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The map label for this gene is tilS [H]

Identifier: 28572333

GI number: 28572333

Start: 212254

End: 213477

Strand: Direct

Name: tilS [H]

Synonym: TW171

Alternate gene names: 28572333

Gene position: 212254-213477 (Clockwise)

Preceding gene: 28572332

Following gene: 28572334

Centisome position: 22.92

GC content: 47.3

Gene sequence:

>1224_bases
GTGTTGCACAGGCCCCGCATAACTCCTGCAGTTGCAAATGCACGGCGCGCTATGCTAAACACCCTTCCAGCGGATTTGCA
TAATCAACTCGTTTTGATTGCTCTTTCTGGCGGGAGAGACTCGCTGGCCGCTATGGTAATCGCGTCATGGGTTATTCCGC
GCCTGAACGGACGCGTTGGTGCCGTTGTGGTAGACCATGGTTTGCAGGAGAATTCTGCACGCATTGCTGACGAAGTCAGA
ATACGGGCAGAGCAGTTTGCACTAAATCCCATTCTGATAAAGAGAGTCTCGCCCTCGAGAGTATCTGCTGGGCCAGAGGC
GTCTGCCCGCATTGCACGCTATGCTGCCTTTTATGATACTCTTGAAGAAACTAAAGCTTATGCGATTATCCTTGCCCATA
CACTCGATGATCAAGCAGAAACGGTTTTACTCGGCCTCATGCGCGGTTCGGGCCCGTCAAGTTTGCGAGGTATGAAGGCT
GTATCGTACACTCCGGAAGATTGCAGATACATGAATAATAAGGCGTGTAATTCCGTCACCGCCGTGTATGCCCACAATAC
ACAGCGGTGTTCATGTGACTCACGCCCGTACCCACCGCCTCCCGAACCGCGCAATGAGAACAGAAGTTATTTTCCGCACC
CATCTTGTAAATGCGTGGAGGGTTCCGCAACGCGCTTCACAAACGCAGATATAGATTCGCGCTTTGGCGTGTTTATAAGG
CCATTTTTGGATATTACCCGGCATGAAACAGGTAAGATTTGTGAGTTCTATGGGCTCGATTACTGGAATGATCCTCATAA
TGAGGATGTGCGTTTTTCGCGTGTTCGGATTCGTCACAATGTTATGCCGGTTTTGGAAAATGAAATTGGACCGGGTGTTA
AATACGCGCTATCGCGAACCGCTAAGTTGGCACAATTGGATACTGAATATCTTGATCATTTATCAAATGAATTGCTCGAT
GAGATAGCGTCTAAAGAATCAGATTGGTCAATTAGGCTGCCGATTAATACTCTTCAAAAAACCCCAGTACCTATTCGACT
TAGGGTTATACGACTTGCTGCCCTACAGTATTTTACTGTTAGTTTGAGTTTTAAACACACCCGTCAAATAGAGAGACTAC
TGTGCTCATCTTGTGACATAAAGCATGTAAACCTGCCACGGTTAATTACGGCACAGAGGGTTGGTAATTACATTTACATG
CACACCTTACGCGGTAAACTGTAA

Upstream 100 bases:

>100_bases
TACATATACCGACACCCTTACATATACCGCATATCGTGACCTCTCTACCACTGCTAGCCGTACTATACACTCATATGTCA
ACACGGTACACTGAAGTACC

Downstream 100 bases:

>100_bases
GCATATGAAGCCGCTTTTATCAGAAGCAGTAATCCATAGGAGACTTGCACAGCTCGCTTTGCAGATAGACGAGGATTATC
GTGGAACCTCGATTGTTCTG

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MLHRPRITPAVANARRAMLNTLPADLHNQLVLIALSGGRDSLAAMVIASWVIPRLNGRVGAVVVDHGLQENSARIADEVR
IRAEQFALNPILIKRVSPSRVSAGPEASARIARYAAFYDTLEETKAYAIILAHTLDDQAETVLLGLMRGSGPSSLRGMKA
VSYTPEDCRYMNNKACNSVTAVYAHNTQRCSCDSRPYPPPPEPRNENRSYFPHPSCKCVEGSATRFTNADIDSRFGVFIR
PFLDITRHETGKICEFYGLDYWNDPHNEDVRFSRVRIRHNVMPVLENEIGPGVKYALSRTAKLAQLDTEYLDHLSNELLD
EIASKESDWSIRLPINTLQKTPVPIRLRVIRLAALQYFTVSLSFKHTRQIERLLCSSCDIKHVNLPRLITAQRVGNYIYM
HTLRGKL

Sequences:

>Translated_407_residues
MLHRPRITPAVANARRAMLNTLPADLHNQLVLIALSGGRDSLAAMVIASWVIPRLNGRVGAVVVDHGLQENSARIADEVR
IRAEQFALNPILIKRVSPSRVSAGPEASARIARYAAFYDTLEETKAYAIILAHTLDDQAETVLLGLMRGSGPSSLRGMKA
VSYTPEDCRYMNNKACNSVTAVYAHNTQRCSCDSRPYPPPPEPRNENRSYFPHPSCKCVEGSATRFTNADIDSRFGVFIR
PFLDITRHETGKICEFYGLDYWNDPHNEDVRFSRVRIRHNVMPVLENEIGPGVKYALSRTAKLAQLDTEYLDHLSNELLD
EIASKESDWSIRLPINTLQKTPVPIRLRVIRLAALQYFTVSLSFKHTRQIERLLCSSCDIKHVNLPRLITAQRVGNYIYM
HTLRGKL
>Mature_407_residues
MLHRPRITPAVANARRAMLNTLPADLHNQLVLIALSGGRDSLAAMVIASWVIPRLNGRVGAVVVDHGLQENSARIADEVR
IRAEQFALNPILIKRVSPSRVSAGPEASARIARYAAFYDTLEETKAYAIILAHTLDDQAETVLLGLMRGSGPSSLRGMKA
VSYTPEDCRYMNNKACNSVTAVYAHNTQRCSCDSRPYPPPPEPRNENRSYFPHPSCKCVEGSATRFTNADIDSRFGVFIR
PFLDITRHETGKICEFYGLDYWNDPHNEDVRFSRVRIRHNVMPVLENEIGPGVKYALSRTAKLAQLDTEYLDHLSNELLD
EIASKESDWSIRLPINTLQKTPVPIRLRVIRLAALQYFTVSLSFKHTRQIERLLCSSCDIKHVNLPRLITAQRVGNYIYM
HTLRGKL

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=138, Percent_Identity=36.231884057971, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 45844; Mature: 45844

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHRPRITPAVANARRAMLNTLPADLHNQLVLIALSGGRDSLAAMVIASWVIPRLNGRVG
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCEE
AVVVDHGLQENSARIADEVRIRAEQFALNPILIKRVSPSRVSAGPEASARIARYAAFYDT
EEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHH
LEETKAYAIILAHTLDDQAETVLLGLMRGSGPSSLRGMKAVSYTPEDCRYMNNKACNSVT
HHHCCEEEEEEEECCCCCHHHHHHHHHCCCCCCHHCCCEEECCCCHHHHHHCCCCCCCEE
AVYAHNTQRCSCDSRPYPPPPEPRNENRSYFPHPSCKCVEGSATRFTNADIDSRFGVFIR
EEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCEECCCCCCCCCCHHHH
PFLDITRHETGKICEFYGLDYWNDPHNEDVRFSRVRIRHNVMPVLENEIGPGVKYALSRT
HHHHHHCCCCCCEEEEECCCCCCCCCCCCCEEHEEEHHHCCHHHHHHCCCCCHHHHHHHH
AKLAQLDTEYLDHLSNELLDEIASKESDWSIRLPINTLQKTPVPIRLRVIRLAALQYFTV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHCCCCCHHHHHHHHHHHEEEEE
SLSFKHTRQIERLLCSSCDIKHVNLPRLITAQRVGNYIYMHTLRGKL
EEEHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHCCEEEEEECCCCC
>Mature Secondary Structure
MLHRPRITPAVANARRAMLNTLPADLHNQLVLIALSGGRDSLAAMVIASWVIPRLNGRVG
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCEE
AVVVDHGLQENSARIADEVRIRAEQFALNPILIKRVSPSRVSAGPEASARIARYAAFYDT
EEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHH
LEETKAYAIILAHTLDDQAETVLLGLMRGSGPSSLRGMKAVSYTPEDCRYMNNKACNSVT
HHHCCEEEEEEEECCCCCHHHHHHHHHCCCCCCHHCCCEEECCCCHHHHHHCCCCCCCEE
AVYAHNTQRCSCDSRPYPPPPEPRNENRSYFPHPSCKCVEGSATRFTNADIDSRFGVFIR
EEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCEECCCCCCCCCCHHHH
PFLDITRHETGKICEFYGLDYWNDPHNEDVRFSRVRIRHNVMPVLENEIGPGVKYALSRT
HHHHHHCCCCCCEEEEECCCCCCCCCCCCCEEHEEEHHHCCHHHHHHCCCCCHHHHHHHH
AKLAQLDTEYLDHLSNELLDEIASKESDWSIRLPINTLQKTPVPIRLRVIRLAALQYFTV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHCCCCCHHHHHHHHHHHEEEEE
SLSFKHTRQIERLLCSSCDIKHVNLPRLITAQRVGNYIYMHTLRGKL
EEEHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12902375 [H]