| Definition | Tropheryma whipplei TW08/27, complete genome. |
|---|---|
| Accession | NC_004551 |
| Length | 925,938 |
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The map label for this gene is dkgA [H]
Identifier: 28572191
GI number: 28572191
Start: 15432
End: 16280
Strand: Direct
Name: dkgA [H]
Synonym: TW016
Alternate gene names: 28572191
Gene position: 15432-16280 (Clockwise)
Preceding gene: 28572190
Following gene: 28572194
Centisome position: 1.67
GC content: 48.53
Gene sequence:
>849_bases GTGGTGTTTGTATTGACTTATGTTCCTAGCGTTCTCTTGGGTGACGGTGTATCGATTCCCCAGTTTGGATTGGGTACCTA TGAGCTTCCTCCCAACGAGACATCTTCGGTGGTGCAGTCTGCGATCGAGTTGGGCTATCGCCATATCGATACGGCTTCAC TCTACGCTAATGAGCGAGAGGTTGGCCGCGCTATTGCTAGCAGCGGAATAAAGCGTGAGGAGTTTTTTGTCACAACAAAG CTATGGAATTCTGATCAACCTAAGCCGAGAGAGGCGTTCGAGCGCAGCCTTGACCTCCTCTCACTTGATTATGTTGATTT GTATCTTATCCATTGGCCTTGCCCGCCGAGTGAGCTGTATATAAATGTCTGGGAAGTGCTATTGGAGCTCAAGGAGTCGG GCCGTGCCAAGTCTGTTGGTGTGTCAAATTTTTTGTCCGAGCATATCAATAAAATAAAAGAAGCGGGCTTTCCGCTGCCC TCTGTAAACCAAATAGAACTTCATCCGTGGCTTCAACAGCGTGAACTTGTCTTGTCTTGCAGAAATTATGGCATACAAAT CGAGTCCTGGGGACCTTTCGCGCGTTTTTCTAAGGATCTTGATGCGTTCCCGGAACTGACACGCCCAGCAAAAGAATATG GAAAAACCCCGGCTCAGGTCATTTTGCGTTGGCATATTCAGAAGGGGTATGTTGTCTTCCCAAAGACAGTGCATGCGGAA CGGTTGCGGCAGAATATTGATATATTCGATTTTGACTTGACTCCTGACGAAATATCTGGGATTGATAGCCTTGATGCGGG CTTTAGGACCGGTCCGGATCCGAGCAGCTTTCGAGCCGGCCTCGATTGA
Upstream 100 bases:
>100_bases AGAGGTTGAATATGCGCACAGTACAACTTACACGATCACGGTTGTTGAGGGCACTGCGCCTGGGGCAAACTGAGTTTGTT GATGTATTTAGTTCGTATTG
Downstream 100 bases:
>100_bases TCTAACCACCCAATAGTCCCAGTGATCTTTTCCAGCGGTATAAGTAATTCCCGCCGTTTGGTTTTGTCAAAAATATGTTT CAGGGACTACTGTCATGTAC
Product: 2,5-diketo-D-gluconic acid reductase A
Products: NA
Alternate protein names: 2,5-DKG reductase A; 2,5-DKGR A; 25DKGR-A; AKR5C [H]
Number of amino acids: Translated: 282; Mature: 282
Protein sequence:
>282_residues MVFVLTYVPSVLLGDGVSIPQFGLGTYELPPNETSSVVQSAIELGYRHIDTASLYANEREVGRAIASSGIKREEFFVTTK LWNSDQPKPREAFERSLDLLSLDYVDLYLIHWPCPPSELYINVWEVLLELKESGRAKSVGVSNFLSEHINKIKEAGFPLP SVNQIELHPWLQQRELVLSCRNYGIQIESWGPFARFSKDLDAFPELTRPAKEYGKTPAQVILRWHIQKGYVVFPKTVHAE RLRQNIDIFDFDLTPDEISGIDSLDAGFRTGPDPSSFRAGLD
Sequences:
>Translated_282_residues MVFVLTYVPSVLLGDGVSIPQFGLGTYELPPNETSSVVQSAIELGYRHIDTASLYANEREVGRAIASSGIKREEFFVTTK LWNSDQPKPREAFERSLDLLSLDYVDLYLIHWPCPPSELYINVWEVLLELKESGRAKSVGVSNFLSEHINKIKEAGFPLP SVNQIELHPWLQQRELVLSCRNYGIQIESWGPFARFSKDLDAFPELTRPAKEYGKTPAQVILRWHIQKGYVVFPKTVHAE RLRQNIDIFDFDLTPDEISGIDSLDAGFRTGPDPSSFRAGLD >Mature_282_residues MVFVLTYVPSVLLGDGVSIPQFGLGTYELPPNETSSVVQSAIELGYRHIDTASLYANEREVGRAIASSGIKREEFFVTTK LWNSDQPKPREAFERSLDLLSLDYVDLYLIHWPCPPSELYINVWEVLLELKESGRAKSVGVSNFLSEHINKIKEAGFPLP SVNQIELHPWLQQRELVLSCRNYGIQIESWGPFARFSKDLDAFPELTRPAKEYGKTPAQVILRWHIQKGYVVFPKTVHAE RLRQNIDIFDFDLTPDEISGIDSLDAGFRTGPDPSSFRAGLD
