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The map label for this gene is folE [H]

Identifier: 284159980

GI number: 284159980

Start: 53360

End: 54070

Strand: Reverse

Name: folE [H]

Synonym: BURPS668_A0064

Alternate gene names: 284159980

Gene position: 54070-53360 (Counterclockwise)

Preceding gene: 126442565

Following gene: 126444053

Centisome position: 1.73

GC content: 67.51

Gene sequence:

>711_bases
ATGACCGCGGGCAAGGCGGCGGCACCGGCCGCGCACCAGGCGGCGCGCAGGACATCGAACAAAGCGAGCAAGACGGCGAG
CTCGGCGGCCACGCACGAGCCGGCCGGCGCGCCCGCGAACCGGCCGAGCCGCGACGAAGCCGAAGCGGCCGTTCGCGTGC
TGCTGCGGTGGGCCGGCGACGATCCGCAACGCGAGGGGCTCGTCGATACGCCGGCGCGCGTCGTGCGCGCCTACGAGGAG
TTCTTCGCCGGCTACGCGCTCGAACCGCGCGACATCCTCGCGCGCACGTTCAGCGAGGTCGACGGCTACGACGAAATGAT
CGTGCTGAAGGACATCCGCTTCGAAAGCTATTGCGAGCACCATATGGTGCCGATCATCGGCCGCGCGCACGTCGCATATC
TGCCGAACCATCGCGTCGTGGGCATCTCGAAGCTCGCGCGCCTCGTCGATGCGTTCGCGAAGCGCCTGCAGATCCAGGAA
AAGATGACGGTGCAGATCGCCGATACGCTGTTCGACGTGCTGCAGCCGAAGGGCGTCGGCGTGATTCTCGAAGCGGCGCA
CCAGTGCATCTCGACGCGCGGCATCCACAAGCCCGGCGTCGAGATGGTGACGTCGCGGATGCTCGGCACGTTCCGCACCG
ATCCGGCCACGCGCCGCGAATTCCTGTCGATCGTCGCGAATCCTTCCTCGGTCAACCTGACGAACACCTAA

Upstream 100 bases:

>100_bases
AGCGAGTCACGGCGCACGCTCGCGACGAAAGCATGAAACAATGATCGGCCGGCCGCAGCCTTCGCGGCCTTCATGGAGTT
CAATGCATATGGCTAAAGGA

Downstream 100 bases:

>100_bases
ATGCAGTCGAGCGCGAAACCAGCCACCTTACCCGTCGTGCTCGTGACGGGCGCCGCGCGCCGCGCGGGCCGCGCGTTCGC
GTTGCGCTTCGCCGCGCGCG

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I [H]

Number of amino acids: Translated: 236; Mature: 235

Protein sequence:

>236_residues
MTAGKAAAPAAHQAARRTSNKASKTASSAATHEPAGAPANRPSRDEAEAAVRVLLRWAGDDPQREGLVDTPARVVRAYEE
FFAGYALEPRDILARTFSEVDGYDEMIVLKDIRFESYCEHHMVPIIGRAHVAYLPNHRVVGISKLARLVDAFAKRLQIQE
KMTVQIADTLFDVLQPKGVGVILEAAHQCISTRGIHKPGVEMVTSRMLGTFRTDPATRREFLSIVANPSSVNLTNT

Sequences:

>Translated_236_residues
MTAGKAAAPAAHQAARRTSNKASKTASSAATHEPAGAPANRPSRDEAEAAVRVLLRWAGDDPQREGLVDTPARVVRAYEE
FFAGYALEPRDILARTFSEVDGYDEMIVLKDIRFESYCEHHMVPIIGRAHVAYLPNHRVVGISKLARLVDAFAKRLQIQE
KMTVQIADTLFDVLQPKGVGVILEAAHQCISTRGIHKPGVEMVTSRMLGTFRTDPATRREFLSIVANPSSVNLTNT
>Mature_235_residues
TAGKAAAPAAHQAARRTSNKASKTASSAATHEPAGAPANRPSRDEAEAAVRVLLRWAGDDPQREGLVDTPARVVRAYEEF
FAGYALEPRDILARTFSEVDGYDEMIVLKDIRFESYCEHHMVPIIGRAHVAYLPNHRVVGISKLARLVDAFAKRLQIQEK
MTVQIADTLFDVLQPKGVGVILEAAHQCISTRGIHKPGVEMVTSRMLGTFRTDPATRREFLSIVANPSSVNLTNT

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family [H]

Homologues:

Organism=Homo sapiens, GI66932968, Length=209, Percent_Identity=42.1052631578947, Blast_Score=182, Evalue=3e-46,
Organism=Homo sapiens, GI4503949, Length=209, Percent_Identity=42.1052631578947, Blast_Score=182, Evalue=3e-46,
Organism=Homo sapiens, GI66932970, Length=179, Percent_Identity=38.5474860335196, Blast_Score=138, Evalue=3e-33,
Organism=Homo sapiens, GI66932972, Length=169, Percent_Identity=40.2366863905325, Blast_Score=136, Evalue=1e-32,
Organism=Escherichia coli, GI1788476, Length=175, Percent_Identity=37.7142857142857, Blast_Score=115, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17560486, Length=176, Percent_Identity=43.75, Blast_Score=176, Evalue=7e-45,
Organism=Saccharomyces cerevisiae, GI6321706, Length=181, Percent_Identity=45.3038674033149, Blast_Score=175, Evalue=4e-45,
Organism=Drosophila melanogaster, GI24656782, Length=176, Percent_Identity=45.4545454545455, Blast_Score=162, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24656791, Length=176, Percent_Identity=45.4545454545455, Blast_Score=161, Evalue=4e-40,
Organism=Drosophila melanogaster, GI24656796, Length=176, Percent_Identity=45.4545454545455, Blast_Score=161, Evalue=4e-40,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =3.5.4.16 [H]

Molecular weight: Translated: 25842; Mature: 25711

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1 ; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAGKAAAPAAHQAARRTSNKASKTASSAATHEPAGAPANRPSRDEAEAAVRVLLRWAGD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
DPQREGLVDTPARVVRAYEEFFAGYALEPRDILARTFSEVDGYDEMIVLKDIRFESYCEH
CHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHH
HMVPIIGRAHVAYLPNHRVVGISKLARLVDAFAKRLQIQEKMTVQIADTLFDVLQPKGVG
CCCCHHCCCEEEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
VILEAAHQCISTRGIHKPGVEMVTSRMLGTFRTDPATRREFLSIVANPSSVNLTNT
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TAGKAAAPAAHQAARRTSNKASKTASSAATHEPAGAPANRPSRDEAEAAVRVLLRWAGD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
DPQREGLVDTPARVVRAYEEFFAGYALEPRDILARTFSEVDGYDEMIVLKDIRFESYCEH
CHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHH
HMVPIIGRAHVAYLPNHRVVGISKLARLVDAFAKRLQIQEKMTVQIADTLFDVLQPKGVG
CCCCHHCCCEEEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
VILEAAHQCISTRGIHKPGVEMVTSRMLGTFRTDPATRREFLSIVANPSSVNLTNT
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]