Click here to switch to the map view.

The map label for this gene is menD [H]

Identifier: 28572246

GI number: 28572246

Start: 70637

End: 72220

Strand: Direct

Name: menD [H]

Synonym: TW078

Alternate gene names: 28572246

Gene position: 70637-72220 (Clockwise)

Preceding gene: 28572245

Following gene: 28572247

Centisome position: 7.63

GC content: 49.05

Gene sequence:

>1584_bases
ATGACATGTGCGTCTGGGATTGCTTTTTGCTTGCTAAATCTACTTGCCGAGCGCGGTGTGCGTGATATTGTTGTCGCTCC
GGGAGCAAGATCCCAGGCACTCGCGCTTGCCGCGGTTGCTCTAGAGCGTGCAGGGCTGGTAAGGCTTACTGTCCGCATTG
ATGAAAGAGTTGCTGCTTTTACCGCCTTTGGAATGGCCAAGGCTCTTGATTCCCCTGCTGTTGTTATAACAACAAGTGGG
AGTGCTGTGGCAAATTTGTACCCTGCTGTTGTTGAAGCCTCTCATTCATTTGTTCCGCTGATTGTTATAACGGCTGACCG
CCCGCGGGAATTGCACGGTAAAAGGTCTAATCAAACAATGAATCAAGAGAAATTTTTTTCTCACTGGGTCAGATTTTTTT
GCCATATTGAACAGGATGTACCCGGTGAAAGAGTTATAGATAGTGCCTTACAGGCAGCACTTGGTGTGTCGAGTTTACGA
GGCGCAGGTCCTGGACCCGTGCATATAAATGTTGCCTTTGACAATCCACTTTCGTGCGCACATGCGGTGCAGCCCGGGGA
CAATAAAGGCAGTGCACTAAAGCACAAAAAAGTCCTTCGCAGCAAGAGAGAAACACTGGTCCTCAGAAAGGACGATCCAC
CGACGGTTATTATTGCCGGGACTGGCTCGGGCAGAAGGCCTGCGGATATAGCCTGCGCAACTGGCTGGCCTCTGGTTGCT
GAGATAAGCAGTGGATCACGCTTTGGGCCGTTCCTGATAAAAAACTATCGTAATTGGCTCACTGAGAACAGCTTAGATAT
CAGACGTGCAATCGTTTTTGGCCACCCAACGCTCAGTAGCGAAATTCCCACTTTTTTAACGAAGAGAAACATTGACACCA
TTGTGGTTGCACCTTTTGGGCGTGAGTTTTATAACCCGTCAGGAACTGCCCGACCCGTAGCCAATGTTCTTGTTGATGAG
GACAGTTTTTCTGGAATCCCGCTTGGGGCAGCTAAGTTTGCCTCGGCATCAGCAGGCTGCATATCGCAGGATGTAATCTC
CTCAGACAGACCGCGCGGACACCGTGTCACTCGACAACAACTTGTTAGCGAGATTTGGAATATAACCAAAGAAGACGAAA
TATTGTTTTTTGGATCATCACAACTCATAAGGGTCGCAGACTCTATCTTGCCCGGCAAGAGAGTTACCGTTTTTGCCAAT
CGTGGGTTGTCAGGGATAGACGGTAATCTGTCAACGGCAGCTGGTATCGCCATCGCCACAAATCGTACAACTCGAGTTCT
CGTTGGTGACCTTACCGGTGTGCATGATATTGGCGCTCTGCTCATCCCCCTTATGGAGGCGAAATACAGGCTGCAGATTG
TCATTGGGAACGACTTCGGTGGCGGTATTTTTGACACCCTGGAAGTGCATGCAACGCCATCGGATTACAAGAGGGTTCTT
TATGTTCCGTGTAGAGTCGATTTTAGGCTCGTATCTCATGCATTTGGTTGGAAGTACACGTTTTGTCCGGATCCGAACAG
TTTGCGCAAAGCTCTGAAATCGCGGATTTTTCCGCATTTGCTTGAGGTCCATCTTGCTGTTTAA

Upstream 100 bases:

