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The map label for this gene is braC [H]

Identifier: 28572360

GI number: 28572360

Start: 237969

End: 239126

Strand: Direct

Name: braC [H]

Synonym: TW200

Alternate gene names: 28572360

Gene position: 237969-239126 (Clockwise)

Preceding gene: 28572359

Following gene: 28572361

Centisome position: 25.7

GC content: 50.0

Gene sequence:

>1158_bases
TTGTTCTTCAGCGGGTGTTTTGGCTCGGGTGATAATAAGTCGAGTGCGGAACATATAGTCCTTCCAAAAGTCACAGCTCC
TGTCTCGTTTAAGAATGCGCTATTTCCCGCAGGTGATGGTAAGGCGGTGTGTCCTAAGGGTCTTTCTATTGCCTATGTCG
GTATTGTTACCGGTCCGAATGCCGCCTTTGGGAAGAGCATAAATGAGGCGTTTCAACTTGCTGTTAAGCAGCATAACGAC
AATAATCCTGGCTGTCAGGTTACAGGTAAGGTGTTTGACACAGAGGGATCGCCGGATAAAGCTCCCGGAGTTGTCGCGCA
GGTTATTGGAGACTCCTCGATTGTTGCAGTTCTTGGCCCGGCTTTCTCAGGTGAGAACCAGGCTGTTGGGGGTATTTTCG
AAACGGCGCGTCTTGTACACATAACTCCGAGTGCAACCCTGCCAACACTTTCATCTCACGGCTGGAAGACGTTCTTCAGG
GCCGCCTCGACTGATCTCGAGCAGTCTACGGGGGTTATTGCTTTGCTGGAGAAGCTAGAGGCCAAAAAGACTTTTGTTGT
GTCTGATGACAGTGCATACGGTAAATTCCTCGGCGATCTTGTAAAAAAGGGTGTCGATTTGGCTGGAAGCGATGGAATTA
TAACCGGCAGTCGTGATTTTTCCTCTGTTGTCGCGAAGGTTGTTTCTTCCGGCGCCGACTCTGTCTTTTTCGCCGGCTAT
TACCCGGAAGCTGCTGCATTCTTTACGCAACTGAGGGGTGCGGGATTTAATGGTTATTTGGTTGTTCCGGATGGTTCACT
TGATAGAAATCTCAGTAAGCTCGCTGGACAGGCTGCCGTGGGCGTTTACGCGGTTTGCCCTTGCACCGATGACTCGCAGG
TCGCCGGTTTTGGTGATGCAATGAAAAAGGCCTACGGTCACTATCCGGGCATATACTCCTCGAGTGCATACGATGTTACA
ACCATTCTGCTAAGGGGGATAGACTCTGGGCGCACCTCTCGGTCATCCTTACTGGACTGGGTCAGGGGATATGATGCGTA
TGGGGTTTCTGGTCACTACAAGTTTAAGGCAAATGGTGATTTGCAGCACACCCGGCTGTATTTCTATAGGTTTGATGAGT
CAGGTGCTCCTGTATTGGTTGGAGAGTACAGTAAATAA

Upstream 100 bases:

>100_bases
GCGTTTGTATGATACGATCCTAAGAAATCTAGGTAGGCTGAGGTGATGTGTTTTGAATAAGCTTAGGGTTCGTGTTCTGT
TTGCGGCTTCTGCCGCGCTT

Downstream 100 bases:

>100_bases
AAAGGGTACAGTAGATAAAAGCACAACAAACCGGAGTCTGGTCCGGTTTGTTGCTTAGTTTGGATTATCTTTTGAGTCAG
TCGTGGATCACGTTTGATGT

Product: ABC transporter branched chain amino acid binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MFFSGCFGSGDNKSSAEHIVLPKVTAPVSFKNALFPAGDGKAVCPKGLSIAYVGIVTGPNAAFGKSINEAFQLAVKQHND
NNPGCQVTGKVFDTEGSPDKAPGVVAQVIGDSSIVAVLGPAFSGENQAVGGIFETARLVHITPSATLPTLSSHGWKTFFR
AASTDLEQSTGVIALLEKLEAKKTFVVSDDSAYGKFLGDLVKKGVDLAGSDGIITGSRDFSSVVAKVVSSGADSVFFAGY
YPEAAAFFTQLRGAGFNGYLVVPDGSLDRNLSKLAGQAAVGVYAVCPCTDDSQVAGFGDAMKKAYGHYPGIYSSSAYDVT
TILLRGIDSGRTSRSSLLDWVRGYDAYGVSGHYKFKANGDLQHTRLYFYRFDESGAPVLVGEYSK

Sequences:

>Translated_385_residues
MFFSGCFGSGDNKSSAEHIVLPKVTAPVSFKNALFPAGDGKAVCPKGLSIAYVGIVTGPNAAFGKSINEAFQLAVKQHND
NNPGCQVTGKVFDTEGSPDKAPGVVAQVIGDSSIVAVLGPAFSGENQAVGGIFETARLVHITPSATLPTLSSHGWKTFFR
AASTDLEQSTGVIALLEKLEAKKTFVVSDDSAYGKFLGDLVKKGVDLAGSDGIITGSRDFSSVVAKVVSSGADSVFFAGY
YPEAAAFFTQLRGAGFNGYLVVPDGSLDRNLSKLAGQAAVGVYAVCPCTDDSQVAGFGDAMKKAYGHYPGIYSSSAYDVT
TILLRGIDSGRTSRSSLLDWVRGYDAYGVSGHYKFKANGDLQHTRLYFYRFDESGAPVLVGEYSK
>Mature_385_residues
MFFSGCFGSGDNKSSAEHIVLPKVTAPVSFKNALFPAGDGKAVCPKGLSIAYVGIVTGPNAAFGKSINEAFQLAVKQHND
NNPGCQVTGKVFDTEGSPDKAPGVVAQVIGDSSIVAVLGPAFSGENQAVGGIFETARLVHITPSATLPTLSSHGWKTFFR
AASTDLEQSTGVIALLEKLEAKKTFVVSDDSAYGKFLGDLVKKGVDLAGSDGIITGSRDFSSVVAKVVSSGADSVFFAGY
YPEAAAFFTQLRGAGFNGYLVVPDGSLDRNLSKLAGQAAVGVYAVCPCTDDSQVAGFGDAMKKAYGHYPGIYSSSAYDVT
TILLRGIDSGRTSRSSLLDWVRGYDAYGVSGHYKFKANGDLQHTRLYFYRFDESGAPVLVGEYSK

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=328, Percent_Identity=28.6585365853659, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI48994941, Length=325, Percent_Identity=27.0769230769231, Blast_Score=115, Evalue=5e-27,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 40402; Mature: 40402

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFSGCFGSGDNKSSAEHIVLPKVTAPVSFKNALFPAGDGKAVCPKGLSIAYVGIVTGPN
CEECCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCEECCCCCEEEEEEEEECCC
AAFGKSINEAFQLAVKQHNDNNPGCQVTGKVFDTEGSPDKAPGVVAQVIGDSSIVAVLGP
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHCCCCEEEEECC
AFSGENQAVGGIFETARLVHITPSATLPTLSSHGWKTFFRAASTDLEQSTGVIALLEKLE
CCCCCCCCCCHHHHCCEEEEECCCCCCCCCCCCCHHHHHHHHCCCHHHHCCHHHHHHHHC
AKKTFVVSDDSAYGKFLGDLVKKGVDLAGSDGIITGSRDFSSVVAKVVSSGADSVFFAGY
CCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCEEECCCHHHHHHHHHHHCCCCCEEEEEE
YPEAAAFFTQLRGAGFNGYLVVPDGSLDRNLSKLAGQAAVGVYAVCPCTDDSQVAGFGDA
CCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHH
MKKAYGHYPGIYSSSAYDVTTILLRGIDSGRTSRSSLLDWVRGYDAYGVSGHYKFKANGD
HHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCC
LQHTRLYFYRFDESGAPVLVGEYSK
CEEEEEEEEEECCCCCCEEEECCCC
>Mature Secondary Structure
MFFSGCFGSGDNKSSAEHIVLPKVTAPVSFKNALFPAGDGKAVCPKGLSIAYVGIVTGPN
CEECCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCEECCCCCEEEEEEEEECCC
AAFGKSINEAFQLAVKQHNDNNPGCQVTGKVFDTEGSPDKAPGVVAQVIGDSSIVAVLGP
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHCCCCEEEEECC
AFSGENQAVGGIFETARLVHITPSATLPTLSSHGWKTFFRAASTDLEQSTGVIALLEKLE
CCCCCCCCCCHHHHCCEEEEECCCCCCCCCCCCCHHHHHHHHCCCHHHHCCHHHHHHHHC
AKKTFVVSDDSAYGKFLGDLVKKGVDLAGSDGIITGSRDFSSVVAKVVSSGADSVFFAGY
CCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCEEECCCHHHHHHHHHHHCCCCCEEEEEE
YPEAAAFFTQLRGAGFNGYLVVPDGSLDRNLSKLAGQAAVGVYAVCPCTDDSQVAGFGDA
CCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHH
MKKAYGHYPGIYSSSAYDVTTILLRGIDSGRTSRSSLLDWVRGYDAYGVSGHYKFKANGD
HHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCC
LQHTRLYFYRFDESGAPVLVGEYSK
CEEEEEEEEEECCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]