Specific function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG). 5-keto-D-fructose and dihydroxyacetone can also serve as substrates. 25DKGR-A exhibits a greater selectivity for the substrate and higher thermal stability than
COG id: COG0656
COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the aldo/keto reductase family [H]
Homologues:
Organism=Homo sapiens, GI24497577, Length=293, Percent_Identity=35.4948805460751, Blast_Score=194, Evalue=1e-49, Organism=Homo sapiens, GI5174391, Length=293, Percent_Identity=35.4948805460751, Blast_Score=194, Evalue=1e-49, Organism=Homo sapiens, GI5453543, Length=298, Percent_Identity=38.255033557047, Blast_Score=184, Evalue=9e-47, Organism=Homo sapiens, GI310109920, Length=298, Percent_Identity=38.255033557047, Blast_Score=183, Evalue=2e-46, Organism=Homo sapiens, GI310109922, Length=280, Percent_Identity=39.2857142857143, Blast_Score=182, Evalue=3e-46, Organism=Homo sapiens, GI45446745, Length=298, Percent_Identity=37.9194630872483, Blast_Score=181, Evalue=6e-46, Organism=Homo sapiens, GI4503285, Length=298, Percent_Identity=37.9194630872483, Blast_Score=181, Evalue=6e-46, Organism=Homo sapiens, GI223468663, Length=294, Percent_Identity=35.3741496598639, Blast_Score=180, Evalue=1e-45, Organism=Homo sapiens, GI93277124, Length=309, Percent_Identity=33.3333333333333, Blast_Score=176, Evalue=2e-44, Organism=Homo sapiens, GI5174695, Length=296, Percent_Identity=37.1621621621622, Blast_Score=173, Evalue=1e-43, Organism=Homo sapiens, GI24497585, Length=297, Percent_Identity=37.037037037037, Blast_Score=172, Evalue=4e-43, Organism=Homo sapiens, GI24497583, Length=294, Percent_Identity=37.0748299319728, Blast_Score=171, Evalue=6e-43, Organism=Homo sapiens, GI4502049, Length=294, Percent_Identity=35.0340136054422, Blast_Score=159, Evalue=3e-39, Organism=Homo sapiens, GI300116273, Length=275, Percent_Identity=36.3636363636364, Blast_Score=154, Evalue=9e-38, Organism=Homo sapiens, GI291291012, Length=272, Percent_Identity=32.7205882352941, Blast_Score=153, Evalue=1e-37, Organism=Homo sapiens, GI300116271, Length=279, Percent_Identity=35.4838709677419, Blast_Score=146, Evalue=2e-35, Organism=Homo sapiens, GI310109926, Length=117, Percent_Identity=50.4273504273504, Blast_Score=101, Evalue=9e-22, Organism=Homo sapiens, GI207028673, Length=117, Percent_Identity=49.5726495726496, Blast_Score=100, Evalue=3e-21, Organism=Homo sapiens, GI310109924, Length=152, Percent_Identity=33.5526315789474, Blast_Score=94, Evalue=1e-19, Organism=Escherichia coli, GI87082198, Length=270, Percent_Identity=45.5555555555556, Blast_Score=261, Evalue=4e-71, Organism=Escherichia coli, GI1786400, Length=257, Percent_Identity=39.6887159533074, Blast_Score=178, Evalue=3e-46, Organism=Escherichia coli, GI1788081, Length=279, Percent_Identity=27.9569892473118, Blast_Score=92, Evalue=3e-20, Organism=Escherichia