>100_bases
ACTATCTTGACTTGCGAGCGGCACATTTGAAAAAGCTGCGCGATGAGCGTGGCACACAGCCTTGACGCTTGTAATCGATG
CGAAACACCTTGATTAGCCT

Downstream 100 bases:

>100_bases
AATGCGGATATGAAAAGGGTTTATCTTCGTCGACGTATTATGCTATCGATGCTAATTGTTCTGCCTTTGATTGTTGCGGT
CTTTTTTGCTACAGGATCTC

Product: menaquinone biosynthesis protein MenD

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD [H]

Number of amino acids: Translated: 527; Mature: 526

Protein sequence:

>527_residues
MTCASGIAFCLLNLLAERGVRDIVVAPGARSQALALAAVALERAGLVRLTVRIDERVAAFTAFGMAKALDSPAVVITTSG
SAVANLYPAVVEASHSFVPLIVITADRPRELHGKRSNQTMNQEKFFSHWVRFFCHIEQDVPGERVIDSALQAALGVSSLR
GAGPGPVHINVAFDNPLSCAHAVQPGDNKGSALKHKKVLRSKRETLVLRKDDPPTVIIAGTGSGRRPADIACATGWPLVA
EISSGSRFGPFLIKNYRNWLTENSLDIRRAIVFGHPTLSSEIPTFLTKRNIDTIVVAPFGREFYNPSGTARPVANVLVDE
DSFSGIPLGAAKFASASAGCISQDVISSDRPRGHRVTRQQLVSEIWNITKEDEILFFGSSQLIRVADSILPGKRVTVFAN
RGLSGIDGNLSTAAGIAIATNRTTRVLVGDLTGVHDIGALLIPLMEAKYRLQIVIGNDFGGGIFDTLEVHATPSDYKRVL
YVPCRVDFRLVSHAFGWKYTFCPDPNSLRKALKSRIFPHLLEVHLAV

Sequences:

>Translated_527_residues
MTCASGIAFCLLNLLAERGVRDIVVAPGARSQALALAAVALERAGLVRLTVRIDERVAAFTAFGMAKALDSPAVVITTSG
SAVANLYPAVVEASHSFVPLIVITADRPRELHGKRSNQTMNQEKFFSHWVRFFCHIEQDVPGERVIDSALQAALGVSSLR
GAGPGPVHINVAFDNPLSCAHAVQPGDNKGSALKHKKVLRSKRETLVLRKDDPPTVIIAGTGSGRRPADIACATGWPLVA
EISSGSRFGPFLIKNYRNWLTENSLDIRRAIVFGHPTLSSEIPTFLTKRNIDTIVVAPFGREFYNPSGTARPVANVLVDE
DSFSGIPLGAAKFASASAGCISQDVISSDRPRGHRVTRQQLVSEIWNITKEDEILFFGSSQLIRVADSILPGKRVTVFAN
RGLSGIDGNLSTAAGIAIATNRTTRVLVGDLTGVHDIGALLIPLMEAKYRLQIVIGNDFGGGIFDTLEVHATPSDYKRVL
YVPCRVDFRLVSHAFGWKYTFCPDPNSLRKALKSRIFPHLLEVHLAV
>Mature_526_residues
TCASGIAFCLLNLLAERGVRDIVVAPGARSQALALAAVALERAGLVRLTVRIDERVAAFTAFGMAKALDSPAVVITTSGS
AVANLYPAVVEASHSFVPLIVITADRPRELHGKRSNQTMNQEKFFSHWVRFFCHIEQDVPGERVIDSALQAALGVSSLRG
AGPGPVHINVAFDNPLSCAHAVQPGDNKGSALKHKKVLRSKRETLVLRKDDPPTVIIAGTGSGRRPADIACATGWPLVAE
ISSGSRFGPFLIKNYRNWLTENSLDIRRAIVFGHPTLSSEIPTFLTKRNIDTIVVAPFGREFYNPSGTARPVANVLVDED
SFSGIPLGAAKFASASAGCISQDVISSDRPRGHRVTRQQLVSEIWNITKEDEILFFGSSQLIRVADSILPGKRVTVFANR
GLSGIDGNLSTAAGIAIATNRTTRVLVGDLTGVHDIGALLIPLMEAKYRLQIVIGNDFGGGIFDTLEVHATPSDYKRVLY
VPCRVDFRLVSHAFGWKYTFCPDPNSLRKALKSRIFPHLLEVHLAV

