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The map label for this gene is cysL [H]

Identifier: 28379313

GI number: 28379313

Start: 2532272

End: 2533099

Strand: Direct

Name: cysL [H]

Synonym: lp_2842

Alternate gene names: 28379313

Gene position: 2532272-2533099 (Clockwise)

Preceding gene: 28379310

Following gene: 28379316

Centisome position: 76.54

GC content: 49.03

Gene sequence:

>828_bases
ATGTTTAAACAATTGGAAACGTTCTGTGCGGTATACGAAACTCGCAACTTCTCCCATGCCGCCGAGCAACTTTTTATTTC
CCAGCCGACCGTCTCAACACAAATCAAACAACTTGAAGCCGATTTACAAACCACGCTCTTAACACGTAACGGGCGCCAAG
AAATCGTTCCTACTGCCAGTGGTCAGCTCCTCTATCAACAGGCTCAAAAGCTGTTAACAACTTGGGCCGCTACCAAAACA
GCGGTACAATCACCACGCCACTTAGTGAAAGTTCCTTGCCGGATTGGTGCCTCACACACAACCGGGCGTCTGATTCTACC
CACCTTATTAGCCAATTTGAGTGCCGTCAGTGATCGCTTTGACTTTCAAGTGACGCTTGCCAATTCGGCCGAAATTTTGA
CCGCTATGTCCCAACACAAACTGGACTTTGGTTTAGTTGAAAAGCCACTCGTTACTGACCAGCTCAATCGCTTGCCATTT
GGTCACGATGAGCTCGTCCATGCGGGCGACTTTGCCAGTCCCCTCTGGCTGCTACGTGAACCTAATTCCGGTGTGCGTCA
TTATACCAACGCCTTTTTAAAAGCCCGCAACATTCAGCCAGCACAAGTCATGGTCATCCACAGTAACCAAATCATCGCGG
ACCTCCTCGCTCATGGTATCGGCCAAACAATTATTTCACGTCACGTTTTAAGCAACGAGATTCCCATCGAACAGCTAAGT
GCCCATTACCAACGCCAATTCTTTTTACTGACTAACACGCAACCGGCAGCGGCACTGCGCCCCGTACAGACTGCAATTAC
CCAGTACCTAACAACGTGGTCAGATTAG

Upstream 100 bases:

>100_bases
CTATCTCGATGCTTTTAAGTATACGAAAGTTATTTACATTTGAGAAATAAATTATTATGATAGTTACTATTAATAAAAAT
AATAGTTAGGGGTGAACACA

Downstream 100 bases:

>100_bases
CGATCCCCGCCGTTGTTATTAGGTCTTTTTGACATTAGCGTTTAAAAAATGGTCTGACCACTAAAGTCAGACCATTTTTG
ATATTCACTGAACAACCGGC

Product: transcription regulator

Products: NA

Alternate protein names: CysJI operon transcriptional activator [H]

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MFKQLETFCAVYETRNFSHAAEQLFISQPTVSTQIKQLEADLQTTLLTRNGRQEIVPTASGQLLYQQAQKLLTTWAATKT
AVQSPRHLVKVPCRIGASHTTGRLILPTLLANLSAVSDRFDFQVTLANSAEILTAMSQHKLDFGLVEKPLVTDQLNRLPF
GHDELVHAGDFASPLWLLREPNSGVRHYTNAFLKARNIQPAQVMVIHSNQIIADLLAHGIGQTIISRHVLSNEIPIEQLS
AHYQRQFFLLTNTQPAAALRPVQTAITQYLTTWSD

Sequences:

>Translated_275_residues
MFKQLETFCAVYETRNFSHAAEQLFISQPTVSTQIKQLEADLQTTLLTRNGRQEIVPTASGQLLYQQAQKLLTTWAATKT
AVQSPRHLVKVPCRIGASHTTGRLILPTLLANLSAVSDRFDFQVTLANSAEILTAMSQHKLDFGLVEKPLVTDQLNRLPF
GHDELVHAGDFASPLWLLREPNSGVRHYTNAFLKARNIQPAQVMVIHSNQIIADLLAHGIGQTIISRHVLSNEIPIEQLS
AHYQRQFFLLTNTQPAAALRPVQTAITQYLTTWSD
>Mature_275_residues
MFKQLETFCAVYETRNFSHAAEQLFISQPTVSTQIKQLEADLQTTLLTRNGRQEIVPTASGQLLYQQAQKLLTTWAATKT
AVQSPRHLVKVPCRIGASHTTGRLILPTLLANLSAVSDRFDFQVTLANSAEILTAMSQHKLDFGLVEKPLVTDQLNRLPF
GHDELVHAGDFASPLWLLREPNSGVRHYTNAFLKARNIQPAQVMVIHSNQIIADLLAHGIGQTIISRHVLSNEIPIEQLS
AHYQRQFFLLTNTQPAAALRPVQTAITQYLTTWSD

Specific function: Transcriptional activator of the cysJI operon which is involved in sulfur assimilation. Also negatively regulates its own transcription [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788481, Length=253, Percent_Identity=26.8774703557312, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1790399, Length=206, Percent_Identity=26.6990291262136, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI48994962, Length=162, Percent_Identity=31.4814814814815, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI157672245, Length=158, Percent_Identity=29.1139240506329, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788887, Length=254, Percent_Identity=25.1968503937008, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 30726; Mature: 30726

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKQLETFCAVYETRNFSHAAEQLFISQPTVSTQIKQLEADLQTTLLTRNGRQEIVPTAS
CCHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
GQLLYQQAQKLLTTWAATKTAVQSPRHLVKVPCRIGASHTTGRLILPTLLANLSAVSDRF
CHHHHHHHHHHHHHHHHHHHHHHCCHHHEECHHHCCCCCCCCCCHHHHHHHHHHHHCCCC
DFQVTLANSAEILTAMSQHKLDFGLVEKPLVTDQLNRLPFGHDELVHAGDFASPLWLLRE
EEEEEECCCHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCHHHEECCCCCCCEEEEEC
PNSGVRHYTNAFLKARNIQPAQVMVIHSNQIIADLLAHGIGQTIISRHVLSNEIPIEQLS
CCCCHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
AHYQRQFFLLTNTQPAAALRPVQTAITQYLTTWSD
HHHCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFKQLETFCAVYETRNFSHAAEQLFISQPTVSTQIKQLEADLQTTLLTRNGRQEIVPTAS
CCHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
GQLLYQQAQKLLTTWAATKTAVQSPRHLVKVPCRIGASHTTGRLILPTLLANLSAVSDRF
CHHHHHHHHHHHHHHHHHHHHHHCCHHHEECHHHCCCCCCCCCCHHHHHHHHHHHHCCCC
DFQVTLANSAEILTAMSQHKLDFGLVEKPLVTDQLNRLPFGHDELVHAGDFASPLWLLRE
EEEEEECCCHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCHHHEECCCCCCCEEEEEC
PNSGVRHYTNAFLKARNIQPAQVMVIHSNQIIADLLAHGIGQTIISRHVLSNEIPIEQLS
CCCCHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
AHYQRQFFLLTNTQPAAALRPVQTAITQYLTTWSD
HHHCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]