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The map label for this gene is thiN [H]

Identifier: 28572183

GI number: 28572183

Start: 9640

End: 10332

Strand: Reverse

Name: thiN [H]

Synonym: TW008

Alternate gene names: 28572183

Gene position: 10332-9640 (Counterclockwise)

Preceding gene: 28572185

Following gene: NA

Centisome position: 1.12

GC content: 49.21

Gene sequence:

>693_bases
GTGCTTGGAATGGGTGTTTGCTACATATATGGTGCCGGTGAGCACTTTACACCCCCCGAAGAAGTGGGGATGCACGACTT
GGTTATCGCGGCTGATGGCGGCTTGATGCAGCTCAAGAAGCATGGTGCGCGCATTGATCTCTATGTCGGCGACCTTGATT
CAATTGAGTCTATTCCAATAAACATTGATCGGGTGATTTTGCCCAGAGAAAAAGATAAAACTGACATGATGCAGGCAGCC
CTGATTGGTGCCGAGCGGGGCTTTAGAACTTTTTGCATATTTGGGGGTACCGGTGGGCGGCTTGACCATACCATTGGAAA
TATCCAGCTACTGGCCGAACTGACAAATATGGGATATAGCGTGTTTATTGTCGGGAAGTATGATGTGGTGACAGCGATCC
GAAACAATGAGATTTACTTTCCGTCGCATTTCACCGGCAATATCTCAATCTTCCCGCACTCCGGCGATGCCAGCGGGGTT
TATGAGGAGGGGCTAAAATATGCCCTGAACAACGCACATATACCGGGTACCTTTAATGGTGTTAGCAATGAGTTTATAGG
GGTGAAGAGCAGGGTTGCGGTCGAGTCTGGCATGCTTCTTGTGGTGTACGAGCGCCAAGGCGGCAGAATGCATTTGAACA
TTTCGACGTGTGCGATGACGGCAGGAAATCAAATACAAAAACCAAGCGGTTGA

Upstream 100 bases:

>100_bases
CAAGGGGGCACAAAAGGAGCGAGCCATACAAAAGCCACCCGGACCGTACAATTAGCTTGTAACACAATTATATCCGCGGT
CGTCACCGATATTGAAGGTT

Downstream 100 bases:

>100_bases
GATGTCATATCCAACATTATGGGCGATCCGATTCCAGTTCTACAGACCCGACTGCACGGCCCGACTGTATGCCAGACCTG
ACTGCATGGCTAGCCAGTTA

Product: hypothetical protein

Products: NA

Alternate protein names: TPK; Thiamine diphosphokinase [H]

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MLGMGVCYIYGAGEHFTPPEEVGMHDLVIAADGGLMQLKKHGARIDLYVGDLDSIESIPINIDRVILPREKDKTDMMQAA
LIGAERGFRTFCIFGGTGGRLDHTIGNIQLLAELTNMGYSVFIVGKYDVVTAIRNNEIYFPSHFTGNISIFPHSGDASGV
YEEGLKYALNNAHIPGTFNGVSNEFIGVKSRVAVESGMLLVVYERQGGRMHLNISTCAMTAGNQIQKPSG

Sequences:

>Translated_230_residues
MLGMGVCYIYGAGEHFTPPEEVGMHDLVIAADGGLMQLKKHGARIDLYVGDLDSIESIPINIDRVILPREKDKTDMMQAA
LIGAERGFRTFCIFGGTGGRLDHTIGNIQLLAELTNMGYSVFIVGKYDVVTAIRNNEIYFPSHFTGNISIFPHSGDASGV
YEEGLKYALNNAHIPGTFNGVSNEFIGVKSRVAVESGMLLVVYERQGGRMHLNISTCAMTAGNQIQKPSG
>Mature_230_residues
MLGMGVCYIYGAGEHFTPPEEVGMHDLVIAADGGLMQLKKHGARIDLYVGDLDSIESIPINIDRVILPREKDKTDMMQAA
LIGAERGFRTFCIFGGTGGRLDHTIGNIQLLAELTNMGYSVFIVGKYDVVTAIRNNEIYFPSHFTGNISIFPHSGDASGV
YEEGLKYALNNAHIPGTFNGVSNEFIGVKSRVAVESGMLLVVYERQGGRMHLNISTCAMTAGNQIQKPSG

Specific function: Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate [H]

COG id: COG1564

COG function: function code H; Thiamine pyrophosphokinase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiamine pyrophosphokinase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006282
- InterPro:   IPR007373
- InterPro:   IPR007371 [H]

Pfam domain/function: PF04263 TPK_catalytic [H]

EC number: =2.7.6.2 [H]

Molecular weight: Translated: 24945; Mature: 24945

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGMGVCYIYGAGEHFTPPEEVGMHDLVIAADGGLMQLKKHGARIDLYVGDLDSIESIPI
CCCCEEEEEEECCCCCCCHHHCCCEEEEEEECCCEEEHHHCCCEEEEEECCCCCCCCCCC
NIDRVILPREKDKTDMMQAALIGAERGFRTFCIFGGTGGRLDHTIGNIQLLAELTNMGYS
EEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCEEEEHHHCCEE
VFIVGKYDVVTAIRNNEIYFPSHFTGNISIFPHSGDASGVYEEGLKYALNNAHIPGTFNG
EEEEECEEEEEEEECCEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCC
VSNEFIGVKSRVAVESGMLLVVYERQGGRMHLNISTCAMTAGNQIQKPSG
CCCCEECCCEEEEECCCCEEEEEECCCCEEEEEEEHEEEECCCCCCCCCC
>Mature Secondary Structure
MLGMGVCYIYGAGEHFTPPEEVGMHDLVIAADGGLMQLKKHGARIDLYVGDLDSIESIPI
CCCCEEEEEEECCCCCCCHHHCCCEEEEEEECCCEEEHHHCCCEEEEEECCCCCCCCCCC
NIDRVILPREKDKTDMMQAALIGAERGFRTFCIFGGTGGRLDHTIGNIQLLAELTNMGYS
EEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCEEEEHHHCCEE
VFIVGKYDVVTAIRNNEIYFPSHFTGNISIFPHSGDASGVYEEGLKYALNNAHIPGTFNG
EEEEECEEEEEEEECCEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCC
VSNEFIGVKSRVAVESGMLLVVYERQGGRMHLNISTCAMTAGNQIQKPSG
CCCCEECCCEEEEECCCCEEEEEECCCCEEEEEEEHEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377 [H]