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788599, Length=253, Percent_Identity=35.5731225296443, Blast_Score=102, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004433
- InterPro:   IPR012001 [H]

Pfam domain/function: PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.9 [H]

Molecular weight: Translated: 56965; Mature: 56834

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTCASGIAFCLLNLLAERGVRDIVVAPGARSQALALAAVALERAGLVRLTVRIDERVAAF
CCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCEEEEEEEECHHHHHH
TAFGMAKALDSPAVVITTSGSAVANLYPAVVEASHSFVPLIVITADRPRELHGKRSNQTM
HHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCEEEEEEEECCCCHHHCCCCCCCCC
NQEKFFSHWVRFFCHIEQDVPGERVIDSALQAALGVSSLRGAGPGPVHINVAFDNPLSCA
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHCCCCCCCEEEEEEECCCCCHH
HAVQPGDNKGSALKHKKVLRSKRETLVLRKDDPPTVIIAGTGSGRRPADIACATGWPLVA
HCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCEEEEECCCCCCCCCCEEEECCCCEEE
EISSGSRFGPFLIKNYRNWLTENSLDIRRAIVFGHPTLSSEIPTFLTKRNIDTIVVAPFG
EECCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCHHHHHCCCCEEEEECCC
REFYNPSGTARPVANVLVDEDSFSGIPLGAAKFASASAGCISQDVISSDRPRGHRVTRQQ
HHHCCCCCCCCHHHHEEECCCCCCCCCCCHHHHCCCCCCCCHHHHHCCCCCCCCHHHHHH
LVSEIWNITKEDEILFFGSSQLIRVADSILPGKRVTVFANRGLSGIDGNLSTAAGIAIAT
HHHHHHCCCCCCEEEEECCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHCCEEEEE
NRTTRVLVGDLTGVHDIGALLIPLMEAKYRLQIVIGNDFGGGIFDTLEVHATPSDYKRVL
CCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCEEEEEECCCCCCCEEE
YVPCRVDFRLVSHAFGWKYTFCPDPNSLRKALKSRIFPHLLEVHLAV
EEECCCCHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TCASGIAFCLLNLLAERGVRDIVVAPGARSQALALAAVALERAGLVRLTVRIDERVAAF
CCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCEEEEEEEECHHHHHH
TAFGMAKALDSPAVVITTSGSAVANLYPAVVEASHSFVPLIVITADRPRELHGKRSNQTM
HHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCEEEEEEEECCCCHHHCCCCCCCCC
NQEKFFSHWVRFFCHIEQDVPGERVIDSALQAALGVSSLRGAGPGPVHINVAFDNPLSCA
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHCCCCCCCEEEEEEECCCCCHH
HAVQPGDNKGSALKHKKVLRSKRETLVLRKDDPPTVIIAGTGSGRRPADIACATGWPLVA
HCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCEEEEECCCCCCCCCCEEEECCCCEEE
EISSGSRFGPFLIKNYRNWLTENSLDIRRAIVFGHPTLSSEIPTFLTKRNIDTIVVAPFG
EECCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCHHHHHCCCCEEEEECCC
REFYNPSGTARPVANVLVDEDSFSGIPLGAAKFASASAGCISQDVISSDRPRGHRVTRQQ
HHHCCCCCCCCHHHHEEECCCCCCCCCCCHHHHCCCCCCCCHHHHHCCCCCCCCHHHHHH
LVSEIWNITKEDEILFFGSSQLIRVADSILPGKRVTVFANRGLSGIDGNLSTAAGIAIAT
HHHHHHCCCCCCEEEEECCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHCCEEEEE
NRTTRVLVGDLTGVHDIGALLIPLMEAKYRLQIVIGNDFGGGIFDTLEVHATPSDYKRVL
CCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCEEEEEECCCCCCCEEE
YVPCRVDFRLVSHAFGWKYTFCPDPNSLRKALKSRIFPHLLEVHLAV
EEECCCCHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA