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The map label for this gene is pccB [H]

Identifier: 28572200

GI number: 28572200

Start: 24099

End: 25676

Strand: Reverse

Name: pccB [H]

Synonym: TW025

Alternate gene names: 28572200

Gene position: 25676-24099 (Counterclockwise)

Preceding gene: 28572202

Following gene: 28572196

Centisome position: 2.77

GC content: 46.64

Gene sequence:

>1578_bases
ATGACCGATAATTTTGACGAATCCAGTAGGGGGCTAGAGAAGTACAGGCAGGCATACGAAGTAGCAGTCACTTTGCGTGA
GGATTCTGCGAAGGAAAAACAACACTCAAAAGGGAAGATGACCGCCCGGGAGCGTATAGATAAACTGCTTGACGAAAACT
CGTTTACTGAAATAGACCAGTTTGCCATGCACAGCGCAAGTGGATTTGGTATCGAAAAAAACCACCCTTATGGCGACTCT
GTCGTCACAGGTTTTGGCTCAATAGACGGCAGGAAAGTTGCGGTATATTCTCAGGATTTCACGGTTTTCGGTGGGTCACT
CGGAGAAGTGGCCGGCAGGAAAATAGTAAAAACTATGGACCTGGCAATGTCTGCAGGAATACCCGTAATAGGTCTCCTTG
ATTCCGGTGGAGCCAGGATACAGGAGGGAATTGCGGCGCTTACTAAATACGGAGAAATATTCAAGCGAAATGTTGCGGCG
AGCGGAATTATTCCACAAATCTCAATAGTTATGGGGCCTGCGGCAGGTGGGGCATGCTACTCACCGGCACTTACAGATTT
TGTAATTATGGTGGACCAGATCAGCTATATGTTCATCACGGGACCTGATGTTATAAAAGCAGTTACAGGAGAAGAGGTGA
CTTTTGAGGATCTTGGTGGTGCCAATATGCATAACGCCACCTCTGGCGTCTCGCACTATATGGCTCATAGCGAAGATGAC
GCAATTGCATACGCCCGGGAGCTGATTGGGTTTCTGCCCAGTAATAACATGTCAGACCCTCCCACATGGCCACAGGATAT
TCATACGGAGGAGAGTGACCTTGAACTAAATTCAATTGTGCCCGAGAATCCAAATAAGCCTTATGATATGCAGTTTGTCA
TAGAGCGAATTGTTGATGATGGCAATTTTCTGGAAATTCAATCCGCATACGCAAAGAATGTCATCATCGGTTTTGCGCGA
ATATCAGGCTTTTCGATCGGTATTGTTGCGAATCAGCCTGCGCATATGGCAGGGGCTTTGGATATAAATGCAAGTGAGAA
AGCTGCACGGTTTGTTCGCTTTTGTGACGCTTTTTCAATTCCTATTCTGACACTGGTTGATGTGCCGGGATATTTACCCG
GGACAGAGCAGGAATGGACAGGTATTATCAGGCGCGGTGCGAAGCTTCTCTATGCTTATTCCGAGGCAACTGTACCGATG
GTGACAATTATCCTGCGTAAAGCGTATGGCGGGGCCTATATAGTAATGGGCTCAAAAGCGTTAGGTGCCGATATAAACCT
TGCATGGCCATCAGCAGAAATTGCCGTCATGGGCGCCCAGGGTGCTGTGAATATTCTCTATAGAAATGATATAAAGCGGC
TCGTGCAAGCCGGTGAAGACGCAAATCCAATGAAAGAGAAATTGGTCAAAGAGTACACCGATAAATTTGCCAATCCTTTT
ATTGCCGCCCAGAGAGGATATCTGGATGCGATTATAGAGCCAGCACAAACGCGAATTGCGATTGCTACAGCCTTTCAAAC
CATCAGAACGAAGCGTTACAAACAAATAGACAGAAAGCACGGAAGCATACCACTGTAA

Upstream 100 bases:

>100_bases
TGTTGATCCAACAGTGTCTAGAAATACACAAGGGAACATGAAAGAATATTAGAACAAAACAAATGCCAACAAAAAAATGA
ATCGAGGATAGAGTACCCTG

Downstream 100 bases:

>100_bases
TTGATGCCTGTTCGGTTGGACATAAGGGGCGCTTTGGTGCCCATCAAACCCTTGCGCCGTTAAGCAAGGGCAGAATGCGT
GTATTGCGAAATTTTATTAC

Product: propionyl-CoA carboxylase beta chain

Products: NA

Alternate protein names: PCCase; Propanoyl-CoA:carbon dioxide ligase [H]

Number of amino acids: Translated: 525; Mature: 524

Protein sequence:

>525_residues
MTDNFDESSRGLEKYRQAYEVAVTLREDSAKEKQHSKGKMTARERIDKLLDENSFTEIDQFAMHSASGFGIEKNHPYGDS
VVTGFGSIDGRKVAVYSQDFTVFGGSLGEVAGRKIVKTMDLAMSAGIPVIGLLDSGGARIQEGIAALTKYGEIFKRNVAA
SGIIPQISIVMGPAAGGACYSPALTDFVIMVDQISYMFITGPDVIKAVTGEEVTFEDLGGANMHNATSGVSHYMAHSEDD
AIAYARELIGFLPSNNMSDPPTWPQDIHTEESDLELNSIVPENPNKPYDMQFVIERIVDDGNFLEIQSAYAKNVIIGFAR
ISGFSIGIVANQPAHMAGALDINASEKAARFVRFCDAFSIPILTLVDVPGYLPGTEQEWTGIIRRGAKLLYAYSEATVPM
VTIILRKAYGGAYIVMGSKALGADINLAWPSAEIAVMGAQGAVNILYRNDIKRLVQAGEDANPMKEKLVKEYTDKFANPF
IAAQRGYLDAIIEPAQTRIAIATAFQTIRTKRYKQIDRKHGSIPL

Sequences:

>Translated_525_residues
MTDNFDESSRGLEKYRQAYEVAVTLREDSAKEKQHSKGKMTARERIDKLLDENSFTEIDQFAMHSASGFGIEKNHPYGDS
VVTGFGSIDGRKVAVYSQDFTVFGGSLGEVAGRKIVKTMDLAMSAGIPVIGLLDSGGARIQEGIAALTKYGEIFKRNVAA
SGIIPQISIVMGPAAGGACYSPALTDFVIMVDQISYMFITGPDVIKAVTGEEVTFEDLGGANMHNATSGVSHYMAHSEDD
AIAYARELIGFLPSNNMSDPPTWPQDIHTEESDLELNSIVPENPNKPYDMQFVIERIVDDGNFLEIQSAYAKNVIIGFAR
ISGFSIGIVANQPAHMAGALDINASEKAARFVRFCDAFSIPILTLVDVPGYLPGTEQEWTGIIRRGAKLLYAYSEATVPM
VTIILRKAYGGAYIVMGSKALGADINLAWPSAEIAVMGAQGAVNILYRNDIKRLVQAGEDANPMKEKLVKEYTDKFANPF
IAAQRGYLDAIIEPAQTRIAIATAFQTIRTKRYKQIDRKHGSIPL
>Mature_524_residues
TDNFDESSRGLEKYRQAYEVAVTLREDSAKEKQHSKGKMTARERIDKLLDENSFTEIDQFAMHSASGFGIEKNHPYGDSV
VTGFGSIDGRKVAVYSQDFTVFGGSLGEVAGRKIVKTMDLAMSAGIPVIGLLDSGGARIQEGIAALTKYGEIFKRNVAAS
GIIPQISIVMGPAAGGACYSPALTDFVIMVDQISYMFITGPDVIKAVTGEEVTFEDLGGANMHNATSGVSHYMAHSEDDA
IAYARELIGFLPSNNMSDPPTWPQDIHTEESDLELNSIVPENPNKPYDMQFVIERIVDDGNFLEIQSAYAKNVIIGFARI
SGFSIGIVANQPAHMAGALDINASEKAARFVRFCDAFSIPILTLVDVPGYLPGTEQEWTGIIRRGAKLLYAYSEATVPMV
TIILRKAYGGAYIVMGSKALGADINLAWPSAEIAVMGAQGAVNILYRNDIKRLVQAGEDANPMKEKLVKEYTDKFANPFI
AAQRGYLDAIIEPAQTRIAIATAFQTIRTKRYKQIDRKHGSIPL

Specific function: This Protein Is A Component Of The Acetyl Coenzyme A Carboxylase Complex; First, Biotin Carboxylase Catalyzes The Carboxylation Of The Carrier Protein And Then The Transcarboxylase Transfers The Carboxyl Group To Form Malonyl-CoA. [C]

COG id: COG4799

COG function: function code I; Acetyl-CoA carboxylase, carboxyltransferase component (subunits alpha and beta)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 carboxyltransferase domain [H]

Homologues:

Organism=Homo sapiens, GI119943100, Length=498, Percent_Identity=54.6184738955823, Blast_Score=533, Evalue=1e-151,
Organism=Homo sapiens, GI295821216, Length=518, Percent_Identity=52.5096525096525, Blast_Score=520, Evalue=1e-147,
Organism=Homo sapiens, GI11545863, Length=514, Percent_Identity=33.2684824902724, Blast_Score=249, Evalue=5e-66,
Organism=Homo sapiens, GI134142062, Length=370, Percent_Identity=23.2432432432432, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI1788655, Length=191, Percent_Identity=27.2251308900524, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25147362, Length=520, Percent_Identity=54.6153846153846, Blast_Score=561, Evalue=1e-160,
Organism=Caenorhabditis elegans, GI25147359, Length=520, Percent_Identity=54.6153846153846, Blast_Score=561, Evalue=1e-160,
Organism=Caenorhabditis elegans, GI17552936, Length=530, Percent_Identity=32.6415094339623, Blast_Score=268, Evalue=6e-72,
Organism=Drosophila melanogaster, GI24586065, Length=498, Percent_Identity=33.5341365461847, Blast_Score=254, Evalue=1e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000438
- InterPro:   IPR000022
- InterPro:   IPR011763
- InterPro:   IPR011762 [H]

Pfam domain/function: PF01039 Carboxyl_trans [H]

EC number: =6.4.1.3 [H]

Molecular weight: Translated: 57139; Mature: 57008

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS50980 COA_CT_NTER ; PS50989 COA_CT_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDNFDESSRGLEKYRQAYEVAVTLREDSAKEKQHSKGKMTARERIDKLLDENSFTEIDQ
CCCCCCCHHHHHHHHHHHHHEEEEEECCCHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHH
FAMHSASGFGIEKNHPYGDSVVTGFGSIDGRKVAVYSQDFTVFGGSLGEVAGRKIVKTMD
HHHHCCCCCCCCCCCCCCCHHEECCCCCCCCEEEEEECCCEEECCCHHHHHHHHHHHHHH
LAMSAGIPVIGLLDSGGARIQEGIAALTKYGEIFKRNVAASGIIPQISIVMGPAAGGACY
HHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCC
SPALTDFVIMVDQISYMFITGPDVIKAVTGEEVTFEDLGGANMHNATSGVSHYMAHSEDD
CHHHHHHHHHHHCCCEEEEECHHHHHHHCCCCEEHHHCCCCCCCCHHHHHHHHHHCCCCH
AIAYARELIGFLPSNNMSDPPTWPQDIHTEESDLELNSIVPENPNKPYDMQFVIERIVDD
HHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHCC
GNFLEIQSAYAKNVIIGFARISGFSIGIVANQPAHMAGALDINASEKAARFVRFCDAFSI
CCEEEEHHHHHCHHEEEEEECCCEEEEEEECCCCHHEEEEECCCHHHHHHHHHHHHHHCC
PILTLVDVPGYLPGTEQEWTGIIRRGAKLLYAYSEATVPMVTIILRKAYGGAYIVMGSKA
CEEEEECCCCCCCCCCHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCEEEEECCCC
LGADINLAWPSAEIAVMGAQGAVNILYRNDIKRLVQAGEDANPMKEKLVKEYTDKFANPF
CCCCEEEECCCCEEEEEECCCCEEEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCH
IAAQRGYLDAIIEPAQTRIAIATAFQTIRTKRYKQIDRKHGSIPL
HHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TDNFDESSRGLEKYRQAYEVAVTLREDSAKEKQHSKGKMTARERIDKLLDENSFTEIDQ
CCCCCCHHHHHHHHHHHHHEEEEEECCCHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHH
FAMHSASGFGIEKNHPYGDSVVTGFGSIDGRKVAVYSQDFTVFGGSLGEVAGRKIVKTMD
HHHHCCCCCCCCCCCCCCCHHEECCCCCCCCEEEEEECCCEEECCCHHHHHHHHHHHHHH
LAMSAGIPVIGLLDSGGARIQEGIAALTKYGEIFKRNVAASGIIPQISIVMGPAAGGACY
HHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCC
SPALTDFVIMVDQISYMFITGPDVIKAVTGEEVTFEDLGGANMHNATSGVSHYMAHSEDD
CHHHHHHHHHHHCCCEEEEECHHHHHHHCCCCEEHHHCCCCCCCCHHHHHHHHHHCCCCH
AIAYARELIGFLPSNNMSDPPTWPQDIHTEESDLELNSIVPENPNKPYDMQFVIERIVDD
HHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHCC
GNFLEIQSAYAKNVIIGFARISGFSIGIVANQPAHMAGALDINASEKAARFVRFCDAFSI
CCEEEEHHHHHCHHEEEEEECCCEEEEEEECCCCHHEEEEECCCHHHHHHHHHHHHHHCC
PILTLVDVPGYLPGTEQEWTGIIRRGAKLLYAYSEATVPMVTIILRKAYGGAYIVMGSKA
CEEEEECCCCCCCCCCHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCEEEEECCCC
LGADINLAWPSAEIAVMGAQGAVNILYRNDIKRLVQAGEDANPMKEKLVKEYTDKFANPF
CCCCEEEECCCCEEEEEECCCCEEEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCH
IAAQRGYLDAIIEPAQTRIAIATAFQTIRTKRYKQIDRKHGSIPL
HHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8830278 [H]