bases
TTGCTGATTGCCCACCGCCCGACACTGATTGAAAAGAAAGTTTCTGATATTCGCTCGCGATTTATTATTGAGCCTCTTGA
GCCTGGCTTTGGCTATACCCTGGGCAATTCGCTTCGGCGCACACTTCTCTCTTCGATTCCGGGCGCAGCGGTGACATCGA
TAAACATCCAGGGTGTTATGCACGAGTTTAGCACTATTCCAGGTGTTAAGGAAGATGTAACCGAGATTGTTTTAAATGTG
AAGCGTCTGGTTATTTCAAGTGAGATTGACGAGCCGTTTACTGTCCGTCTATATAAAACCGGTGAGGGTGAGGTTTTGGC
AAAGGACATAGAGGTGCCAACCGGCATAGAAATAGGTAATGGTGATCTTGTAATTGCAACCCTCGCCAAAGATGCGGTTT
TTGACATGCAGCTTACAATAGAGCGAGGCAGGGGATATGTTTCAGCGGAACAGAATAGAAATGATGGAATGTCTCTTGCT
GGGCATATTCCGGTAGATTCGATCTATTCTCCTGTTCATAAAGTAACCTATCGCGTTGAGGCTACGCGTGCCGGTGAGAG
GACGGACTTTGATCGCCTGATAATCGATGTCGAGACTAAGCCTTCGATTCTTCCCAGAGATGCAGTTGCAAGTGCAGGGA
AGACTCTTTGCGAGCTGTTTGGCCTTGCACGCGAACTGAATTCTCAGGCCGAGGGTGTTGAGTTTGGTGTTGATTCTATG
ATCCCGGAAAGTGATGAAGATCTGCGTATTCCGATTGAGGACTTGGGGCTCTCGGTAAGATCCTATAACTGCTTGAAGCG
CGAAGGTGTAAATTATGTCAGTGAACTCCTGGGTTTTTCCGAGCAAGAGCTCTTGGATATAAGAAATTTCGGACAGAAAT
CTGCTGATGAGGTCCAGGAGAAGTTAGCTGAGCTGGGTCACAGCCTTAAGGGAAGCGTCCCGGGGTTTGATGGCTCTTAC
TTTGATCCGAATTATGGTTCGTAG

Upstream 100 bases:

>100_bases
AGGTCTTGAGATAGGCTCCATAAGCGACACGACCCCTCTTGCGTTCAATGGATGTCGCCCCCCTAAGAAACGCCTGGTTT
AAAGCGAAAGGAATAATGCG

Downstream 100 bases:

>100_bases
GATTGGGTAGAAAGAGTGCCTAAGCCAAGCAGAGGCCCGCGTATGTGTTCAGGGCCTGACCATGAGCGGCTCGTTCTTGC
GAATATGTCTGCCTCGCTTT

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MLIAHRPTLIEKKVSDIRSRFIIEPLEPGFGYTLGNSLRRTLLSSIPGAAVTSINIQGVMHEFSTIPGVKEDVTEIVLNV
KRLVISSEIDEPFTVRLYKTGEGEVLAKDIEVPTGIEIGNGDLVIATLAKDAVFDMQLTIERGRGYVSAEQNRNDGMSLA
GHIPVDSIYSPVHKVTYRVEATRAGERTDFDRLIIDVETKPSILPRDAVASAGKTLCELFGLARELNSQAEGVEFGVDSM
IPESDEDLRIPIEDLGLSVRSYNCLKREGVNYVSELLGFSEQELLDIRNFGQKSADEVQEKLAELGHSLKGSVPGFDGSY
FDPNYGS

Sequences:

>Translated_327_residues
MLIAHRPTLIEKKVSDIRSRFIIEPLEPGFGYTLGNSLRRTLLSSIPGAAVTSINIQGVMHEFSTIPGVKEDVTEIVLNV
KRLVISSEIDEPFTVRLYKTGEGEVLAKDIEVPTGIEIGNGDLVIATLAKDAVFDMQLTIERGRGYVSAEQNRNDGMSLA
GHIPVDSIYSPVHKVTYRVEATRAGERTDFDRLIIDVETKPSILPRDAVASAGKTLCELFGLARELNSQAEGVEFGVDSM
IPESDEDLRIPIEDLGLSVRSYNCLKREGVNYVSELLGFSEQELLDIRNFGQKSADEVQEKLAELGHSLKGSVPGFDGSY
FDPNYGS
>Mature_327_residues
MLIAHRPTLIEKKVSDIRSRFIIEPLEPGFGYTLGNSLRRTLLSSIPGAAVTSINIQGVMHEFSTIPGVKEDVTEIVLNV
KRLVISSEIDEPFTVRLYKTGEGEVLAKDIEVPTGIEIGNGDLVIATLAKDAVFDMQLTIERGRGYVSAEQNRNDGMSLA
GHIPVDSIYSPVHKVTYRVEATRAGERTDFDRLIIDVETKPSILPRDAVASAGKTLCELFGLARELNSQAEGVEFGVDSM
IPESDEDLRIPIEDLGLSVRSYNCLKREGVNYVSELLGFSEQELLDIRNFGQKSADEVQEKLAELGHSLKGSVPGFDGSY
FDPNYGS

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=310, Percent_Identity=41.2903225806452, Blast_Score=212, Evalue=3e-56,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_TROW8 (Q820D8)

Other databases:

- EMBL:   BX251410
- RefSeq:   NP_789172.1
- ProteinModelPortal:   Q820D8
- STRING:   Q820D8
- GeneID:   1064524
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW232
- eggNOG:   COG0202
- HOGENOM:   HBG430844
- OMA:   FGTTLGN
- ProtClustDB:   PRK05182
- BioCyc:   TWHI218496:TW0217-MONOMER
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 35890; Mature: 35890

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIAHRPTLIEKKVSDIRSRFIIEPLEPGFGYTLGNSLRRTLLSSIPGAAVTSINIQGVM
CEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEHHHHH
HEFSTIPGVKEDVTEIVLNVKRLVISSEIDEPFTVRLYKTGEGEVLAKDIEVPTGIEIGN
HHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEECCCCCCCEEECC
GDLVIATLAKDAVFDMQLTIERGRGYVSAEQNRNDGMSLAGHIPVDSIYSPVHKVTYRVE
CCEEEEEECCCHHEEEEEEEECCCCEEEECCCCCCCCEEECCCCHHHHHHHHHHHEEEEE
ATRAGERTDFDRLIIDVETKPSILPRDAVASAGKTLCELFGLARELNSQAEGVEFGVDSM
CCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECHHHC
IPESDEDLRIPIEDLGLSVRSYNCLKREGVNYVSELLGFSEQELLDIRNFGQKSADEVQE
CCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHH
KLAELGHSLKGSVPGFDGSYFDPNYGS
HHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLIAHRPTLIEKKVSDIRSRFIIEPLEPGFGYTLGNSLRRTLLSSIPGAAVTSINIQGVM
CEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEHHHHH
HEFSTIPGVKEDVTEIVLNVKRLVISSEIDEPFTVRLYKTGEGEVLAKDIEVPTGIEIGN
HHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEECCCCCCCEEECC
GDLVIATLAKDAVFDMQLTIERGRGYVSAEQNRNDGMSLAGHIPVDSIYSPVHKVTYRVE
CCEEEEEECCCHHEEEEEEEECCCCEEEECCCCCCCCEEECCCCHHHHHHHHHHHEEEEE
ATRAGERTDFDRLIIDVETKPSILPRDAVASAGKTLCELFGLARELNSQAEGVEFGVDSM
CCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECHHHC
IPESDEDLRIPIEDLGLSVRSYNCLKREGVNYVSELLGFSEQELLDIRNFGQKSADEVQE
CCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHH
KLAELGHSLKGSVPGFDGSYFDPNYGS
HHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12606174