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The map label for this gene is recG [H]

Identifier: 28572355

GI number: 28572355

Start: 232840

End: 234861

Strand: Direct

Name: recG [H]

Synonym: TW195

Alternate gene names: 28572355

Gene position: 232840-234861 (Clockwise)

Preceding gene: 28572353

Following gene: 28572356

Centisome position: 25.15

GC content: 47.28

Gene sequence:

>2022_bases
ATGGCATCACTATTAGCCAAAGAGTTTGGTATTAGTCGGGTGATAGACCTGCTCACATATTACCCTCGAAGGTACATCTG
CAGGGGTAAGCTTACTAAGCTTTCAGAGCTAATTCCCGGAGACGAGGTTACGATTGTTGGCCGGGTTCTAAGCACCGAGC
AGCGTAAGACATTCAGTGGAGCGAATTTTCTCAGCGTTACGCTCAGTGATGGAGAGAATATCATTCAACTTGTTTTCTTT
CACCAACCCTGGCGCGCAGAAAACTTGAAACCCGGTGCATGTGGGCTATTTTCGGGAAAGGTAACAGAGTTCAATAACAA
GAAGCAACTCTCACATCCCGAATATGAATTATTTTCATCAGAGCCAACAAGGGAACAGATACAGAAGTGGAATGAGCAGA
TAATACCGATTTATCGCGCTTGCATCGCGTGTCCAAGCTGGAAAATAGCCCGGGCTGTTGATTTGGCTTTAGAAGCGGTA
AAGGGCCAAACTGTAGATTTTATGTCGGGTAATTATGGCTATATGTCAGTTGAAAAGGCGTTTTATGTTATTCATCATCC
TACGGACAACGAAGAGCTTGAGGCCGCAAAAGAAAGTTTGCGCTTTTTTGAGGCATTTTTGTTACAAAGCGCCCTTCTAC
ACCGAAAGCGGTTCCGCAATAGAGCAAGCGCAACACCTTTTATTCGTAAAAATGGCGGTTTTTTAGAGAGATTTGATGCG
AGGCTGGAGTTTTCTCAGACTAATGATCAGTTGCGCGCTGCAGATGAAATTTTCGAAGACTTGTCGCTGTCAGAGCCAAT
GACCCGTCTCTTACATGGTGAGGTCGGTAGCGGAAAAACTCTGGTTGCGATAAGAGCTATGCTCCTTGCGGCAGATAACG
ACATGCAGGCCGTCCTCGTTGCGCCTACCGAAGTTCTTGCAAAACAGCATCACAGGAATTTAACTCGCATGCTGGGCCAC
GAGCTATGTGCCGAGATACAGCCATCTCTCTTGCTTGGTAGGGAAAAACACACCTTGCGTATTGCCTCAGGCAGAAGCAA
GATCATTATAGGAACACATTCGGTCTTTAGTAAAAAAACCGTATTTCATAATCTCGCCCTTGTTGTTATCGATGAGCAGC
ACCGCTTTGGCGTTGGCCAGCGGGACGAATTACTTTTGAAAGGGGATTCACCTCATTTGCTTATGCTGTCTGCAACACCA
ATTCCACGAACAGTTGCCCTTTCTCTGCTGAATTTTATAGCAATAAGCGAGATAAAGACACCACCAAGCGGTAAGGCTGA
AATTTCGACGCATGTTGTACCCCTTGCAGAAAAGCCTCAATGGGGAAGGGAGGTTATAAGGAGGATTTCCGAGGAAATAC
AAAAAGGTCACCAGGTTTTTGTTGTTGCGCCCGTTATAGAACAAAGCCGTACGGGCGCGGCCAGCATAAGTGCCTTGCTA
AGGGAGCTTGAGGAAACCCCTCTCCTTCAAGGCGCGAAGATCTCCCGACTGCATGGCAAGTTGACCGCTGCGGAGTGCGA
GAAGTCTATGGAAGAGTTCTCTTGCGGGGCCAGCGATATTCTGCTCTCAACAACCATGATTGAGGTGGGTATAGATGTTC
CAAATGCCACGGCAATGGTTATTGTGTCAGCCGATCGCTTTGGTATCGCTCAGTTGCACCAGTTGCGCGGACGGATCGGT
CGAGGTAATTTGCCCGGCGTGTGTCTTCTTGTTACTAATGCCCCCGAGGGCTCGGCGGCGCGCTCTAGGTTGGATTTAGT
TGCAAGAACACATGACGGATTTTCTCTTGCGGAAATCGATATGAAAATGAGGAGAGAGGGAGATTTGCTCGGCCTGGGTC
AATCTGGGCAGGGTAATTATCGCCTTTTACGCCTTGATGAGGACCTGCAGGTTTTATCGGACGCCCGATTACACGCGGAG
GGAATAATGGAGAATGACATAAAACTCGAGAAGAATAAGCTTCTTCGATTGTTTTTGTCTCAATATTTGTCGGGCACTAA
TTTGAGTCGATTGCTTTCATAA

