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The map label for this gene is ogt [H]

Identifier: 28379318

GI number: 28379318

Start: 2539200

End: 2539700

Strand: Direct

Name: ogt [H]

Synonym: lp_2848

Alternate gene names: 28379318

Gene position: 2539200-2539700 (Clockwise)

Preceding gene: 28379317

Following gene: 28379319

Centisome position: 76.75

GC content: 49.9

Gene sequence:

>501_bases
ATGCACTTAACATTGACCCAAACAACCATTAACCAGCAAACTTACTGGCTGGGCACAACGCCCACCGGCTTAGCCTTTGT
CGGCCGTGCCAACGGTCCACGCGACGAATGGCAAGCCTTCTTCAAATCCGCTAATATTCATTATGATCAACAAGCTAATC
AACAGGCCTGTCAAGAATTACATGATTACCTAACTGGTCAACGCCAGCATTTTGACATACCCCTCGACCAAACGACGGGC
ACCCCTTTACAACACCAAGTTTGGCAAGCTTTAACCCGTATTCCATACGGTCAAACTGCAACTTATTCACAACTCGCCAC
AATGATCAATCACCCTACCGCAGTCCGAGCTGTCGCCAGCGCCGTTGGTCAAAATCCACTCCTTATTGTGATCCCCTGCC
ATCGTATCCGCCGCAAAGACGGTCAACTTGGCGGCTATCGTGGTGGTTTGCCAATGAAACGCGCCCTATTAGCTCTTGAA
AACGCCCGCTTGCCCAAATAA

Upstream 100 bases:

>100_bases
TTTCATTAAGATATAGCAGTGATGTTCTGATTTAATCAGGTCGTCACACCACCAATTCTGTTATACTAACGCTATCCTAA
TTGAGACGGAGGGCTCACAC

Downstream 100 bases:

>100_bases
GCTTCACTTAGAACCAAGCGCACGCCCTTGTCACACCGAATCAACTATGTCATCATAAAATCAAACAATGATTGCTAATT
GTGCCAATAGTTATTAGGCC

Product: methylated-DNA--[protein]-cysteine S-methyltransferase

Products: NA

Alternate protein names: O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 166; Mature: 166

Protein sequence:

>166_residues
MHLTLTQTTINQQTYWLGTTPTGLAFVGRANGPRDEWQAFFKSANIHYDQQANQQACQELHDYLTGQRQHFDIPLDQTTG
TPLQHQVWQALTRIPYGQTATYSQLATMINHPTAVRAVASAVGQNPLLIVIPCHRIRRKDGQLGGYRGGLPMKRALLALE
NARLPK

Sequences:

>Translated_166_residues
MHLTLTQTTINQQTYWLGTTPTGLAFVGRANGPRDEWQAFFKSANIHYDQQANQQACQELHDYLTGQRQHFDIPLDQTTG
TPLQHQVWQALTRIPYGQTATYSQLATMINHPTAVRAVASAVGQNPLLIVIPCHRIRRKDGQLGGYRGGLPMKRALLALE
NARLPK
>Mature_166_residues
MHLTLTQTTINQQTYWLGTTPTGLAFVGRANGPRDEWQAFFKSANIHYDQQANQQACQELHDYLTGQRQHFDIPLDQTTG
TPLQHQVWQALTRIPYGQTATYSQLATMINHPTAVRAVASAVGQNPLLIVIPCHRIRRKDGQLGGYRGGLPMKRALLALE
NARLPK

Specific function: Repair of alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction:the enzyme is irreversibly inactivated [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=80, Percent_Identity=43.75, Blast_Score=73, Evalue=1e-13,
Organism=Escherichia coli, GI1788542, Length=125, Percent_Identity=46.4, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI1787596, Length=138, Percent_Identity=35.5072463768116, Blast_Score=91, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI115533068, Length=84, Percent_Identity=47.6190476190476, Blast_Score=82, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI115533070, Length=84, Percent_Identity=47.6190476190476, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6320001, Length=85, Percent_Identity=38.8235294117647, Blast_Score=74, Evalue=9e-15,
Organism=Drosophila melanogaster, GI17137554, Length=141, Percent_Identity=37.5886524822695, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 18613; Mature: 18613

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLTLTQTTINQQTYWLGTTPTGLAFVGRANGPRDEWQAFFKSANIHYDQQANQQACQEL
CEEEEEEEECCCEEEEECCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHH
HDYLTGQRQHFDIPLDQTTGTPLQHQVWQALTRIPYGQTATYSQLATMINHPTAVRAVAS
HHHHCCCCEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHH
AVGQNPLLIVIPCHRIRRKDGQLGGYRGGLPMKRALLALENARLPK
HHCCCCEEEEECCHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MHLTLTQTTINQQTYWLGTTPTGLAFVGRANGPRDEWQAFFKSANIHYDQQANQQACQEL
CEEEEEEEECCCEEEEECCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHH
HDYLTGQRQHFDIPLDQTTGTPLQHQVWQALTRIPYGQTATYSQLATMINHPTAVRAVAS
HHHHCCCCEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHH
AVGQNPLLIVIPCHRIRRKDGQLGGYRGGLPMKRALLALENARLPK
HHCCCCEEEEECCHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2120677; 9384377; 1744039 [H]