coli, GI87081735, Length=302, Percent_Identity=24.8344370860927, Blast_Score=69, Evalue=4e-13, Organism=Caenorhabditis elegans, GI17537077, Length=295, Percent_Identity=36.6101694915254, Blast_Score=186, Evalue=1e-47, Organism=Caenorhabditis elegans, GI17537075, Length=301, Percent_Identity=36.5448504983389, Blast_Score=184, Evalue=4e-47, Organism=Caenorhabditis elegans, GI71998625, Length=277, Percent_Identity=34.2960288808664, Blast_Score=172, Evalue=2e-43, Organism=Caenorhabditis elegans, GI17566692, Length=289, Percent_Identity=33.5640138408304, Blast_Score=157, Evalue=6e-39, Organism=Caenorhabditis elegans, GI17550248, Length=287, Percent_Identity=33.1010452961672, Blast_Score=157, Evalue=8e-39, Organism=Caenorhabditis elegans, GI17537079, Length=298, Percent_Identity=31.5436241610738, Blast_Score=156, Evalue=9e-39, Organism=Caenorhabditis elegans, GI17561300, Length=252, Percent_Identity=34.5238095238095, Blast_Score=152, Evalue=3e-37, Organism=Caenorhabditis elegans, GI17564128, Length=300, Percent_Identity=32.3333333333333, Blast_Score=150, Evalue=9e-37, Organism=Caenorhabditis elegans, GI17561298, Length=272, Percent_Identity=31.6176470588235, Blast_Score=147, Evalue=8e-36, Organism=Caenorhabditis elegans, GI17552492, Length=274, Percent_Identity=31.021897810219, Blast_Score=145, Evalue=2e-35, Organism=Caenorhabditis elegans, GI17538386, Length=270, Percent_Identity=31.4814814814815, Blast_Score=137, Evalue=5e-33, Organism=Caenorhabditis elegans, GI17562292, Length=275, Percent_Identity=30.1818181818182, Blast_Score=122, Evalue=2e-28, Organism=Saccharomyces cerevisiae, GI6320576, Length=280, Percent_Identity=40.7142857142857, Blast_Score=208, Evalue=9e-55, Organism=Saccharomyces cerevisiae, GI6324694, Length=283, Percent_Identity=39.2226148409894, Blast_Score=203, Evalue=3e-53, Organism=Saccharomyces cerevisiae, GI6322556, Length=267, Percent_Identity=37.8277153558052, Blast_Score=188, Evalue=8e-49, Organism=Saccharomyces cerevisiae, GI6321896, Length=309, Percent_Identity=32.6860841423948, Blast_Score=140, Evalue=2e-34, Organism=Saccharomyces cerevisiae, GI6319625, Length=288, Percent_Identity=30.5555555555556, Blast_Score=128, Evalue=1e-30, Organism=Saccharomyces cerevisiae, GI6320079, Length=280, Percent_Identity=31.7857142857143, Blast_Score=117, Evalue=2e-27, Organism=Drosophila melanogaster, GI24644950, Length=297, Percent_Identity=35.6902356902357, Blast_Score=187, Evalue=8e-48, Organism=Drosophila melanogaster, GI24663317, Length=299, Percent_Identity=34.7826086956522, Blast_Score=184, Evalue=7e-47, Organism=Drosophila melanogaster, GI221378297, Length=317, Percent_Identity=34.0694006309148, Blast_Score=182, Evalue=2e-46, Organism=Drosophila