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The map label for this gene is amd [H]

Identifier: 28379468

GI number: 28379468

Start: 2706304

End: 2707503

Strand: Direct

Name: amd [H]

Synonym: lp_3044

Alternate gene names: 28379468

Gene position: 2706304-2707503 (Clockwise)

Preceding gene: 28379467

Following gene: 28379473

Centisome position: 81.8

GC content: 52.08

Gene sequence:

>1200_bases
ATGACCCTTCAAGCACAACTCATGCAACAACTCACGGCGGCGGAACCGGAAATGATCAAGATTCGTCGCCACTTACACGC
CCACCCCGAGATTTCATTTCATGAAGAACAAACCGCAGCTTACATCAAAGCGTTCTATCGTGAACTGGGCGTCTCAACCA
CTGCTTATGGTGATGGGTACGGCTTCGTCGTCGACATCGATAGCGGTCACCCTGGACCTCAGTTAGCATTACGCGCTGAT
TTTGACGGCCTCGCGATTCAAGAAGACAACCACTTATCATTTAAATCGCAAAACGCCAATGTCATGCACGCCTGTGGTCA
CGATGGTCATACTGCTTATCTCATGGTACTGGCGAAGACCTTACTCGGACTGAAAAATCAGCTACGTGGTCGCGTCCGCA
TTATCCACCAACCCGCTGAAGAAGTATCACCGGGTGGTGCTAAGGGGATGTTGGCTGCTGGCGTCCTAGACGGCATCGAT
AACGTCATTGGGCTGCATGTGATGAGTACCATGCCGACGGGCTCGATCGCCTATCACTTAAATGCGACGCAGACCGGGCG
GGCTAACTTTACGGTCACCTTCACGGGTAAGGGTGGGCACGCCTCGATGCCCCACCTCTCAAACGATGCAATCGTTGCTG
GAAGTTACTTTGTGACCGCCTTACAAACCGTTGTTTCCCGCCGGATTGACCCATTTGATACCGGCAGTGTCACCATCGGC
TCATTCGACGGCGTCGGTTCCTTCAATGCGATCAAAGCGCAAGTCACCCTCAAGGGCGATGTACGGATTATGGACGAAAC
CACGCGCCAGACCATCCGTGACCAGATTACTAAAATTGCGAACGGCGTCGGGGCGACTTTCGGTGTCCAAGTTGCACTTG
ATTACGATGATAATTATCCGGTACTCATGAATGATGCAACCCTGACACAGCGCGTCATCACCAGTATTCAAGCCGCTCAA
ATCCCAGAAATCACTAAAATTTTCGACTGCGGCCCCCAAGACCCCTCGGAGGACTTCGCCTATTTTGCACAAGCCAAACC
CAGCACCTTTATCTATGCCGGCTGTCAAACAGCTCCGGGTGAGACCCACCCGCACCACAGCCCTGATTTTATGATGAATG
AAGACTGTTTACTAATTGCAGCCAAAGCGGTGGGAACGGCAGCGCTGGACTACTTAATGAACGCCTCATCAGATCATTAA

Upstream 100 bases:

>100_bases
TAACCGCCATTTCATGCTAAGCTAAAGATTAGTGCAGTACTATCATCTCTCAGCTATATTTATAATATACTCGCTTCTAG
CATCAGAAAGGAAGATTCTA

Downstream 100 bases:

>100_bases
ACACACTAAAGTGGCTACCTTGAATGCATCCATCCAAGGTAGCCACTTATGATTTATTCAAGCCAACCAGGAATTAACTT
GCAGTGAACACCTAATTAAT

Product: aminoacylase

Products: carboxylate; L-amino acid

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MTLQAQLMQQLTAAEPEMIKIRRHLHAHPEISFHEEQTAAYIKAFYRELGVSTTAYGDGYGFVVDIDSGHPGPQLALRAD
FDGLAIQEDNHLSFKSQNANVMHACGHDGHTAYLMVLAKTLLGLKNQLRGRVRIIHQPAEEVSPGGAKGMLAAGVLDGID
NVIGLHVMSTMPTGSIAYHLNATQTGRANFTVTFTGKGGHASMPHLSNDAIVAGSYFVTALQTVVSRRIDPFDTGSVTIG
SFDGVGSFNAIKAQVTLKGDVRIMDETTRQTIRDQITKIANGVGATFGVQVALDYDDNYPVLMNDATLTQRVITSIQAAQ
IPEITKIFDCGPQDPSEDFAYFAQAKPSTFIYAGCQTAPGETHPHHSPDFMMNEDCLLIAAKAVGTAALDYLMNASSDH

Sequences:

>Translated_399_residues
MTLQAQLMQQLTAAEPEMIKIRRHLHAHPEISFHEEQTAAYIKAFYRELGVSTTAYGDGYGFVVDIDSGHPGPQLALRAD
FDGLAIQEDNHLSFKSQNANVMHACGHDGHTAYLMVLAKTLLGLKNQLRGRVRIIHQPAEEVSPGGAKGMLAAGVLDGID
NVIGLHVMSTMPTGSIAYHLNATQTGRANFTVTFTGKGGHASMPHLSNDAIVAGSYFVTALQTVVSRRIDPFDTGSVTIG
SFDGVGSFNAIKAQVTLKGDVRIMDETTRQTIRDQITKIANGVGATFGVQVALDYDDNYPVLMNDATLTQRVITSIQAAQ
IPEITKIFDCGPQDPSEDFAYFAQAKPSTFIYAGCQTAPGETHPHHSPDFMMNEDCLLIAAKAVGTAALDYLMNASSDH
>Mature_398_residues
TLQAQLMQQLTAAEPEMIKIRRHLHAHPEISFHEEQTAAYIKAFYRELGVSTTAYGDGYGFVVDIDSGHPGPQLALRADF
DGLAIQEDNHLSFKSQNANVMHACGHDGHTAYLMVLAKTLLGLKNQLRGRVRIIHQPAEEVSPGGAKGMLAAGVLDGIDN
VIGLHVMSTMPTGSIAYHLNATQTGRANFTVTFTGKGGHASMPHLSNDAIVAGSYFVTALQTVVSRRIDPFDTGSVTIGS
FDGVGSFNAIKAQVTLKGDVRIMDETTRQTIRDQITKIANGVGATFGVQVALDYDDNYPVLMNDATLTQRVITSIQAAQI
PEITKIFDCGPQDPSEDFAYFAQAKPSTFIYAGCQTAPGETHPHHSPDFMMNEDCLLIAAKAVGTAALDYLMNASSDH

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=365, Percent_Identity=30.4109589041096, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: 3.5.1.14

Molecular weight: Translated: 42993; Mature: 42862

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLQAQLMQQLTAAEPEMIKIRRHLHAHPEISFHEEQTAAYIKAFYRELGVSTTAYGDGY
CCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCE
GFVVDIDSGHPGPQLALRADFDGLAIQEDNHLSFKSQNANVMHACGHDGHTAYLMVLAKT
EEEEECCCCCCCCCEEEEECCCCEEEECCCCCEEECCCCCEEEECCCCCCHHHHHHHHHH
LLGLKNQLRGRVRIIHQPAEEVSPGGAKGMLAAGVLDGIDNVIGLHVMSTMPTGSIAYHL
HHHHHHHCCCEEEEEECCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCEEEEE
NATQTGRANFTVTFTGKGGHASMPHLSNDAIVAGSYFVTALQTVVSRRIDPFDTGSVTIG
ECCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCEEEE
SFDGVGSFNAIKAQVTLKGDVRIMDETTRQTIRDQITKIANGVGATFGVQVALDYDDNYP
CCCCCCCCCEEEEEEEEECCEEEECHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCC
VLMNDATLTQRVITSIQAAQIPEITKIFDCGPQDPSEDFAYFAQAKPSTFIYAGCQTAPG
EEECCHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHCCCCCEEEEECCCCCCC
ETHPHHSPDFMMNEDCLLIAAKAVGTAALDYLMNASSDH
CCCCCCCCCCEECCCEEEEEEHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TLQAQLMQQLTAAEPEMIKIRRHLHAHPEISFHEEQTAAYIKAFYRELGVSTTAYGDGY
CCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCE
GFVVDIDSGHPGPQLALRADFDGLAIQEDNHLSFKSQNANVMHACGHDGHTAYLMVLAKT
EEEEECCCCCCCCCEEEEECCCCEEEECCCCCEEECCCCCEEEECCCCCCHHHHHHHHHH
LLGLKNQLRGRVRIIHQPAEEVSPGGAKGMLAAGVLDGIDNVIGLHVMSTMPTGSIAYHL
HHHHHHHCCCEEEEEECCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCEEEEE
NATQTGRANFTVTFTGKGGHASMPHLSNDAIVAGSYFVTALQTVVSRRIDPFDTGSVTIG
ECCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCEEEE
SFDGVGSFNAIKAQVTLKGDVRIMDETTRQTIRDQITKIANGVGATFGVQVALDYDDNYP
CCCCCCCCCEEEEEEEEECCEEEECHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCC
VLMNDATLTQRVITSIQAAQIPEITKIFDCGPQDPSEDFAYFAQAKPSTFIYAGCQTAPG
EEECCHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHCCCCCEEEEECCCCCCC
ETHPHHSPDFMMNEDCLLIAAKAVGTAALDYLMNASSDH
CCCCCCCCCCEECCCEEEEEEHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-acyl-L-amino acid; H2O

Specific reaction: An N-acyl-L-amino acid + H2O = a carboxylate + an L-amino acid

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]