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The map label for this gene is ycnB [H]

Identifier: 28379309

GI number: 28379309

Start: 2528457

End: 2529941

Strand: Direct

Name: ycnB [H]

Synonym: lp_2836

Alternate gene names: 28379309

Gene position: 2528457-2529941 (Clockwise)

Preceding gene: 28379305

Following gene: 28379310

Centisome position: 76.43

GC content: 46.94

Gene sequence:

>1485_bases
ATGCCAACTCAAAATTCAACGTTGCCGCTTGATGCCAACGGCCAGCCCTATCACCGGACTTGGCTTGTATTGACTTTACT
TGTCGGGACGTTCACTACCTTCATTACCCAAACACTATTAACGACAGCTTTTCCAACGTTAATGGCCGATTTTCATATTT
CAGCAACCGCCGTTCAGTGGTTGACCACTGGTTTCATGTTAATCATGGGAATCATGATTCCCGTCAGTGCCTGGTTGCTG
ACCCGAATCAATTCCAAGTATTTGTACTTAACAGCCATGCTTATTTTTGGTCTGGGAACTACGCTGGCGTACTTTGCCGT
CAACTTTCAGATGTTGCTGATTGCCCGAATGATTCAAGCAATGGGCGTCGGAATTTCCGCCCCAGTGTTTCAAACAATCA
TGTCTTCAGTTTATCCACCCGAAAAACGGGGTTCCGCAATGGGGACAGCCGGAATTGTTATTGGCTTAGCACCCGCCATC
GGACCGACACTCTCTGGCTGGATTATTGATAACTATTCCTGGCGGGCACTCTTCTTATTCATCCTGCCAATCAATATTCT
AGTCATCATCGTTTCATTCTTCACTTTACGGAAAGTCTTACCAACTTCCAAACAACCAATTGATTGGTGGTCAGTCTTGA
TCTCAACAATTGGTTTTGGCAGTATGTTATACGGTTTTTCATCCGTTGGTAATGCTGGTTGGGGCGACCCAGTCGTCCTC
ACAACTTTAGTCATTGGTCTGATTTTCATTGGTCTATTCGTCTGGCGGCAACTTCACATGGATCAGCCATTATTGGAATT
ACGTGTCTTTAAGATTCCGGCTTTCACCCTATCCGCAATCCTGACCGCCCTCGCTTACATGGCTATGATGGGCGTCGAAA
TGATTTTGCCAATGTATATTCAAACGGTCCTCGGTCGTTCAGCAATGGACTCTGGATTGATTTTATTACCTGGCGCAATC
ATGATGGGAATTATGAGCCCCATTACTGGTCGCATCTTTGACCATATCGGTGCCCGCAGACTAGCAATGACGGGGATGTT
ACTCCTAACGCTTGGAACTGCTTTCTTCCTATCAATTAGCGCCACGACCCCAATCTTATGGATCATCGTTGTTTACGCCA
TTCGGATGTTTGGCCTGACCATGGTTACCATGCCCGTCACAACGACCGGGATCAATGCGTTACCTTTCCGGCTGATTTCC
CATGGGACCGCCGCCAACAACACCCTACGTCAAGTCGCAGCTTCAATGGGGACGGCCGTTTTAATCAGTGTCTACTCCAG
CGTCGCTAAGTGGCAAATGCCGGGACATCAATTACTGACACAGGCACCTTTGAAATACCGTCAGGACGGAATCACCGCAA
CCTTAACTGGTTATCACATGGCGTTCGTTGTCTCACTCATTTTTTGTGCGATTGGCTTTGGGATTACCTTCTTCTTAAAA
GCCAATCATTCAAAAACCATTAGTCAGGAGGATGATGCCGCATGA

Upstream 100 bases:

>100_bases
GCAGACCGCCCCAGTACCAATTAATAGGCCTAGGACTTGCATCGTGATATACTTATTGATTGTGCATTATTACAGAATTT
ATAAAAGAAAGGACGGTATT

Downstream 100 bases:

>100_bases
TCATTGTCTTACTAATTCTTGCAACCCTTGTCTTTGCTGGCACGATGATTTTTTCACCAGCAGGCCGCAACCGTCTCATT
GCCATTATTATTAGTGGCCT

Product: multidrug transport protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MPTQNSTLPLDANGQPYHRTWLVLTLLVGTFTTFITQTLLTTAFPTLMADFHISATAVQWLTTGFMLIMGIMIPVSAWLL
TRINSKYLYLTAMLIFGLGTTLAYFAVNFQMLLIARMIQAMGVGISAPVFQTIMSSVYPPEKRGSAMGTAGIVIGLAPAI
GPTLSGWIIDNYSWRALFLFILPINILVIIVSFFTLRKVLPTSKQPIDWWSVLISTIGFGSMLYGFSSVGNAGWGDPVVL
TTLVIGLIFIGLFVWRQLHMDQPLLELRVFKIPAFTLSAILTALAYMAMMGVEMILPMYIQTVLGRSAMDSGLILLPGAI
MMGIMSPITGRIFDHIGARRLAMTGMLLLTLGTAFFLSISATTPILWIIVVYAIRMFGLTMVTMPVTTTGINALPFRLIS
HGTAANNTLRQVAASMGTAVLISVYSSVAKWQMPGHQLLTQAPLKYRQDGITATLTGYHMAFVVSLIFCAIGFGITFFLK
ANHSKTISQEDDAA

Sequences:

>Translated_494_residues
MPTQNSTLPLDANGQPYHRTWLVLTLLVGTFTTFITQTLLTTAFPTLMADFHISATAVQWLTTGFMLIMGIMIPVSAWLL
TRINSKYLYLTAMLIFGLGTTLAYFAVNFQMLLIARMIQAMGVGISAPVFQTIMSSVYPPEKRGSAMGTAGIVIGLAPAI
GPTLSGWIIDNYSWRALFLFILPINILVIIVSFFTLRKVLPTSKQPIDWWSVLISTIGFGSMLYGFSSVGNAGWGDPVVL
TTLVIGLIFIGLFVWRQLHMDQPLLELRVFKIPAFTLSAILTALAYMAMMGVEMILPMYIQTVLGRSAMDSGLILLPGAI
MMGIMSPITGRIFDHIGARRLAMTGMLLLTLGTAFFLSISATTPILWIIVVYAIRMFGLTMVTMPVTTTGINALPFRLIS
HGTAANNTLRQVAASMGTAVLISVYSSVAKWQMPGHQLLTQAPLKYRQDGITATLTGYHMAFVVSLIFCAIGFGITFFLK
ANHSKTISQEDDAA
>Mature_493_residues
PTQNSTLPLDANGQPYHRTWLVLTLLVGTFTTFITQTLLTTAFPTLMADFHISATAVQWLTTGFMLIMGIMIPVSAWLLT
RINSKYLYLTAMLIFGLGTTLAYFAVNFQMLLIARMIQAMGVGISAPVFQTIMSSVYPPEKRGSAMGTAGIVIGLAPAIG
PTLSGWIIDNYSWRALFLFILPINILVIIVSFFTLRKVLPTSKQPIDWWSVLISTIGFGSMLYGFSSVGNAGWGDPVVLT
TLVIGLIFIGLFVWRQLHMDQPLLELRVFKIPAFTLSAILTALAYMAMMGVEMILPMYIQTVLGRSAMDSGLILLPGAIM
MGIMSPITGRIFDHIGARRLAMTGMLLLTLGTAFFLSISATTPILWIIVVYAIRMFGLTMVTMPVTTTGINALPFRLISH
GTAANNTLRQVAASMGTAVLISVYSSVAKWQMPGHQLLTQAPLKYRQDGITATLTGYHMAFVVSLIFCAIGFGITFFLKA
NHSKTISQEDDAA

Specific function: Translocase That Confers Resistance To Substances Of High Hydrophobicity. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=450, Percent_Identity=26.6666666666667, Blast_Score=150, Evalue=3e-37,
Organism=Escherichia coli, GI1788710, Length=412, Percent_Identity=26.4563106796117, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI87081983, Length=422, Percent_Identity=25.1184834123223, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1790195, Length=404, Percent_Identity=23.5148514851485, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788392, Length=412, Percent_Identity=21.8446601941748, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI48994889, Length=253, Percent_Identity=27.6679841897233, Blast_Score=73, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6323735, Length=335, Percent_Identity=26.2686567164179, Blast_Score=79, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 53929; Mature: 53798

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
6.1 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
5.9 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTQNSTLPLDANGQPYHRTWLVLTLLVGTFTTFITQTLLTTAFPTLMADFHISATAVQW
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTTGFMLIMGIMIPVSAWLLTRINSKYLYLTAMLIFGLGTTLAYFAVNFQMLLIARMIQA
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MGVGISAPVFQTIMSSVYPPEKRGSAMGTAGIVIGLAPAIGPTLSGWIIDNYSWRALFLF
HCCCCCHHHHHHHHHHCCCCHHCCCCCCHHHHHHEEHHHCCCCCCCEEECCCHHHHHHHH
ILPINILVIIVSFFTLRKVLPTSKQPIDWWSVLISTIGFGSMLYGFSSVGNAGWGDPVVL
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
TTLVIGLIFIGLFVWRQLHMDQPLLELRVFKIPAFTLSAILTALAYMAMMGVEMILPMYI
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QTVLGRSAMDSGLILLPGAIMMGIMSPITGRIFDHIGARRLAMTGMLLLTLGTAFFLSIS
HHHHCCHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ATTPILWIIVVYAIRMFGLTMVTMPVTTTGINALPFRLISHGTAANNTLRQVAASMGTAV
CCHHHHHHHHHHHHHHHHHHHHEECCHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHH
LISVYSSVAKWQMPGHQLLTQAPLKYRQDGITATLTGYHMAFVVSLIFCAIGFGITFFLK
HHHHHHHHHHHCCCHHHHHHHCCHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHEEEEE
ANHSKTISQEDDAA
CCCCCCCCCCCCCC
>Mature Secondary Structure 
PTQNSTLPLDANGQPYHRTWLVLTLLVGTFTTFITQTLLTTAFPTLMADFHISATAVQW
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTTGFMLIMGIMIPVSAWLLTRINSKYLYLTAMLIFGLGTTLAYFAVNFQMLLIARMIQA
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MGVGISAPVFQTIMSSVYPPEKRGSAMGTAGIVIGLAPAIGPTLSGWIIDNYSWRALFLF
HCCCCCHHHHHHHHHHCCCCHHCCCCCCHHHHHHEEHHHCCCCCCCEEECCCHHHHHHHH
ILPINILVIIVSFFTLRKVLPTSKQPIDWWSVLISTIGFGSMLYGFSSVGNAGWGDPVVL
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
TTLVIGLIFIGLFVWRQLHMDQPLLELRVFKIPAFTLSAILTALAYMAMMGVEMILPMYI
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QTVLGRSAMDSGLILLPGAIMMGIMSPITGRIFDHIGARRLAMTGMLLLTLGTAFFLSIS
HHHHCCHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ATTPILWIIVVYAIRMFGLTMVTMPVTTTGINALPFRLISHGTAANNTLRQVAASMGTAV
CCHHHHHHHHHHHHHHHHHHHHEECCHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHH
LISVYSSVAKWQMPGHQLLTQAPLKYRQDGITATLTGYHMAFVVSLIFCAIGFGITFFLK
HHHHHHHHHHHCCCHHHHHHHCCHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHEEEEE
ANHSKTISQEDDAA
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]