melanogaster, GI24662789, Length=298, Percent_Identity=33.5570469798658, Blast_Score=177, Evalue=9e-45, Organism=Drosophila melanogaster, GI45553081, Length=296, Percent_Identity=35.1351351351351, Blast_Score=175, Evalue=3e-44, Organism=Drosophila melanogaster, GI24657054, Length=296, Percent_Identity=35.472972972973, Blast_Score=174, Evalue=5e-44, Organism=Drosophila melanogaster, GI20129731, Length=295, Percent_Identity=35.5932203389831, Blast_Score=172, Evalue=2e-43, Organism=Drosophila melanogaster, GI21356425, Length=304, Percent_Identity=35.1973684210526, Blast_Score=169, Evalue=2e-42, Organism=Drosophila melanogaster, GI24662785, Length=297, Percent_Identity=36.3636363636364, Blast_Score=159, Evalue=1e-39, Organism=Drosophila melanogaster, GI24662781, Length=298, Percent_Identity=35.9060402684564, Blast_Score=157, Evalue=5e-39, Organism=Drosophila melanogaster, GI281366140, Length=257, Percent_Identity=35.0194552529183, Blast_Score=146, Evalue=1e-35,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR001395 - InterPro: IPR018170 - InterPro: IPR020471 - InterPro: IPR023210 [H]
Pfam domain/function: PF00248 Aldo_ket_red [H]
EC number: =1.1.1.274 [H]
Molecular weight: Translated: 31928; Mature: 31928
Theoretical pI: Translated: 4.94; Mature: 4.94
Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.7 %Cys (Translated Protein) 0.4 %Met (Translated Protein) 1.1 %Cys+Met (Translated Protein) 0.7 %Cys (Mature Protein) 0.4 %Met (Mature Protein) 1.1 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MVFVLTYVPSVLLGDGVSIPQFGLGTYELPPNETSSVVQSAIELGYRHIDTASLYANERE CEEEEEHHHHHHHCCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHCCHHHHHCCHHH VGRAIASSGIKREEFFVTTKLWNSDQPKPREAFERSLDLLSLDYVDLYLIHWPCPPSELY HHHHHHHCCCCCHHEEEEEEECCCCCCCHHHHHHHHHHHHEECCEEEEEEECCCCCHHHH INVWEVLLELKESGRAKSVGVSNFLSEHINKIKEAGFPLPSVNQIELHPWLQQRELVLSC HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHH RNYGIQIESWGPFARFSKDLDAFPELTRPAKEYGKTPAQVILRWHIQKGYVVFPKTVHAE HHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHEEECCCEEECCCHHHHH RLRQNIDIFDFDLTPDEISGIDSLDAGFRTGPDPSSFRAGLD HHHCCCCEEEECCCHHHHCCCCHHCCCCCCCCCHHHHCCCCC >Mature Secondary Structure MVFVLTYVPSVLLGDGVSIPQFGLGTYELPPNETSSVVQSAIELGYRHIDTASLYANERE CEEEEEHHHHHHHCCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHCCHHHHHCCHHH VGRAIASSGIKREEFFVTTKLWNSDQPKPREAFERSLDLLSLDYVDLYLIHWPCPPSELY HHHHHHHCCCCCHHEEEEEEECCCCCCCHHHHHHHHHHHHEECCEEEEEEECCCCCHHHH INVWEVLLELKESGRAKSVGVSNFLSEHINKIKEAGFPLPSVNQIELHPWLQQRELVLSC HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHH RNYGIQIESWGPFARFSKDLDAFPELTRPAKEYGKTPAQVILRWHIQKGYVVFPKTVHAE HHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHEEECCCEEECCCHHHHH RLRQNIDIFDFDLTPDEISGIDSLDAGFRTGPDPSSFRAGLD HHHCCCCEEEECCCHHHHCCCCHHCCCCCCCCCHHHHCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 3597405; 10737928; 9618487; 11399090 [H]