Upstream 100 bases:

>100_bases
TGGTTGGTACATTTCAGTTTTCGCCATCTTGTTGCCCACTAAAATTGTAGTAATGGAGCATGTTTTTTTCAGTGATGTGT
CTGTCCACACTGGCAAAAAA

Downstream 100 bases:

>100_bases
GAAATTTATGTATTGAGCAATTTATCCTGTACTATCATCTCCGCCGACCACCTGATTTTAGAGTCAGTTATCTAGCTGTG
TGCGATTTTATATCTAGATG

Product: ATP-dependent DNA helicase RecG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 673; Mature: 672

Protein sequence:

>673_residues
MASLLAKEFGISRVIDLLTYYPRRYICRGKLTKLSELIPGDEVTIVGRVLSTEQRKTFSGANFLSVTLSDGENIIQLVFF
HQPWRAENLKPGACGLFSGKVTEFNNKKQLSHPEYELFSSEPTREQIQKWNEQIIPIYRACIACPSWKIARAVDLALEAV
KGQTVDFMSGNYGYMSVEKAFYVIHHPTDNEELEAAKESLRFFEAFLLQSALLHRKRFRNRASATPFIRKNGGFLERFDA
RLEFSQTNDQLRAADEIFEDLSLSEPMTRLLHGEVGSGKTLVAIRAMLLAADNDMQAVLVAPTEVLAKQHHRNLTRMLGH
ELCAEIQPSLLLGREKHTLRIASGRSKIIIGTHSVFSKKTVFHNLALVVIDEQHRFGVGQRDELLLKGDSPHLLMLSATP
IPRTVALSLLNFIAISEIKTPPSGKAEISTHVVPLAEKPQWGREVIRRISEEIQKGHQVFVVAPVIEQSRTGAASISALL
RELEETPLLQGAKISRLHGKLTAAECEKSMEEFSCGASDILLSTTMIEVGIDVPNATAMVIVSADRFGIAQLHQLRGRIG
RGNLPGVCLLVTNAPEGSAARSRLDLVARTHDGFSLAEIDMKMRREGDLLGLGQSGQGNYRLLRLDEDLQVLSDARLHAE
GIMENDIKLEKNKLLRLFLSQYLSGTNLSRLLS

Sequences:

>Translated_673_residues
MASLLAKEFGISRVIDLLTYYPRRYICRGKLTKLSELIPGDEVTIVGRVLSTEQRKTFSGANFLSVTLSDGENIIQLVFF
HQPWRAENLKPGACGLFSGKVTEFNNKKQLSHPEYELFSSEPTREQIQKWNEQIIPIYRACIACPSWKIARAVDLALEAV
KGQTVDFMSGNYGYMSVEKAFYVIHHPTDNEELEAAKESLRFFEAFLLQSALLHRKRFRNRASATPFIRKNGGFLERFDA
RLEFSQTNDQLRAADEIFEDLSLSEPMTRLLHGEVGSGKTLVAIRAMLLAADNDMQAVLVAPTEVLAKQHHRNLTRMLGH
ELCAEIQPSLLLGREKHTLRIASGRSKIIIGTHSVFSKKTVFHNLALVVIDEQHRFGVGQRDELLLKGDSPHLLMLSATP
IPRTVALSLLNFIAISEIKTPPSGKAEISTHVVPLAEKPQWGREVIRRISEEIQKGHQVFVVAPVIEQSRTGAASISALL
RELEETPLLQGAKISRLHGKLTAAECEKSMEEFSCGASDILLSTTMIEVGIDVPNATAMVIVSADRFGIAQLHQLRGRIG
RGNLPGVCLLVTNAPEGSAARSRLDLVARTHDGFSLAEIDMKMRREGDLLGLGQSGQGNYRLLRLDEDLQVLSDARLHAE
GIMENDIKLEKNKLLRLFLSQYLSGTNLSRLLS
>Mature_672_residues
ASLLAKEFGISRVIDLLTYYPRRYICRGKLTKLSELIPGDEVTIVGRVLSTEQRKTFSGANFLSVTLSDGENIIQLVFFH
QPWRAENLKPGACGLFSGKVTEFNNKKQLSHPEYELFSSEPTREQIQKWNEQIIPIYRACIACPSWKIARAVDLALEAVK
GQTVDFMSGNYGYMSVEKAFYVIHHPTDNEELEAAKESLRFFEAFLLQSALLHRKRFRNRASATPFIRKNGGFLERFDAR
LEFSQTNDQLRAADEIFEDLSLSEPMTRLLHGEVGSGKTLVAIRAMLLAADNDMQAVLVAPTEVLAKQHHRNLTRMLGHE
LCAEIQPSLLLGREKHTLRIASGRSKIIIGTHSVFSKKTVFHNLALVVIDEQHRFGVGQRDELLLKGDSPHLLMLSATPI
PRTVALSLLNFIAISEIKTPPSGKAEISTHVVPLAEKPQWGREVIRRISEEIQKGHQVFVVAPVIEQSRTGAASISALLR
ELEETPLLQGAKISRLHGKLTAAECEKSMEEFSCGASDILLSTTMIEVGIDVPNATAMVIVSADRFGIAQLHQLRGRIGR
GNLPGVCLLVTNAPEGSAARSRLDLVARTHDGFSLAEIDMKMRREGDLLGLGQSGQGNYRLLRLDEDLQVLSDARLHAEG
IMENDIKLEKNKLLRLFLSQYLSGTNLSRLLS

Specific function: Critical role in recombination and DNA repair. Help process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3' to 5' polarity. RecG unwind branched dup

COG id: COG1200

COG function: function code LK; RecG-like helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367254, Length=670, Percent_Identity=33.134328358209, Blast_Score=299, Evalue=4e-82,
Organism=Escherichia coli, GI1787357, Length=382, Percent_Identity=35.0785340314136, Blast_Score=217, Evalue=2e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR004609
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C; PF01336 tRNA_anti [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 74952; Mature: 74820

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASLLAKEFGISRVIDLLTYYPRRYICRGKLTKLSELIPGDEVTIVGRVLSTEQRKTFSG
CCHHHHHHHCHHHHHHHHHHCCHHHHHCCHHHHHHHHCCCCCEEEEEHHHCCHHHHHCCC
ANFLSVTLSDGENIIQLVFFHQPWRAENLKPGACGLFSGKVTEFNNKKQLSHPEYELFSS
CCEEEEEECCCCCEEEEEEEECCCCCCCCCCCCCEECCCCEECCCCCCCCCCCCHHHCCC
EPTREQIQKWNEQIIPIYRACIACPSWKIARAVDLALEAVKGQTVDFMSGNYGYMSVEKA
CCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEEE
FYVIHHPTDNEELEAAKESLRFFEAFLLQSALLHRKRFRNRASATPFIRKNGGFLERFDA
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHC
RLEFSQTNDQLRAADEIFEDLSLSEPMTRLLHGEVGSGKTLVAIRAMLLAADNDMQAVLV
EEEECCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEEEE
APTEVLAKQHHRNLTRMLGHELCAEIQPSLLLGREKHTLRIASGRSKIIIGTHSVFSKKT
CCHHHHHHHHHHHHHHHHHHHHHHHCCCHHEECCCCCEEEEECCCCEEEEECCHHHHHHH
VFHNLALVVIDEQHRFGVGQRDELLLKGDSPHLLMLSATPIPRTVALSLLNFIAISEIKT
HHHCEEEEEEECHHHCCCCCCCCEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCC
PPSGKAEISTHVVPLAEKPQWGREVIRRISEEIQKGHQVFVVAPVIEQSRTGAASISALL
CCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEHHCCCCCHHHHHHHH
RELEETPLLQGAKISRLHGKLTAAECEKSMEEFSCGASDILLSTTMIEVGIDVPNATAMV
HHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHEEEEEECCCCCCEEEE
IVSADRFGIAQLHQLRGRIGRGNLPGVCLLVTNAPEGSAARSRLDLVARTHDGFSLAEID
EEECCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHEECCCCCEEHHHH
MKMRREGDLLGLGQSGQGNYRLLRLDEDLQVLSDARLHAEGIMENDIKLEKNKLLRLFLS
HHHHCCCCEEECCCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHH
QYLSGTNLSRLLS
HHHCCCCHHHHCC
>Mature Secondary Structure 
ASLLAKEFGISRVIDLLTYYPRRYICRGKLTKLSELIPGDEVTIVGRVLSTEQRKTFSG
CHHHHHHHCHHHHHHHHHHCCHHHHHCCHHHHHHHHCCCCCEEEEEHHHCCHHHHHCCC
ANFLSVTLSDGENIIQLVFFHQPWRAENLKPGACGLFSGKVTEFNNKKQLSHPEYELFSS
CCEEEEEECCCCCEEEEEEEECCCCCCCCCCCCCEECCCCEECCCCCCCCCCCCHHHCCC
EPTREQIQKWNEQIIPIYRACIACPSWKIARAVDLALEAVKGQTVDFMSGNYGYMSVEKA
CCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEEE
FYVIHHPTDNEELEAAKESLRFFEAFLLQSALLHRKRFRNRASATPFIRKNGGFLERFDA
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHC
RLEFSQTNDQLRAADEIFEDLSLSEPMTRLLHGEVGSGKTLVAIRAMLLAADNDMQAVLV
EEEECCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEEEE
APTEVLAKQHHRNLTRMLGHELCAEIQPSLLLGREKHTLRIASGRSKIIIGTHSVFSKKT
CCHHHHHHHHHHHHHHHHHHHHHHHCCCHHEECCCCCEEEEECCCCEEEEECCHHHHHHH
VFHNLALVVIDEQHRFGVGQRDELLLKGDSPHLLMLSATPIPRTVALSLLNFIAISEIKT
HHHCEEEEEEECHHHCCCCCCCCEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCC
PPSGKAEISTHVVPLAEKPQWGREVIRRISEEIQKGHQVFVVAPVIEQSRTGAASISALL
CCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEHHCCCCCHHHHHHHH
RELEETPLLQGAKISRLHGKLTAAECEKSMEEFSCGASDILLSTTMIEVGIDVPNATAMV
HHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHEEEEEECCCCCCEEEE
IVSADRFGIAQLHQLRGRIGRGNLPGVCLLVTNAPEGSAARSRLDLVARTHDGFSLAEID
EEECCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHEECCCCCEEHHHH
MKMRREGDLLGLGQSGQGNYRLLRLDEDLQVLSDARLHAEGIMENDIKLEKNKLLRLFLS
HHHHCCCCEEECCCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHH
QYLSGTNLSRLLS
HHHCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]